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P0CL66

- OSPA_BORBU

UniProt

P0CL66 - OSPA_BORBU

Protein

Outer surface protein A

Gene

ospA

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    Enzyme and pathway databases

    BioCyciBBUR224326:G9SO-876-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer surface protein A
    Gene namesi
    Name:ospA
    Ordered Locus Names:BB_A15
    Encoded oniPlasmid lp540 Publication
    OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
    Taxonomic identifieri224326 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001807: Plasmid lp54

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Add
    BLAST
    Chaini17 – 273257Outer surface protein APRO_0000018074Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi17 – 171N-palmitoyl cysteineCurated
    Lipidationi17 – 171S-diacylglycerol cysteineCurated

    Keywords - PTMi

    Lipoprotein, Palmitate

    Structurei

    Secondary structure

    1
    273
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi26 – 283
    Beta strandi30 – 345
    Turni35 – 373
    Beta strandi38 – 425
    Beta strandi48 – 503
    Beta strandi52 – 587
    Beta strandi61 – 7212
    Beta strandi74 – 796
    Beta strandi85 – 906
    Beta strandi97 – 1026
    Beta strandi108 – 1158
    Turni117 – 1193
    Beta strandi121 – 1266
    Beta strandi132 – 1387
    Beta strandi144 – 1496
    Beta strandi152 – 1543
    Beta strandi156 – 1627
    Beta strandi165 – 1717
    Beta strandi173 – 18210
    Beta strandi185 – 1928
    Beta strandi197 – 2037
    Turni208 – 2103
    Beta strandi212 – 2176
    Turni218 – 2214
    Beta strandi222 – 2276
    Beta strandi230 – 2378
    Beta strandi243 – 2486
    Beta strandi252 – 2554
    Beta strandi260 – 2623
    Helixi265 – 2717

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FJ1X-ray2.68E/F18-273[»]
    1OSPX-ray1.95O18-273[»]
    2AF5X-ray2.50A27-273[»]
    2FKGX-ray2.40A-[»]
    2FKJX-ray3.10A/B/C58-273[»]
    2G8CX-ray1.15O27-273[»]
    2HKDX-ray1.60A-[»]
    2I5VX-ray1.10O27-273[»]
    2I5ZX-ray1.20O27-273[»]
    2OL6X-ray1.60O27-273[»]
    2OL7X-ray1.35A/B27-273[»]
    2OL8X-ray1.90O27-273[»]
    2OY1X-ray1.86O27-273[»]
    2OY5X-ray1.80O27-273[»]
    2OY7X-ray1.55A27-273[»]
    2OY8X-ray2.00A27-273[»]
    2OYBX-ray1.30O27-273[»]
    2PI3X-ray1.40O27-273[»]
    3AUMX-ray1.60O27-273[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CL66.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OMAiALIACKQ.
    OrthoDBiEOG6BS8PS.

    Family and domain databases

    Gene3Di3.90.930.1. 1 hit.
    InterProiIPR001809. OM_lipoprot_Borrelia.
    IPR023322. OM_lipoprot_dom.
    [Graphical view]
    PfamiPF00820. Lipoprotein_1. 1 hit.
    [Graphical view]
    PRINTSiPR00968. OUTRSURFACE.
    ProDomiPD001127. OM_lipoprot_Borrelia. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF51087. SSF51087. 1 hit.
    PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CL66-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKYLLGIGL ILALIACKQN VSSLDEKNSV SVDLPGEMKV LVSKEKNKDG    50
    KYDLIATVDK LELKGTSDKN NGSGVLEGVK ADKSKVKLTI SDDLGQTTLE 100
    VFKEDGKTLV SKKVTSKDKS STEEKFNEKG EVSEKIITRA DGTRLEYTGI 150
    KSDGSGKAKE VLKGYVLEGT LTAEKTTLVV KEGTVTLSKN ISKSGEVSVE 200
    LNDTDSSAAT KKTAAWNSGT STLTITVNSK KTKDLVFTKE NTITVQQYDS 250
    NGTKLEGSAV EITKLDEIKN ALK 273
    Length:273
    Mass (Da):29,367
    Last modified:April 5, 2011 - v1
    Checksum:iB53FC01D92F6D431
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti35 – 351P → S in strain: CA7.
    Natural varianti39 – 391K → N in strain: PBre and 21343WI.
    Natural varianti59 – 591D → H in strain: 42373NY3.
    Natural varianti90 – 901I → V in strain: CA8.
    Natural varianti114 – 1141V → A in strain: PBre.
    Natural varianti127 – 1271N → S in strain: CA8.
    Natural varianti132 – 1332VS → LP in strain: CA8.
    Natural varianti144 – 1441R → K in strain: 21343WI.
    Natural varianti149 – 1491G → E in strain: PBre and 42373NY3.
    Natural varianti164 – 1641G → S in strain: PBre.
    Natural varianti196 – 1961E → A in strain: CA8 and 21343WI.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14407 Genomic DNA. Translation: CAA32579.1.
    X85739 Genomic DNA. Translation: CAA59742.1.
    X80182 Genomic DNA. Translation: CAA56467.1.
    X69606 Genomic DNA. Translation: CAA49314.1.
    AE000790 Genomic DNA. Translation: AAC66260.1.
    L23136 Genomic DNA. Translation: AAA22951.1.
    L23137 Genomic DNA. Translation: AAA22953.1.
    L23138 Genomic DNA. Translation: AAA20947.1.
    L23139 Genomic DNA. Translation: AAA20949.1.
    L23140 Genomic DNA. Translation: AAA20951.1.
    L23141 Genomic DNA. Translation: AAA20953.1.
    L23142 Genomic DNA. Translation: AAA20955.1.
    L23143 Genomic DNA. Translation: AAA20957.1.
    L23144 Genomic DNA. Translation: AAA20959.1.
    PIRiF49209.
    G70208.
    I40265.
    S71529.
    RefSeqiNP_045688.1. NC_001857.2.
    WP_010890378.1. NC_001857.2.

    Genome annotation databases

    EnsemblBacteriaiAAC66260; AAC66260; BB_A15.
    GeneIDi1194357.
    KEGGibbu:BB_A15.
    PATRICi20558954. VBIBorBur75917_1532.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14407 Genomic DNA. Translation: CAA32579.1 .
    X85739 Genomic DNA. Translation: CAA59742.1 .
    X80182 Genomic DNA. Translation: CAA56467.1 .
    X69606 Genomic DNA. Translation: CAA49314.1 .
    AE000790 Genomic DNA. Translation: AAC66260.1 .
    L23136 Genomic DNA. Translation: AAA22951.1 .
    L23137 Genomic DNA. Translation: AAA22953.1 .
    L23138 Genomic DNA. Translation: AAA20947.1 .
    L23139 Genomic DNA. Translation: AAA20949.1 .
    L23140 Genomic DNA. Translation: AAA20951.1 .
    L23141 Genomic DNA. Translation: AAA20953.1 .
    L23142 Genomic DNA. Translation: AAA20955.1 .
    L23143 Genomic DNA. Translation: AAA20957.1 .
    L23144 Genomic DNA. Translation: AAA20959.1 .
    PIRi F49209.
    G70208.
    I40265.
    S71529.
    RefSeqi NP_045688.1. NC_001857.2.
    WP_010890378.1. NC_001857.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FJ1 X-ray 2.68 E/F 18-273 [» ]
    1OSP X-ray 1.95 O 18-273 [» ]
    2AF5 X-ray 2.50 A 27-273 [» ]
    2FKG X-ray 2.40 A - [» ]
    2FKJ X-ray 3.10 A/B/C 58-273 [» ]
    2G8C X-ray 1.15 O 27-273 [» ]
    2HKD X-ray 1.60 A - [» ]
    2I5V X-ray 1.10 O 27-273 [» ]
    2I5Z X-ray 1.20 O 27-273 [» ]
    2OL6 X-ray 1.60 O 27-273 [» ]
    2OL7 X-ray 1.35 A/B 27-273 [» ]
    2OL8 X-ray 1.90 O 27-273 [» ]
    2OY1 X-ray 1.86 O 27-273 [» ]
    2OY5 X-ray 1.80 O 27-273 [» ]
    2OY7 X-ray 1.55 A 27-273 [» ]
    2OY8 X-ray 2.00 A 27-273 [» ]
    2OYB X-ray 1.30 O 27-273 [» ]
    2PI3 X-ray 1.40 O 27-273 [» ]
    3AUM X-ray 1.60 O 27-273 [» ]
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    DrugBanki DB00045. OspA lipoprotein.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC66260 ; AAC66260 ; BB_A15 .
    GeneIDi 1194357.
    KEGGi bbu:BB_A15.
    PATRICi 20558954. VBIBorBur75917_1532.

    Phylogenomic databases

    OMAi ALIACKQ.
    OrthoDBi EOG6BS8PS.

    Enzyme and pathway databases

    BioCyci BBUR224326:G9SO-876-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0CL66.

    Family and domain databases

    Gene3Di 3.90.930.1. 1 hit.
    InterProi IPR001809. OM_lipoprot_Borrelia.
    IPR023322. OM_lipoprot_dom.
    [Graphical view ]
    Pfami PF00820. Lipoprotein_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00968. OUTRSURFACE.
    ProDomi PD001127. OM_lipoprot_Borrelia. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF51087. SSF51087. 1 hit.
    PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi."
      Bergstroem S., Bundoc V., Barbour A.G.
      Mol. Microbiol. 3:479-486(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
    2. "Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species."
      Will G., Jauris-Heipke S., Schwab E., Busch U., Roessler D., Soutschek E., Wilske B., Preac-Mursic V.
      Med. Microbiol. Immunol. 184:73-80(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KA and PBre.
    3. "Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development."
      Dykhuizen D.E., Polin D.S., Dunn J.J., Wilske B., Preac-Mursic V., Dattwyler R.J., Luft B.J.
      Proc. Natl. Acad. Sci. U.S.A. 90:10163-10167(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KA.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
    5. "Sequence variation in the outer-surface-protein genes of Borrelia burgdorferi."
      Caporale D.A., Kocher T.D.
      Mol. Biol. Evol. 11:51-64(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-273.
      Strain: 19535NY2, 21343WI, 27985CT2, 41552MA, 42373NY3, CA3, CA7, CA8 and HB19CT1.
    6. "Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab."
      Li H., Dunn J.J., Luft B.J., Lawson C.L.
      Proc. Natl. Acad. Sci. U.S.A. 94:3584-3589(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
    7. "Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA."
      Ding W., Huang X., Yang X., Dunn J.J., Luft B.J., Koide S., Lawson C.L.
      J. Mol. Biol. 302:1153-1164(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.68 ANGSTROMS).
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

    Entry informationi

    Entry nameiOSPA_BORBU
    AccessioniPrimary (citable) accession number: P0CL66
    Secondary accession number(s): P0C926
    , P14013, Q44882, Q44964, Q44967, Q44969, Q44971, Q57123, Q57272
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Plasmid, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3