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Protein

Ribonuclease clavin

Gene

cla

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Clavin has the same substrate specificity as alpha-sarcin. It is specific for purines in both single- and double-stranded RNA. Its toxic action on eukaryotic cells is the result of cleavage of a single phosphodiester bond in the 60S subunit of ribosomes.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771By similarity
Active sitei123 – 1231Proton acceptorBy similarity
Active sitei164 – 1641Proton donorBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. RNA binding Source: InterPro

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease, Protein synthesis inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease clavin (EC:3.1.27.-)
Gene namesi
Name:cla
Synonyms:c-sar
ORF Names:ACLA_002920
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006701: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 177150Ribonuclease clavinPRO_0000406983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 175By similarity
Disulfide bondi103 ↔ 159By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP0CL71.
SMRiP0CL71. Positions 28-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease U2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000216956.
KOiK16639.
OMAiSSYPHWF.
OrthoDBiEOG7HHX4D.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CL71-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAIKNLVLV ALTAVTALAM PSPLEERAAT WTCMNEQKNP KTNKYENKRL
60 70 80 90 100
LYNQNNAESN AHHAPLSDGK TGSSYPHWFT NGYDGDGKIL KGRTPIKWGN
110 120 130 140 150
SDCDRPPKHS KNGDGKNDHY LLEFPTFPDG HQYNFDSKKP KEDPGPARVI
160 170
YTYPNKVFCG IVAHTRENQG DLKLCSH
Length:177
Mass (Da):19,855
Last modified:April 5, 2011 - v1
Checksum:iEA602B8555D7022D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027004 Genomic DNA. Translation: EAW14881.1.
RefSeqiXP_001276307.1. XM_001276306.1.

Genome annotation databases

EnsemblFungiiCADACLAT00000062; CADACLAP00000058; CADACLAG00000062.
GeneIDi4708501.
KEGGiact:ACLA_002920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027004 Genomic DNA. Translation: EAW14881.1.
RefSeqiXP_001276307.1. XM_001276306.1.

3D structure databases

ProteinModelPortaliP0CL71.
SMRiP0CL71. Positions 28-177.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADACLAT00000062; CADACLAP00000058; CADACLAG00000062.
GeneIDi4708501.
KEGGiact:ACLA_002920.

Phylogenomic databases

HOGENOMiHOG000216956.
KOiK16639.
OMAiSSYPHWF.
OrthoDBiEOG7HHX4D.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.
  2. "Clavin, a type-1 ribosome-inactivating protein from Aspergillus clavatus IFO 8605. cDNA isolation, heterologous expression, biochemical and biological characterization of the recombinant protein."
    Parente D., Raucci G., Celano B., Pacilli A., Zanoni L., Canevari S., Adobati E., Colnaghi M.I., Dosio F., Arpicco S., Cattel L., Mele A., de Santis R.
    Eur. J. Biochem. 239:272-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: NBRC 8605.
  3. "Characterization of a new ribotoxin gene (c-sar) from Aspergillus clavatus."
    Huang K.-C., Hwang Y.-Y., Hwu L., Lin A.
    Toxicon 35:383-392(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: BCRC 32114.

Entry informationi

Entry nameiRNCL_ASPCL
AccessioniPrimary (citable) accession number: P0CL71
Secondary accession number(s): A1C5B3, P49074, P78572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 5, 2011
Last modified: January 7, 2015
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.