P0CL98 (ACKA_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetate kinase
EC=2.7.2.1

Alternative name(s):
Acetokinase

Gene names

Ordered Locus Names:

CNN01560

Organism

Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome]

Taxonomic identifier

214684 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex › Cryptococcus neoformans › Cryptococcus neoformans var. neoformans

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP-Rule MF_03131
Cofactor	Mg2+ By similarity.
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP-Rule MF_03131
Sequence similarities	Belongs to the acetokinase family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway organic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Probable acetate kinase HAMAP-Rule MF_03131		PRO_0000402093
Regions
	Nucleotide binding	220 – 224	5	ATP By similarity
Sites
	Active site	159	1	Proton donor/acceptor By similarity
	Metal binding	12	1	Magnesium By similarity
	Metal binding	416	1	Magnesium By similarity
	Binding site	19	1	ATP By similarity
	Binding site	100	1	Substrate By similarity
	Site	192	1	Transition state stabilizer By similarity
	Site	253	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0CL98 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 21519B732FAB7114	FASTA	430	46,972
	10 20 30 40 50 60 MPDKTEYLLA INCGSSSIKG KLFAIPSFEL LANLAVTNIS SADERVKIRI TWEEGKGKNS 70 80 90 100 110 120 EEEADYGDKI RYASLVPILL DHLTNSTHVE KEEIKYVCHR VVHGGTHDRG IRVVKGHEEG 130 140 150 160 170 180 LIEMDKLSEF APLHNHRAVL AVKSCLDALP HHTSLLLFDT IFHQTIAPEV YTYALPPSDN 190 200 210 220 230 240 ELSMPLRKYG FHGLSYASIV RSLAEHLKKP SDQVNVVVAH LGSGSSSCCI KNGKSVDTSM 250 260 270 280 290 300 GLTPLEGLLG GTRSGTIDPT AIFHHTKDAA SDANVGDFTV SKAEIILNKN SGLKALAGTT 310 320 330 340 350 360 NFGHIIQNLD PSKCSKEDHE KAKLTYAVFL DRLLNFVAQY LFKLLSEVPI ESIDGLVFSG 370 380 390 400 410 420 GIGEKGAELR RDVLKKLAWL GAEVDEEANN SNSGGTVKCI TKEGSKLKGW VVETDEEGWM 430 ATMAKEEFGF

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JEC21 / ATCC MYA-565.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017356 Genomic DNA. Translation: AAW47117.1.
RefSeq	XP_568634.1. XM_568634.1.
UniGene	Fne.6851.
3D structure databases
ProteinModelPortal	P0CL98.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3255391.
KEGG	cne:CNN01560.
Phylogenomic databases
KO	K00925.
Enzyme and pathway databases
UniPathway	UPA00340; UER00458.
Family and domain databases
HAMAP	MF_00020. Acetate_kinase.
InterPro	IPR004372. Ac/Proprionate_kinase. IPR000890. Aliphatic_acid_kin_short-chain. IPR023865. Aliphatic_acid_kinase_CS. [Graphical view]
PANTHER	PTHR21060. PTHR21060. 1 hit.
Pfam	PF00871. Acetate_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTS	PR00471. ACETATEKNASE.
TIGRFAMs	TIGR00016. ackA. 1 hit.
PROSITE	PS01075. ACETATE_KINASE_1. 1 hit. PS01076. ACETATE_KINASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACKA_CRYNJ

Accession

Primary (citable) accession number: P0CL98
Secondary accession number(s): Q55HG8, Q5K706

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program