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P0CX28 (RL44B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S ribosomal protein L42-B
Alternative name(s):
L41
Maintenance of killer protein 18
YL27
YP44
Gene names
Name:RPL42B
Synonyms:MAK18, RPL41B, SCL41B
Ordered Locus Names:YHR141C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length106 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6

Subcellular location

Cytoplasm Ref.8.

Post-translational modification

In wild-type cells, 78% of L42 is monomethylated at both Lys-40 and Lys-55, and 22% are a mixture of species with either residue monomethylated. Ref.10

Miscellaneous

Present with 3890 molecules/cell in log phase SD medium.

There are 2 genes for L42 in yeast.

Sequence similarities

Belongs to the ribosomal protein L44e family.

Mass spectrometry

Molecular mass is 12100.729 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with 2 methylation modifications. Ref.7

Molecular mass is 12100.71 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55. Ref.10

Molecular mass is 12108.0 Da from positions 2 - 106. Determined by ESI. With N6-methyl-Lys-40 and N6-methyl-Lys-55. Ref.10

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 10610560S ribosomal protein L42-B
PRO_0000409760

Amino acid modifications

Modified residue401N6-methyllysine; by RKM3 Ref.10
Modified residue551N6-methyllysine; by RKM4 Ref.10

Natural variations

Natural variant561P → Q Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. Ref.1

Experimental info

Sequence conflict40 – 412KR → RK AA sequence Ref.4
Sequence conflict88 – 892Missing AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P0CX28 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 730CA11F2CF7F2B4

FASTA10612,212
        10         20         30         40         50         60 
MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK 

        70         80         90        100 
KAKTTKKVVL RLECVKCKTR AQLTLKRCKH FELGGEKKQK GQALQF 

« Hide

References

« Hide 'large scale' references
[1]"Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts."
Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M.
J. Bacteriol. 174:254-262(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-56.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The primary structure of protein 44 from the large subunit of yeast ribosomes."
Itoh T., Wittmann-Liebold B.
FEBS Lett. 96:399-402(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-106.
[5]"Translation and M1 double-stranded RNA propagation: MAK18 = RPL41B and cycloheximide curing."
Carroll K., Wickner R.B.
J. Bacteriol. 177:2887-2891(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION AS MAK18.
[6]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[7]"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab."
Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G.
J. Biol. Chem. 283:35561-35568(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-40 AND LYS-55, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10579 Genomic DNA. Translation: BAA01436.1.
U10398 Genomic DNA. Translation: AAB68420.1.
BK006934 Genomic DNA. Translation: DAA06834.1.
PIRR6BY44. C43301.
RefSeqNP_012010.1. NM_001179271.1.
NP_014237.2. NM_001183000.1.

3D structure databases

ProteinModelPortalP0CX28.
SMRP0CX28. Positions 2-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35667. 28 interactions.
36574. 62 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR141C; YHR141C; YHR141C.
YNL162W; YNL162W; YNL162W.
GeneID855560.
856544.
KEGGsce:YHR141C.
sce:YNL162W.

Organism-specific databases

CYGDYHR141c.
SGDS000001183. RPL42B.

Phylogenomic databases

GeneTreeENSGT00390000018085.
KOK02929.
OrthoDBEOG7MWH8Z.

Enzyme and pathway databases

BioCycYEAST:G3O-31177-MONOMER.

Family and domain databases

Gene3D3.10.450.80. 1 hit.
InterProIPR000552. Ribosomal_L44e.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERPTHR10369. PTHR10369. 1 hit.
PfamPF00935. Ribosomal_L44. 1 hit.
[Graphical view]
ProDomPD002841. Ribosomal_L44e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF57829. SSF57829. 1 hit.
PROSITEPS01172. RIBOSOMAL_L44E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979652.

Entry information

Entry nameRL44B_YEAST
AccessionPrimary (citable) accession number: P0CX28
Secondary accession number(s): D3DL90, P02405
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries