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P0CS80 (TRMB_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
Ordered Locus Names:CNB00250
OrganismCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome]
Taxonomic identifier214684 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370594

Regions

Region114 – 1152S-adenosyl-L-methionine binding By similarity
Region158 – 1592S-adenosyl-L-methionine binding By similarity
Region256 – 2583S-adenosyl-L-methionine binding By similarity

Sites

Active site1811 By similarity
Binding site911S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1781S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P0CS80 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: ED46ACEFCD44A732

FASTA28632,263
        10         20         30         40         50         60 
MEAGPSTASP GVSVSPAPAL TAGEVQLLKV PQKRFYRQRA HANVFIDHEL DYPKRPELMD 

        70         80         90        100        110        120 
WSTHYPAYFS QPNEDGTITQ GEKKVEWADV GCGFGGLLMA LAPLFPEKLM LGMEIRTSVT 

       130        140        150        160        170        180 
KYVTDRIAAT RQAQSLLPAD SVDTKPGGYQ NVSVIKANSM KHMPNFFAKG QLEKIFFLFP 

       190        200        210        220        230        240 
DPHFKNRKHK ARIITPALLA EYAYVLRPGG ILYTVTDVKD LHEWMAHHLH AHPLFEYIPT 

       250        260        270        280 
ETLSDDPILE AARTATEEGQ KVERNKGDKW VACFRRKEDP KEEDED 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017342 Genomic DNA. Translation: AAW41459.1.
RefSeqXP_568766.1. XM_568766.1.

3D structure databases

ProteinModelPortalP0CS80.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAW41459; AAW41459; CNB00250.
GeneID3256005.
KEGGcne:CNB00250.

Phylogenomic databases

KOK03439.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CRYNJ
AccessionPrimary (citable) accession number: P0CS80
Secondary accession number(s): Q55X18, Q5KMW7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways