P0CX29 (RS23A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S23-A

Alternative name(s):
RP37
S28
YS14

Gene names

Name:	RPS23A
Synonyms:	RPS28A
Ordered Locus Names:	YGR118W
ORF Names:	G6178

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 10600 molecules/cell in log phase SD medium. There are 2 genes for S23 in yeast.
Sequence similarities	Belongs to the ribosomal protein S12P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction PubMed 16246728. Source: SGD regulation of translational fidelity Inferred from mutant phenotype PubMed 7498767PubMed 8415737PubMed 8950190. Source: SGD translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay PubMed 6814480. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay PubMed 6814480. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 145

145

40S ribosomal protein S23-A

PRO_0000146482

Amino acid modifications

Modified residue

128

1

Phosphoserine Ref.8

Secondary structure

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145

Helix Strand Turn

Details...

Helix

12 – 23

12

Helix

27 – 34

8

Helix

36 – 39

4

Beta strand

40 – 43

4

Beta strand

45 – 58

14

Beta strand

71 – 76

6

Turn

77 – 79

3

Beta strand

82 – 86

5

Beta strand

89 – 91

3

Helix

93 – 95

3

Beta strand

101 – 105

5

Beta strand

108 – 112

5

Beta strand

115 – 117

3

Beta strand

122 – 127

6

Turn

128 – 130

3

Helix

132 – 137

6

Sequences

	Sequence		Length	Mass (Da)
	P0CX29 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 5A89ADF540DA1311	FASTA	145	16,038
	10 20 30 40 50 60 MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE 70 80 90 100 110 120 SKQPNSAIRK CVRVQLIKNG KKVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV 130 140 RFKVVKVSGV SLLALWKEKK EKPRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel cloning strategy reveals the gene for the yeast homologue to Escherichia coli ribosomal protein S12." Alksne L.E., Warner J.R. J. Biol. Chem. 268:10813-10819(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 23.4 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals CLB6, SPT6, RP28A and NUP57 genes, a Ty3 element and 11 new open reading frames." Hansen M., Albers M., Backes U., Coblenz A., Leuther H., Neu R., Schreer A., Schaefer B., Zimmermann M., Wolf K. Yeast 12:1273-1277(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128, MASS SPECTROMETRY.
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 28-145, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 28-145, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M96570 Unassigned RNA. Translation: AAA16235.1.
Z72903 Genomic DNA. Translation: CAA97128.1.
BK006941 Genomic DNA. Translation: DAA08210.1.

PIR

A46703.

RefSeq

NP_011633.3. NM_001181247.3.
NP_015457.1. NM_001184229.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	L	28-145	[»]
1S1H	electron microscopy	11.70	L	28-145	[»]
3IZB	electron microscopy	-	L	1-145	[»]
3O2Z	X-ray	4.00	P	1-145	[»]
3O30	X-ray	4.00	P	1-145	[»]
3U5C	X-ray	3.00	X	1-145	[»]
3U5G	X-ray	3.00	X	1-145	[»]

ProteinModelPortal

P0CX29.

SMR

P0CX29. Positions 2-145.

ModBase

Search...

Proteomic databases

PRIDE

P0CX29.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR118W; YGR118W; YGR118W.
YPR132W; YPR132W; YPR132W.

GeneID

853015.
856250.

KEGG

sce:YGR118W.
sce:YGR123C.
sce:YPR132W.

Organism-specific databases

CYGD

YGR118w.

SGD

S000003350. RPS23A.

Phylogenomic databases

KO

K02973.
K04460.

Gene expression databases

ArrayExpress

P0CX29.

GermOnline

YGR118W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.40.50.140. 1 hit.

InterPro

IPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005680. Ribosomal_S23_euk/arc.
[Graphical view]

PANTHER

PTHR11652. PTHR11652. 1 hit.

Pfam

PF00164. Ribosomal_S12. 1 hit.
[Graphical view]

PIRSF

PIRSF002133. Ribosomal_S12/S23. 1 hit.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

TIGRFAMs

TIGR00982. S23_S12_E_A. 1 hit.

PROSITE

PS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX29.

NextBio

972878.

Entry information

Entry name

RS23A_YEAST

Accession

Primary (citable) accession number: P0CX29
Secondary accession number(s): D6VUP9, P32827

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program