P0CW81 (EBRA_BACAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug resistance protein EbrA

Gene names

Name:

ebrA

Organism

Bacillus atrophaeus

Taxonomic identifier

1452 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons. Ref.1
Subunit structure	The efflux pump is composed of EbrA and EbrB Probable. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the small multidrug resistance (SMR) protein family. EbrA/EbrB (TC 2.A.7.1.6) subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Multidrug resistance protein EbrA		PRO_0000408956
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	57 – 77	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P0CW81 [UniParc]. Last modified May 31, 2011. Version 1. Checksum: 93B4760294D87676	FASTA	105	11,392
	10 20 30 40 50 60 MLVGYIFLTI AICSESIGAA MLKVSDGFKK WKPSALVVIA YSLAFYMLSL TLNHIPLSLS 70 80 90 100 YATWSGVGTV LTAVIGVKWF KEELNAKGLI GILLLISGVV LLNWQ

References

[1]

"A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis." Masaoka Y., Ueno Y., Morita Y., Kuroda T., Mizushima T., Tsuchiya T. J. Bacteriol. 182:2307-2310(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: ATCC 9372 / DSM 675 / NBRC 13721 / NCIMB 8058 / NRS 1221A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB029306 Genomic DNA. Translation: BAA88575.1.
3D structure databases
ProteinModelPortal	P0CW81.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000390. Small_multidrug_res. [Graphical view]
Pfam	PF00893. Multi_Drug_Res. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBRA_BACAT

Accession

Primary (citable) accession number: P0CW81
Secondary accession number(s): O31792, Q9R9I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	May 31, 2011
Last modified:	April 3, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program