Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CN26

- DPOE_CRYNJ

UniProt

P0CN26 - DPOE_CRYNJ

Protein

DNA polymerase epsilon catalytic subunit A

Gene

POL2

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 18 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA polymerase II participates in chromosomal DNA replication.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    Cofactori

    Binds 1 4Fe-4S cluster.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi2118 – 21181ZincBy similarity
    Metal bindingi2121 – 21211ZincBy similarity
    Metal bindingi2156 – 21561ZincBy similarity
    Metal bindingi2159 – 21591ZincBy similarity
    Metal bindingi2190 – 21901Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi2193 – 21931Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi2205 – 22051Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi2207 – 22071Iron-sulfur (4Fe-4S)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri2118 – 215942CysA-typeAdd
    BLAST

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    4. nucleotide binding Source: InterPro
    5. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
    Alternative name(s):
    DNA polymerase II subunit A
    Gene namesi
    Name:POL2
    Ordered Locus Names:CNK01430
    OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
    Taxonomic identifieri214684 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000002149: Chromosome 11

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22502250DNA polymerase epsilon catalytic subunit APRO_0000046460Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0CN26.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi2190 – 220718CysB motifAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi5 – 4339Gly-richAdd
    BLAST

    Domaini

    The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C.By similarity
    The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated
    Contains 1 CysA-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri2118 – 215942CysA-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    KOiK02324.
    OrthoDBiEOG790G80.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR013697. DNA_pol_e_suA_C.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 1 hit.
    PF08490. DUF1744. 1 hit.
    [Graphical view]
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P0CN26-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSRGSFRGR GRGSGSGFGS NTRFTGRRRG RGGGGGGGGV AYGIDRRPAT     50
    STNQANREDG TAATEKFEEV KVYDEIDEKI GFWRFESVRA EGEEKVGWLV 100
    NMHQTLVPSD VHPGGLAAVD YYFIQDDGGS FKVSIPYEPY FYLTCRGGTE 150
    SIVEEWLLKR YEGIIIRIER EKKWDLSLPN HLLSAPPVFL KLFFHNTADL 200
    QTIRRDLLPL ARSNSEKFTA VDAYADVVSA EAAANGHGDD ENRAWGAEDD 250
    TKKKKDKEPS ECIIEVREHD LAYHLRVAID LNIRVGLWYT VTSRTGVITL 300
    ERIPDRVKRA DPVVMAYDIE TTKQPLKFPD QQTDQIMMIS YMIDGMGYLI 350
    TNREIVGEDI DDFEYTPKDE YPGEFTVFNE PDEPAVIRRW FEHIRDSKPT 400
    VIATYNGDSF DFPFVDARAK IHGISMYEEI GFKPDIEEEY KSRSTMHMDC 450
    FRWVKRDSYL PQGSQGLKAV TTAKLGYNPI ELDPELMTPY AIEQPQILAQ 500
    YSVSDAVATY YLYMKYVHPF IFSLCNIIPL SPDEVLRKGT GTLCETLLMV 550
    EAYDAHIIMP NRHEDPHGVT YEGHLLASET YVGGHVEALE AGVFRSDIPT 600
    HFKIVPSAIQ ELIDDLDAAL RFSLIEEGQV KLEDVENYDE VKQQIQTALE 650
    TMRDEPNRFD NPLIYHLDVA AMYPNIMLSN RLQPDSVKEE ADCAVCDYNR 700
    PDKKCDRRME WAWRGEYFPA KRDEVNMVRY ALEQETFPPK RPYDPKRRFV 750
    DLTPTEQSAL LHKRLGDYSR KVYKKTHDTK IVTKTTIICQ RENSFYIDTV 800
    RAFRDRRYEY KGLHKTWKKN LDKAFEEGGA VATVDEAKKM IVLYDSLQLA 850
    HKCILNSFYG YVMRKGARWY SMEMAGITCL TGATIIQMAR QLVEQIGRPL 900
    ELDTDGIWCM LPGVFPEDFN FKLKNGKKFG ISYPCTMLNH LVHAQFTNDQ 950
    YHELVNNDSG TYNVKKENSI FFELDGPYKA MILPSSKEED KLLKKRYAVF 1000
    NPDGSLAELK GFEVKRRGEL QMIKIFQSQI FDKFLLGKTT EECYAAVATV 1050
    ADQWLDILQS KASSLHDDEL VDLIAENRSM SKTLAEYAGQ KSTSISTARR 1100
    LAEFLGEQMV KDKGLSCRFI ISAKPNGAPV TERAVPVAIF TAEEPVKRHY 1150
    LRKWLKDNSL TDFDLRTILD WDYYTERLGS VIQKLITIPA ALQKVPNPVP 1200
    RIRHPDWLYK RIATKEDKFQ QHKITDMFTK LKDMEDLGDG QKKVGPKLAV 1250
    VRRRNRKEQE KESEVEEVPP EPEYDYAGYI RIMKKKWRKQ RQEKARARKS 1300
    GLRQDGTISS MLRTQTSSMN SKQWDVIQIA STNRPGEFKL WLAIDGTFQS 1350
    VRLKVPREFY LNFKEDPEPQ MLATDRYEAV EVVRTLPRGQ PARHLYKLSV 1400
    DEVLFMEGES HFSTLINNPN VDGAFELQVP LVVRALLSLG TSCALRSNIL 1450
    GGLNRGLDKG FDLVDLERSG NLSRRKYLNE GRGIRYHFLF HAVADQRHII 1500
    GLFSPDSTNA SIYLVDRAKN RQQLPNPLKF YTDRVERAER GVFSYPDMLD 1550
    FTTSYHSSES SAFKALGKDL QAINHGLNVI ALCSPFEHSY YQAKAPVYSN 1600
    FPFITYRLGK EEDPGLMWLL QTSRRMIGLY LRLSSWLKEQ IQIASHFDVP 1650
    IGNLGADIAV FLADIEFARR LKQQDMLIWW SSTPRPDLGG SEEDANSSED 1700
    LVSPHISNRG CYSSTVLEME VSDLAINAVL QSALVNEMEG SGTGSLAFDS 1750
    ASHNLDEYAK GAVNTSVMLG DAVLSTQTFG VLKSMLRAWY ADKARAHVKG 1800
    DSLSPAEIVV DQFWRWISSS TSSMFEPALH RFLHGLMRKT FLQLLAEFKR 1850
    LGTQVVYADF GRVFLLTSKP DAGSAFAFAK YLVAAANSQE LFRHLIIDVA 1900
    QFWNYLAWMD VANFGGVKVS PETAASREPP AKRFEISMDW NIQSFLPGTL 1950
    QPVFERNVAS FIFSLYSAKR SSSDGREPLR VIHSLNIDQP GTEATSTMNP 2000
    TKEKEKKAAS KSISQTLTRR LLSDIAAVKR QQAAVHLEPE EAYTLAFPDL 2050
    PGARSKRINP TLELIKAITE VYSLASEHSI EVQILKRNLL DLIGVKEFSS 2100
    DAAFNPPCES IEVPMVICKK CNAIRDVDLC RDPDRLPSVN PDSGEMLEPA 2150
    RKNWVCHKCD SEYDRFQIEQ PLIEMVTKTI TNYQIQDVIC LKCSQTKSDN 2200
    LAATCKCGGG FRTTSNRNEL KTKLKMIKSV CDYHKLPFAG SYVEETLSRW 2250
    Length:2,250
    Mass (Da):255,999
    Last modified:June 28, 2011 - v1
    Checksum:i30EED4407247B80B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017351 Genomic DNA. Translation: AAW46304.1.
    RefSeqiXP_567821.1. XM_567821.1.
    UniGeneiFne.4301.

    Genome annotation databases

    EnsemblFungiiAAW46304; AAW46304; CNK01430.
    GeneIDi3254656.
    KEGGicne:CNK01430.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017351 Genomic DNA. Translation: AAW46304.1 .
    RefSeqi XP_567821.1. XM_567821.1.
    UniGenei Fne.4301.

    3D structure databases

    ProteinModelPortali P0CN26.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii AAW46304 ; AAW46304 ; CNK01430 .
    GeneIDi 3254656.
    KEGGi cne:CNK01430.

    Phylogenomic databases

    KOi K02324.
    OrthoDBi EOG790G80.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR013697. DNA_pol_e_suA_C.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 1 hit.
    PF08490. DUF1744. 1 hit.
    [Graphical view ]
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
      Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
      , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
      Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JEC21 / ATCC MYA-565.

    Entry informationi

    Entry nameiDPOE_CRYNJ
    AccessioniPrimary (citable) accession number: P0CN26
    Secondary accession number(s): Q55JX3, Q5K9M7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3