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Protein

Mitogen-activated protein kinase CPK1

Gene

CPK1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Responds to activation by environmental stress by phosphorylating downstream targets.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPPROSITE-ProRule annotation1
Active sitei140Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 30ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase CPK1 (EC:2.7.11.24)
Alternative name(s):
Stress-activated protein kinase CPK1
Gene namesi
Name:CPK1
Ordered Locus Names:CNE00520
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000002149 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:CNE00520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863241 – 366Mitogen-activated protein kinase CPK1Add BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphothreonineBy similarity1
Modified residuei183PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0CP66

Interactioni

Protein-protein interaction databases

STRINGi214684.XP_570760.1

Structurei

3D structure databases

ProteinModelPortaliP0CP66
SMRiP0CP66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 302Protein kinasePROSITE-ProRule annotationAdd BLAST286

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 183TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
InParanoidiP0CP66
KOiK04371
OMAiMEKCLTF
OrthoDBiEOG092C2FL8

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P0CP66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDQSQINF NVPSTYKLEE IVGEGAYGLV VAGTHLPSGT QVAIKRITPF
60 70 80 90 100
DHTMFCQRTL REIKLLRHFH HENIISILDL IQPESYEVFN EVYLVQELME
110 120 130 140 150
TDLHRVIRSQ ELSDDHCQYF VYQTLRGLKA LHSADVLHRD LKPSNLLLNA
160 170 180 190 200
NCDLKICDFG LARSSAKPPP GTSDGGQGFM TEYVATRWYR APEVMLSFQE
210 220 230 240 250
YTKAIDLWSV GCILAEMING KPLFPGRDYH HQLSLILQVL GTPTMDDFNE
260 270 280 290 300
ITSQRSKDYL RALEFTRRQD FSAICPKAKP AAVDLLKQTL TFSPSKRITV
310 320 330 340 350
EEALMHSYVE AYHDPHDEPN AEPLKPGFFD FEFHQEKLSR DQWKRMIYDE
360
VQDPVPTILS QWTESH
Length:366
Mass (Da):42,120
Last modified:June 28, 2011 - v1
Checksum:i1BA353D5D67CF84E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF414186 Genomic DNA Translation: AAN03694.1
AE017345 Genomic DNA Translation: AAW43453.1
RefSeqiXP_570760.1, XM_570760.1
UniGeneiFne.1104

Genome annotation databases

EnsemblFungiiAAW43453; AAW43453; CNE00520
GeneIDi3257550
KEGGicne:CNE00520

Similar proteinsi

Entry informationi

Entry nameiCPK1_CRYNJ
AccessioniPrimary (citable) accession number: P0CP66
Secondary accession number(s): Q55SZ2, Q5KHC4, Q8NK05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: March 28, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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