60S ribosomal protein L43-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P0CX25 (RL43A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 18. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L43-A

Alternative name(s):
L37a
YL35

Gene names

Name:	RPL43A
Ordered Locus Names:	YPR043W
ORF Names:	YP9499.02

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 44600 molecules/cell in log phase SD medium. There are 2 genes for L43 in yeast.
Sequence similarities	Belongs to the ribosomal protein L37Ae family.
Mass spectrometry	Molecular mass is 9953.311 Da from positions 2 - 92. Determined by ESI. Monoisotopic mass. Ref.5

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.4. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.4. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 92

60S ribosomal protein L43-A

PRO_0000139838

Regions

Zinc finger

39 – 60

C4-type

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P0CX25 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: E2057BF56B131730
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FASTA

10,091

        10         20         30         40         50         60 
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC 

        70         80         90 
KKTVAGGAYT VSTAAAATVR STIRRLREMV EA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI." Waskiewicz-Staniorowska B., Skala J., Jasinski M., Grenson M., Goffeau A., Ulaszewski S. Yeast 14:1027-1039(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR." Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D. Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-40; THR-70 AND SER-81, MASS SPECTROMETRY.
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 10-82, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z73616 Genomic DNA. Translation: CAA97993.1.
Z71255 Genomic DNA. Translation: CAA94991.1.
Z49219 Genomic DNA. Translation: CAA89164.1.
BK006949 Genomic DNA. Translation: DAA11468.1.

PIR

S54068.

RefSeq

NP_012628.3. NM_001181752.3.
NP_015368.1. NM_001184140.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	2	10-82	[»]
1S1I	electron microscopy	11.70	9	2-92	[»]
3IZS	electron microscopy	-	m	1-92	[»]
3O58	X-ray	4.00	g	1-92	[»]
3O5H	X-ray	4.00	g	1-92	[»]
3U5E	X-ray	3.00	p	1-92	[»]
3U5I	X-ray	3.00	p	1-92	[»]
4B6A	electron microscopy	8.10	p	1-92	[»]

ProteinModelPortal

P0CX25.

SMR

P0CX25. Positions 2-92.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJR094W-A; YJR094W-A; YJR094W-A.
YPR043W; YPR043W; YPR043W.

GeneID

853557.
856156.

KEGG

sce:YJR094W-A.
sce:YJR097W.
sce:YPR043W.

Organism-specific databases

CYGD

YPR043w.

SGD

S000006247. RPL43A.

Phylogenomic databases

K02921.

Gene expression databases

ArrayExpress

P0CX25.

GermOnline

YPR043W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.20.25.30. 1 hit.

InterPro

IPR002674. Ribosomal_L37ae.
IPR011331. Ribosomal_L37ae/L37e.
IPR011332. Ribosomal_zn-bd_dom.
[Graphical view]

Pfam

PF01780. Ribosomal_L37ae. 1 hit.
[Graphical view]

SUPFAM

SSF57829. Ribosomal_zn-bd. 1 hit.

TIGRFAMs

TIGR00280. L37a. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX25.

NextBio

974302.

Entry information

Entry name

RL43A_YEAST

Accession

Primary (citable) accession number: P0CX25
Secondary accession number(s): D6VWR3, P49631

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program