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P0CW41

- MALX4_YEAST

UniProt

P0CW41 - MALX4_YEAST

Protein

Oligo-1,6-glucosidase IMA4

Gene

IMA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (03 May 2011)
      Previous versions | rss
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    Functioni

    Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization, but overexpression allows the IMA1 null mutant to grow on isomaltose.1 Publication

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei215 – 2151NucleophileBy similarity
    Active sitei277 – 2771Proton donorBy similarity
    Sitei352 – 3521Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. oligo-1,6-glucosidase activity Source: SGD

    GO - Biological processi

    1. disaccharide catabolic process Source: SGD
    2. maltose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Maltose metabolism

    Enzyme and pathway databases

    BioCyciYEAST:YJL221C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oligo-1,6-glucosidase IMA4 (EC:3.2.1.10)
    Alternative name(s):
    Alpha-glucosidase
    Flocculent-specific protein 2
    Isomaltase 4
    Gene namesi
    Name:IMA4
    Synonyms:FSP2
    Ordered Locus Names:YJL221C
    ORF Names:HRE589, J0218
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    SGDiS000003757. IMA4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 589588Oligo-1,6-glucosidase IMA4PRO_0000408197Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi34824. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CW41.
    SMRiP0CW41. Positions 4-589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00530000063127.
    HOGENOMiHOG000220641.
    KOiK01187.
    OrthoDBiEOG7M3J7X.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CW41-1 [UniParc]FASTAAdd to Basket

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    MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK    50
    ELGTDAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL 100
    GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI 150
    PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA 200
    VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQL SDPFTMNGPR 250
    IHEFHQEMNK FIRNRVKDGR EIMTVGEMRH ATDETKRLYT SASRHELSEL 300
    FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN 350
    HDQPRSITRF GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK 400
    NWPIEKYEDV EVRNNYDAIK EEHGENSKEM KRFLEAIALI SRDHARTPMQ 450
    WSREEPNAGF SGPNAKPWFY LNESFREGIN AEDESKDPNS VLNFWKEALR 500
    FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA LNFSSDSIDF 550
    TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE 589
    Length:589
    Mass (Da):68,699
    Last modified:May 3, 2011 - v1
    Checksum:i0323A2677148EC29
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34098 Genomic DNA. Translation: CAA83990.1.
    Z49496 Genomic DNA. Translation: CAA89517.1.
    BK006943 Genomic DNA. Translation: DAA08592.1.
    PIRiS50355.
    RefSeqiNP_012096.1. NM_001179518.1.
    NP_012314.1. NM_001181654.1.

    Genome annotation databases

    EnsemblFungiiYIL172C; YIL172C; YIL172C.
    YJL221C; YJL221C; YJL221C.
    GeneIDi853235.
    854635.
    KEGGisce:YIL172C.
    sce:YJL221C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34098 Genomic DNA. Translation: CAA83990.1 .
    Z49496 Genomic DNA. Translation: CAA89517.1 .
    BK006943 Genomic DNA. Translation: DAA08592.1 .
    PIRi S50355.
    RefSeqi NP_012096.1. NM_001179518.1.
    NP_012314.1. NM_001181654.1.

    3D structure databases

    ProteinModelPortali P0CW41.
    SMRi P0CW41. Positions 4-589.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34824. 5 interactions.

    Chemistry

    BindingDBi P0CW41.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL172C ; YIL172C ; YIL172C .
    YJL221C ; YJL221C ; YJL221C .
    GeneIDi 853235.
    854635.
    KEGGi sce:YIL172C.
    sce:YJL221C.

    Organism-specific databases

    SGDi S000003757. IMA4.

    Phylogenomic databases

    GeneTreei ENSGT00530000063127.
    HOGENOMi HOG000220641.
    KOi K01187.
    OrthoDBi EOG7M3J7X.

    Enzyme and pathway databases

    BioCyci YEAST:YJL221C-MONOMER.

    Miscellaneous databases

    NextBioi 973455.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
      Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family."
      Teste M.A., Francois J.M., Parrou J.L.
      J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMALX4_YEAST
    AccessioniPrimary (citable) accession number: P0CW41
    Secondary accession number(s): D6VVX6, P40439
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3