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P0CL70

- RNCL_ASPCV

UniProt

P0CL70 - RNCL_ASPCV

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Protein

Ribonuclease clavin

Gene

cla

Organism
Aspergillus clavatus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Clavin has the same substrate specificity as alpha-sarcin. It is specific for purines in both single- and double-stranded RNA. Its toxic action on eukaryotic cells is the result of cleavage of a single phosphodiester bond in the 60S subunit of ribosomes.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771By similarity
Active sitei123 – 1231Proton acceptorBy similarity
Active sitei164 – 1641Proton donorBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. RNA binding Source: InterPro

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease, Protein synthesis inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease clavin (EC:3.1.27.-)
Gene namesi
Name:cla
Synonyms:c-sar
OrganismiAspergillus clavatus
Taxonomic identifieri5057 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 177150Ribonuclease clavinPRO_0000030835Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 175By similarity
Disulfide bondi103 ↔ 159By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP0CL70.
SMRiP0CL70. Positions 28-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease U2 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CL70-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAIKNLVLV ALTAVTALAM PSPLEERAAT WTCMNEQKNP KTNKYENKRL
60 70 80 90 100
LYNQNNAESN AHHAPLSDGK TGSSYPHWFT NGYDGDGKIL KGRTPIKWGN
110 120 130 140 150
SDCDRPPKHS KNGDGKNDHY LLEFPTFPDG HQYNFDSKKP KEDPGPARVI
160 170
YTYPNKVFCG IVAHTRENQG DLKLCSH
Length:177
Mass (Da):19,855
Last modified:April 5, 2011 - v1
Checksum:iEA602B8555D7022D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131T → M in AAB42201. (PubMed:9080594)Curated
Sequence conflicti98 – 981W → L in AAB42201. (PubMed:9080594)Curated
Sequence conflicti113 – 1131G → V in AAB42201. (PubMed:9080594)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19383 mRNA. Translation: AAC49407.1.
U48731 Genomic DNA. Translation: AAB42201.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19383 mRNA. Translation: AAC49407.1 .
U48731 Genomic DNA. Translation: AAB42201.1 .

3D structure databases

ProteinModelPortali P0CL70.
SMRi P0CL70. Positions 28-177.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
PIRSFi PIRSF037430. RNase_U2. 1 hit.
PRINTSi PR01704. FUNRIBOTOXIN.
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Clavin, a type-1 ribosome-inactivating protein from Aspergillus clavatus IFO 8605. cDNA isolation, heterologous expression, biochemical and biological characterization of the recombinant protein."
    Parente D., Raucci G., Celano B., Pacilli A., Zanoni L., Canevari S., Adobati E., Colnaghi M.I., Dosio F., Arpicco S., Cattel L., Mele A., de Santis R.
    Eur. J. Biochem. 239:272-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: NBRC 8605.
  2. "Characterization of a new ribotoxin gene (c-sar) from Aspergillus clavatus."
    Huang K.-C., Hwang Y.-Y., Hwu L., Lin A.
    Toxicon 35:383-392(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BCRC 32114.

Entry informationi

Entry nameiRNCL_ASPCV
AccessioniPrimary (citable) accession number: P0CL70
Secondary accession number(s): A1C5B3, P49074, P78572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 5, 2011
Last modified: October 29, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3