Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CL71

- RNCL_ASPCL

UniProt

P0CL71 - RNCL_ASPCL

Protein

Ribonuclease clavin

Gene

cla

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 19 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Clavin has the same substrate specificity as alpha-sarcin. It is specific for purines in both single- and double-stranded RNA. Its toxic action on eukaryotic cells is the result of cleavage of a single phosphodiester bond in the 60S subunit of ribosomes.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771By similarity
    Active sitei123 – 1231Proton acceptorBy similarity
    Active sitei164 – 1641Proton donorBy similarity

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. negative regulation of translation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Nuclease, Protein synthesis inhibitor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease clavin (EC:3.1.27.-)
    Gene namesi
    Name:cla
    Synonyms:c-sar
    ORF Names:ACLA_002920
    OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
    Taxonomic identifieri344612 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006701: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727By similarityAdd
    BLAST
    Chaini28 – 177150Ribonuclease clavinPRO_0000406983Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 175By similarity
    Disulfide bondi103 ↔ 159By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP0CL71.
    SMRiP0CL71. Positions 28-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease U2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000216956.
    KOiK16639.
    OMAiSSYPHWF.
    OrthoDBiEOG7HHX4D.

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR004025. Fun_ribotoxin.
    IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037430. RNase_U2. 1 hit.
    PRINTSiPR01704. FUNRIBOTOXIN.
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CL71-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAIKNLVLV ALTAVTALAM PSPLEERAAT WTCMNEQKNP KTNKYENKRL    50
    LYNQNNAESN AHHAPLSDGK TGSSYPHWFT NGYDGDGKIL KGRTPIKWGN 100
    SDCDRPPKHS KNGDGKNDHY LLEFPTFPDG HQYNFDSKKP KEDPGPARVI 150
    YTYPNKVFCG IVAHTRENQG DLKLCSH 177
    Length:177
    Mass (Da):19,855
    Last modified:April 5, 2011 - v1
    Checksum:iEA602B8555D7022D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027004 Genomic DNA. Translation: EAW14881.1.
    RefSeqiXP_001276307.1. XM_001276306.1.

    Genome annotation databases

    EnsemblFungiiCADACLAT00000062; CADACLAP00000058; CADACLAG00000062.
    GeneIDi4708501.
    KEGGiact:ACLA_002920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027004 Genomic DNA. Translation: EAW14881.1 .
    RefSeqi XP_001276307.1. XM_001276306.1.

    3D structure databases

    ProteinModelPortali P0CL71.
    SMRi P0CL71. Positions 28-177.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADACLAT00000062 ; CADACLAP00000058 ; CADACLAG00000062 .
    GeneIDi 4708501.
    KEGGi act:ACLA_002920.

    Phylogenomic databases

    HOGENOMi HOG000216956.
    KOi K16639.
    OMAi SSYPHWF.
    OrthoDBi EOG7HHX4D.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR004025. Fun_ribotoxin.
    IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037430. RNase_U2. 1 hit.
    PRINTSi PR01704. FUNRIBOTOXIN.
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.
    2. "Clavin, a type-1 ribosome-inactivating protein from Aspergillus clavatus IFO 8605. cDNA isolation, heterologous expression, biochemical and biological characterization of the recombinant protein."
      Parente D., Raucci G., Celano B., Pacilli A., Zanoni L., Canevari S., Adobati E., Colnaghi M.I., Dosio F., Arpicco S., Cattel L., Mele A., de Santis R.
      Eur. J. Biochem. 239:272-280(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: NBRC 8605.
    3. "Characterization of a new ribotoxin gene (c-sar) from Aspergillus clavatus."
      Huang K.-C., Hwang Y.-Y., Hwu L., Lin A.
      Toxicon 35:383-392(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: BCRC 32114.

    Entry informationi

    Entry nameiRNCL_ASPCL
    AccessioniPrimary (citable) accession number: P0CL71
    Secondary accession number(s): A1C5B3, P49074, P78572
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 19 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3