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P0CX27 (RL44A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S ribosomal protein L42-A
Alternative name(s):
L41
YL27
YP44
Gene names
Name:RPL42A
Synonyms:RPL41A, SCL41A
Ordered Locus Names:YNL162W
ORF Names:N1722
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length106 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6

Subcellular location

Cytoplasm Ref.8.

Post-translational modification

In wild-type cells, 78% of L42 is monomethylated at both Lys-40 and Lys-55, and 22% are a mixture of species with either residue monomethylated. Ref.10

Miscellaneous

Present with 13600 molecules/cell in log phase SD medium.

There are 2 genes for L42 in yeast.

Sequence similarities

Belongs to the ribosomal protein L44e family.

Mass spectrometry

Molecular mass is 12100.729 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with 2 methylation modifications. Ref.7

Molecular mass is 12100.71 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55. Ref.10

Molecular mass is 12108.0 Da from positions 2 - 106. Determined by ESI. With N6-methyl-Lys-40 and N6-methyl-Lys-55. Ref.10

Sequence caution

The sequence CAA96049.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 10610560S ribosomal protein L42-A
PRO_0000149147

Amino acid modifications

Modified residue401N6-methyllysine; by RKM3 Ref.10
Modified residue551N6-methyllysine; by RKM4 Ref.10

Natural variations

Natural variant561P → Q Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. Ref.1

Experimental info

Sequence conflict40 – 412KR → RK AA sequence Ref.5
Sequence conflict88 – 892Missing AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P0CX27 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 730CA11F2CF7F2B4

FASTA10612,212
        10         20         30         40         50         60 
MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK 

        70         80         90        100 
KAKTTKKVVL RLECVKCKTR AQLTLKRCKH FELGGEKKQK GQALQF 

« Hide

References

« Hide 'large scale' references
[1]"Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts."
Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M.
J. Bacteriol. 174:254-262(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-56.
[2]"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The primary structure of protein 44 from the large subunit of yeast ribosomes."
Itoh T., Wittmann-Liebold B.
FEBS Lett. 96:399-402(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-106.
[6]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[7]"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab."
Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G.
J. Biol. Chem. 283:35561-35568(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-40 AND LYS-55, MASS SPECTROMETRY.
[11]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 2-92, ELECTRON MICROSCOPY.
[12]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
[13]"Crystal structure of the eukaryotic ribosome."
Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
[14]"The structure of the eukaryotic ribosome at 3.0 A resolution."
Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G., Yusupov M.
Science 334:1524-1529(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10578 Genomic DNA. Translation: BAA01435.1.
X92517 Genomic DNA. Translation: CAA63277.1.
Z71438 Genomic DNA. Translation: CAA96049.1. Sequence problems.
BK006947 Genomic DNA. Translation: DAA10387.1.
PIRS63114.
RefSeqNP_012010.1. NM_001179271.1.
NP_014237.2. NM_001183000.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-12-92[»]
1S1Ielectron microscopy11.70Z2-106[»]
3IZSelectron microscopy-r1-106[»]
3O58X-ray4.00f1-106[»]
3O5HX-ray4.00f1-106[»]
3U5EX-ray3.00o1-106[»]
3U5IX-ray3.00o1-106[»]
4B6Aelectron microscopy8.10o1-106[»]
4BYNelectron microscopy4.30o2-106[»]
4BYUelectron microscopy6.60o2-106[»]
ProteinModelPortalP0CX27.
SMRP0CX27. Positions 2-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35667. 28 interactions.
36574. 62 interactions.
MINTMINT-2782780.

Proteomic databases

MaxQBP0CX27.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR141C; YHR141C; YHR141C.
YNL162W; YNL162W; YNL162W.
GeneID855560.
856544.
KEGGsce:YHR141C.
sce:YNL162W.

Organism-specific databases

CYGDYNL162w.
SGDS000005106. RPL42A.

Phylogenomic databases

HOGENOMHOG000224989.
KOK02929.
OrthoDBEOG7MWH8Z.

Enzyme and pathway databases

BioCycYEAST:G3O-33178-MONOMER.

Family and domain databases

Gene3D3.10.450.80. 1 hit.
InterProIPR000552. Ribosomal_L44e.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERPTHR10369. PTHR10369. 1 hit.
PfamPF00935. Ribosomal_L44. 1 hit.
[Graphical view]
ProDomPD002841. Ribosomal_L44e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF57829. SSF57829. 1 hit.
PROSITEPS01172. RIBOSOMAL_L44E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0CX27.
NextBio979652.

Entry information

Entry nameRL44A_YEAST
AccessionPrimary (citable) accession number: P0CX27
Secondary accession number(s): D3DL90, P02405
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references