P0CS12 (TYSY_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	TMP1
Ordered Locus Names:	CNJ01230

Organism

Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome]

Taxonomic identifier

214684 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex › Cryptococcus neoformans › Cryptococcus neoformans var. neoformans

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 317

317

Thymidylate synthase

PRO_0000140910

Sites

Active site

187

1

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

317

Helix Strand Turn

Details...

Helix

20 – 33

14

Beta strand

35 – 37

3

Beta strand

45 – 50

6

Beta strand

54 – 57

4

Helix

73 – 85

13

Helix

91 – 94

4

Turn

95 – 97

3

Helix

102 – 105

4

Helix

107 – 112

6

Helix

127 – 133

7

Helix

152 – 162

11

Beta strand

170 – 172

3

Turn

176 – 178

3

Helix

179 – 181

3

Beta strand

182 – 184

3

Beta strand

187 – 196

10

Beta strand

208 – 219

12

Turn

220 – 222

3

Helix

223 – 242

20

Beta strand

245 – 259

15

Helix

260 – 262

3

Helix

263 – 270

8

Beta strand

279 – 284

6

Helix

286 – 289

4

Helix

297 – 299

3

Beta strand

300 – 303

4

Sequences

	Sequence		Length	Mass (Da)
	P0CS12 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: E6322588CE1B50B2	FASTA	317	35,866
	10 20 30 40 50 60 MTATIDDQEK NQRSNPDHEE YQYLDLIRRI INVGEVRPDR TGTGTVALFA PPSFRFSLAD 70 80 90 100 110 120 NTLPLLTTKR VFLRGVIAEL LWFVSGCTDA KMLSSQGVGI WDGNGSKEFL EKVGLGHRRE 130 140 150 160 170 180 GDLGPVYGFQ WRHFGAEYTD ADGDYKGKGV DQLQRVIDTI KNNPTDRRII LSAWNPKDLP 190 200 210 220 230 240 LMALPPCHMF CQFFVSLPPA DSPGSKPKLS CLMYQRSCDL GLGVPFNIAS YALLTHMIAL 250 260 270 280 290 300 ITDTEPHEFI LQMGDAHVYR DHVEPLKTQL EREPRDFPKL KWARSKEEIG DIDGFKVEDF 310 VVEGYKPWGK IDMKMSA

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JEC21 / ATCC MYA-565.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE017350 Genomic DNA. Translation: AAW45984.1.

RefSeq

XP_567501.1. XM_567501.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AAZ	X-ray	2.08	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P	1-317	[»]

ProteinModelPortal

P0CS12.

SMR

P0CS12. Positions 19-316.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3254191.

KEGG

cne:CNJ01230.

Phylogenomic databases

KO

K00560.

Enzyme and pathway databases

UniPathway

UPA00575.

Family and domain databases

Gene3D

3.30.572.10. 1 hit.

InterPro

IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]

Pfam

PF00303. Thymidylat_synt. 1 hit.
[Graphical view]

PRINTS

PR00108. THYMDSNTHASE.

SUPFAM

SSF55831. Thymidylat_synth_C. 1 hit.

TIGRFAMs

TIGR03284. thym_sym. 1 hit.

PROSITE

PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P0CS12.

EvolutionaryTrace

P0CS12.

Entry information

Entry name

TYSY_CRYNJ

Accession

Primary (citable) accession number: P0CS12
Secondary accession number(s): P45351, Q55KW0, Q5KAL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	March 6, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program