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Protein

Oligo-1,6-glucosidase IMA4

Gene

IMA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization, but overexpression allows the IMA1 null mutant to grow on isomaltose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215NucleophileBy similarity1
Active sitei277Proton donorBy similarity1
Sitei352Transition state stabilizerBy similarity1

GO - Molecular functioni

  • oligo-1,6-glucosidase activity Source: SGD

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processMaltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YJL221C-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA4 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Flocculent-specific protein 2
Isomaltase 4
Gene namesi
Name:IMA4
Synonyms:FSP2
Ordered Locus Names:YJL221C
ORF Names:HRE589, J0218
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003757. IMA4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004081971 – 589Oligo-1,6-glucosidase IMA4Add BLAST589

Proteomic databases

PRIDEiP0CW41.

Interactioni

Protein-protein interaction databases

BioGridi33561. 14 interactors.
34824. 5 interactors.

Structurei

3D structure databases

ProteinModelPortaliP0CW41.
SMRiP0CW41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000140965.
HOGENOMiHOG000220641.
InParanoidiP0CW41.
KOiK01182.
OrthoDBiEOG092C12JX.

Family and domain databases

InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CW41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGTDAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQL SDPFTMNGPR
260 270 280 290 300
IHEFHQEMNK FIRNRVKDGR EIMTVGEMRH ATDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK
410 420 430 440 450
NWPIEKYEDV EVRNNYDAIK EEHGENSKEM KRFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPNAKPWFY LNESFREGIN AEDESKDPNS VLNFWKEALR
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA LNFSSDSIDF
560 570 580
TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,699
Last modified:May 3, 2011 - v1
Checksum:i0323A2677148EC29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83990.1.
Z49496 Genomic DNA. Translation: CAA89517.1.
BK006943 Genomic DNA. Translation: DAA08592.1.
PIRiS50355.
RefSeqiNP_012096.1. NM_001179518.1.
NP_012314.1. NM_001181654.1.

Genome annotation databases

EnsemblFungiiYIL172C; YIL172C; YIL172C.
YJL221C; YJL221C; YJL221C.
GeneIDi853235.
854635.
KEGGisce:YIL172C.
sce:YJL221C.

Similar proteinsi

Entry informationi

Entry nameiMALX4_YEAST
AccessioniPrimary (citable) accession number: P0CW41
Secondary accession number(s): D6VVX6, P40439
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: November 22, 2017
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names