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Protein

Serine/threonine-protein kinase SSN3

Gene

SSN3

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141ATPPROSITE-ProRule annotation
Active sitei216 – 2161Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: EnsemblFungi
  2. negative regulation of filamentous growth Source: EnsemblFungi
  3. nuclear-transcribed mRNA catabolic process, non-stop decay Source: EnsemblFungi
  4. phosphorylation of RNA polymerase II C-terminal domain Source: EnsemblFungi
  5. positive regulation of G2/M transition of mitotic cell cycle Source: EnsemblFungi
  6. positive regulation of transcription from RNA polymerase II promoter by galactose Source: EnsemblFungi
  7. protein destabilization Source: EnsemblFungi
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SSN3 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cyclin-dependent kinase 8
Gene namesi
Name:SSN3
Synonyms:CDK8
Ordered Locus Names:CNM00930
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000002149 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiFungiDB:CNM00930.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Serine/threonine-protein kinase SSN3PRO_0000312942Add
BLAST

Interactioni

Subunit structurei

Component of the SRB8-11 complex, a regulatory module of the Mediator complex.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0CS76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 396365Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP0CS76.
KOiK02208.
OMAiTHEDNGD.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CS76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIPGGGTI MDPMHLYRAR RDKERRGVLK TYKILGFISS GTYGRVYKAV
60 70 80 90 100
LLPPPKTASA KSALPSSTRA ALSLPKDKLP SPSFTEDSDP LNNPEMCMRP
110 120 130 140 150
GDRPAKRGDV FAIKKFKPDK EGDVLTYAGI SQSGAREIML NRELHHRNLV
160 170 180 190 200
SLREVILEDK SIYMVFEYAE HDFLQIIHYH SQTARASIPP STLRRLLHQL
210 220 230 240 250
LCGVHFLHSN FVLHRDLKPA NILVTSQGVV KIGDLGLARL WHKPLAQQGL
260 270 280 290 300
YGGDKVVVTI WYRAPELILG AKHYTAAVDI WAVGCIYAEL LSLRPIFKGD
310 320 330 340 350
EAKMDGKKSL PFQRDQMGKI CEVLGPVKPE QWPGIVHMPE YRTYQATGPY
360 370 380 390 400
PHSNPLAPWY HARSNSSEGY DILVKMFEWD PARRITARDA LRHPWFQEEG
410 420 430 440 450
GVDTKSVFEG SSITYPTRRV THEDNGDAKM GSLPQSMAGG RLPSSSNFRP
460
ASGNIVQPAA RKKARI
Length:466
Mass (Da):52,124
Last modified:June 28, 2011 - v1
Checksum:i8CA4D04E5334C9C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017353 Genomic DNA. Translation: AAW46899.1.
RefSeqiXP_568416.1. XM_568416.1.
UniGeneiFne.1167.

Genome annotation databases

EnsemblFungiiAAW46899; AAW46899; CNM00930.
GeneIDi3255225.
KEGGicne:CNM00930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017353 Genomic DNA. Translation: AAW46899.1.
RefSeqiXP_568416.1. XM_568416.1.
UniGeneiFne.1167.

3D structure databases

ProteinModelPortaliP0CS76.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAW46899; AAW46899; CNM00930.
GeneIDi3255225.
KEGGicne:CNM00930.

Organism-specific databases

EuPathDBiFungiDB:CNM00930.

Phylogenomic databases

InParanoidiP0CS76.
KOiK02208.
OMAiTHEDNGD.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
    Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
    , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
    Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JEC21 / ATCC MYA-565.

Entry informationi

Entry nameiSSN3_CRYNJ
AccessioniPrimary (citable) accession number: P0CS76
Secondary accession number(s): Q55ID8, Q5K7X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 29, 2015
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.