Histone acetyltransferase ESA1 - Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Histone acetyltransferase ESA1
EC=2.3.1.48

Gene names

Name:	ESA1
Ordered Locus Names:	CNBB2530

Organism

Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

283643 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex ›

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. Acetylation of histone H4 is essential for DNA double-strand break repair through homologous recombination. Involved in cell cycle progression. Recruitment to promoters depends on H3K4me By similarity.
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subunit structure	Component of the NuA4 histone acetyltransferase complex By similarity.
Subcellular location	Nucleus By similarity.
Domain	The ESA1-RPD3 motif is common to ESA1 and RPD3 and is required for ESA1 histone acetyl-transferase (HAT) activity and RPD3 histone deacetylase (HDAC) activity.
Post-translational modification	Autoacetylation at Lys-381 is required for proper function By similarity.
Sequence similarities	Belongs to the MYST (SAS/MOZ) family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Chromatin regulator Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histone acetylation Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 564

564

Histone acetyltransferase ESA1

PRO_0000410158

Regions

Region

431 – 437

7

Acetyl-CoA binding By similarity

Motif

364 – 385

22

ESA1-RPD3 motif By similarity

Compositional bias

65 – 85

21

Pro-rich

Compositional bias

121 – 199

79

Lys-rich

Sites

Active site

381

1

By similarity

Active site

423

1

Nucleophile By similarity

Binding site

426

1

Acetyl-CoA By similarity

Binding site

461

1

Acetyl-CoA By similarity

Amino acid modifications

Modified residue

381

1

N6-acetyllysine; by autocatalysis By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P0CP03 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 76BE79DB995BF692
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FASTA

564

63,050

        10         20         30         40         50         60 
MSPSAPSTPH GRGSGSEPGT PAPSVAAGGS YTIDDVVPGV KIYVIKPLSN GQAEQRRAEI 

        70         80         90        100        110        120 
LSTRPKPKPS AFAPPPPPNA PSPDPRDDTE YYVHYVEFNK RLDEWVGGSR LVLSKEMEWP 

       130        140        150        160        170        180 
KSKDEPKKKD RPAKAQPSKA PSRATGSPIP SDSLLKKAAN KAAMAAGKAT PGKAMPSSKL 

       190        200        210        220        230        240 
GKASKIGKAG KFPQKRKAKT EADTEAEEES NEDNDALGEE EDMDEDGDVT LITSDGAIDP 

       250        260        270        280        290        300 
SREVVAAPSN PRAAPQVFSK KQEIEKLRTS GSMTQSHSEI SRVKNLNKLQ IGKHEVETWY 

       310        320        330        340        350        360 
FSPYPIEYAH LPVLYICEFC LLYYPSATQL RRHRAKCTLL HPPGNEIYRH EGISFFEIDG 

       370        380        390        400        410        420 
RKQRTWCRNL CLISKCFLDH KTLYYDVDPF LYYCMTVKDD YGCHLIGYFS KEKESAEGYN 

       430        440        450        460        470        480 
VACILTLPQH QRKGYGRLLI EFSYELSKVE GKLGSPEKPL SDLGLLGYRA YWQEKIVELL 

       490        500        510        520        530        540 
LDSDYEISLD EIAQKTSITH GDIMHTCQAL QMIKYYKNSH IIHLTDAVIE QHKKTKAKPR 

       550        560 
RAINPAYLKW KPPVFSRAQL AFGF

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References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.

Hyman R.W.
Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B-3501A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAEY01000009 Genomic DNA. Translation: EAL22621.1.
RefSeq	XP_777268.1. XM_772175.1.
3D structure databases
ProteinModelPortal	P0CP03.
SMR	P0CP03. Positions 281-553.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4934345.
KEGG	cnb:CNBB2530.
Phylogenomic databases
HOGENOM	HOG000182457.
KO	K11304.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. IPR002717. MOZ_SAS. IPR025995. Tudor-knot. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF01853. MOZ_SAS. 1 hit. PF11717. Tudor-knot. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit. SSF54160. Chromodomain-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ESA1_CRYNB

Accession

Primary (citable) accession number: P0CP03
Secondary accession number(s): Q55XW1, Q5KM33

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	March 6, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families