Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei178S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei181UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Ordered Locus Names:CNBB5420
OrganismiCryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Taxonomic identifieri283643 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000001435 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:CNBB5420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004103171 – 286tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST286

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi283643.XP_777016.1

Structurei

3D structure databases

ProteinModelPortaliP0CS81
SMRiP0CS81
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni114 – 115S-adenosyl-L-methionine bindingUniRule annotation2
Regioni158 – 159S-adenosyl-L-methionine bindingUniRule annotation2
Regioni256 – 258S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
HOGENOMiHOG000260965
KOiK03439
OrthoDBiEOG092C0GMH

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

P0CS81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAGPSTASP GVSVSPAPAL TAGEVQLLKV PQKRFYRQRA HANVFIDHEL
60 70 80 90 100
DYPKRPELMD WSTHYPAYFS QPNEDGTITQ GEKKVEWADV GCGFGGLLMA
110 120 130 140 150
LAPLFPEKLM LGMEIRTSVT KYVTDRIAAT RQAQSLLPAD SVDTKPGGYQ
160 170 180 190 200
NVSVIKANSM KHMPNFFAKG QLEKIFFLFP DPHFKNRKHK ARIITPALLA
210 220 230 240 250
EYAYVLRPGG ILYTVTDVKD LHEWMAHHLH AHPLFEYIPT ETLSDDPILE
260 270 280
AARTATEEGQ KVERNKGDKW VACFRRKEDP KEEDED
Length:286
Mass (Da):32,263
Last modified:June 28, 2011 - v1
Checksum:iED46ACEFCD44A732
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEY01000011 Genomic DNA Translation: EAL22369.1
RefSeqiXP_777016.1, XM_771923.1

Genome annotation databases

EnsemblFungiiEAL22369; EAL22369; CNBB5420
GeneIDi4934638
KEGGicnb:CNBB5420

Similar proteinsi

Entry informationi

Entry nameiTRMB_CRYNB
AccessioniPrimary (citable) accession number: P0CS81
Secondary accession number(s): Q55X18, Q5KMW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: March 28, 2018
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health