ID   MALX4_YEAST             Reviewed;         589 AA.
AC   P0CW41; D6VVX6; P40439;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   13-SEP-2023, entry version 74.
DE   RecName: Full=Oligo-1,6-glucosidase IMA4;
DE            EC=3.2.1.10;
DE   AltName: Full=Alpha-glucosidase;
DE   AltName: Full=Flocculent-specific protein 2;
DE   AltName: Full=Isomaltase 4;
GN   Name=IMA4; Synonyms=FSP2; OrderedLocusNames=YJL221C;
GN   ORFNames=HRE589, J0218;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7725802; DOI=10.1002/yea.320101216;
RA   Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.;
RT   "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere
RT   of yeast chromosome X.";
RL   Yeast 10:1657-1662(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=20562106; DOI=10.1074/jbc.m110.145946;
RA   Teste M.A., Francois J.M., Parrou J.L.;
RT   "Characterization of a new multigene family encoding isomaltases in the
RT   yeast Saccharomyces cerevisiae, the IMA family.";
RL   J. Biol. Chem. 285:26815-26824(2010).
CC   -!- FUNCTION: Alpha-glucosidase with broad substrate specificity for alpha-
CC       1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization,
CC       but overexpression allows the IMA1 null mutant to grow on isomaltose.
CC       {ECO:0000269|PubMed:20562106}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some
CC         oligosaccharides produced from starch and glycogen by alpha-amylase,
CC         and in isomaltose.; EC=3.2.1.10;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z34098; CAA83990.1; -; Genomic_DNA.
DR   EMBL; Z49496; CAA89517.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08592.1; -; Genomic_DNA.
DR   PIR; S50355; S50355.
DR   RefSeq; NP_012096.1; NM_001179518.1.
DR   RefSeq; NP_012314.1; NM_001181654.1.
DR   AlphaFoldDB; P0CW41; -.
DR   SMR; P0CW41; -.
DR   BioGRID; 33561; 14.
DR   BioGRID; 34824; 5.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblFungi; YIL172C_mRNA; YIL172C; YIL172C.
DR   EnsemblFungi; YJL221C_mRNA; YJL221C; YJL221C.
DR   GeneID; 853235; -.
DR   GeneID; 854635; -.
DR   KEGG; sce:YIL172C; -.
DR   KEGG; sce:YJL221C; -.
DR   AGR; SGD:S000003757; -.
DR   SGD; S000003757; IMA4.
DR   VEuPathDB; FungiDB:YIL172C; -.
DR   VEuPathDB; FungiDB:YJL221C; -.
DR   GeneTree; ENSGT00940000176291; -.
DR   HOGENOM; CLU_006462_1_1_1; -.
DR   InParanoid; P0CW41; -.
DR   OrthoDB; 3680211at2759; -.
DR   BioCyc; YEAST:YJL221C-MONOMER; -.
DR   Reactome; R-SCE-352230; Amino acid transport across the plasma membrane.
DR   PRO; PR:P0CW41; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P0CW41; Protein.
DR   GO; GO:0004556; F:alpha-amylase activity; IBA:GO_Central.
DR   GO; GO:0033934; F:glucan 1,4-alpha-maltotriohydrolase activity; IBA:GO_Central.
DR   GO; GO:0032450; F:maltose alpha-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0004574; F:oligo-1,6-glucosidase activity; IMP:SGD.
DR   GO; GO:0004575; F:sucrose alpha-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0046352; P:disaccharide catabolic process; IGI:SGD.
DR   GO; GO:0000025; P:maltose catabolic process; IBA:GO_Central.
DR   GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR   CDD; cd11333; AmyAc_SI_OligoGlu_DGase; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 2.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045857; O16G_dom_2.
DR   PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR   PANTHER; PTHR10357:SF179; NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Maltose metabolism; Reference proteome.
FT   CHAIN           1..589
FT                   /note="Oligo-1,6-glucosidase IMA4"
FT                   /id="PRO_0000408197"
FT   ACT_SITE        215
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        277
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   SITE            352
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   589 AA;  68699 MW;  0323A2677148EC29 CRC64;
     MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK ELGTDAIWIS
     PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL GMKFITDLVI NHCSSEHEWF
     KESRSSKTNP KRDWFFWRPP KGYDAEGKPI PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS
     TQPDLNWENE DCRKAIYESA VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQL
     SDPFTMNGPR IHEFHQEMNK FIRNRVKDGR EIMTVGEMRH ATDETKRLYT SASRHELSEL
     FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN HDQPRSITRF
     GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK NWPIEKYEDV EVRNNYDAIK
     EEHGENSKEM KRFLEAIALI SRDHARTPMQ WSREEPNAGF SGPNAKPWFY LNESFREGIN
     AEDESKDPNS VLNFWKEALR FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA
     LNFSSDSIDF TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE
//