P0CN26 (DPOE_CRYNJ) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 11.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: DNA polymerase epsilon catalytic subunit A EC=2.7.7.7 Alternative name(s): DNA polymerase II subunit A | ||||
| Gene names |
| ||||
| Organism | Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome] | ||||
| Taxonomic identifier | 214684 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex ›  |
Protein attributes
| Sequence length | 2250 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | DNA polymerase II participates in chromosomal DNA replication By similarity. |
| Catalytic activity | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). |
| Cofactor | Binds 1 4Fe-4S cluster By similarity. |
| Subunit structure | Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4 By similarity. |
| Subcellular location | Nucleus By similarity. |
| Domain | The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C By similarity. The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes By similarity. |
| Sequence similarities | Belongs to the DNA polymerase type-B family. Contains 1 CysA-type zinc finger. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication |
| Cellular component | Nucleus |
| Domain | Zinc-finger |
| Ligand | 4Fe-4S DNA-binding Iron Iron-sulfur Metal-binding Zinc |
| Molecular function | DNA-directed DNA polymerase Nucleotidyltransferase Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | DNA replication Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW DNA bindingInferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activityInferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 2250 | 2250 | DNA polymerase epsilon catalytic subunit A | PRO_0000046460 | |||||
Regions | |||||||||
| Zinc finger | 2118 – 2159 | 42 | CysA-type | ||||||
| Motif | 2190 – 2207 | 18 | CysB motif | ||||||
| Compositional bias | 5 – 43 | 39 | Gly-rich | ||||||
Sites | |||||||||
| Metal binding | 2118 | 1 | Zinc By similarity | ||||||
| Metal binding | 2121 | 1 | Zinc By similarity | ||||||
| Metal binding | 2156 | 1 | Zinc By similarity | ||||||
| Metal binding | 2159 | 1 | Zinc By similarity | ||||||
| Metal binding | 2190 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 2193 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 2205 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 2207 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Sequences
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References
| [1] | "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.  Hyman R.W.Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JEC21 / ATCC MYA-565. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE017351 Genomic DNA. Translation: AAW46304.1. |
| RefSeq | XP_567821.1. XM_567821.1. |
| UniGene | Fne.4301. |
3D structure databases | |
| ProteinModelPortal | P0CN26. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3254656. |
| KEGG | cne:CNK01430. |
Phylogenomic databases | |
| KO | K02324. |
Family and domain databases | |
| InterPro | IPR006172. DNA-dir_DNA_pol_B. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006134. DNA-dir_DNA_pol_B_multi_dom. IPR013697. DNA_pol_e_suA_C. IPR012337. RNaseH-like_dom. [Graphical view] |
| Pfam | PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo1. 1 hit. PF08490. DUF1744. 1 hit. [Graphical view] |
| SMART | SM00486. POLBc. 1 hit. [Graphical view] |
| SUPFAM | SSF53098. RNaseH_fold. 1 hit. |
| PROSITE | PS00116. DNA_POLYMERASE_B. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DPOE_CRYNJ | ||||||||
| Accession | Primary (citable) accession number: P0CN26 Secondary accession number(s): Q55JX3, Q5K9M7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |