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P0CX29 (RS23A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S23-A
Alternative name(s):
RP37
S28
YS14
Gene names
Name:RPS23A
Synonyms:RPS28A
Ordered Locus Names:YGR118W
ORF Names:G6178
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length145 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5

Subcellular location

Cytoplasm Ref.6.

Post-translational modification

Hydroxylation at Pro-64 affects translation termination efficiency. The stereochemistry of the 3,4-dihydroxyproline has not yet been determined.

Miscellaneous

Present with 10600 molecules/cell in log phase SD medium.

There are 2 genes for S23 in yeast.

Sequence similarities

Belongs to the ribosomal protein S12P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14514540S ribosomal protein S23-A
PRO_0000146482

Amino acid modifications

Modified residue6413,4-dihydroxyproline Ref.9

Experimental info

Mutagenesis641P → A: Lethal mutation. Ref.9
Mutagenesis651N → A: Lethal mutation. Ref.9

Secondary structure

.............................. 145
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0CX29 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 5A89ADF540DA1311

FASTA14516,038
        10         20         30         40         50         60 
MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE 

        70         80         90        100        110        120 
SKQPNSAIRK CVRVQLIKNG KKVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV 

       130        140 
RFKVVKVSGV SLLALWKEKK EKPRS 

« Hide

References

« Hide 'large scale' references
[1]"A novel cloning strategy reveals the gene for the yeast homologue to Escherichia coli ribosomal protein S12."
Alksne L.E., Warner J.R.
J. Biol. Chem. 268:10813-10819(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 23.4 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals CLB6, SPT6, RP28A and NUP57 genes, a Ty3 element and 11 new open reading frames."
Hansen M., Albers M., Backes U., Coblenz A., Leuther H., Neu R., Schreer A., Schaefer B., Zimmermann M., Wolf K.
Yeast 12:1273-1277(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Hydroxylation of the eukaryotic ribosomal decoding center affects translational accuracy."
Loenarz C., Sekirnik R., Thalhammer A., Ge W., Spivakovsky E., Mackeen M.M., McDonough M.A., Cockman M.E., Kessler B.M., Ratcliffe P.J., Wolf A., Schofield C.J.
Proc. Natl. Acad. Sci. U.S.A. 111:4019-4024(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: HYDROXYLATION AT PRO-64, MUTAGENESIS OF PRO-64 AND ASN-65.
[10]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 28-145, ELECTRON MICROSCOPY.
[11]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 28-145, ELECTRON MICROSCOPY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96570 Unassigned RNA. Translation: AAA16235.1.
Z72903 Genomic DNA. Translation: CAA97128.1.
BK006941 Genomic DNA. Translation: DAA08210.1.
PIRA46703.
RefSeqNP_011633.3. NM_001181247.3.
NP_015457.1. NM_001184229.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-L28-145[»]
1S1Helectron microscopy11.70L28-145[»]
3IZBelectron microscopy-L1-145[»]
3JYVelectron microscopy8.90L28-145[»]
3O2ZX-ray4.00P1-145[»]
3O30X-ray4.00P1-145[»]
3U5CX-ray3.00X1-145[»]
3U5GX-ray3.00X1-145[»]
4BYLelectron microscopy4.30X1-145[»]
4BYTelectron microscopy6.60X1-145[»]
ProteinModelPortalP0CX29.
SMRP0CX29. Positions 2-145.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33363. 92 interactions.
36298. 23 interactions.

Proteomic databases

MaxQBP0CX29.
PRIDEP0CX29.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR118W; YGR118W; YGR118W.
YPR132W; YPR132W; YPR132W.
GeneID853015.
856250.
KEGGsce:YGR118W.
sce:YPR132W.

Organism-specific databases

CYGDYGR118w.
SGDS000003350. RPS23A.

Phylogenomic databases

KOK02973.
OrthoDBEOG7SN8R7.

Enzyme and pathway databases

BioCycYEAST:G3O-30825-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
InterProIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005680. Ribosomal_S23_euk/arc.
[Graphical view]
PANTHERPTHR11652. PTHR11652. 1 hit.
PfamPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFPIRSF002133. Ribosomal_S12/S23. 1 hit.
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00982. S23_S12_E_A. 1 hit.
PROSITEPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0CX29.
NextBio972878.

Entry information

Entry nameRS23A_YEAST
AccessionPrimary (citable) accession number: P0CX29
Secondary accession number(s): D6VUP9, P32827
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references