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Protein

Thymidylate synthase

Gene

TMP1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40dUMPBy similarity1
Active sitei187NucleophileBy similarity1
Binding sitei2195,10-methylenetetrahydrofolateBy similarity1
Binding sitei227dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 168dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi216 – 219dUMPBy similarity4
Nucleotide bindingi257 – 259dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TMP1
Ordered Locus Names:CNJ01230
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000002149 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiFungiDB:CNJ01230.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409101 – 317Thymidylate synthaseAdd BLAST317

Proteomic databases

PaxDbiP0CS12.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi214684.XP_567501.1.

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 33Combined sources14
Beta strandi35 – 37Combined sources3
Beta strandi45 – 50Combined sources6
Beta strandi54 – 57Combined sources4
Helixi73 – 85Combined sources13
Helixi91 – 94Combined sources4
Turni95 – 97Combined sources3
Helixi102 – 105Combined sources4
Helixi107 – 112Combined sources6
Helixi127 – 133Combined sources7
Helixi152 – 162Combined sources11
Beta strandi170 – 172Combined sources3
Turni176 – 178Combined sources3
Helixi179 – 181Combined sources3
Beta strandi182 – 184Combined sources3
Beta strandi187 – 196Combined sources10
Beta strandi208 – 219Combined sources12
Turni220 – 222Combined sources3
Helixi223 – 242Combined sources20
Beta strandi245 – 259Combined sources15
Helixi260 – 262Combined sources3
Helixi263 – 270Combined sources8
Beta strandi279 – 284Combined sources6
Helixi286 – 289Combined sources4
Helixi297 – 299Combined sources3
Beta strandi300 – 303Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AAZX-ray2.08A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-317[»]
ProteinModelPortaliP0CS12.
SMRiP0CS12.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CS12.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
InParanoidiP0CS12.
KOiK00560.
OMAiPHKTIAM.
OrthoDBiEOG092C3QQ6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CS12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATIDDQEK NQRSNPDHEE YQYLDLIRRI INVGEVRPDR TGTGTVALFA
60 70 80 90 100
PPSFRFSLAD NTLPLLTTKR VFLRGVIAEL LWFVSGCTDA KMLSSQGVGI
110 120 130 140 150
WDGNGSKEFL EKVGLGHRRE GDLGPVYGFQ WRHFGAEYTD ADGDYKGKGV
160 170 180 190 200
DQLQRVIDTI KNNPTDRRII LSAWNPKDLP LMALPPCHMF CQFFVSLPPA
210 220 230 240 250
DSPGSKPKLS CLMYQRSCDL GLGVPFNIAS YALLTHMIAL ITDTEPHEFI
260 270 280 290 300
LQMGDAHVYR DHVEPLKTQL EREPRDFPKL KWARSKEEIG DIDGFKVEDF
310
VVEGYKPWGK IDMKMSA
Length:317
Mass (Da):35,866
Last modified:June 28, 2011 - v1
Checksum:iE6322588CE1B50B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017350 Genomic DNA. Translation: AAW45984.1.
RefSeqiXP_567501.1. XM_567501.1.

Genome annotation databases

EnsemblFungiiAAW45984; AAW45984; CNJ01230.
GeneIDi3254191.
KEGGicne:CNJ01230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017350 Genomic DNA. Translation: AAW45984.1.
RefSeqiXP_567501.1. XM_567501.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AAZX-ray2.08A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-317[»]
ProteinModelPortaliP0CS12.
SMRiP0CS12.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi214684.XP_567501.1.

Proteomic databases

PaxDbiP0CS12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAW45984; AAW45984; CNJ01230.
GeneIDi3254191.
KEGGicne:CNJ01230.

Organism-specific databases

EuPathDBiFungiDB:CNJ01230.

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
InParanoidiP0CS12.
KOiK00560.
OMAiPHKTIAM.
OrthoDBiEOG092C3QQ6.

Enzyme and pathway databases

UniPathwayiUPA00575.

Miscellaneous databases

EvolutionaryTraceiP0CS12.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_CRYNJ
AccessioniPrimary (citable) accession number: P0CS12
Secondary accession number(s): P45351, Q55KW0, Q5KAL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.