P0CP47 (PABP_CRYNB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyadenylate-binding protein, cytoplasmic and nuclear
Short name=PABP
Short name=Poly(A)-binding protein

Alternative name(s):
Polyadenylate tail-binding protein

Gene names

Name:	PAB1
Ordered Locus Names:	CNBH1100

Organism

Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

283643 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex › Cryptococcus neoformans › Cryptococcus neoformans var. neoformans

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the polyadenylate-binding protein type-1 family. Contains 1 PABC domain. Contains 4 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Translation regulation Transport mRNA processing mRNA transport
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	Polyadenylate-binding protein, cytoplasmic and nuclear		PRO_0000410181
Regions
	Domain	46 – 124	79	RRM 1
	Domain	134 – 211	78	RRM 2
	Domain	227 – 304	78	RRM 3
	Domain	330 – 407	78	RRM 4
	Domain	569 – 646	78	PABC
	Compositional bias	443 – 567	125	Pro-rich
	Compositional bias	662 – 665	4	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	P0CP47 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: B41F74AD0F3F6427	FASTA	673	72,708
	10 20 30 40 50 60 MSAETATSPA PAAETPVAPA PATQTTPAEG APTPAAAAPG GNTVSASLYV GELDPSVTEA 70 80 90 100 110 120 MLFEIFNMIG PVASIRVCRD AVTRRSLGYA YVNYLNAADG ERALEHLNYS LIKGQSCRIM 130 140 150 160 170 180 WSQRDPALRK TGQGNIFIKN LDQSIDNKAL HDTFAAFGDI LSCKVGTDEN GKSRGFAFVH 190 200 210 220 230 240 YSTGEAADAA IKAVNGMLLN DKKVYVGHHV GKKERLSKVE ELRAQFTNVY IKNVDLEVTD 250 260 270 280 290 300 AEFEDLVKPF GPTISVALSR DEKGVSKGFG FVNYENHESA RKAVDELNEK EVNGKKLYAG 310 320 330 340 350 360 RAQTKSEREA ELKKSHEEKR LENEAKSAGV NLYVKNLDDE WDDDRLRAEF EAFGTITSSK 370 380 390 400 410 420 VMRDDSGVSR GFGFVCYSSP DEATKAVSEM NGKMIGTKPL YVALAQRKDV RRQALESQIA 430 440 450 460 470 480 QRAQQRMQYG AGFPGMQGYM GQPMYGYPPM PGYGQPMPGM PPVRGPMMGY PGAPQNMMQS 490 500 510 520 530 540 RPRFNPNGQP LPGGVPAYGM PPQVPYPGAP GYPVRPGGAR IPAAPNANGP RNGGPSPVGA 550 560 570 580 590 600 PQGLPAGSIP RGGQMPARPH EQAAPAPQAG RLDAQSLARA APAEQKQMLG EALYPLIHET 610 620 630 640 650 660 QPELAGKITG MLLEMDNAEL LHLVESQPAL QEKVDEALRV LAEWGKDEKP AADEGAEEPK 670 KEEEETKEEE KKE

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAEY01000041 Genomic DNA. Translation: EAL19418.1.
RefSeq	XP_774065.1. XM_768972.1.
3D structure databases
ProteinModelPortal	P0CP47.
SMR	P0CP47. Positions 46-215, 330-417, 566-641.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4937630.
KEGG	cnb:CNBH1100.
Phylogenomic databases
KO	K13126.
Family and domain databases
Gene3D	1.10.1900.10. 1 hit. 3.30.70.330. 4 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR006515. PABP_1234. IPR002004. PABP_HYD. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00658. PABP. 1 hit. PF00076. RRM_1. 4 hits. [Graphical view]
SMART	SM00517. PolyA. 1 hit. SM00360. RRM. 4 hits. [Graphical view]
SUPFAM	SSF63570. PABP_HYD. 1 hit.
TIGRFAMs	TIGR01628. PABP-1234. 1 hit.
PROSITE	PS51309. PABC. 1 hit. PS50102. RRM. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PABP_CRYNB

Accession

Primary (citable) accession number: P0CP47
Secondary accession number(s): Q55NH7, Q5KBW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	March 6, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program