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P0CL66 (OSPA_BORBU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer surface protein A
Gene names
Name:ospA
Ordered Locus Names:BB_A15
Encoded onPlasmid lp54
OrganismBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) [Reference proteome] [HAMAP]
Taxonomic identifier224326 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cell outer membrane; Lipid-anchor.

Ontologies

Keywords
   Cellular componentCell outer membrane
Membrane
   DomainSignal
   PTMLipoprotein
Palmitate
   Technical term3D-structure
Complete proteome
Plasmid
Reference proteome
Gene Ontology (GO)
   Cellular_componentcell outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616
Chain17 – 273257Outer surface protein A
PRO_0000018074

Amino acid modifications

Lipidation171N-palmitoyl cysteine Probable
Lipidation171S-diacylglycerol cysteine Probable

Natural variations

Natural variant351P → S in strain: CA7.
Natural variant391K → N in strain: PBre and 21343WI.
Natural variant591D → H in strain: 42373NY3.
Natural variant901I → V in strain: CA8.
Natural variant1141V → A in strain: PBre.
Natural variant1271N → S in strain: CA8.
Natural variant132 – 1332VS → LP in strain: CA8.
Natural variant1441R → K in strain: 21343WI.
Natural variant1491G → E in strain: PBre and 42373NY3.
Natural variant1641G → S in strain: PBre.
Natural variant1961E → A in strain: CA8 and 21343WI.

Secondary structure

......................................................... 273
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0CL66 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: B53FC01D92F6D431

FASTA27329,367
        10         20         30         40         50         60 
MKKYLLGIGL ILALIACKQN VSSLDEKNSV SVDLPGEMKV LVSKEKNKDG KYDLIATVDK 

        70         80         90        100        110        120 
LELKGTSDKN NGSGVLEGVK ADKSKVKLTI SDDLGQTTLE VFKEDGKTLV SKKVTSKDKS 

       130        140        150        160        170        180 
STEEKFNEKG EVSEKIITRA DGTRLEYTGI KSDGSGKAKE VLKGYVLEGT LTAEKTTLVV 

       190        200        210        220        230        240 
KEGTVTLSKN ISKSGEVSVE LNDTDSSAAT KKTAAWNSGT STLTITVNSK KTKDLVFTKE 

       250        260        270 
NTITVQQYDS NGTKLEGSAV EITKLDEIKN ALK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi."
Bergstroem S., Bundoc V., Barbour A.G.
Mol. Microbiol. 3:479-486(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]"Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species."
Will G., Jauris-Heipke S., Schwab E., Busch U., Roessler D., Soutschek E., Wilske B., Preac-Mursic V.
Med. Microbiol. Immunol. 184:73-80(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KA and PBre.
[3]"Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development."
Dykhuizen D.E., Polin D.S., Dunn J.J., Wilske B., Preac-Mursic V., Dattwyler R.J., Luft B.J.
Proc. Natl. Acad. Sci. U.S.A. 90:10163-10167(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KA.
[4]"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi."
Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. expand/collapse author list , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C.
Nature 390:580-586(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[5]"Sequence variation in the outer-surface-protein genes of Borrelia burgdorferi."
Caporale D.A., Kocher T.D.
Mol. Biol. Evol. 11:51-64(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-273.
Strain: 19535NY2, 21343WI, 27985CT2, 41552MA, 42373NY3, CA3, CA7, CA8 and HB19CT1.
[6]"Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab."
Li H., Dunn J.J., Luft B.J., Lawson C.L.
Proc. Natl. Acad. Sci. U.S.A. 94:3584-3589(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
[7]"Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA."
Ding W., Huang X., Yang X., Dunn J.J., Luft B.J., Koide S., Lawson C.L.
J. Mol. Biol. 302:1153-1164(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.68 ANGSTROMS).
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14407 Genomic DNA. Translation: CAA32579.1.
X85739 Genomic DNA. Translation: CAA59742.1.
X80182 Genomic DNA. Translation: CAA56467.1.
X69606 Genomic DNA. Translation: CAA49314.1.
AE000790 Genomic DNA. Translation: AAC66260.1.
L23136 Genomic DNA. Translation: AAA22951.1.
L23137 Genomic DNA. Translation: AAA22953.1.
L23138 Genomic DNA. Translation: AAA20947.1.
L23139 Genomic DNA. Translation: AAA20949.1.
L23140 Genomic DNA. Translation: AAA20951.1.
L23141 Genomic DNA. Translation: AAA20953.1.
L23142 Genomic DNA. Translation: AAA20955.1.
L23143 Genomic DNA. Translation: AAA20957.1.
L23144 Genomic DNA. Translation: AAA20959.1.
PIRF49209.
G70208.
I40265.
S71529.
RefSeqNP_045688.1. NC_001857.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FJ1X-ray2.68E/F18-273[»]
1OSPX-ray1.95O18-273[»]
2AF5X-ray2.50A27-273[»]
2FKGX-ray2.40A-[»]
2FKJX-ray3.10A/B/C58-273[»]
2G8CX-ray1.15O27-273[»]
2HKDX-ray1.60A-[»]
2I5VX-ray1.10O27-273[»]
2I5ZX-ray1.20O27-273[»]
2OL6X-ray1.60O27-273[»]
2OL7X-ray1.35A/B27-273[»]
2OL8X-ray1.90O27-273[»]
2OY1X-ray1.86O27-273[»]
2OY5X-ray1.80O27-273[»]
2OY7X-ray1.55A27-273[»]
2OY8X-ray2.00A27-273[»]
2OYBX-ray1.30O27-273[»]
2PI3X-ray1.40O27-273[»]
3AUMX-ray1.60O27-273[»]
ModBaseSearch...
MobiDBSearch...

Chemistry

DrugBankDB00045. OspA lipoprotein.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC66260; AAC66260; BB_A15.
GeneID1194357.
KEGGbbu:BB_A15.
PATRIC20558954. VBIBorBur75917_1532.

Phylogenomic databases

OMAALIACKQ.
OrthoDBEOG6BS8PS.

Enzyme and pathway databases

BioCycBBUR224326:G9SO-876-MONOMER.

Family and domain databases

Gene3D3.90.930.1. 1 hit.
InterProIPR001809. OM_lipoprot_Borrelia.
IPR023322. OM_lipoprot_dom.
[Graphical view]
PfamPF00820. Lipoprotein_1. 1 hit.
[Graphical view]
PRINTSPR00968. OUTRSURFACE.
ProDomPD001127. OM_lipoprot_Borrelia. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF51087. SSF51087. 1 hit.
PROSITEPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0CL66.

Entry information

Entry nameOSPA_BORBU
AccessionPrimary (citable) accession number: P0CL66
Secondary accession number(s): P0C926 expand/collapse secondary AC list , P14013, Q44882, Q44964, Q44967, Q44969, Q44971, Q57123, Q57272
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 5, 2011
Last modified: December 11, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references