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P0CN08

- DNLI4_CRYNJ

UniProt

P0CN08 - DNLI4_CRYNJ

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Protein

DNA ligase 4

Gene

LIG4

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (By similarity).By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei295 – 2951ATPBy similarity
Active sitei297 – 2971N6-AMP-lysine intermediatePROSITE-ProRule annotation
Binding sitei302 – 3021ATPBy similarity
Binding sitei319 – 3191ATPBy similarity
Metal bindingi357 – 3571Magnesium 1Sequence Analysis
Metal bindingi452 – 4521Magnesium 2Sequence Analysis
Binding sitei457 – 4571ATPBy similarity
Binding sitei468 – 4681ATPBy similarity
Binding sitei474 – 4741ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA biosynthetic process Source: InterPro
  2. DNA ligation involved in DNA repair Source: InterPro
  3. DNA recombination Source: UniProtKB-KW
  4. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Ordered Locus Names:CNK00930
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000002149: Chromosome 11

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10651065DNA ligase 4PRO_0000278381Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP0CN08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini696 – 77580BRCT 1PROSITE-ProRule annotationAdd
BLAST
Domaini954 – 1064111BRCT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiP0CN08.
KOiK10777.
OrthoDBiEOG7QC84G.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 1 hit.
PfamiPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CN08-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVHAPYNHA PPPTQEINGQ KPTLAPAITL ERPADCINRG QYPAFYIICG
60 70 80 90 100
YLNRLRADAP HKKYELLTRI FGNWREKVGP DLYPLIRLLL PDKDRERPVY
110 120 130 140 150
NLKESMLARC YIDILSLEKH SEAAQRLIKW KQPAGNSPNP TGDFAKVCYN
160 170 180 190 200
EIKARSTVEE GQLSVEAVNM LLDKLAVGKM KQKDYVPILK AINMQCTAEE
210 220 230 240 250
QEWIIRIILK DLHISIRERG VLSAFHPDAI DLYNVCSDLK RVCWTLYDPG
260 270 280 290 300
FRLNKNETHL ELFHSFLPQL CGRMNDASLE NIAKAIGAPK EFIMEEKLDG
310 320 330 340 350
ERIQLHMRGN GAQWFYCSRK AKDYTYLYGA HPGEGSLTRY IATAFQDNVR
360 370 380 390 400
NVILDGEMMV WDPVVERYLA FGTLKSAALA FSLVKVFDIL FLNDHCLSRK
410 420 430 440 450
RLSERKRLLR SGKIFKNIEN YKGRLEFVDE KRGKNAKDIR EYLERVVETK
460 470 480 490 500
GEGLVVKKTD VIYQTNSRGY DWIKVKPEYS DQMGENLEVL VLGGWWGKGG
510 520 530 540 550
RSGKISRLLC GLREQAFDDG TLQDFLFNRF WNELLGLRVD FVSHLMHLRS
560 570 580 590 600
MRCSPTHLSQ CRNKHKKHWR PFDRSNPPPF MKLGPVGLDD KPDVYIEPEN
610 620 630 640 650
SFVIEVKASE IVPAGYGIGF TLRFPRCKYI YYDKNSRDYA LDDECLWTVG
660 670 680 690 700
QGFFYKTRKL IKRTDFMDLF SRPKRSYDDS QGPGNFRGQK LSDADVETSI
710 720 730 740 750
FSDMTFSDLE ALVHKHGADF TQAQLSDLSA IVISPDQKNP LVRAQIRHGV
760 770 780 790 800
NVIKPEWVFE SIARRTALPF LKEFLVFASE EAQDGRYYNK TLEQYDKVSF
810 820 830 840 850
VRDRTGGALV DEDGDADVED EIMDGEDKDE IDVEESRESK NRRMAREDLK
860 870 880 890 900
EKESNRTLEQ KKLQEAWGLR SRASPGDSDS EPEEEMSLKE ESDTDSERSR
910 920 930 940 950
GLRAIYEDEE DGENDSHESD VGVNGDDYRA VPLSGLNDKE EGLMGESPEA
960 970 980 990 1000
MHYDEDRIFY HLAFYIDTAK NAAVNGLESS SPSFDTQERL VKVEKLLIEN
1010 1020 1030 1040 1050
GGRVARSISD PKLTHIIMDD EDSRRYVELT RKTAMPKRKH IVTPKWVEDC
1060
VDEETLLDED LYKPK
Length:1,065
Mass (Da):122,466
Last modified:June 28, 2011 - v1
Checksum:i7E0AC640AB2D6236
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017351 Genomic DNA. Translation: AAW46139.1.
RefSeqiXP_567656.1. XM_567656.1.

Genome annotation databases

EnsemblFungiiAAW46139; AAW46139; CNK00930.
GeneIDi3254392.
KEGGicne:CNK00930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017351 Genomic DNA. Translation: AAW46139.1 .
RefSeqi XP_567656.1. XM_567656.1.

3D structure databases

ProteinModelPortali P0CN08.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii AAW46139 ; AAW46139 ; CNK00930 .
GeneIDi 3254392.
KEGGi cne:CNK00930.

Phylogenomic databases

InParanoidi P0CN08.
KOi K10777.
OrthoDBi EOG7QC84G.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
PANTHERi PTHR10459:SF7. PTHR10459:SF7. 1 hit.
Pfami PF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
    Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
    , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
    Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JEC21 / ATCC MYA-565.

Entry informationi

Entry nameiDNLI4_CRYNJ
AccessioniPrimary (citable) accession number: P0CN08
Secondary accession number(s): Q55JS6, Q5K9S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 26, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3