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P0CL75

- DNLI_PYRAB

UniProt

P0CL75 - DNLI_PYRAB

Protein

DNA ligase

Gene

lig

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 24 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Cofactori

    Divalent metal cations.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei247 – 2471ATPBy similarity
    Active sitei249 – 2491N6-AMP-lysine intermediateBy similarity
    Binding sitei254 – 2541ATPBy similarity
    Binding sitei269 – 2691ATPBy similarity
    Binding sitei299 – 2991ATPBy similarity
    Binding sitei339 – 3391ATPBy similarity
    Binding sitei414 – 4141ATPBy similarity
    Binding sitei420 – 4201ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation involved in DNA repair Source: InterPro
    4. DNA recombination Source: UniProtKB-HAMAP
    5. DNA replication Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase (EC:6.5.1.1)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
    Gene namesi
    Name:lig
    Ordered Locus Names:PYRAB05430
    ORF Names:PAB2002
    OrganismiPyrococcus abyssi (strain GE5 / Orsay)
    Taxonomic identifieri272844 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000810: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 559559DNA ligasePRO_0000407282Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0CL75.
    SMRiP0CL75. Positions 1-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000036008.
    KOiK10747.
    OMAiARVQVHK.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CL75-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRYIELAQLY QKLEKTTMKL IKTRLVADFL KKVPEDHLEF IPYLILGDVF    50
    PEWDERELGV GEKLLIKAVS MATGIDSKEI ENSVKDTGDL GESIALAVKK 100
    RKQKSFFSQP LTIKRVYQTL VKVAETTGEG SQDKKMKYLA NLFMDAEPIE 150
    AKYIARTVLG TMRTGVAEGL LRDAISLAFN VKVELVERAY MLTSDFGFVA 200
    KIAKTEGNDG LAKVTIQIGK PIKPMLAQQA ANIKEALLEM GGEAEFEIKY 250
    DGARVQVHKD GEKVTIYSRR LENVTRAIPE IVEAIKEALK PTKAIVEGEL 300
    VAIGEDGRPL PFQYVLRRFR RKYNIEEMME KIPLELNLFD VLYVDGVSLI 350
    DTKFMERRKK LEEIVETNGK VKIAENLITK NVEEAEQFYK RALEMGHEGL 400
    MAKRLDAVYE PGNRGKKWLK IKPTMENLDL VIIGAEWGEG RRAHLLGSFI 450
    LGAYDPETGE FLEVGKVGSG FTDDDLVEFT KMLKPLIIKE EGKRVWIQPK 500
    VVIEVTYQEI QKSPKYRSGF ALRFPRYVAL REDKGPEDAD TIERIAQLYE 550
    LQERMKGKV 559
    Length:559
    Mass (Da):63,553
    Last modified:April 5, 2011 - v1
    Checksum:i64CDBA1BBD188433
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248284 Genomic DNA. Translation: CAB49465.1.
    HE613800 Genomic DNA. Translation: CCE69932.1.
    PIRiB75173.
    RefSeqiNP_126234.1. NC_000868.1.
    WP_010867667.1. NC_000868.1.

    Genome annotation databases

    EnsemblBacteriaiCAB49465; CAB49465; PAB2002.
    GeneIDi1495445.
    KEGGipab:PAB2002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248284 Genomic DNA. Translation: CAB49465.1 .
    HE613800 Genomic DNA. Translation: CCE69932.1 .
    PIRi B75173.
    RefSeqi NP_126234.1. NC_000868.1.
    WP_010867667.1. NC_000868.1.

    3D structure databases

    ProteinModelPortali P0CL75.
    SMRi P0CL75. Positions 1-559.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB49465 ; CAB49465 ; PAB2002 .
    GeneIDi 1495445.
    KEGGi pab:PAB2002.

    Phylogenomic databases

    HOGENOMi HOG000036008.
    KOi K10747.
    OMAi ARVQVHK.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GE5 / Orsay.
    2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
      Gao J., Wang J.
      Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: GE5 / Orsay.

    Entry informationi

    Entry nameiDNLI_PYRAB
    AccessioniPrimary (citable) accession number: P0CL75
    Secondary accession number(s): G8ZGQ3, Q9HH10, Q9V185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 24 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3