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Protein

Thymidylate synthase

Gene

TMP1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40dUMPBy similarity1
Active sitei187NucleophileBy similarity1
Binding sitei2195,10-methylenetetrahydrofolateBy similarity1
Binding sitei227dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 168dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi216 – 219dUMPBy similarity4
Nucleotide bindingi257 – 259dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TMP1
Ordered Locus Names:CNBJ2230
OrganismiCryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Taxonomic identifieri283643 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000001435 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiFungiDB:CNBJ2230.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4665.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004103191 – 317Thymidylate synthaseAdd BLAST317

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi283643.XP_772947.1.

Chemistry databases

BindingDBiP0CS13.

Structurei

3D structure databases

ProteinModelPortaliP0CS13.
SMRiP0CS13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
KOiK00560.
OrthoDBiEOG092C3QQ6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CS13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATIDDQEK NQRSNPDHEE YQYLDLIRRI INVGEVRPDR TGTGTVALFA
60 70 80 90 100
PPSFRFSLAD NTLPLLTTKR VFLRGVIAEL LWFVSGCTDA KMLSSQGVGI
110 120 130 140 150
WDGNGSKEFL EKVGLGHRRE GDLGPVYGFQ WRHFGAEYTD ADGDYKGKGV
160 170 180 190 200
DQLQRVIDTI KNNPTDRRII LSAWNPKDLP LMALPPCHMF CQFFVSLPPA
210 220 230 240 250
DSPGSKPKLS CLMYQRSCDL GLGVPFNIAS YALLTHMIAL ITDTEPHEFI
260 270 280 290 300
LQMGDAHVYR DHVEPLKTQL EREPRDFPKL KWARSKEEIG DIDGFKVEDF
310
VVEGYKPWGK IDMKMSA
Length:317
Mass (Da):35,866
Last modified:June 28, 2011 - v1
Checksum:iE6322588CE1B50B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12256 Genomic DNA. Translation: AAC48931.1.
AAEY01000050 Genomic DNA. Translation: EAL18300.1.
RefSeqiXP_772947.1. XM_767854.1.

Genome annotation databases

EnsemblFungiiEAL18300; EAL18300; CNBJ2230.
GeneIDi4938566.
KEGGicnb:CNBJ2230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12256 Genomic DNA. Translation: AAC48931.1.
AAEY01000050 Genomic DNA. Translation: EAL18300.1.
RefSeqiXP_772947.1. XM_767854.1.

3D structure databases

ProteinModelPortaliP0CS13.
SMRiP0CS13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi283643.XP_772947.1.

Chemistry databases

BindingDBiP0CS13.
ChEMBLiCHEMBL4665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAL18300; EAL18300; CNBJ2230.
GeneIDi4938566.
KEGGicnb:CNBJ2230.

Organism-specific databases

EuPathDBiFungiDB:CNBJ2230.

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
KOiK00560.
OrthoDBiEOG092C3QQ6.

Enzyme and pathway databases

UniPathwayiUPA00575.

Miscellaneous databases

PROiP0CS13.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_CRYNB
AccessioniPrimary (citable) accession number: P0CS13
Secondary accession number(s): P45351, Q55KW0, Q5KAL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.