P0CX28 (RL44B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L42-B

Alternative name(s):
L41
Maintenance of killer protein 18
YL27
YP44

Gene names

Name:	RPL42B
Synonyms:	MAK18, RPL41B, SCL41B
Ordered Locus Names:	YHR141C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.8.
Post-translational modification	In wild-type cells, 78% of L42 is monomethylated at both Lys-40 and Lys-55, and 22% are a mixture of species with either residue monomethylated. Ref.10
Miscellaneous	Present with 3890 molecules/cell in log phase SD medium. There are 2 genes for L42 in yeast.
Sequence similarities	Belongs to the ribosomal protein L44e family.
Mass spectrometry	Molecular mass is 12100.729 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with 2 methylation modifications. Ref.7 Molecular mass is 12100.71 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55. Ref.10 Molecular mass is 12108.0 Da from positions 2 - 106. Determined by ESI. With N6-methyl-Lys-40 and N6-methyl-Lys-55. Ref.10

Ontologies

Keywords
Biological process	Antibiotic resistance Cycloheximide resistance
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Methylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.6. Source: SGD response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to cycloheximide Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.6. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 106	105	60S ribosomal protein L42-B		PRO_0000409760
Amino acid modifications
	Modified residue	40	1	N6-methyllysine; by RKM3 Ref.10
	Modified residue	55	1	N6-methyllysine; by RKM4 Ref.10
Natural variations
	Natural variant	56	1	P → Q Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. Ref.1
Experimental info
	Sequence conflict	40 – 41	2	KR → RK AA sequence Ref.4
	Sequence conflict	88 – 89	2	Missing AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P0CX28 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 730CA11F2CF7F2B4	FASTA	106	12,212
	10 20 30 40 50 60 MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK 70 80 90 100 KAKTTKKVVL RLECVKCKTR AQLTLKRCKH FELGGEKKQK GQALQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts." Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M. J. Bacteriol. 174:254-262(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-56.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The primary structure of protein 44 from the large subunit of yeast ribosomes." Itoh T., Wittmann-Liebold B. FEBS Lett. 96:399-402(1978) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-106.
[5]	"Translation and M1 double-stranded RNA propagation: MAK18 = RPL41B and cycloheximide curing." Carroll K., Wickner R.B. J. Bacteriol. 177:2887-2891(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS MAK18.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR." Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D. Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab." Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G. J. Biol. Chem. 283:35561-35568(2008) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT LYS-40 AND LYS-55, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10579 Genomic DNA. Translation: BAA01436.1. U10398 Genomic DNA. Translation: AAB68420.1. BK006934 Genomic DNA. Translation: DAA06834.1.
PIR	R6BY44. C43301.
RefSeq	NP_012010.1. NM_001179271.1. NP_014237.2. NM_001183000.1.
3D structure databases
ProteinModelPortal	P0CX28.
SMR	P0CX28. Positions 2-106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR141C; YHR141C; YHR141C. YNL162W; YNL162W; YNL162W.
GeneID	855560. 856544.
KEGG	sce:YHR141C. sce:YNL162W.
Organism-specific databases
CYGD	YHR141c.
SGD	S000001183. RPL42B.
Phylogenomic databases
GeneTree	ENSGT00390000018085.
KO	K02929.
Gene expression databases
ArrayExpress	P0CX28.
GermOnline	YHR141C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.10.450.80. 1 hit.
InterPro	IPR000552. Ribosomal_L44e. IPR011332. Ribosomal_zn-bd_dom. [Graphical view]
PANTHER	PTHR10369. PTHR10369. 1 hit.
Pfam	PF00935. Ribosomal_L44. 1 hit. [Graphical view]
ProDom	PD002841. Ribosomal_L44e. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57829. Ribosomal_zn-bd. 1 hit.
PROSITE	PS01172. RIBOSOMAL_L44E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979652.

Entry information

Entry name

RL44B_YEAST

Accession

Primary (citable) accession number: P0CX28
Secondary accession number(s): D3DL90, P02405

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program