SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0CN26

- DPOE_CRYNJ

UniProt

P0CN26 - DPOE_CRYNJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA polymerase epsilon catalytic subunit A

Gene
POL2, CNK01430
Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA polymerase II participates in chromosomal DNA replication By similarity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Binds 1 4Fe-4S cluster By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi2118 – 21181Zinc By similarity
Metal bindingi2121 – 21211Zinc By similarity
Metal bindingi2156 – 21561Zinc By similarity
Metal bindingi2159 – 21591Zinc By similarity
Metal bindingi2190 – 21901Iron-sulfur (4Fe-4S) By similarity
Metal bindingi2193 – 21931Iron-sulfur (4Fe-4S) By similarity
Metal bindingi2205 – 22051Iron-sulfur (4Fe-4S) By similarity
Metal bindingi2207 – 22071Iron-sulfur (4Fe-4S) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2118 – 215942CysA-typeAdd
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro
  5. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit A
Gene namesi
Name:POL2
Ordered Locus Names:CNK01430
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000002149: Chromosome 11

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22502250DNA polymerase epsilon catalytic subunit APRO_0000046460Add
BLAST

Interactioni

Subunit structurei

Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0CN26.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2190 – 220718CysB motifAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 4339Gly-richAdd
BLAST

Domaini

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C By similarity.
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes By similarity.

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

KOiK02324.
OrthoDBiEOG790G80.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view]
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.

Sequencei

Sequence statusi: Complete.

P0CN26-1 [UniParc]FASTAAdd to Basket

« Hide

MPSRGSFRGR GRGSGSGFGS NTRFTGRRRG RGGGGGGGGV AYGIDRRPAT     50
STNQANREDG TAATEKFEEV KVYDEIDEKI GFWRFESVRA EGEEKVGWLV 100
NMHQTLVPSD VHPGGLAAVD YYFIQDDGGS FKVSIPYEPY FYLTCRGGTE 150
SIVEEWLLKR YEGIIIRIER EKKWDLSLPN HLLSAPPVFL KLFFHNTADL 200
QTIRRDLLPL ARSNSEKFTA VDAYADVVSA EAAANGHGDD ENRAWGAEDD 250
TKKKKDKEPS ECIIEVREHD LAYHLRVAID LNIRVGLWYT VTSRTGVITL 300
ERIPDRVKRA DPVVMAYDIE TTKQPLKFPD QQTDQIMMIS YMIDGMGYLI 350
TNREIVGEDI DDFEYTPKDE YPGEFTVFNE PDEPAVIRRW FEHIRDSKPT 400
VIATYNGDSF DFPFVDARAK IHGISMYEEI GFKPDIEEEY KSRSTMHMDC 450
FRWVKRDSYL PQGSQGLKAV TTAKLGYNPI ELDPELMTPY AIEQPQILAQ 500
YSVSDAVATY YLYMKYVHPF IFSLCNIIPL SPDEVLRKGT GTLCETLLMV 550
EAYDAHIIMP NRHEDPHGVT YEGHLLASET YVGGHVEALE AGVFRSDIPT 600
HFKIVPSAIQ ELIDDLDAAL RFSLIEEGQV KLEDVENYDE VKQQIQTALE 650
TMRDEPNRFD NPLIYHLDVA AMYPNIMLSN RLQPDSVKEE ADCAVCDYNR 700
PDKKCDRRME WAWRGEYFPA KRDEVNMVRY ALEQETFPPK RPYDPKRRFV 750
DLTPTEQSAL LHKRLGDYSR KVYKKTHDTK IVTKTTIICQ RENSFYIDTV 800
RAFRDRRYEY KGLHKTWKKN LDKAFEEGGA VATVDEAKKM IVLYDSLQLA 850
HKCILNSFYG YVMRKGARWY SMEMAGITCL TGATIIQMAR QLVEQIGRPL 900
ELDTDGIWCM LPGVFPEDFN FKLKNGKKFG ISYPCTMLNH LVHAQFTNDQ 950
YHELVNNDSG TYNVKKENSI FFELDGPYKA MILPSSKEED KLLKKRYAVF 1000
NPDGSLAELK GFEVKRRGEL QMIKIFQSQI FDKFLLGKTT EECYAAVATV 1050
ADQWLDILQS KASSLHDDEL VDLIAENRSM SKTLAEYAGQ KSTSISTARR 1100
LAEFLGEQMV KDKGLSCRFI ISAKPNGAPV TERAVPVAIF TAEEPVKRHY 1150
LRKWLKDNSL TDFDLRTILD WDYYTERLGS VIQKLITIPA ALQKVPNPVP 1200
RIRHPDWLYK RIATKEDKFQ QHKITDMFTK LKDMEDLGDG QKKVGPKLAV 1250
VRRRNRKEQE KESEVEEVPP EPEYDYAGYI RIMKKKWRKQ RQEKARARKS 1300
GLRQDGTISS MLRTQTSSMN SKQWDVIQIA STNRPGEFKL WLAIDGTFQS 1350
VRLKVPREFY LNFKEDPEPQ MLATDRYEAV EVVRTLPRGQ PARHLYKLSV 1400
DEVLFMEGES HFSTLINNPN VDGAFELQVP LVVRALLSLG TSCALRSNIL 1450
GGLNRGLDKG FDLVDLERSG NLSRRKYLNE GRGIRYHFLF HAVADQRHII 1500
GLFSPDSTNA SIYLVDRAKN RQQLPNPLKF YTDRVERAER GVFSYPDMLD 1550
FTTSYHSSES SAFKALGKDL QAINHGLNVI ALCSPFEHSY YQAKAPVYSN 1600
FPFITYRLGK EEDPGLMWLL QTSRRMIGLY LRLSSWLKEQ IQIASHFDVP 1650
IGNLGADIAV FLADIEFARR LKQQDMLIWW SSTPRPDLGG SEEDANSSED 1700
LVSPHISNRG CYSSTVLEME VSDLAINAVL QSALVNEMEG SGTGSLAFDS 1750
ASHNLDEYAK GAVNTSVMLG DAVLSTQTFG VLKSMLRAWY ADKARAHVKG 1800
DSLSPAEIVV DQFWRWISSS TSSMFEPALH RFLHGLMRKT FLQLLAEFKR 1850
LGTQVVYADF GRVFLLTSKP DAGSAFAFAK YLVAAANSQE LFRHLIIDVA 1900
QFWNYLAWMD VANFGGVKVS PETAASREPP AKRFEISMDW NIQSFLPGTL 1950
QPVFERNVAS FIFSLYSAKR SSSDGREPLR VIHSLNIDQP GTEATSTMNP 2000
TKEKEKKAAS KSISQTLTRR LLSDIAAVKR QQAAVHLEPE EAYTLAFPDL 2050
PGARSKRINP TLELIKAITE VYSLASEHSI EVQILKRNLL DLIGVKEFSS 2100
DAAFNPPCES IEVPMVICKK CNAIRDVDLC RDPDRLPSVN PDSGEMLEPA 2150
RKNWVCHKCD SEYDRFQIEQ PLIEMVTKTI TNYQIQDVIC LKCSQTKSDN 2200
LAATCKCGGG FRTTSNRNEL KTKLKMIKSV CDYHKLPFAG SYVEETLSRW 2250
Length:2,250
Mass (Da):255,999
Last modified:June 28, 2011 - v1
Checksum:i30EED4407247B80B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017351 Genomic DNA. Translation: AAW46304.1.
RefSeqiXP_567821.1. XM_567821.1.
UniGeneiFne.4301.

Genome annotation databases

EnsemblFungiiAAW46304; AAW46304; CNK01430.
GeneIDi3254656.
KEGGicne:CNK01430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017351 Genomic DNA. Translation: AAW46304.1 .
RefSeqi XP_567821.1. XM_567821.1.
UniGenei Fne.4301.

3D structure databases

ProteinModelPortali P0CN26.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii AAW46304 ; AAW46304 ; CNK01430 .
GeneIDi 3254656.
KEGGi cne:CNK01430.

Phylogenomic databases

KOi K02324.
OrthoDBi EOG790G80.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view ]
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
    Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
    , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
    Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JEC21 / ATCC MYA-565.

Entry informationi

Entry nameiDPOE_CRYNJ
AccessioniPrimary (citable) accession number: P0CN26
Secondary accession number(s): Q55JX3, Q5K9M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi