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Protein

Elongation factor 1-alpha-A

Gene

tef101

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPBy similarity8
Nucleotide bindingi91 – 95GTPBy similarity5
Nucleotide bindingi153 – 156GTPBy similarity4

GO - Molecular functioni

  • calmodulin binding Source: PomBase
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational elongation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156842. Eukaryotic Translation Elongation.
R-SPO-3371511. HSF1 activation.
R-SPO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha-A
Short name:
EF-1-alpha-A
Gene namesi
Name:tef101
Synonyms:ef1a-a, tef1a, tef1e
ORF Names:SPCC794.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC794.09c.
PomBaseiSPCC794.09c. tef101.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation elongation factor 1 complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000909681 – 460Elongation factor 1-alpha-AAdd BLAST460

Interactioni

GO - Molecular functioni

  • calmodulin binding Source: PomBase

Protein-protein interaction databases

MINTiMINT-1214081.

Structurei

3D structure databases

ProteinModelPortaliP0CT53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 240tr-type GAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1By similarity8
Regioni70 – 74G2By similarity5
Regioni91 – 94G3By similarity4
Regioni153 – 156G4By similarity4
Regioni192 – 194G5By similarity3

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiP50522.
KOiK03231.
OMAiWSEARFG.
PhylomeDBiP0CT53.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CT53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEKGHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEATELG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDIALWKF ETPKYNVTVI DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIIGGGT GEFEAGISKD GQTREHALLA YTLGVKQLIV
160 170 180 190 200
AVNKMDTTGW SQARFEEIVK ETSNFIKKVG FNPKTVPFVP VSGFQGDNMI
210 220 230 240 250
EPTTNMPWYQ GWQKETKAGV VKGKTLLEAI DSIEPPARPT DKPLRLPLQD
260 270 280 290 300
VYKIGGIGTV PVGRVETGVI KPGMIVTFAP AGVTTEVKSV EMHHESLDAG
310 320 330 340 350
LPGDNVGFNV KNVSVKDIRR GNVCGDSKND PPMGCASFTA QVIILNHPGQ
360 370 380 390 400
ISAGYSPVLD CHTAHIACKF AELIEKIDRR SGKKIEESPK FVKSGDACIA
410 420 430 440 450
KMVPSKPMCV EAFTDYAPLG RFAVRDMRQT VAVGVIKAVE KVAPGAAKVT
460
KAAVKAGAKK
Length:460
Mass (Da):49,660
Last modified:June 24, 2015 - v1
Checksum:i82EA58185D514294
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47T → F in AAB63859 (Ref. 5) By similarity1
Sequence conflicti47T → F in AAB63865 (Ref. 5) By similarity1
Sequence conflicti57A → S in AAB63859 (Ref. 5) By similarity1
Sequence conflicti57A → S in AAB63865 (Ref. 5) By similarity1
Sequence conflicti113V → I in BAA19867 (PubMed:9501991).By similarity1
Sequence conflicti139L → R in BAA11569 (PubMed:9099890).By similarity1
Sequence conflicti139L → V in BAA19867 (PubMed:9501991).By similarity1
Sequence conflicti140A → G in AAA85129 (PubMed:10593605).By similarity1
Sequence conflicti422F → FG in AAA85129 (PubMed:10593605).By similarity1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82571 mRNA. Translation: BAA11569.1.
U42189 mRNA. Translation: AAA85129.1.
D89112 mRNA. Translation: BAA19867.1.
CU329672 Genomic DNA. Translation: CAA19136.1.
U97367 mRNA. Translation: AAB63859.1.
U97373 mRNA. Translation: AAB63865.1.
PIRiB41453.
T41617.
T42089.
T43267.
T43704.
RefSeqiNP_587757.1. NM_001022750.2.

Genome annotation databases

EnsemblFungiiSPCC794.09c.1; SPCC794.09c.1:pep; SPCC794.09c.
GeneIDi2539035.
KEGGispo:SPCC794.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82571 mRNA. Translation: BAA11569.1.
U42189 mRNA. Translation: AAA85129.1.
D89112 mRNA. Translation: BAA19867.1.
CU329672 Genomic DNA. Translation: CAA19136.1.
U97367 mRNA. Translation: AAB63859.1.
U97373 mRNA. Translation: AAB63865.1.
PIRiB41453.
T41617.
T42089.
T43267.
T43704.
RefSeqiNP_587757.1. NM_001022750.2.

3D structure databases

ProteinModelPortaliP0CT53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1214081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC794.09c.1; SPCC794.09c.1:pep; SPCC794.09c.
GeneIDi2539035.
KEGGispo:SPCC794.09c.

Organism-specific databases

EuPathDBiFungiDB:SPCC794.09c.
PomBaseiSPCC794.09c. tef101.

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiP50522.
KOiK03231.
OMAiWSEARFG.
PhylomeDBiP0CT53.

Enzyme and pathway databases

ReactomeiR-SPO-156842. Eukaryotic Translation Elongation.
R-SPO-3371511. HSF1 activation.
R-SPO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP0CT53.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A1_SCHPO
AccessioniPrimary (citable) accession number: P0CT53
Secondary accession number(s): O14372
, O14441, O59818, P50522, P78764, Q10117, Q10119, Q10158, Q7M4U9, Q7Z8V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: June 24, 2015
Last modified: November 30, 2016
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.