Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CX27

- RL44A_YEAST

UniProt

P0CX27 - RL44A_YEAST

Protein

60S ribosomal protein L42-A

Gene

RPL42A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. response to antibiotic Source: UniProtKB-KW
    3. response to cycloheximide Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Antibiotic resistance, Cycloheximide resistance

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33178-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L42-A
    Alternative name(s):
    L41
    YL27
    YP44
    Gene namesi
    Name:RPL42A
    Synonyms:RPL41A, SCL41A
    Ordered Locus Names:YNL162W
    ORF Names:N1722
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL162w.
    SGDiS000005106. RPL42A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 10610560S ribosomal protein L42-APRO_0000149147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401N6-methyllysine; by RKM31 Publication
    Modified residuei55 – 551N6-methyllysine; by RKM41 Publication

    Post-translational modificationi

    In wild-type cells, 78% of L42 is monomethylated at both Lys-40 and Lys-55, and 22% are a mixture of species with either residue monomethylated.

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiP0CX27.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi35667. 28 interactions.
    36574. 62 interactions.
    MINTiMINT-2782780.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-12-92[»]
    1S1Ielectron microscopy11.70Z2-106[»]
    1VW8electron microscopy6.10p1-106[»]
    1VWUelectron microscopy6.10p1-106[»]
    3IZSelectron microscopy-r1-106[»]
    3O58X-ray4.00f1-106[»]
    3O5HX-ray4.00f1-106[»]
    3U5EX-ray3.00o1-106[»]
    3U5IX-ray3.00o1-106[»]
    4B6Aelectron microscopy8.10o1-106[»]
    4BYNelectron microscopy4.30o2-106[»]
    4BYUelectron microscopy6.60o2-106[»]
    4CUWelectron microscopy3.70o1-106[»]
    ProteinModelPortaliP0CX27.
    SMRiP0CX27. Positions 2-106.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CX27.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L44e family.Curated

    Phylogenomic databases

    HOGENOMiHOG000224989.
    KOiK02929.
    OrthoDBiEOG7MWH8Z.

    Family and domain databases

    Gene3Di3.10.450.80. 1 hit.
    InterProiIPR000552. Ribosomal_L44e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PANTHERiPTHR10369. PTHR10369. 1 hit.
    PfamiPF00935. Ribosomal_L44. 1 hit.
    [Graphical view]
    ProDomiPD002841. Ribosomal_L44e. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57829. SSF57829. 1 hit.
    PROSITEiPS01172. RIBOSOMAL_L44E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX27-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF    50
    GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCKH FELGGEKKQK 100
    GQALQF 106
    Length:106
    Mass (Da):12,212
    Last modified:June 28, 2011 - v1
    Checksum:i730CA11F2CF7F2B4
    GO

    Sequence cautioni

    The sequence CAA96049.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 412KR → RK AA sequence (PubMed:365584)Curated
    Sequence conflicti88 – 892Missing AA sequence (PubMed:365584)Curated

    Mass spectrometryi

    Molecular mass is 12100.729 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with 2 methylation modifications.1 Publication
    Molecular mass is 12100.71 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55.1 Publication
    Molecular mass is 12108.0 Da from positions 2 - 106. Determined by ESI. With N6-methyl-Lys-40 and N6-methyl-Lys-55.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561P → Q Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10578 Genomic DNA. Translation: BAA01435.1.
    X92517 Genomic DNA. Translation: CAA63277.1.
    Z71438 Genomic DNA. Translation: CAA96049.1. Sequence problems.
    BK006947 Genomic DNA. Translation: DAA10387.1.
    PIRiS63114.
    RefSeqiNP_012010.1. NM_001179271.1.
    NP_014237.2. NM_001183000.1.

    Genome annotation databases

    EnsemblFungiiYHR141C; YHR141C; YHR141C.
    YNL162W; YNL162W; YNL162W.
    GeneIDi855560.
    856544.
    KEGGisce:YHR141C.
    sce:YNL162W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10578 Genomic DNA. Translation: BAA01435.1 .
    X92517 Genomic DNA. Translation: CAA63277.1 .
    Z71438 Genomic DNA. Translation: CAA96049.1 . Sequence problems.
    BK006947 Genomic DNA. Translation: DAA10387.1 .
    PIRi S63114.
    RefSeqi NP_012010.1. NM_001179271.1.
    NP_014237.2. NM_001183000.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - 1 2-92 [» ]
    1S1I electron microscopy 11.70 Z 2-106 [» ]
    1VW8 electron microscopy 6.10 p 1-106 [» ]
    1VWU electron microscopy 6.10 p 1-106 [» ]
    3IZS electron microscopy - r 1-106 [» ]
    3O58 X-ray 4.00 f 1-106 [» ]
    3O5H X-ray 4.00 f 1-106 [» ]
    3U5E X-ray 3.00 o 1-106 [» ]
    3U5I X-ray 3.00 o 1-106 [» ]
    4B6A electron microscopy 8.10 o 1-106 [» ]
    4BYN electron microscopy 4.30 o 2-106 [» ]
    4BYU electron microscopy 6.60 o 2-106 [» ]
    4CUW electron microscopy 3.70 o 1-106 [» ]
    ProteinModelPortali P0CX27.
    SMRi P0CX27. Positions 2-106.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35667. 28 interactions.
    36574. 62 interactions.
    MINTi MINT-2782780.

    Proteomic databases

    MaxQBi P0CX27.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR141C ; YHR141C ; YHR141C .
    YNL162W ; YNL162W ; YNL162W .
    GeneIDi 855560.
    856544.
    KEGGi sce:YHR141C.
    sce:YNL162W.

    Organism-specific databases

    CYGDi YNL162w.
    SGDi S000005106. RPL42A.

    Phylogenomic databases

    HOGENOMi HOG000224989.
    KOi K02929.
    OrthoDBi EOG7MWH8Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33178-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0CX27.
    NextBioi 979652.

    Family and domain databases

    Gene3Di 3.10.450.80. 1 hit.
    InterProi IPR000552. Ribosomal_L44e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    PANTHERi PTHR10369. PTHR10369. 1 hit.
    Pfami PF00935. Ribosomal_L44. 1 hit.
    [Graphical view ]
    ProDomi PD002841. Ribosomal_L44e. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    PROSITEi PS01172. RIBOSOMAL_L44E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts."
      Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M.
      J. Bacteriol. 174:254-262(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-56.
    2. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
      Nasr F., Becam A.-M., Herbert C.J.
      Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The primary structure of protein 44 from the large subunit of yeast ribosomes."
      Itoh T., Wittmann-Liebold B.
      FEBS Lett. 96:399-402(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-106.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab."
      Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G.
      J. Biol. Chem. 283:35561-35568(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-40 AND LYS-55, MASS SPECTROMETRY.
    11. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 2-92, ELECTRON MICROSCOPY.
    12. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    13. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    14. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL44A_YEAST
    AccessioniPrimary (citable) accession number: P0CX27
    Secondary accession number(s): D3DL90, P02405
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13600 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L42 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3