Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CN08

- DNLI4_CRYNJ

UniProt

P0CN08 - DNLI4_CRYNJ

Protein

DNA ligase 4

Gene

LIG4

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei295 – 2951ATPBy similarity
    Active sitei297 – 2971N6-AMP-lysine intermediatePROSITE-ProRule annotation
    Binding sitei302 – 3021ATPBy similarity
    Binding sitei319 – 3191ATPBy similarity
    Metal bindingi357 – 3571Magnesium 1Sequence Analysis
    Metal bindingi452 – 4521Magnesium 2Sequence Analysis
    Binding sitei457 – 4571ATPBy similarity
    Binding sitei468 – 4681ATPBy similarity
    Binding sitei474 – 4741ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA ligation involved in DNA repair Source: InterPro
    2. DNA recombination Source: UniProtKB-KW
    3. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 4 (EC:6.5.1.1)
    Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4
    Gene namesi
    Name:LIG4
    Ordered Locus Names:CNK00930
    OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
    Taxonomic identifieri214684 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000002149: Chromosome 11

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10651065DNA ligase 4PRO_0000278381Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0CN08.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini696 – 77580BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini954 – 1064111BRCT 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated
    Contains 2 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    KOiK10777.
    OrthoDBiEOG7QC84G.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProiIPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF00533. BRCT. 2 hits.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 2 hits.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CN08-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVHAPYNHA PPPTQEINGQ KPTLAPAITL ERPADCINRG QYPAFYIICG     50
    YLNRLRADAP HKKYELLTRI FGNWREKVGP DLYPLIRLLL PDKDRERPVY 100
    NLKESMLARC YIDILSLEKH SEAAQRLIKW KQPAGNSPNP TGDFAKVCYN 150
    EIKARSTVEE GQLSVEAVNM LLDKLAVGKM KQKDYVPILK AINMQCTAEE 200
    QEWIIRIILK DLHISIRERG VLSAFHPDAI DLYNVCSDLK RVCWTLYDPG 250
    FRLNKNETHL ELFHSFLPQL CGRMNDASLE NIAKAIGAPK EFIMEEKLDG 300
    ERIQLHMRGN GAQWFYCSRK AKDYTYLYGA HPGEGSLTRY IATAFQDNVR 350
    NVILDGEMMV WDPVVERYLA FGTLKSAALA FSLVKVFDIL FLNDHCLSRK 400
    RLSERKRLLR SGKIFKNIEN YKGRLEFVDE KRGKNAKDIR EYLERVVETK 450
    GEGLVVKKTD VIYQTNSRGY DWIKVKPEYS DQMGENLEVL VLGGWWGKGG 500
    RSGKISRLLC GLREQAFDDG TLQDFLFNRF WNELLGLRVD FVSHLMHLRS 550
    MRCSPTHLSQ CRNKHKKHWR PFDRSNPPPF MKLGPVGLDD KPDVYIEPEN 600
    SFVIEVKASE IVPAGYGIGF TLRFPRCKYI YYDKNSRDYA LDDECLWTVG 650
    QGFFYKTRKL IKRTDFMDLF SRPKRSYDDS QGPGNFRGQK LSDADVETSI 700
    FSDMTFSDLE ALVHKHGADF TQAQLSDLSA IVISPDQKNP LVRAQIRHGV 750
    NVIKPEWVFE SIARRTALPF LKEFLVFASE EAQDGRYYNK TLEQYDKVSF 800
    VRDRTGGALV DEDGDADVED EIMDGEDKDE IDVEESRESK NRRMAREDLK 850
    EKESNRTLEQ KKLQEAWGLR SRASPGDSDS EPEEEMSLKE ESDTDSERSR 900
    GLRAIYEDEE DGENDSHESD VGVNGDDYRA VPLSGLNDKE EGLMGESPEA 950
    MHYDEDRIFY HLAFYIDTAK NAAVNGLESS SPSFDTQERL VKVEKLLIEN 1000
    GGRVARSISD PKLTHIIMDD EDSRRYVELT RKTAMPKRKH IVTPKWVEDC 1050
    VDEETLLDED LYKPK 1065
    Length:1,065
    Mass (Da):122,466
    Last modified:June 28, 2011 - v1
    Checksum:i7E0AC640AB2D6236
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017351 Genomic DNA. Translation: AAW46139.1.
    RefSeqiXP_567656.1. XM_567656.1.

    Genome annotation databases

    EnsemblFungiiAAW46139; AAW46139; CNK00930.
    GeneIDi3254392.
    KEGGicne:CNK00930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017351 Genomic DNA. Translation: AAW46139.1 .
    RefSeqi XP_567656.1. XM_567656.1.

    3D structure databases

    ProteinModelPortali P0CN08.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii AAW46139 ; AAW46139 ; CNK00930 .
    GeneIDi 3254392.
    KEGGi cne:CNK00930.

    Phylogenomic databases

    KOi K10777.
    OrthoDBi EOG7QC84G.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProi IPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF00533. BRCT. 2 hits.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
      Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
      , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
      Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JEC21 / ATCC MYA-565.

    Entry informationi

    Entry nameiDNLI4_CRYNJ
    AccessioniPrimary (citable) accession number: P0CN08
    Secondary accession number(s): Q55JS6, Q5K9S5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3