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P0CL75 (DNLI_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:PYRAB05430
ORF Names:PAB2002
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559DNA ligase HAMAP-Rule MF_00407
PRO_0000407282

Sites

Active site2491N6-AMP-lysine intermediate By similarity
Binding site2471ATP By similarity
Binding site2541ATP By similarity
Binding site2691ATP By similarity
Binding site2991ATP By similarity
Binding site3391ATP By similarity
Binding site4141ATP By similarity
Binding site4201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0CL75 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 64CDBA1BBD188433

FASTA55963,553
        10         20         30         40         50         60 
MRYIELAQLY QKLEKTTMKL IKTRLVADFL KKVPEDHLEF IPYLILGDVF PEWDERELGV 

        70         80         90        100        110        120 
GEKLLIKAVS MATGIDSKEI ENSVKDTGDL GESIALAVKK RKQKSFFSQP LTIKRVYQTL 

       130        140        150        160        170        180 
VKVAETTGEG SQDKKMKYLA NLFMDAEPIE AKYIARTVLG TMRTGVAEGL LRDAISLAFN 

       190        200        210        220        230        240 
VKVELVERAY MLTSDFGFVA KIAKTEGNDG LAKVTIQIGK PIKPMLAQQA ANIKEALLEM 

       250        260        270        280        290        300 
GGEAEFEIKY DGARVQVHKD GEKVTIYSRR LENVTRAIPE IVEAIKEALK PTKAIVEGEL 

       310        320        330        340        350        360 
VAIGEDGRPL PFQYVLRRFR RKYNIEEMME KIPLELNLFD VLYVDGVSLI DTKFMERRKK 

       370        380        390        400        410        420 
LEEIVETNGK VKIAENLITK NVEEAEQFYK RALEMGHEGL MAKRLDAVYE PGNRGKKWLK 

       430        440        450        460        470        480 
IKPTMENLDL VIIGAEWGEG RRAHLLGSFI LGAYDPETGE FLEVGKVGSG FTDDDLVEFT 

       490        500        510        520        530        540 
KMLKPLIIKE EGKRVWIQPK VVIEVTYQEI QKSPKYRSGF ALRFPRYVAL REDKGPEDAD 

       550 
TIERIAQLYE LQERMKGKV 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248284 Genomic DNA. Translation: CAB49465.1.
HE613800 Genomic DNA. Translation: CCE69932.1.
PIRB75173.
RefSeqNP_126234.1. NC_000868.1.

3D structure databases

ProteinModelPortalP0CL75.
SMRP0CL75. Positions 1-559.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB49465; CAB49465; PAB2002.
GeneID1495445.
KEGGpab:PAB2002.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_PYRAB
AccessionPrimary (citable) accession number: P0CL75
Secondary accession number(s): G8ZGQ3, Q9HH10, Q9V185
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 5, 2011
Last modified: February 19, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families