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Protein

Thymidylate synthase

Gene

TMP1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathway: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TMP1
Ordered Locus Names:CNJ01230
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000002149 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiFungiDB:CNJ01230.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Thymidylate synthasePRO_0000140910Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi214684.XP_567501.1.

Structurei

Secondary structure

1
317
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 3314Combined sources
Beta strandi35 – 373Combined sources
Beta strandi45 – 506Combined sources
Beta strandi54 – 574Combined sources
Helixi73 – 8513Combined sources
Helixi91 – 944Combined sources
Turni95 – 973Combined sources
Helixi102 – 1054Combined sources
Helixi107 – 1126Combined sources
Helixi127 – 1337Combined sources
Helixi152 – 16211Combined sources
Beta strandi170 – 1723Combined sources
Turni176 – 1783Combined sources
Helixi179 – 1813Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi187 – 19610Combined sources
Beta strandi208 – 21912Combined sources
Turni220 – 2223Combined sources
Helixi223 – 24220Combined sources
Beta strandi245 – 25915Combined sources
Helixi260 – 2623Combined sources
Helixi263 – 2708Combined sources
Beta strandi279 – 2846Combined sources
Helixi286 – 2894Combined sources
Helixi297 – 2993Combined sources
Beta strandi300 – 3034Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AAZX-ray2.08A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-317[»]
ProteinModelPortaliP0CS12.
SMRiP0CS12. Positions 19-316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CS12.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

InParanoidiP0CS12.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG7X6M9G.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CS12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATIDDQEK NQRSNPDHEE YQYLDLIRRI INVGEVRPDR TGTGTVALFA
60 70 80 90 100
PPSFRFSLAD NTLPLLTTKR VFLRGVIAEL LWFVSGCTDA KMLSSQGVGI
110 120 130 140 150
WDGNGSKEFL EKVGLGHRRE GDLGPVYGFQ WRHFGAEYTD ADGDYKGKGV
160 170 180 190 200
DQLQRVIDTI KNNPTDRRII LSAWNPKDLP LMALPPCHMF CQFFVSLPPA
210 220 230 240 250
DSPGSKPKLS CLMYQRSCDL GLGVPFNIAS YALLTHMIAL ITDTEPHEFI
260 270 280 290 300
LQMGDAHVYR DHVEPLKTQL EREPRDFPKL KWARSKEEIG DIDGFKVEDF
310
VVEGYKPWGK IDMKMSA
Length:317
Mass (Da):35,866
Last modified:June 28, 2011 - v1
Checksum:iE6322588CE1B50B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017350 Genomic DNA. Translation: AAW45984.1.
RefSeqiXP_567501.1. XM_567501.1.

Genome annotation databases

EnsemblFungiiAAW45984; AAW45984; CNJ01230.
GeneIDi3254191.
KEGGicne:CNJ01230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017350 Genomic DNA. Translation: AAW45984.1.
RefSeqiXP_567501.1. XM_567501.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AAZX-ray2.08A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-317[»]
ProteinModelPortaliP0CS12.
SMRiP0CS12. Positions 19-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi214684.XP_567501.1.

Chemistry

BindingDBiP0CS12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAW45984; AAW45984; CNJ01230.
GeneIDi3254191.
KEGGicne:CNJ01230.

Organism-specific databases

EuPathDBiFungiDB:CNJ01230.

Phylogenomic databases

InParanoidiP0CS12.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG7X6M9G.

Enzyme and pathway databases

UniPathwayiUPA00575.

Miscellaneous databases

EvolutionaryTraceiP0CS12.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
    Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
    , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
    Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JEC21 / ATCC MYA-565.

Entry informationi

Entry nameiTYSY_CRYNJ
AccessioniPrimary (citable) accession number: P0CS12
Secondary accession number(s): P45351, Q55KW0, Q5KAL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 24, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.