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P0CX28

- RL44B_YEAST

UniProt

P0CX28 - RL44B_YEAST

Protein

60S ribosomal protein L42-B

Gene

RPL42B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. response to antibiotic Source: UniProtKB-KW
    3. response to cycloheximide Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Antibiotic resistance, Cycloheximide resistance

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31177-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L42-B
    Alternative name(s):
    L41
    Maintenance of killer protein 18
    YL27
    YP44
    Gene namesi
    Name:RPL42B
    Synonyms:MAK18, RPL41B, SCL41B
    Ordered Locus Names:YHR141C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR141c.
    SGDiS000001183. RPL42B.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 10610560S ribosomal protein L42-BPRO_0000409760Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401N6-methyllysine; by RKM31 Publication
    Modified residuei55 – 551N6-methyllysine; by RKM41 Publication

    Post-translational modificationi

    In wild-type cells, 78% of L42 is monomethylated at both Lys-40 and Lys-55, and 22% are a mixture of species with either residue monomethylated.

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi35667. 28 interactions.
    36574. 62 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CX28.
    SMRiP0CX28. Positions 2-106.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L44e family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000018085.
    KOiK02929.
    OrthoDBiEOG7MWH8Z.

    Family and domain databases

    Gene3Di3.10.450.80. 1 hit.
    InterProiIPR000552. Ribosomal_L44e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PANTHERiPTHR10369. PTHR10369. 1 hit.
    PfamiPF00935. Ribosomal_L44. 1 hit.
    [Graphical view]
    ProDomiPD002841. Ribosomal_L44e. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57829. SSF57829. 1 hit.
    PROSITEiPS01172. RIBOSOMAL_L44E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX28-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF    50
    GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCKH FELGGEKKQK 100
    GQALQF 106
    Length:106
    Mass (Da):12,212
    Last modified:June 28, 2011 - v1
    Checksum:i730CA11F2CF7F2B4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 412KR → RK AA sequence (PubMed:365584)Curated
    Sequence conflicti88 – 892Missing AA sequence (PubMed:365584)Curated

    Mass spectrometryi

    Molecular mass is 12100.729 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with 2 methylation modifications.1 Publication
    Molecular mass is 12100.71 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55.1 Publication
    Molecular mass is 12108.0 Da from positions 2 - 106. Determined by ESI. With N6-methyl-Lys-40 and N6-methyl-Lys-55.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561P → Q Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10579 Genomic DNA. Translation: BAA01436.1.
    U10398 Genomic DNA. Translation: AAB68420.1.
    BK006934 Genomic DNA. Translation: DAA06834.1.
    PIRiC43301. R6BY44.
    RefSeqiNP_012010.1. NM_001179271.1.
    NP_014237.2. NM_001183000.1.

    Genome annotation databases

    EnsemblFungiiYHR141C; YHR141C; YHR141C.
    YNL162W; YNL162W; YNL162W.
    GeneIDi855560.
    856544.
    KEGGisce:YHR141C.
    sce:YNL162W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10579 Genomic DNA. Translation: BAA01436.1 .
    U10398 Genomic DNA. Translation: AAB68420.1 .
    BK006934 Genomic DNA. Translation: DAA06834.1 .
    PIRi C43301. R6BY44.
    RefSeqi NP_012010.1. NM_001179271.1.
    NP_014237.2. NM_001183000.1.

    3D structure databases

    ProteinModelPortali P0CX28.
    SMRi P0CX28. Positions 2-106.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35667. 28 interactions.
    36574. 62 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR141C ; YHR141C ; YHR141C .
    YNL162W ; YNL162W ; YNL162W .
    GeneIDi 855560.
    856544.
    KEGGi sce:YHR141C.
    sce:YNL162W.

    Organism-specific databases

    CYGDi YHR141c.
    SGDi S000001183. RPL42B.

    Phylogenomic databases

    GeneTreei ENSGT00390000018085.
    KOi K02929.
    OrthoDBi EOG7MWH8Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31177-MONOMER.

    Miscellaneous databases

    NextBioi 979652.

    Family and domain databases

    Gene3Di 3.10.450.80. 1 hit.
    InterProi IPR000552. Ribosomal_L44e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    PANTHERi PTHR10369. PTHR10369. 1 hit.
    Pfami PF00935. Ribosomal_L44. 1 hit.
    [Graphical view ]
    ProDomi PD002841. Ribosomal_L44e. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    PROSITEi PS01172. RIBOSOMAL_L44E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts."
      Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M.
      J. Bacteriol. 174:254-262(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-56.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The primary structure of protein 44 from the large subunit of yeast ribosomes."
      Itoh T., Wittmann-Liebold B.
      FEBS Lett. 96:399-402(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-106.
    5. "Translation and M1 double-stranded RNA propagation: MAK18 = RPL41B and cycloheximide curing."
      Carroll K., Wickner R.B.
      J. Bacteriol. 177:2887-2891(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION AS MAK18.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab."
      Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G.
      J. Biol. Chem. 283:35561-35568(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-40 AND LYS-55, MASS SPECTROMETRY.

    Entry informationi

    Entry nameiRL44B_YEAST
    AccessioniPrimary (citable) accession number: P0CX28
    Secondary accession number(s): D3DL90, P02405
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3890 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L42 in yeast.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3