P0CL71 (RNCL_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease clavin
EC=3.1.27.-

Gene names

Name:	cla
Synonyms:	c-sar
ORF Names:	ACLA_002920

Organism

Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]

Taxonomic identifier

344612 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus clavatus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Clavin has the same substrate-specificity than alpha-sarcin. It is specific for purines in both single- and double-stranded RNA. Its toxic action on eukaryotic cells is the result of cleavage of a single phosphodiester bond in the 60S subunit of ribosomes. Ref.2 Ref.3
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the ribonuclease U2 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Nuclease Protein synthesis inhibitor
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 177	150	Ribonuclease clavin		PRO_0000406983
Sites
	Active site	77	1	By similarity
	Active site	123	1	Proton acceptor By similarity
	Active site	164	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	33 ↔ 175		By similarity
	Disulfide bond	103 ↔ 159		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0CL71 [UniParc]. Last modified April 5, 2011. Version 1. Checksum: EA602B8555D7022D	FASTA	177	19,855
	10 20 30 40 50 60 MVAIKNLVLV ALTAVTALAM PSPLEERAAT WTCMNEQKNP KTNKYENKRL LYNQNNAESN 70 80 90 100 110 120 AHHAPLSDGK TGSSYPHWFT NGYDGDGKIL KGRTPIKWGN SDCDRPPKHS KNGDGKNDHY 130 140 150 160 170 LLEFPTFPDG HQYNFDSKKP KEDPGPARVI YTYPNKVFCG IVAHTRENQG DLKLCSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.
[2]	"Clavin, a type-1 ribosome-inactivating protein from Aspergillus clavatus IFO 8605. cDNA isolation, heterologous expression, biochemical and biological characterization of the recombinant protein." Parente D., Raucci G., Celano B., Pacilli A., Zanoni L., Canevari S., Adobati E., Colnaghi M.I., Dosio F., Arpicco S., Cattel L., Mele A., de Santis R. Eur. J. Biochem. 239:272-280(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: NBRC 8605.
[3]	"Characterization of a new ribotoxin gene (c-sar) from Aspergillus clavatus." Huang K.-C., Hwang Y.-Y., Hwu L., Lin A. Toxicon 35:383-392(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: BCRC 32114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027004 Genomic DNA. Translation: EAW14881.1.
RefSeq	XP_001276307.1. XM_001276306.1.
3D structure databases
ProteinModelPortal	P0CL71.
SMR	P0CL71. Positions 28-177.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4708501.
KEGG	act:ACLA_002920.
Phylogenomic databases
HOGENOM	HOG000216956.
KO	K16639.
Family and domain databases
Gene3D	3.10.450.30. 1 hit.
InterPro	IPR004025. Fun_ribotoxin. IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
PIRSF	PIRSF037430. RNase_U2. 1 hit.
PRINTS	PR01704. FUNRIBOTOXIN.
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNCL_ASPCL

Accession

Primary (citable) accession number: P0CL71
Secondary accession number(s): A1C5B3, P49074, P78572

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	April 5, 2011
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program