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Protein

Secreted protein BARF1

Gene

BARF1

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays diverse functions in immunomodulation and oncogenicity, maybe by acting as a functional receptor for human CSF1. May inhibit interferon secretion from mononuclear cells. Exhibits oncogenic activity in vitro (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: IntAct
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted protein BARF1
Alternative name(s):
33 kDa early protein
p33
Gene namesi
Name:BARF1
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007641 Componenti: Genome

Subcellular locationi

  • Secreted

  • Note: Massively secreted in the serum of EBV-induced nasophyryngeal carcinoma patients.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 221201Secreted protein BARF1PRO_0000408390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...); by host
Disulfide bondi146 ↔ 201PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated on serine and threonine by host.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homohexamer. Interacts with human CSF1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2620133,EBI-2620133

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-60066N.
IntActiP0CW72. 4 interactions.

Structurei

Secondary structure

1
221
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 243Combined sources
Beta strandi29 – 346Combined sources
Beta strandi44 – 529Combined sources
Turni53 – 553Combined sources
Beta strandi56 – 649Combined sources
Beta strandi67 – 704Combined sources
Helixi72 – 743Combined sources
Beta strandi78 – 836Combined sources
Beta strandi86 – 938Combined sources
Helixi96 – 983Combined sources
Beta strandi100 – 1089Combined sources
Beta strandi111 – 13323Combined sources
Beta strandi142 – 15211Combined sources
Beta strandi155 – 1584Combined sources
Beta strandi183 – 1919Combined sources
Beta strandi199 – 2079Combined sources
Beta strandi209 – 2168Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FA8X-ray2.20A/B/D19-221[»]
ProteinModelPortaliP0CW72.
SMRiP0CW72. Positions 21-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 120100Ig-like 1Add
BLAST
Domaini124 – 22097Ig-like 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CW72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFIAQLLL LASCVAAGQA VTAFLGERVT LTSYWRRVSL GPEIEVSWFK
60 70 80 90 100
LGPGEEQVLI GRMHHDVIFI EWPFRGFFDI HRSANTFFLV VTAANISHDG
110 120 130 140 150
NYLCRMKLGE TEVTKQEHLS VVKPLTLSVH SERSQFPDFS VLTVTCTVNA
160 170 180 190 200
FPHPHVQWLM PEGVEPAPTA ANGGVMKEKD GSLSVAVDLS LPKPWHLPVT
210 220
CVGKNDKEEA HGVYVSGYLS Q
Length:221
Mass (Da):24,471
Last modified:May 3, 2011 - v1
Checksum:iCA5A24D1EA28758E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41158.1.
RefSeqiYP_401719.1. NC_007605.1.

Genome annotation databases

GeneIDi3783772.
KEGGivg:3783772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41158.1.
RefSeqiYP_401719.1. NC_007605.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FA8X-ray2.20A/B/D19-221[»]
ProteinModelPortaliP0CW72.
SMRiP0CW72. Positions 21-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60066N.
IntActiP0CW72. 4 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783772.
KEGGivg:3783772.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBARF1_EBVG
AccessioniPrimary (citable) accession number: P0CW72
Secondary accession number(s): Q777A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: October 14, 2015
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.