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Protein

DNA ligase 4

Gene

LIG4

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologous end-joining.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295ATPBy similarity1
Active sitei297N6-AMP-lysine intermediatePROSITE-ProRule annotation1
Binding sitei302ATPBy similarity1
Binding sitei319ATPBy similarity1
Metal bindingi357Magnesium 1Sequence analysis1
Metal bindingi452Magnesium 2Sequence analysis1
Binding sitei457ATPBy similarity1
Binding sitei468ATPBy similarity1
Binding sitei474ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Ordered Locus Names:CNK00930
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000002149 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiFungiDB:CNK00930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002783811 – 1065DNA ligase 4Add BLAST1065

Proteomic databases

PaxDbiP0CN08

Interactioni

Protein-protein interaction databases

STRINGi214684.XP_567656.1

Structurei

3D structure databases

ProteinModelPortaliP0CN08
SMRiP0CN08
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini696 – 775BRCT 1PROSITE-ProRule annotationAdd BLAST80
Domaini954 – 1064BRCT 2PROSITE-ProRule annotationAdd BLAST111

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0966 Eukaryota
COG1793 LUCA
InParanoidiP0CN08
KOiK10777
OMAiGTHIVRM
OrthoDBiEOG092C18KW

Family and domain databases

CDDicd00027 BRCT, 2 hits
Gene3Di1.10.3260.10, 1 hit
3.40.50.10190, 2 hits
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR000977 DNA_ligase_ATP-dep
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR029710 LIG4
IPR012340 NA-bd_OB-fold
PANTHERiPTHR10459:SF7 PTHR10459:SF7, 1 hit
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF16589 BRCT_2, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 2 hits
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 2 hits
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 2 hits
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

P0CN08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVHAPYNHA PPPTQEINGQ KPTLAPAITL ERPADCINRG QYPAFYIICG
60 70 80 90 100
YLNRLRADAP HKKYELLTRI FGNWREKVGP DLYPLIRLLL PDKDRERPVY
110 120 130 140 150
NLKESMLARC YIDILSLEKH SEAAQRLIKW KQPAGNSPNP TGDFAKVCYN
160 170 180 190 200
EIKARSTVEE GQLSVEAVNM LLDKLAVGKM KQKDYVPILK AINMQCTAEE
210 220 230 240 250
QEWIIRIILK DLHISIRERG VLSAFHPDAI DLYNVCSDLK RVCWTLYDPG
260 270 280 290 300
FRLNKNETHL ELFHSFLPQL CGRMNDASLE NIAKAIGAPK EFIMEEKLDG
310 320 330 340 350
ERIQLHMRGN GAQWFYCSRK AKDYTYLYGA HPGEGSLTRY IATAFQDNVR
360 370 380 390 400
NVILDGEMMV WDPVVERYLA FGTLKSAALA FSLVKVFDIL FLNDHCLSRK
410 420 430 440 450
RLSERKRLLR SGKIFKNIEN YKGRLEFVDE KRGKNAKDIR EYLERVVETK
460 470 480 490 500
GEGLVVKKTD VIYQTNSRGY DWIKVKPEYS DQMGENLEVL VLGGWWGKGG
510 520 530 540 550
RSGKISRLLC GLREQAFDDG TLQDFLFNRF WNELLGLRVD FVSHLMHLRS
560 570 580 590 600
MRCSPTHLSQ CRNKHKKHWR PFDRSNPPPF MKLGPVGLDD KPDVYIEPEN
610 620 630 640 650
SFVIEVKASE IVPAGYGIGF TLRFPRCKYI YYDKNSRDYA LDDECLWTVG
660 670 680 690 700
QGFFYKTRKL IKRTDFMDLF SRPKRSYDDS QGPGNFRGQK LSDADVETSI
710 720 730 740 750
FSDMTFSDLE ALVHKHGADF TQAQLSDLSA IVISPDQKNP LVRAQIRHGV
760 770 780 790 800
NVIKPEWVFE SIARRTALPF LKEFLVFASE EAQDGRYYNK TLEQYDKVSF
810 820 830 840 850
VRDRTGGALV DEDGDADVED EIMDGEDKDE IDVEESRESK NRRMAREDLK
860 870 880 890 900
EKESNRTLEQ KKLQEAWGLR SRASPGDSDS EPEEEMSLKE ESDTDSERSR
910 920 930 940 950
GLRAIYEDEE DGENDSHESD VGVNGDDYRA VPLSGLNDKE EGLMGESPEA
960 970 980 990 1000
MHYDEDRIFY HLAFYIDTAK NAAVNGLESS SPSFDTQERL VKVEKLLIEN
1010 1020 1030 1040 1050
GGRVARSISD PKLTHIIMDD EDSRRYVELT RKTAMPKRKH IVTPKWVEDC
1060
VDEETLLDED LYKPK
Length:1,065
Mass (Da):122,466
Last modified:June 28, 2011 - v1
Checksum:i7E0AC640AB2D6236
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017351 Genomic DNA Translation: AAW46139.1
RefSeqiXP_567656.1, XM_567656.1

Genome annotation databases

EnsemblFungiiAAW46139; AAW46139; CNK00930
GeneIDi3254392
KEGGicne:CNK00930

Similar proteinsi

Entry informationi

Entry nameiDNLI4_CRYNJ
AccessioniPrimary (citable) accession number: P0CN08
Secondary accession number(s): Q55JS6, Q5K9S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: March 28, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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