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P0CX28

- RL44B_YEAST

UniProt

P0CX28 - RL44B_YEAST

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Protein

60S ribosomal protein L42-B

Gene

RPL42B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. response to antibiotic Source: UniProtKB-KW
  3. response to cycloheximide Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Antibiotic resistance, Cycloheximide resistance

Enzyme and pathway databases

BioCyciYEAST:G3O-31177-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L42-B
Alternative name(s):
L41
Maintenance of killer protein 18
YL27
YP44
Gene namesi
Name:RPL42B
Synonyms:MAK18, RPL41B, SCL41B
Ordered Locus Names:YHR141C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR141c.
SGDiS000001183. RPL42B.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 10610560S ribosomal protein L42-BPRO_0000409760Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401N6-methyllysine; by RKM33 Publications
Modified residuei55 – 551N6-methyllysine; by RKM42 Publications

Post-translational modificationi

In wild-type cells, 78% of L42 is monomethylated at both Lys-40 and Lys-55, and 22% are a mixture of species with either residue monomethylated.

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi35667. 29 interactions.
36574. 63 interactions.

Structurei

3D structure databases

ProteinModelPortaliP0CX28.
SMRiP0CX28. Positions 2-106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L44e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018085.
InParanoidiP0CX28.
KOiK02929.
OrthoDBiEOG7MWH8Z.

Family and domain databases

Gene3Di3.10.450.80. 1 hit.
InterProiIPR000552. Ribosomal_L44e.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERiPTHR10369. PTHR10369. 1 hit.
PfamiPF00935. Ribosomal_L44. 1 hit.
[Graphical view]
ProDomiPD002841. Ribosomal_L44e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57829. SSF57829. 1 hit.
PROSITEiPS01172. RIBOSOMAL_L44E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX28-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF
60 70 80 90 100
GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCKH FELGGEKKQK

GQALQF
Length:106
Mass (Da):12,212
Last modified:June 28, 2011 - v1
Checksum:i730CA11F2CF7F2B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 412KR → RK AA sequence (PubMed:365584)Curated
Sequence conflicti88 – 892Missing AA sequence (PubMed:365584)Curated

Mass spectrometryi

Molecular mass is 12100.729 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with 2 methylation modifications.1 Publication
Molecular mass is 12100.71 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55.1 Publication
Molecular mass is 12108.0 Da from positions 2 - 106. Determined by ESI. With N6-methyl-Lys-40 and N6-methyl-Lys-55.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti56 – 561P → Q Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10579 Genomic DNA. Translation: BAA01436.1.
U10398 Genomic DNA. Translation: AAB68420.1.
BK006934 Genomic DNA. Translation: DAA06834.1.
PIRiC43301. R6BY44.
RefSeqiNP_012010.1. NM_001179271.1.
NP_014237.2. NM_001183000.1.

Genome annotation databases

EnsemblFungiiYHR141C; YHR141C; YHR141C.
YNL162W; YNL162W; YNL162W.
GeneIDi855560.
856544.
KEGGisce:YHR141C.
sce:YNL162W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10579 Genomic DNA. Translation: BAA01436.1 .
U10398 Genomic DNA. Translation: AAB68420.1 .
BK006934 Genomic DNA. Translation: DAA06834.1 .
PIRi C43301. R6BY44.
RefSeqi NP_012010.1. NM_001179271.1.
NP_014237.2. NM_001183000.1.

3D structure databases

ProteinModelPortali P0CX28.
SMRi P0CX28. Positions 2-106.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35667. 29 interactions.
36574. 63 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR141C ; YHR141C ; YHR141C .
YNL162W ; YNL162W ; YNL162W .
GeneIDi 855560.
856544.
KEGGi sce:YHR141C.
sce:YNL162W.

Organism-specific databases

CYGDi YHR141c.
SGDi S000001183. RPL42B.

Phylogenomic databases

GeneTreei ENSGT00390000018085.
InParanoidi P0CX28.
KOi K02929.
OrthoDBi EOG7MWH8Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-31177-MONOMER.

Miscellaneous databases

NextBioi 979652.

Family and domain databases

Gene3Di 3.10.450.80. 1 hit.
InterProi IPR000552. Ribosomal_L44e.
IPR011332. Ribosomal_zn-bd.
[Graphical view ]
PANTHERi PTHR10369. PTHR10369. 1 hit.
Pfami PF00935. Ribosomal_L44. 1 hit.
[Graphical view ]
ProDomi PD002841. Ribosomal_L44e. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF57829. SSF57829. 1 hit.
PROSITEi PS01172. RIBOSOMAL_L44E. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts."
    Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M.
    J. Bacteriol. 174:254-262(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-56.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The primary structure of protein 44 from the large subunit of yeast ribosomes."
    Itoh T., Wittmann-Liebold B.
    FEBS Lett. 96:399-402(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-106.
  5. "Translation and M1 double-stranded RNA propagation: MAK18 = RPL41B and cycloheximide curing."
    Carroll K., Wickner R.B.
    J. Bacteriol. 177:2887-2891(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS MAK18.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
    Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
    Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab."
    Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G.
    J. Biol. Chem. 283:35561-35568(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-40 AND LYS-55, MASS SPECTROMETRY.
  11. "Methylation of translation-associated proteins in Saccharomyces cerevisiae: Identification of methylated lysines and their methyltransferases."
    Couttas T.A., Raftery M.J., Padula M.P., Herbert B.R., Wilkins M.R.
    Proteomics 12:960-972(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-40.
  12. "Stoichiometry of Saccharomyces cerevisiae lysine methylation: insights into non-histone protein lysine methyltransferase activity."
    Hart-Smith G., Chia S.Z., Low J.K., McKay M.J., Molloy M.P., Wilkins M.R.
    J. Proteome Res. 13:1744-1756(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-40 BY RKM3, METHYLATION AT LYS-55 BY RKM4.

Entry informationi

Entry nameiRL44B_YEAST
AccessioniPrimary (citable) accession number: P0CX28
Secondary accession number(s): D3DL90, P02405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3890 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L42 in yeast.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3