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P0CW41

- MALX4_YEAST

UniProt

P0CW41 - MALX4_YEAST

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Protein

Oligo-1,6-glucosidase IMA4

Gene

IMA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization, but overexpression allows the IMA1 null mutant to grow on isomaltose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151NucleophileBy similarity
Active sitei277 – 2771Proton donorBy similarity
Sitei352 – 3521Transition state stabilizerBy similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: SGD

GO - Biological processi

  1. disaccharide catabolic process Source: SGD
  2. maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YJL221C-MONOMER.
ReactomeiREACT_243973. Amino acid transport across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA4 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Flocculent-specific protein 2
Isomaltase 4
Gene namesi
Name:IMA4
Synonyms:FSP2
Ordered Locus Names:YJL221C
ORF Names:HRE589, J0218
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

SGDiS000003757. IMA4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 589588Oligo-1,6-glucosidase IMA4PRO_0000408197Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi34824. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliP0CW41.
SMRiP0CW41. Positions 4-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063127.
HOGENOMiHOG000220641.
InParanoidiP0CW41.
KOiK01187.
OrthoDBiEOG7M3J7X.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CW41-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGTDAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQL SDPFTMNGPR
260 270 280 290 300
IHEFHQEMNK FIRNRVKDGR EIMTVGEMRH ATDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK
410 420 430 440 450
NWPIEKYEDV EVRNNYDAIK EEHGENSKEM KRFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPNAKPWFY LNESFREGIN AEDESKDPNS VLNFWKEALR
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA LNFSSDSIDF
560 570 580
TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,699
Last modified:May 3, 2011 - v1
Checksum:i0323A2677148EC29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83990.1.
Z49496 Genomic DNA. Translation: CAA89517.1.
BK006943 Genomic DNA. Translation: DAA08592.1.
PIRiS50355.
RefSeqiNP_012096.1. NM_001179518.1.
NP_012314.1. NM_001181654.1.

Genome annotation databases

EnsemblFungiiYIL172C; YIL172C; YIL172C.
YJL221C; YJL221C; YJL221C.
GeneIDi853235.
854635.
KEGGisce:YIL172C.
sce:YJL221C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83990.1 .
Z49496 Genomic DNA. Translation: CAA89517.1 .
BK006943 Genomic DNA. Translation: DAA08592.1 .
PIRi S50355.
RefSeqi NP_012096.1. NM_001179518.1.
NP_012314.1. NM_001181654.1.

3D structure databases

ProteinModelPortali P0CW41.
SMRi P0CW41. Positions 4-589.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34824. 5 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL172C ; YIL172C ; YIL172C .
YJL221C ; YJL221C ; YJL221C .
GeneIDi 853235.
854635.
KEGGi sce:YIL172C.
sce:YJL221C.

Organism-specific databases

SGDi S000003757. IMA4.

Phylogenomic databases

GeneTreei ENSGT00530000063127.
HOGENOMi HOG000220641.
InParanoidi P0CW41.
KOi K01187.
OrthoDBi EOG7M3J7X.

Enzyme and pathway databases

BioCyci YEAST:YJL221C-MONOMER.
Reactomei REACT_243973. Amino acid transport across the plasma membrane.

Miscellaneous databases

NextBioi 973455.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
    Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family."
    Teste M.A., Francois J.M., Parrou J.L.
    J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMALX4_YEAST
AccessioniPrimary (citable) accession number: P0CW41
Secondary accession number(s): D6VVX6, P40439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: November 26, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3