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P0CX25

- RL43A_YEAST

UniProt

P0CX25 - RL43A_YEAST

Protein

60S ribosomal protein L43-A

Gene

RPL43A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri39 – 6022C4-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34199-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L43-A
    Alternative name(s):
    L37a
    YL35
    Gene namesi
    Name:RPL43A
    Ordered Locus Names:YPR043W
    ORF Names:YP9499.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR043w.
    SGDiS000006247. RPL43A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 929160S ribosomal protein L43-APRO_0000139838Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP0CX25.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi33849. 46 interactions.
    36220. 77 interactions.
    MINTiMINT-2781587.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-210-82[»]
    1S1Ielectron microscopy11.7092-92[»]
    1VW8electron microscopy6.10q1-92[»]
    1VWUelectron microscopy6.10q1-92[»]
    3IZSelectron microscopy-m1-92[»]
    3J65electron microscopy8.70k1-92[»]
    3O58X-ray4.00g1-92[»]
    3O5HX-ray4.00g1-92[»]
    3U5EX-ray3.00p1-92[»]
    3U5IX-ray3.00p1-92[»]
    4B6Aelectron microscopy8.10p1-92[»]
    4CUWelectron microscopy3.70p1-92[»]
    ProteinModelPortaliP0CX25.
    SMRiP0CX25. Positions 2-92.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CX25.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L37Ae family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri39 – 6022C4-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    KOiK02921.
    OrthoDBiEOG7B5X8P.

    Family and domain databases

    Gene3Di2.20.25.30. 1 hit.
    HAMAPiMF_00327. Ribosomal_L37Ae.
    InterProiIPR002674. Ribosomal_L37ae.
    IPR011331. Ribosomal_L37ae/L37e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PfamiPF01780. Ribosomal_L37ae. 1 hit.
    [Graphical view]
    SUPFAMiSSF57829. SSF57829. 1 hit.
    TIGRFAMsiTIGR00280. L37a. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX25-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG   50
    AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREMV EA 92
    Length:92
    Mass (Da):10,091
    Last modified:June 28, 2011 - v1
    Checksum:iE2057BF56B131730
    GO

    Mass spectrometryi

    Molecular mass is 9953.311 Da from positions 2 - 92. Determined by ESI. Monoisotopic mass.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73616 Genomic DNA. Translation: CAA97993.1.
    Z71255 Genomic DNA. Translation: CAA94991.1.
    Z49219 Genomic DNA. Translation: CAA89164.1.
    BK006949 Genomic DNA. Translation: DAA11468.1.
    PIRiS54068.
    RefSeqiNP_012628.3. NM_001181752.3.
    NP_015368.1. NM_001184140.1.

    Genome annotation databases

    EnsemblFungiiYJR094W-A; YJR094W-A; YJR094W-A.
    YPR043W; YPR043W; YPR043W.
    GeneIDi853557.
    856156.
    KEGGisce:YJR094W-A.
    sce:YPR043W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73616 Genomic DNA. Translation: CAA97993.1 .
    Z71255 Genomic DNA. Translation: CAA94991.1 .
    Z49219 Genomic DNA. Translation: CAA89164.1 .
    BK006949 Genomic DNA. Translation: DAA11468.1 .
    PIRi S54068.
    RefSeqi NP_012628.3. NM_001181752.3.
    NP_015368.1. NM_001184140.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - 2 10-82 [» ]
    1S1I electron microscopy 11.70 9 2-92 [» ]
    1VW8 electron microscopy 6.10 q 1-92 [» ]
    1VWU electron microscopy 6.10 q 1-92 [» ]
    3IZS electron microscopy - m 1-92 [» ]
    3J65 electron microscopy 8.70 k 1-92 [» ]
    3O58 X-ray 4.00 g 1-92 [» ]
    3O5H X-ray 4.00 g 1-92 [» ]
    3U5E X-ray 3.00 p 1-92 [» ]
    3U5I X-ray 3.00 p 1-92 [» ]
    4B6A electron microscopy 8.10 p 1-92 [» ]
    4CUW electron microscopy 3.70 p 1-92 [» ]
    ProteinModelPortali P0CX25.
    SMRi P0CX25. Positions 2-92.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33849. 46 interactions.
    36220. 77 interactions.
    MINTi MINT-2781587.

    Proteomic databases

    MaxQBi P0CX25.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR094W-A ; YJR094W-A ; YJR094W-A .
    YPR043W ; YPR043W ; YPR043W .
    GeneIDi 853557.
    856156.
    KEGGi sce:YJR094W-A.
    sce:YPR043W.

    Organism-specific databases

    CYGDi YPR043w.
    SGDi S000006247. RPL43A.

    Phylogenomic databases

    KOi K02921.
    OrthoDBi EOG7B5X8P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34199-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P0CX25.
    NextBioi 974302.

    Family and domain databases

    Gene3Di 2.20.25.30. 1 hit.
    HAMAPi MF_00327. Ribosomal_L37Ae.
    InterProi IPR002674. Ribosomal_L37ae.
    IPR011331. Ribosomal_L37ae/L37e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    Pfami PF01780. Ribosomal_L37ae. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    TIGRFAMsi TIGR00280. L37a. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI."
      Waskiewicz-Staniorowska B., Skala J., Jasinski M., Grenson M., Goffeau A., Ulaszewski S.
      Yeast 14:1027-1039(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 46191 / IL125-2B.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    5. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 10-82, ELECTRON MICROSCOPY.
    10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    11. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL43A_YEAST
    AccessioniPrimary (citable) accession number: P0CX25
    Secondary accession number(s): D6VWR3, P49631
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 44600 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L43 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3