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P0CX25 (RL43A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S ribosomal protein L43-A
Alternative name(s):
L37a
YL35
Gene names
Name:RPL43A
Ordered Locus Names:YPR043W
ORF Names:YP9499.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length92 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4

Subcellular location

Cytoplasm Ref.6.

Miscellaneous

Present with 44600 molecules/cell in log phase SD medium.

There are 2 genes for L43 in yeast.

Sequence similarities

Belongs to the ribosomal protein L37Ae family.

Mass spectrometry

Molecular mass is 9953.311 Da from positions 2 - 92. Determined by ESI. Monoisotopic mass. Ref.5

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionRibonucleoprotein
Ribosomal protein
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcytoplasmic translation

Inferred by curator Ref.5. Source: SGD

   Cellular_componentcytosolic large ribosomal subunit

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural constituent of ribosome

Inferred by curator Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 929160S ribosomal protein L43-A HAMAP-Rule MF_00327
PRO_0000139838

Regions

Zinc finger39 – 6022C4-type HAMAP-Rule MF_00327

Amino acid modifications

Modified residue401Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P0CX25 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: E2057BF56B131730

FASTA9210,091
        10         20         30         40         50         60 
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC 

        70         80         90 
KKTVAGGAYT VSTAAAATVR STIRRLREMV EA 

« Hide

References

« Hide 'large scale' references
[1]"Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI."
Waskiewicz-Staniorowska B., Skala J., Jasinski M., Grenson M., Goffeau A., Ulaszewski S.
Yeast 14:1027-1039(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 46191 / IL125-2B.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[5]"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 10-82, ELECTRON MICROSCOPY.
[10]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
[11]"Crystal structure of the eukaryotic ribosome."
Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
[12]"The structure of the eukaryotic ribosome at 3.0 A resolution."
Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G., Yusupov M.
Science 334:1524-1529(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73616 Genomic DNA. Translation: CAA97993.1.
Z71255 Genomic DNA. Translation: CAA94991.1.
Z49219 Genomic DNA. Translation: CAA89164.1.
BK006949 Genomic DNA. Translation: DAA11468.1.
PIRS54068.
RefSeqNP_012628.3. NM_001181752.3.
NP_015368.1. NM_001184140.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-210-82[»]
1S1Ielectron microscopy11.7092-92[»]
3IZSelectron microscopy-m1-92[»]
3O58X-ray4.00g1-92[»]
3O5HX-ray4.00g1-92[»]
3U5EX-ray3.00p1-92[»]
3U5IX-ray3.00p1-92[»]
4B6Aelectron microscopy8.10p1-92[»]
ProteinModelPortalP0CX25.
SMRP0CX25. Positions 2-92.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33849. 46 interactions.
36220. 76 interactions.
MINTMINT-2781587.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR094W-A; YJR094W-A; YJR094W-A.
YPR043W; YPR043W; YPR043W.
GeneID853557.
856156.
KEGGsce:YJR094W-A.
sce:YPR043W.

Organism-specific databases

CYGDYPR043w.
SGDS000006247. RPL43A.

Phylogenomic databases

KOK02921.
OrthoDBEOG7B5X8P.

Enzyme and pathway databases

BioCycYEAST:G3O-34199-MONOMER.

Family and domain databases

Gene3D2.20.25.30. 1 hit.
HAMAPMF_00327. Ribosomal_L37Ae.
InterProIPR002674. Ribosomal_L37ae.
IPR011331. Ribosomal_L37ae/L37e.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamPF01780. Ribosomal_L37ae. 1 hit.
[Graphical view]
SUPFAMSSF57829. SSF57829. 1 hit.
TIGRFAMsTIGR00280. L37a. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0CX25.
NextBio974302.

Entry information

Entry nameRL43A_YEAST
AccessionPrimary (citable) accession number: P0CX25
Secondary accession number(s): D6VWR3, P49631
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references