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P0CN26

- DPOE_CRYNJ

UniProt

P0CN26 - DPOE_CRYNJ

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Protein

DNA polymerase epsilon catalytic subunit A

Gene

POL2

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA polymerase II participates in chromosomal DNA replication.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Binds 1 4Fe-4S cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi2118 – 21181ZincBy similarity
Metal bindingi2121 – 21211ZincBy similarity
Metal bindingi2156 – 21561ZincBy similarity
Metal bindingi2159 – 21591ZincBy similarity
Metal bindingi2190 – 21901Iron-sulfur (4Fe-4S)By similarity
Metal bindingi2193 – 21931Iron-sulfur (4Fe-4S)By similarity
Metal bindingi2205 – 22051Iron-sulfur (4Fe-4S)By similarity
Metal bindingi2207 – 22071Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2118 – 215942CysA-typeAdd
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit A
Gene namesi
Name:POL2
Ordered Locus Names:CNK01430
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000002149: Chromosome 11

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. epsilon DNA polymerase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22502250DNA polymerase epsilon catalytic subunit APRO_0000046460Add
BLAST

Interactioni

Subunit structurei

Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0CN26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2190 – 220718CysB motifAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 4339Gly-richAdd
BLAST

Domaini

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C.By similarity
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 1 CysA-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2118 – 215942CysA-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiP0CN26.
KOiK02324.
OrthoDBiEOG790G80.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR029703. POL2.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10670:SF0. PTHR10670:SF0. 1 hit.
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view]
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.

Sequencei

Sequence statusi: Complete.

P0CN26-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSRGSFRGR GRGSGSGFGS NTRFTGRRRG RGGGGGGGGV AYGIDRRPAT
60 70 80 90 100
STNQANREDG TAATEKFEEV KVYDEIDEKI GFWRFESVRA EGEEKVGWLV
110 120 130 140 150
NMHQTLVPSD VHPGGLAAVD YYFIQDDGGS FKVSIPYEPY FYLTCRGGTE
160 170 180 190 200
SIVEEWLLKR YEGIIIRIER EKKWDLSLPN HLLSAPPVFL KLFFHNTADL
210 220 230 240 250
QTIRRDLLPL ARSNSEKFTA VDAYADVVSA EAAANGHGDD ENRAWGAEDD
260 270 280 290 300
TKKKKDKEPS ECIIEVREHD LAYHLRVAID LNIRVGLWYT VTSRTGVITL
310 320 330 340 350
ERIPDRVKRA DPVVMAYDIE TTKQPLKFPD QQTDQIMMIS YMIDGMGYLI
360 370 380 390 400
TNREIVGEDI DDFEYTPKDE YPGEFTVFNE PDEPAVIRRW FEHIRDSKPT
410 420 430 440 450
VIATYNGDSF DFPFVDARAK IHGISMYEEI GFKPDIEEEY KSRSTMHMDC
460 470 480 490 500
FRWVKRDSYL PQGSQGLKAV TTAKLGYNPI ELDPELMTPY AIEQPQILAQ
510 520 530 540 550
YSVSDAVATY YLYMKYVHPF IFSLCNIIPL SPDEVLRKGT GTLCETLLMV
560 570 580 590 600
EAYDAHIIMP NRHEDPHGVT YEGHLLASET YVGGHVEALE AGVFRSDIPT
610 620 630 640 650
HFKIVPSAIQ ELIDDLDAAL RFSLIEEGQV KLEDVENYDE VKQQIQTALE
660 670 680 690 700
TMRDEPNRFD NPLIYHLDVA AMYPNIMLSN RLQPDSVKEE ADCAVCDYNR
710 720 730 740 750
PDKKCDRRME WAWRGEYFPA KRDEVNMVRY ALEQETFPPK RPYDPKRRFV
760 770 780 790 800
DLTPTEQSAL LHKRLGDYSR KVYKKTHDTK IVTKTTIICQ RENSFYIDTV
810 820 830 840 850
RAFRDRRYEY KGLHKTWKKN LDKAFEEGGA VATVDEAKKM IVLYDSLQLA
860 870 880 890 900
HKCILNSFYG YVMRKGARWY SMEMAGITCL TGATIIQMAR QLVEQIGRPL
910 920 930 940 950
ELDTDGIWCM LPGVFPEDFN FKLKNGKKFG ISYPCTMLNH LVHAQFTNDQ
960 970 980 990 1000
YHELVNNDSG TYNVKKENSI FFELDGPYKA MILPSSKEED KLLKKRYAVF
1010 1020 1030 1040 1050
NPDGSLAELK GFEVKRRGEL QMIKIFQSQI FDKFLLGKTT EECYAAVATV
1060 1070 1080 1090 1100
ADQWLDILQS KASSLHDDEL VDLIAENRSM SKTLAEYAGQ KSTSISTARR
1110 1120 1130 1140 1150
LAEFLGEQMV KDKGLSCRFI ISAKPNGAPV TERAVPVAIF TAEEPVKRHY
1160 1170 1180 1190 1200
LRKWLKDNSL TDFDLRTILD WDYYTERLGS VIQKLITIPA ALQKVPNPVP
1210 1220 1230 1240 1250
RIRHPDWLYK RIATKEDKFQ QHKITDMFTK LKDMEDLGDG QKKVGPKLAV
1260 1270 1280 1290 1300
VRRRNRKEQE KESEVEEVPP EPEYDYAGYI RIMKKKWRKQ RQEKARARKS
1310 1320 1330 1340 1350
GLRQDGTISS MLRTQTSSMN SKQWDVIQIA STNRPGEFKL WLAIDGTFQS
1360 1370 1380 1390 1400
VRLKVPREFY LNFKEDPEPQ MLATDRYEAV EVVRTLPRGQ PARHLYKLSV
1410 1420 1430 1440 1450
DEVLFMEGES HFSTLINNPN VDGAFELQVP LVVRALLSLG TSCALRSNIL
1460 1470 1480 1490 1500
GGLNRGLDKG FDLVDLERSG NLSRRKYLNE GRGIRYHFLF HAVADQRHII
1510 1520 1530 1540 1550
GLFSPDSTNA SIYLVDRAKN RQQLPNPLKF YTDRVERAER GVFSYPDMLD
1560 1570 1580 1590 1600
FTTSYHSSES SAFKALGKDL QAINHGLNVI ALCSPFEHSY YQAKAPVYSN
1610 1620 1630 1640 1650
FPFITYRLGK EEDPGLMWLL QTSRRMIGLY LRLSSWLKEQ IQIASHFDVP
1660 1670 1680 1690 1700
IGNLGADIAV FLADIEFARR LKQQDMLIWW SSTPRPDLGG SEEDANSSED
1710 1720 1730 1740 1750
LVSPHISNRG CYSSTVLEME VSDLAINAVL QSALVNEMEG SGTGSLAFDS
1760 1770 1780 1790 1800
ASHNLDEYAK GAVNTSVMLG DAVLSTQTFG VLKSMLRAWY ADKARAHVKG
1810 1820 1830 1840 1850
DSLSPAEIVV DQFWRWISSS TSSMFEPALH RFLHGLMRKT FLQLLAEFKR
1860 1870 1880 1890 1900
LGTQVVYADF GRVFLLTSKP DAGSAFAFAK YLVAAANSQE LFRHLIIDVA
1910 1920 1930 1940 1950
QFWNYLAWMD VANFGGVKVS PETAASREPP AKRFEISMDW NIQSFLPGTL
1960 1970 1980 1990 2000
QPVFERNVAS FIFSLYSAKR SSSDGREPLR VIHSLNIDQP GTEATSTMNP
2010 2020 2030 2040 2050
TKEKEKKAAS KSISQTLTRR LLSDIAAVKR QQAAVHLEPE EAYTLAFPDL
2060 2070 2080 2090 2100
PGARSKRINP TLELIKAITE VYSLASEHSI EVQILKRNLL DLIGVKEFSS
2110 2120 2130 2140 2150
DAAFNPPCES IEVPMVICKK CNAIRDVDLC RDPDRLPSVN PDSGEMLEPA
2160 2170 2180 2190 2200
RKNWVCHKCD SEYDRFQIEQ PLIEMVTKTI TNYQIQDVIC LKCSQTKSDN
2210 2220 2230 2240 2250
LAATCKCGGG FRTTSNRNEL KTKLKMIKSV CDYHKLPFAG SYVEETLSRW
Length:2,250
Mass (Da):255,999
Last modified:June 28, 2011 - v1
Checksum:i30EED4407247B80B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017351 Genomic DNA. Translation: AAW46304.1.
RefSeqiXP_567821.1. XM_567821.1.
UniGeneiFne.4301.

Genome annotation databases

EnsemblFungiiAAW46304; AAW46304; CNK01430.
GeneIDi3254656.
KEGGicne:CNK01430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017351 Genomic DNA. Translation: AAW46304.1 .
RefSeqi XP_567821.1. XM_567821.1.
UniGenei Fne.4301.

3D structure databases

ProteinModelPortali P0CN26.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii AAW46304 ; AAW46304 ; CNK01430 .
GeneIDi 3254656.
KEGGi cne:CNK01430.

Phylogenomic databases

InParanoidi P0CN26.
KOi K02324.
OrthoDBi EOG790G80.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR029703. POL2.
IPR012337. RNaseH-like_dom.
[Graphical view ]
PANTHERi PTHR10670:SF0. PTHR10670:SF0. 1 hit.
Pfami PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view ]
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
    Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
    , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
    Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JEC21 / ATCC MYA-565.

Entry informationi

Entry nameiDPOE_CRYNJ
AccessioniPrimary (citable) accession number: P0CN26
Secondary accession number(s): Q55JX3, Q5K9M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3