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P0CN08 (DNLI4_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:LIG4
Ordered Locus Names:CNK00930
OrganismCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome]
Taxonomic identifier214684 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length1065 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10651065DNA ligase 4
PRO_0000278381

Regions

Domain696 – 77580BRCT 1
Domain954 – 1064111BRCT 2

Sites

Active site2971N6-AMP-lysine intermediate By similarity
Metal binding3571Magnesium 1 Potential
Metal binding4521Magnesium 2 Potential
Binding site2951ATP By similarity
Binding site3021ATP By similarity
Binding site3191ATP By similarity
Binding site4571ATP By similarity
Binding site4681ATP By similarity
Binding site4741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0CN08 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 7E0AC640AB2D6236

FASTA1,065122,466
        10         20         30         40         50         60 
MAVHAPYNHA PPPTQEINGQ KPTLAPAITL ERPADCINRG QYPAFYIICG YLNRLRADAP 

        70         80         90        100        110        120 
HKKYELLTRI FGNWREKVGP DLYPLIRLLL PDKDRERPVY NLKESMLARC YIDILSLEKH 

       130        140        150        160        170        180 
SEAAQRLIKW KQPAGNSPNP TGDFAKVCYN EIKARSTVEE GQLSVEAVNM LLDKLAVGKM 

       190        200        210        220        230        240 
KQKDYVPILK AINMQCTAEE QEWIIRIILK DLHISIRERG VLSAFHPDAI DLYNVCSDLK 

       250        260        270        280        290        300 
RVCWTLYDPG FRLNKNETHL ELFHSFLPQL CGRMNDASLE NIAKAIGAPK EFIMEEKLDG 

       310        320        330        340        350        360 
ERIQLHMRGN GAQWFYCSRK AKDYTYLYGA HPGEGSLTRY IATAFQDNVR NVILDGEMMV 

       370        380        390        400        410        420 
WDPVVERYLA FGTLKSAALA FSLVKVFDIL FLNDHCLSRK RLSERKRLLR SGKIFKNIEN 

       430        440        450        460        470        480 
YKGRLEFVDE KRGKNAKDIR EYLERVVETK GEGLVVKKTD VIYQTNSRGY DWIKVKPEYS 

       490        500        510        520        530        540 
DQMGENLEVL VLGGWWGKGG RSGKISRLLC GLREQAFDDG TLQDFLFNRF WNELLGLRVD 

       550        560        570        580        590        600 
FVSHLMHLRS MRCSPTHLSQ CRNKHKKHWR PFDRSNPPPF MKLGPVGLDD KPDVYIEPEN 

       610        620        630        640        650        660 
SFVIEVKASE IVPAGYGIGF TLRFPRCKYI YYDKNSRDYA LDDECLWTVG QGFFYKTRKL 

       670        680        690        700        710        720 
IKRTDFMDLF SRPKRSYDDS QGPGNFRGQK LSDADVETSI FSDMTFSDLE ALVHKHGADF 

       730        740        750        760        770        780 
TQAQLSDLSA IVISPDQKNP LVRAQIRHGV NVIKPEWVFE SIARRTALPF LKEFLVFASE 

       790        800        810        820        830        840 
EAQDGRYYNK TLEQYDKVSF VRDRTGGALV DEDGDADVED EIMDGEDKDE IDVEESRESK 

       850        860        870        880        890        900 
NRRMAREDLK EKESNRTLEQ KKLQEAWGLR SRASPGDSDS EPEEEMSLKE ESDTDSERSR 

       910        920        930        940        950        960 
GLRAIYEDEE DGENDSHESD VGVNGDDYRA VPLSGLNDKE EGLMGESPEA MHYDEDRIFY 

       970        980        990       1000       1010       1020 
HLAFYIDTAK NAAVNGLESS SPSFDTQERL VKVEKLLIEN GGRVARSISD PKLTHIIMDD 

      1030       1040       1050       1060 
EDSRRYVELT RKTAMPKRKH IVTPKWVEDC VDEETLLDED LYKPK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017351 Genomic DNA. Translation: AAW46139.1.
RefSeqXP_567656.1. XM_567656.1.

3D structure databases

ProteinModelPortalP0CN08.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAW46139; AAW46139; CNK00930.
GeneID3254392.
KEGGcne:CNK00930.

Phylogenomic databases

KOK10777.
OrthoDBEOG7QC84G.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_CRYNJ
AccessionPrimary (citable) accession number: P0CN08
Secondary accession number(s): Q55JS6, Q5K9S5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 13, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families