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Protein

Peptidoglycan D,D-transpeptidase MrdA

Gene

mrdA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes cross-linking of the peptidoglycan cell wall.UniRule annotation

Catalytic activityi

Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei326Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase MrdAUniRule annotation (EC:3.4.16.4UniRule annotation)
Alternative name(s):
Penicillin-binding protein 2UniRule annotation
Short name:
PBP-2UniRule annotation
Gene namesi
Name:mrdAUniRule annotation
Synonyms:pbpA
Ordered Locus Names:STM1910
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei17 – 37HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954471 – 623Peptidoglycan D,D-transpeptidase MrdAAdd BLAST623

Proteomic databases

PaxDbiP0CL14.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1910.

Structurei

3D structure databases

ProteinModelPortaliP0CL14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transpeptidase family. MrdA subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
HOGENOMiHOG000266120.
KOiK05515.
OMAiTLACKTG.
PhylomeDBiP0CL14.

Family and domain databases

HAMAPiMF_02081. MrdA_transpept. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
PfamiView protein in Pfam
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CL14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFKDFDAEE KLFLRRVIVA FGVVVVCFGI LIFNLYNLQI RQHHYYTTRS
60 70 80 90 100
NENDIKMLPV APTRGIIYDR NGIPLVRNVT WYDIAVTPYK IADMDALLKQ
110 120 130 140 150
LTPIVDLSPD DISDFRRALK SSSRYRPVVL KNALTDVEIA RFAVNQFHFN
160 170 180 190 200
GVTINSYQDR QYPYGAELAH VLGYVSKIND NDLKALDKKG LAENYAADHN
210 220 230 240 250
IGKQGIERYY ENDLHGKTGY QEVEVDNHGR IVRLLKDVPP IAGKNIHLTL
260 270 280 290 300
DLHLQEYIES LLAGQRAAVL VEDPHDGSVL AMVSMPSYDP NPFVKGISYQ
310 320 330 340 350
DYGKLLHDKN LPLINRVTQG LYPPASTVKP YMAMSALLCG IITPQTTFFG
360 370 380 390 400
APTWTLPGTQ RHYRDWKKTG HGMLDVTKAI EESADTFFYQ VAYMMGIDRI
410 420 430 440 450
DTMLSQFGYG KPTGIDLNEE YDGLLPSRAW KQRVHKKAWY QGDTISVGIG
460 470 480 490 500
QGYWIATPIQ MVKAMVALIN NGKVIAPHLL LNEESGKTVV PYRPSGTPAQ
510 520 530 540 550
IADPASPYWG LVRQAMYGMA NAPNGTGYKF FHTAPYGIAA KSGTSQVFSL
560 570 580 590 600
KENQTYNAKM IPIRLRDHVF YTAFAPYKNP KVAIALILEN GGSDGVTAAP
610 620
IMRKILDHLF DPQADTTQPD QAP
Length:623
Mass (Da):69,833
Last modified:March 8, 2011 - v1
Checksum:iF59E950C293A5E71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20826.1.
RefSeqiNP_460867.1. NC_003197.2.
WP_000142877.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL20826; AAL20826; STM1910.
GeneIDi1253431.
KEGGistm:STM1910.
PATRICifig|99287.12.peg.2026.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMRDA_SALTY
AccessioniPrimary (citable) accession number: P0CL14
Secondary accession number(s): P74872
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 8, 2011
Last modified: June 7, 2017
This is version 40 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families