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P0CN26 (DPOE_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase epsilon catalytic subunit A

EC=2.7.7.7
Alternative name(s):
DNA polymerase II subunit A
Gene names
Name:POL2
Ordered Locus Names:CNK01430
OrganismCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome]
Taxonomic identifier214684 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length2250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase II participates in chromosomal DNA replication By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactor

Binds 1 4Fe-4S cluster By similarity.

Subunit structure

Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4 By similarity.

Subcellular location

Nucleus By similarity.

Domain

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C By similarity.

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 1 CysA-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 22502250DNA polymerase epsilon catalytic subunit A
PRO_0000046460

Regions

Zinc finger2118 – 215942CysA-type
Motif2190 – 220718CysB motif
Compositional bias5 – 4339Gly-rich

Sites

Metal binding21181Zinc By similarity
Metal binding21211Zinc By similarity
Metal binding21561Zinc By similarity
Metal binding21591Zinc By similarity
Metal binding21901Iron-sulfur (4Fe-4S) By similarity
Metal binding21931Iron-sulfur (4Fe-4S) By similarity
Metal binding22051Iron-sulfur (4Fe-4S) By similarity
Metal binding22071Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P0CN26 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 30EED4407247B80B

FASTA2,250255,999
        10         20         30         40         50         60 
MPSRGSFRGR GRGSGSGFGS NTRFTGRRRG RGGGGGGGGV AYGIDRRPAT STNQANREDG 

        70         80         90        100        110        120 
TAATEKFEEV KVYDEIDEKI GFWRFESVRA EGEEKVGWLV NMHQTLVPSD VHPGGLAAVD 

       130        140        150        160        170        180 
YYFIQDDGGS FKVSIPYEPY FYLTCRGGTE SIVEEWLLKR YEGIIIRIER EKKWDLSLPN 

       190        200        210        220        230        240 
HLLSAPPVFL KLFFHNTADL QTIRRDLLPL ARSNSEKFTA VDAYADVVSA EAAANGHGDD 

       250        260        270        280        290        300 
ENRAWGAEDD TKKKKDKEPS ECIIEVREHD LAYHLRVAID LNIRVGLWYT VTSRTGVITL 

       310        320        330        340        350        360 
ERIPDRVKRA DPVVMAYDIE TTKQPLKFPD QQTDQIMMIS YMIDGMGYLI TNREIVGEDI 

       370        380        390        400        410        420 
DDFEYTPKDE YPGEFTVFNE PDEPAVIRRW FEHIRDSKPT VIATYNGDSF DFPFVDARAK 

       430        440        450        460        470        480 
IHGISMYEEI GFKPDIEEEY KSRSTMHMDC FRWVKRDSYL PQGSQGLKAV TTAKLGYNPI 

       490        500        510        520        530        540 
ELDPELMTPY AIEQPQILAQ YSVSDAVATY YLYMKYVHPF IFSLCNIIPL SPDEVLRKGT 

       550        560        570        580        590        600 
GTLCETLLMV EAYDAHIIMP NRHEDPHGVT YEGHLLASET YVGGHVEALE AGVFRSDIPT 

       610        620        630        640        650        660 
HFKIVPSAIQ ELIDDLDAAL RFSLIEEGQV KLEDVENYDE VKQQIQTALE TMRDEPNRFD 

       670        680        690        700        710        720 
NPLIYHLDVA AMYPNIMLSN RLQPDSVKEE ADCAVCDYNR PDKKCDRRME WAWRGEYFPA 

       730        740        750        760        770        780 
KRDEVNMVRY ALEQETFPPK RPYDPKRRFV DLTPTEQSAL LHKRLGDYSR KVYKKTHDTK 

       790        800        810        820        830        840 
IVTKTTIICQ RENSFYIDTV RAFRDRRYEY KGLHKTWKKN LDKAFEEGGA VATVDEAKKM 

       850        860        870        880        890        900 
IVLYDSLQLA HKCILNSFYG YVMRKGARWY SMEMAGITCL TGATIIQMAR QLVEQIGRPL 

       910        920        930        940        950        960 
ELDTDGIWCM LPGVFPEDFN FKLKNGKKFG ISYPCTMLNH LVHAQFTNDQ YHELVNNDSG 

       970        980        990       1000       1010       1020 
TYNVKKENSI FFELDGPYKA MILPSSKEED KLLKKRYAVF NPDGSLAELK GFEVKRRGEL 

      1030       1040       1050       1060       1070       1080 
QMIKIFQSQI FDKFLLGKTT EECYAAVATV ADQWLDILQS KASSLHDDEL VDLIAENRSM 

      1090       1100       1110       1120       1130       1140 
SKTLAEYAGQ KSTSISTARR LAEFLGEQMV KDKGLSCRFI ISAKPNGAPV TERAVPVAIF 

      1150       1160       1170       1180       1190       1200 
TAEEPVKRHY LRKWLKDNSL TDFDLRTILD WDYYTERLGS VIQKLITIPA ALQKVPNPVP 

      1210       1220       1230       1240       1250       1260 
RIRHPDWLYK RIATKEDKFQ QHKITDMFTK LKDMEDLGDG QKKVGPKLAV VRRRNRKEQE 

      1270       1280       1290       1300       1310       1320 
KESEVEEVPP EPEYDYAGYI RIMKKKWRKQ RQEKARARKS GLRQDGTISS MLRTQTSSMN 

      1330       1340       1350       1360       1370       1380 
SKQWDVIQIA STNRPGEFKL WLAIDGTFQS VRLKVPREFY LNFKEDPEPQ MLATDRYEAV 

      1390       1400       1410       1420       1430       1440 
EVVRTLPRGQ PARHLYKLSV DEVLFMEGES HFSTLINNPN VDGAFELQVP LVVRALLSLG 

      1450       1460       1470       1480       1490       1500 
TSCALRSNIL GGLNRGLDKG FDLVDLERSG NLSRRKYLNE GRGIRYHFLF HAVADQRHII 

      1510       1520       1530       1540       1550       1560 
GLFSPDSTNA SIYLVDRAKN RQQLPNPLKF YTDRVERAER GVFSYPDMLD FTTSYHSSES 

      1570       1580       1590       1600       1610       1620 
SAFKALGKDL QAINHGLNVI ALCSPFEHSY YQAKAPVYSN FPFITYRLGK EEDPGLMWLL 

      1630       1640       1650       1660       1670       1680 
QTSRRMIGLY LRLSSWLKEQ IQIASHFDVP IGNLGADIAV FLADIEFARR LKQQDMLIWW 

      1690       1700       1710       1720       1730       1740 
SSTPRPDLGG SEEDANSSED LVSPHISNRG CYSSTVLEME VSDLAINAVL QSALVNEMEG 

      1750       1760       1770       1780       1790       1800 
SGTGSLAFDS ASHNLDEYAK GAVNTSVMLG DAVLSTQTFG VLKSMLRAWY ADKARAHVKG 

      1810       1820       1830       1840       1850       1860 
DSLSPAEIVV DQFWRWISSS TSSMFEPALH RFLHGLMRKT FLQLLAEFKR LGTQVVYADF 

      1870       1880       1890       1900       1910       1920 
GRVFLLTSKP DAGSAFAFAK YLVAAANSQE LFRHLIIDVA QFWNYLAWMD VANFGGVKVS 

      1930       1940       1950       1960       1970       1980 
PETAASREPP AKRFEISMDW NIQSFLPGTL QPVFERNVAS FIFSLYSAKR SSSDGREPLR 

      1990       2000       2010       2020       2030       2040 
VIHSLNIDQP GTEATSTMNP TKEKEKKAAS KSISQTLTRR LLSDIAAVKR QQAAVHLEPE 

      2050       2060       2070       2080       2090       2100 
EAYTLAFPDL PGARSKRINP TLELIKAITE VYSLASEHSI EVQILKRNLL DLIGVKEFSS 

      2110       2120       2130       2140       2150       2160 
DAAFNPPCES IEVPMVICKK CNAIRDVDLC RDPDRLPSVN PDSGEMLEPA RKNWVCHKCD 

      2170       2180       2190       2200       2210       2220 
SEYDRFQIEQ PLIEMVTKTI TNYQIQDVIC LKCSQTKSDN LAATCKCGGG FRTTSNRNEL 

      2230       2240       2250 
KTKLKMIKSV CDYHKLPFAG SYVEETLSRW 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017351 Genomic DNA. Translation: AAW46304.1.
RefSeqXP_567821.1. XM_567821.1.
UniGeneFne.4301.

3D structure databases

ProteinModelPortalP0CN26.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAW46304; AAW46304; CNK01430.
GeneID3254656.
KEGGcne:CNK01430.

Phylogenomic databases

KOK02324.
OrthoDBEOG790G80.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view]
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 2 hits.
ProtoNetSearch...

Entry information

Entry nameDPOE_CRYNJ
AccessionPrimary (citable) accession number: P0CN26
Secondary accession number(s): Q55JX3, Q5K9M7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families