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Protein

Lysophospholipase NTE1

Gene

NTE1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by organophosphorus esters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1349NucleophilePROSITE-ProRule annotation1
Active sitei1467Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi788 – 907cNMP 1Add BLAST120
Nucleotide bindingi951 – 1070cNMP 2Add BLAST120

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase NTE1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:NTE1
Ordered Locus Names:CNBD2140
OrganismiCryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Taxonomic identifieri283643 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000001435 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:CNBD2140.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicBy similarityAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 59LumenalBy similarityAdd BLAST26
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 1621CytoplasmicBy similarityAdd BLAST1541

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004101761 – 1621Lysophospholipase NTE1Add BLAST1621

Interactioni

Protein-protein interaction databases

STRINGi283643.XP_776167.1.

Structurei

3D structure databases

ProteinModelPortaliP0CP37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1316 – 1480PNPLAPROSITE-ProRule annotationAdd BLAST165

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1320 – 1325GXGXXGPROSITE-ProRule annotation6
Motifi1347 – 1351GXSXGPROSITE-ProRule annotation5
Motifi1467 – 1469DGA/GPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
HOGENOMiHOG000048680.
KOiK14676.
OrthoDBiEOG092C0BAH.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 5 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CP37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIPTPPDA NGNPLIALAV AVIYAILYVL QGVKYGVSLL TIGIPSCIVR
60 70 80 90 100
MLQYSLTISL GFPHLLALFA GALLALFFLI RYRYLTRYAQ LKESALPPPS
110 120 130 140 150
PPALASRLLP LDGDGLGLPD SRSQTSSFHN YLDDFLSAIR IFGYLEKPVF
160 170 180 190 200
HELSRHLQTR RLAAGDTLEI GGGEFWCVVE GKVQVFAPDA SSQGTPTPSS
210 220 230 240 250
DTNSPTRPSF NGYHLLNEVS TGGTLSSLFS ILSLFTEDIK LSWKSSADDE
260 270 280 290 300
GEEEQIFEGA PEQSSAKLRV RRANSDVSQL GPDSIGVRAM DPTPLPESID
310 320 330 340 350
SHGDSSVPQR RRERSSSIDA AGETVREREG IFASASLPIS STEPPSPRRS
360 370 380 390 400
QSLRSSPRLN SATNLLSSQS EHLRSSVPRK AGIEIGSKAL KGTIARATED
410 420 430 440 450
TTLAVIPAAA FRKLTRKFPK ASGTIVQVVL ERFSRVTFMT AHKYLGLTRE
460 470 480 490 500
ILQTESSLNL LVTHPLPRSF YTGGGMQALR ARFQPEALAK ESVHYDSLKS
510 520 530 540 550
SPNARVSSKD YFNYVPASPT VKAPSLPAMT PKPLSPIIHK SSLGQTATTT
560 570 580 590 600
VKNEPLNGGS SPLDETRDKV PSFGLSTAAA TNPDASFRHA SPFIRRTSAM
610 620 630 640 650
RQQVAAGDLA MSVHNLPDES GQAYYRPTAI TPGLSKMDTW QRRYSSSWNL
660 670 680 690 700
NDSPHTDGQP VDPQRDDESL LNESFDLKEA VLNSIAKSIG LYQEAESNSD
710 720 730 740 750
MIARSSMAPS VSALSTPNSP MFPPNAGTPL QGSTRSRPPH FGNVLDLINA
760 770 780 790 800
SSQNEGVIGG MLREAAFNSR PDDEASSISM SLHDSQGGAS GVDRKIMKDL
810 820 830 840 850
ERHVEILFFK KGSVLVKEGE RSPGMYYVID GFLETSLPFR STSSNQENPN
860 870 880 890 900
STPGSKHRQS SFGSSNERPF KTALGLDTSK GKELDDGSKK DEALFTVKPG
910 920 930 940 950
GIAGYLSSLC CTDSYVDITA KTDCFVGFLP HHTLERIIER RPIVLLTLAK
960 970 980 990 1000
RLLSLLSPLV LHIDAALDWQ QLNAGQVLYE KGDKSTDFYI VINGRLRAFT
1010 1020 1030 1040 1050
EKNDNMHVLR EYGQNDSIGE LDVITAVDRS ETVHAIRDSE LVRIPAALFD
1060 1070 1080 1090 1100
AISIKHPETT VQFMRLIAGR VRRALGDEMN GRVPGLPTTD MNLKTVCVLG
1110 1120 1130 1140 1150
STRNVPVTQF AGKLKNALEE IGASTSYLDQ GIVMRHLGRH AFARIGKLKV
1160 1170 1180 1190 1200
AGWLADQEQH YRTVLYVADS PPASQWTLTC IRQADLVLVV SMGDDPSLGE
1210 1220 1230 1240 1250
YEKLLLATKT TARKELILLH DERTVAPGST RQWLSNRPWI QTHYHVELPG
1260 1270 1280 1290 1300
VVTPARPIPP VHDAAAIAAF KHLREQVETR IKKYRGLRPF TRPRRPPHMN
1310 1320 1330 1340 1350
DFARIARRLC GQQIGLVLGG GGARGISHIG MLQALEEFGI PIDAIGGCSI
1360 1370 1380 1390 1400
GSFVGGLYAR ETDLLETAGR TKQFSGRMGS MWRILSDVTY PFVSYTTGHE
1410 1420 1430 1440 1450
FNRGIYKAFY NTHIEDFWIP FFANSTNITH SRMEVHRTGY AWRYVRASMT
1460 1470 1480 1490 1500
LAGLLPPLSD NGNLLVDGGY MDNTPIQPLR ENGIRDIIVV DVGSVDDTSP
1510 1520 1530 1540 1550
RDYGDSVSGW WIFFNRFNPF YERRVLSMTE ISSRLTYVSS VKTLEGVKAT
1560 1570 1580 1590 1600
PGCHYIAMPV QQFDTLGGFK RFSEVMEIGL KAGRETLKKW KEEGKLPTGL
1610 1620
VDEAKGSKAV QRGNRLRRMS I
Length:1,621
Mass (Da):178,011
Last modified:June 28, 2011 - v1
Checksum:i0F00F6E602754505
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEY01000019 Genomic DNA. Translation: EAL21520.1.
RefSeqiXP_776167.1. XM_771074.1.

Genome annotation databases

EnsemblFungiiEAL21520; EAL21520; CNBD2140.
GeneIDi4935603.
KEGGicnb:CNBD2140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEY01000019 Genomic DNA. Translation: EAL21520.1.
RefSeqiXP_776167.1. XM_771074.1.

3D structure databases

ProteinModelPortaliP0CP37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi283643.XP_776167.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAL21520; EAL21520; CNBD2140.
GeneIDi4935603.
KEGGicnb:CNBD2140.

Organism-specific databases

EuPathDBiFungiDB:CNBD2140.

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
HOGENOMiHOG000048680.
KOiK14676.
OrthoDBiEOG092C0BAH.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 5 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTE1_CRYNB
AccessioniPrimary (citable) accession number: P0CP37
Secondary accession number(s): Q55U92, Q5KI53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.