P0CX32 (RS24B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S24-B

Alternative name(s):
RP50

Gene names

Name:	RPS24B
Synonyms:	RPS24EB
Ordered Locus Names:	YIL069C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.5.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA. Also partially acetylated by NatC. HAMAP-Rule MF_00545
Miscellaneous	Present with 6160 molecules/cell in log phase SD medium. HAMAP-Rule MF_00545 There are 2 genes for S24 in yeast. HAMAP-Rule MF_00545
Sequence similarities	Belongs to the ribosomal protein S24e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Non-traceable author statement Ref.4. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction PubMed 16246728. Source: SGD translational elongation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosolic small ribosomal subunit Inferred from Biological aspect of Ancestor. Source: RefGenome nucleolus Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Non-traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 135	134	40S ribosomal protein S24-B HAMAP-Rule MF_00545		PRO_0000409762
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.3
	Modified residue	14	1	Phosphoserine Ref.7 Ref.8
Experimental info
	Sequence conflict	16	1	P → D AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P0CX32 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 81B82D361207442A	FASTA	135	15,329
	10 20 30 40 50 60 MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF 70 80 90 100 110 120 RTQFGGGKSV GFGLVYNSVA EAKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG 130 TGKRLAKKVA RRNAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16, ACETYLATION AT SER-2 BY NATA.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38060 Genomic DNA. Translation: CAA86154.1. BK006942 Genomic DNA. Translation: DAA08481.1.
PIR	S48410.
RefSeq	NP_010997.3. NM_001178965.3. NP_012195.1. NM_001179419.1.
3D structure databases
ProteinModelPortal	P0CX32.
SMR	P0CX32. Positions 2-135.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER074W; YER074W; YER074W. YIL069C; YIL069C; YIL069C.
GeneID	854741. 856805.
KEGG	sce:YER074W. sce:YER076C. sce:YIL069C.
Organism-specific databases
CYGD	YIL069c.
SGD	S000001331. RPS24B.
Phylogenomic databases
GeneTree	ENSGT00390000000153.
KO	K02974.
Gene expression databases
ArrayExpress	P0CX32.
GermOnline	YIL069C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
HAMAP	MF_00545. Ribosomal_S24e.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR012678. Ribosomal_L23/L15e_core_dom. IPR001976. Ribosomal_S24e. IPR018098. Ribosomal_S24e_CS. [Graphical view]
PANTHER	PTHR10496. PTHR10496. 1 hit.
Pfam	PF01282. Ribosomal_S24e. 1 hit. [Graphical view]
ProDom	PD006052. Ribosomal_S24e. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54189. L23_L15e_core. 1 hit.
PROSITE	PS00529. RIBOSOMAL_S24E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977455.

Entry information

Entry name

RS24B_YEAST

Accession

Primary (citable) accession number: P0CX32
Secondary accession number(s): D3DLX9, P26782

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program