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P0CX25

- RL43A_YEAST

UniProt

P0CX25 - RL43A_YEAST

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Protein

60S ribosomal protein L43-A

Gene

RPL43A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri39 – 6022C4-typeAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34199-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L43-A
Alternative name(s):
L37a
YL35
Gene namesi
Name:RPL43A
Ordered Locus Names:YPR043W
ORF Names:YP9499.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR043w.
SGDiS000006247. RPL43A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 929160S ribosomal protein L43-APRO_0000139838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX25.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33849. 46 interactions.
36220. 78 interactions.
MINTiMINT-2781587.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-210-82[»]
1S1Ielectron microscopy11.7092-92[»]
1VW8electron microscopy6.10q1-92[»]
1VWUelectron microscopy6.10q1-92[»]
1VXVelectron microscopy6.20q1-92[»]
1VXYelectron microscopy6.30q1-92[»]
3IZSelectron microscopy-m1-92[»]
3J65electron microscopy8.70k1-92[»]
3O58X-ray4.00g1-92[»]
3O5HX-ray4.00g1-92[»]
3U5EX-ray3.00p1-92[»]
3U5IX-ray3.00p1-92[»]
4B6Aelectron microscopy8.10p1-92[»]
4CUWelectron microscopy3.70p1-92[»]
ProteinModelPortaliP0CX25.
SMRiP0CX25. Positions 2-92.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX25.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L37Ae family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri39 – 6022C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiP0CX25.
KOiK02921.
OrthoDBiEOG7B5X8P.

Family and domain databases

Gene3Di2.20.25.30. 1 hit.
HAMAPiMF_00327. Ribosomal_L37Ae.
InterProiIPR002674. Ribosomal_L37ae.
IPR011331. Ribosomal_L37ae/L37e.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF01780. Ribosomal_L37ae. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.
TIGRFAMsiTIGR00280. L37a. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX25-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG
60 70 80 90
AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREMV EA
Length:92
Mass (Da):10,091
Last modified:June 28, 2011 - v1
Checksum:iE2057BF56B131730
GO

Mass spectrometryi

Molecular mass is 9953.311 Da from positions 2 - 92. Determined by ESI. Monoisotopic mass.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73616 Genomic DNA. Translation: CAA97993.1.
Z71255 Genomic DNA. Translation: CAA94991.1.
Z49219 Genomic DNA. Translation: CAA89164.1.
BK006949 Genomic DNA. Translation: DAA11468.1.
PIRiS54068.
RefSeqiNP_012628.3. NM_001181752.3.
NP_015368.1. NM_001184140.1.

Genome annotation databases

EnsemblFungiiYJR094W-A; YJR094W-A; YJR094W-A.
YPR043W; YPR043W; YPR043W.
GeneIDi853557.
856156.
KEGGisce:YJR094W-A.
sce:YPR043W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73616 Genomic DNA. Translation: CAA97993.1 .
Z71255 Genomic DNA. Translation: CAA94991.1 .
Z49219 Genomic DNA. Translation: CAA89164.1 .
BK006949 Genomic DNA. Translation: DAA11468.1 .
PIRi S54068.
RefSeqi NP_012628.3. NM_001181752.3.
NP_015368.1. NM_001184140.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K5Y model - 2 10-82 [» ]
1S1I electron microscopy 11.70 9 2-92 [» ]
1VW8 electron microscopy 6.10 q 1-92 [» ]
1VWU electron microscopy 6.10 q 1-92 [» ]
1VXV electron microscopy 6.20 q 1-92 [» ]
1VXY electron microscopy 6.30 q 1-92 [» ]
3IZS electron microscopy - m 1-92 [» ]
3J65 electron microscopy 8.70 k 1-92 [» ]
3O58 X-ray 4.00 g 1-92 [» ]
3O5H X-ray 4.00 g 1-92 [» ]
3U5E X-ray 3.00 p 1-92 [» ]
3U5I X-ray 3.00 p 1-92 [» ]
4B6A electron microscopy 8.10 p 1-92 [» ]
4CUW electron microscopy 3.70 p 1-92 [» ]
ProteinModelPortali P0CX25.
SMRi P0CX25. Positions 2-92.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33849. 46 interactions.
36220. 78 interactions.
MINTi MINT-2781587.

Proteomic databases

MaxQBi P0CX25.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR094W-A ; YJR094W-A ; YJR094W-A .
YPR043W ; YPR043W ; YPR043W .
GeneIDi 853557.
856156.
KEGGi sce:YJR094W-A.
sce:YPR043W.

Organism-specific databases

CYGDi YPR043w.
SGDi S000006247. RPL43A.

Phylogenomic databases

InParanoidi P0CX25.
KOi K02921.
OrthoDBi EOG7B5X8P.

Enzyme and pathway databases

BioCyci YEAST:G3O-34199-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

EvolutionaryTracei P0CX25.
NextBioi 974302.

Family and domain databases

Gene3Di 2.20.25.30. 1 hit.
HAMAPi MF_00327. Ribosomal_L37Ae.
InterProi IPR002674. Ribosomal_L37ae.
IPR011331. Ribosomal_L37ae/L37e.
IPR011332. Ribosomal_zn-bd.
[Graphical view ]
Pfami PF01780. Ribosomal_L37ae. 1 hit.
[Graphical view ]
SUPFAMi SSF57829. SSF57829. 1 hit.
TIGRFAMsi TIGR00280. L37a. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI."
    Waskiewicz-Staniorowska B., Skala J., Jasinski M., Grenson M., Goffeau A., Ulaszewski S.
    Yeast 14:1027-1039(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 46191 / IL125-2B.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
    Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
    Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 10-82, ELECTRON MICROSCOPY.
  10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  11. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL43A_YEAST
AccessioniPrimary (citable) accession number: P0CX25
Secondary accession number(s): D6VWR3, P49631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 44600 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L43 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3