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Protein

Heat shock protein SSC1, mitochondrial

Gene

SSC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. Required for the import of the processed fratxin homolog YFH1 into the mitochondrion.3 Publications
Acts as a non-catalytic component of endonuclease SceI (Endo.SceI), which cleaves specifically at multiple sites on mitochondrial DNA and produces double-stranded breaks. SSC1 confers broader sequence specificity, greater stability, and higher activity on the catalytic subunit (By similarity).By similarity

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • enzyme regulator activity Source: SGD

GO - Biological processi

  • positive regulation of endodeoxyribonuclease activity Source: SGD
  • protein import into mitochondrial matrix Source: SGD
  • protein refolding Source: SGD
  • protein unfolding Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31680-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein SSC1, mitochondrial
Alternative name(s):
Endonuclease SceI 75 kDa subunit
Short name:
Endo.SceI 75 kDa subunit
mtHSP70
Gene namesi
Name:SSC1
Synonyms:ENS1
Ordered Locus Names:YJR045C
ORF Names:J1639
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR045C.
SGDiS000003806. SSC1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: SGD
  • mitochondrial nucleoid Source: SGD
  • nucleus Source: UniProtKB-SubCell
  • presequence translocase-associated import motor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi442P → S: No interaction with TIM44. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23Mitochondrion1 PublicationAdd BLAST23
ChainiPRO_000001355324 – 654Heat shock protein SSC1, mitochondrialAdd BLAST631

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei330PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CS90.
PRIDEiP0CS90.

PTM databases

iPTMnetiP0CS90.

Interactioni

Subunit structurei

Component of endonuclease SceI (Endo.SceI), which is a heterodimer of ENS2 and SSC1 (By similarity). Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. In the complex, SSC1 interacts directly with PAM18 and TIM44. Interacts with NAP1.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ENS2P122942EBI-8637,EBI-6490From a different organism.
TIM44Q018523EBI-8637,EBI-9141

Protein-protein interaction databases

BioGridi33797. 114 interactors.
IntActiP0CS90. 50 interactors.
MINTiMINT-665405.

Structurei

3D structure databases

ProteinModelPortaliP0CS90.
SMRiP0CS90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi634 – 646Asn-richAdd BLAST13

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00800000124275.
InParanoidiP0CS90.
KOiK04043.
OMAiENQWDEE.
OrthoDBiEOG092C1VPN.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK. 1 hit.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CS90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAAKNILNR SSLSSSFRIA TRLQSTKVQG SVIGIDLGTT NSAVAIMEGK
60 70 80 90 100
VPKIIENAEG SRTTPSVVAF TKEGERLVGI PAKRQAVVNP ENTLFATKRL
110 120 130 140 150
IGRRFEDAEV QRDIKQVPYK IVKHSNGDAW VEARGQTYSP AQIGGFVLNK
160 170 180 190 200
MKETAEAYLG KPVKNAVVTV PAYFNDSQRQ ATKDAGQIVG LNVLRVVNEP
210 220 230 240 250
TAAALAYGLE KSDSKVVAVF DLGGGTFDIS ILDIDNGVFE VKSTNGDTHL
260 270 280 290 300
GGEDFDIYLL REIVSRFKTE TGIDLENDRM AIQRIREAAE KAKIELSSTV
310 320 330 340 350
STEINLPFIT ADASGPKHIN MKFSRAQFET LTAPLVKRTV DPVKKALKDA
360 370 380 390 400
GLSTSDISEV LLVGGMSRMP KVVETVKSLF GKDPSKAVNP DEAVAIGAAV
410 420 430 440 450
QGAVLSGEVT DVLLLDVTPL SLGIETLGGV FTRLIPRNTT IPTKKSQIFS
460 470 480 490 500
TAAAGQTSVE IRVFQGEREL VRDNKLIGNF TLAGIPPAPK GVPQIEVTFD
510 520 530 540 550
IDADGIINVS ARDKATNKDS SITVAGSSGL SENEIEQMVN DAEKFKSQDE
560 570 580 590 600
ARKQAIETAN KADQLANDTE NSLKEFEGKV DKAEAQKVRD QITSLKELVA
610 620 630 640 650
RVQGGEEVNA EELKTKTEEL QTSSMKLFEQ LYKNDSNNNN NNNGNNAESG

ETKQ
Length:654
Mass (Da):70,628
Last modified:June 13, 2012 - v1
Checksum:i1E5672E76FCE24AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27229 Genomic DNA. Translation: AAA63792.1.
L36344 Genomic DNA. Translation: AAA88747.1.
Z49545 Genomic DNA. Translation: CAA89573.1.
BK006943 Genomic DNA. Translation: DAA08832.1.
PIRiA32493. HHBYS1.
RefSeqiNP_012579.1. NM_001181703.1.

Genome annotation databases

EnsemblFungiiYJR045C; YJR045C; YJR045C.
GeneIDi853503.
KEGGisce:YJR045C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27229 Genomic DNA. Translation: AAA63792.1.
L36344 Genomic DNA. Translation: AAA88747.1.
Z49545 Genomic DNA. Translation: CAA89573.1.
BK006943 Genomic DNA. Translation: DAA08832.1.
PIRiA32493. HHBYS1.
RefSeqiNP_012579.1. NM_001181703.1.

3D structure databases

ProteinModelPortaliP0CS90.
SMRiP0CS90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33797. 114 interactors.
IntActiP0CS90. 50 interactors.
MINTiMINT-665405.

PTM databases

iPTMnetiP0CS90.

Proteomic databases

MaxQBiP0CS90.
PRIDEiP0CS90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR045C; YJR045C; YJR045C.
GeneIDi853503.
KEGGisce:YJR045C.

Organism-specific databases

EuPathDBiFungiDB:YJR045C.
SGDiS000003806. SSC1.

Phylogenomic databases

GeneTreeiENSGT00800000124275.
InParanoidiP0CS90.
KOiK04043.
OMAiENQWDEE.
OrthoDBiEOG092C1VPN.

Enzyme and pathway databases

BioCyciYEAST:G3O-31680-MONOMER.

Miscellaneous databases

PROiP0CS90.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK. 1 hit.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSP77_YEAST
AccessioniPrimary (citable) accession number: P0CS90
Secondary accession number(s): D6VWL6, P12398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 13, 2012
Last modified: November 30, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

S.cerevisiae displays strain polymorphism with regard to Endo.SceI endouclease activity. This is due to the mitochondrion-encoded, catalytic subunit ENS2, which exhibits strain polymorphism. It can be either present as continuous ORF in the mitochondrial genome (e.g. strain IAM 4274), present but disrupted by the insertion of GC clusters (e.g. strain D273-10B/A), or completely absent in the mitochondrial genome (e.g. strain S288c). Sequences for the 2 subunits ENS2 (AC P12294) and SSC1 (AC P0CS91) for an active Endo.SceI endonuclease can be found in strain IAM 4274.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.