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Protein

Elongation factor 1-alpha-A

Gene

tef101

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPBy similarity
Nucleotide bindingi91 – 955GTPBy similarity
Nucleotide bindingi153 – 1564GTPBy similarity

GO - Molecular functioni

  • calmodulin binding Source: PomBase
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational elongation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156842. Eukaryotic Translation Elongation.
R-SPO-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha-A
Short name:
EF-1-alpha-A
Gene namesi
Name:tef101
Synonyms:ef1a-a, tef1a, tef1e
ORF Names:SPCC794.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

PomBaseiSPCC794.09c. tef101.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation elongation factor 1 complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Elongation factor 1-alpha-APRO_0000090968Add
BLAST

Proteomic databases

PRIDEiP50522.

Interactioni

GO - Molecular functioni

  • calmodulin binding Source: PomBase

Protein-protein interaction databases

MINTiMINT-1214081.

Structurei

3D structure databases

ProteinModelPortaliP0CT53.
SMRiP0CT53. Positions 2-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 240236tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1By similarity
Regioni70 – 745G2By similarity
Regioni91 – 944G3By similarity
Regioni153 – 1564G4By similarity
Regioni192 – 1943G5By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiP50522.
KOiK03231.
OMAiWSEARFG.
PhylomeDBiP0CT53.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CT53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEKGHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEATELG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDIALWKF ETPKYNVTVI DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIIGGGT GEFEAGISKD GQTREHALLA YTLGVKQLIV
160 170 180 190 200
AVNKMDTTGW SQARFEEIVK ETSNFIKKVG FNPKTVPFVP VSGFQGDNMI
210 220 230 240 250
EPTTNMPWYQ GWQKETKAGV VKGKTLLEAI DSIEPPARPT DKPLRLPLQD
260 270 280 290 300
VYKIGGIGTV PVGRVETGVI KPGMIVTFAP AGVTTEVKSV EMHHESLDAG
310 320 330 340 350
LPGDNVGFNV KNVSVKDIRR GNVCGDSKND PPMGCASFTA QVIILNHPGQ
360 370 380 390 400
ISAGYSPVLD CHTAHIACKF AELIEKIDRR SGKKIEESPK FVKSGDACIA
410 420 430 440 450
KMVPSKPMCV EAFTDYAPLG RFAVRDMRQT VAVGVIKAVE KVAPGAAKVT
460
KAAVKAGAKK
Length:460
Mass (Da):49,660
Last modified:June 24, 2015 - v1
Checksum:i82EA58185D514294
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471T → F in AAB63859 (Ref. 5) By similarity
Sequence conflicti47 – 471T → F in AAB63865 (Ref. 5) By similarity
Sequence conflicti57 – 571A → S in AAB63859 (Ref. 5) By similarity
Sequence conflicti57 – 571A → S in AAB63865 (Ref. 5) By similarity
Sequence conflicti113 – 1131V → I in BAA19867 (PubMed:9501991).By similarity
Sequence conflicti139 – 1391L → R in BAA11569 (PubMed:9099890).By similarity
Sequence conflicti139 – 1391L → V in BAA19867 (PubMed:9501991).By similarity
Sequence conflicti140 – 1401A → G in AAA85129 (PubMed:10593605).By similarity
Sequence conflicti422 – 4221F → FG in AAA85129 (PubMed:10593605).By similarity

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82571 mRNA. Translation: BAA11569.1.
U42189 mRNA. Translation: AAA85129.1.
D89112 mRNA. Translation: BAA19867.1.
CU329672 Genomic DNA. Translation: CAA19136.1.
U97367 mRNA. Translation: AAB63859.1.
U97373 mRNA. Translation: AAB63865.1.
PIRiB41453.
T41617.
T42089.
T43267.
T43704.
RefSeqiNP_587757.1. NM_001022750.2.

Genome annotation databases

EnsemblFungiiSPCC794.09c.1; SPCC794.09c.1:pep; SPCC794.09c.
GeneIDi2539035.
KEGGispo:SPCC794.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82571 mRNA. Translation: BAA11569.1.
U42189 mRNA. Translation: AAA85129.1.
D89112 mRNA. Translation: BAA19867.1.
CU329672 Genomic DNA. Translation: CAA19136.1.
U97367 mRNA. Translation: AAB63859.1.
U97373 mRNA. Translation: AAB63865.1.
PIRiB41453.
T41617.
T42089.
T43267.
T43704.
RefSeqiNP_587757.1. NM_001022750.2.

3D structure databases

ProteinModelPortaliP0CT53.
SMRiP0CT53. Positions 2-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1214081.

Proteomic databases

PRIDEiP50522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC794.09c.1; SPCC794.09c.1:pep; SPCC794.09c.
GeneIDi2539035.
KEGGispo:SPCC794.09c.

Organism-specific databases

PomBaseiSPCC794.09c. tef101.

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiP50522.
KOiK03231.
OMAiWSEARFG.
PhylomeDBiP0CT53.

Enzyme and pathway databases

ReactomeiR-SPO-156842. Eukaryotic Translation Elongation.
R-SPO-3371511. HSF1 activation.

Miscellaneous databases

PROiP0CT53.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A1_SCHPO
AccessioniPrimary (citable) accession number: P0CT53
Secondary accession number(s): O14372
, O14441, O59818, P50522, P78764, Q10117, Q10119, Q10158, Q7M4U9, Q7Z8V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: June 24, 2015
Last modified: September 7, 2016
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.