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P0CL06 (OTSA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

EC=2.4.1.15
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:otsA
Ordered Locus Names:STM1928
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 473472Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000122493

Regions

Region21 – 222UDP-glucose binding By similarity
Region365 – 3695UDP-glucose binding By similarity

Sites

Binding site101Glucose-6-phosphate By similarity
Binding site761Glucose-6-phosphate By similarity
Binding site1301Glucose-6-phosphate By similarity
Binding site2621UDP-glucose By similarity
Binding site2671UDP-glucose By similarity
Binding site3001Glucose-6-phosphate By similarity
Binding site3391UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
Site851Involved in alpha anomer selectivity By similarity
Site1551Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
P0CL06 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: E85B3F0AE58E144A

FASTA47353,581
        10         20         30         40         50         60 
MSRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDE PLKKVTKGNI 

        70         80         90        100        110        120 
TWASFNLSEQ DYEDYYCQFS NAVLWPAFHY RLDLVQFQRP AWEGYMRVNA LLADKLLPLI 

       130        140        150        160        170        180 
KENDIIWVHD YHLLPFASEL RKRGVNNRIG FFLHIPFPTP EIFNALPPHD ELLEQLCDFD 

       190        200        210        220        230        240 
LLGFQTENDR LAFLDSLSSQ TRVTTRSGKQ HIAWGKDFQT EVYPIGIEPD EIALQAAGPL 

       250        260        270        280        290        300 
PPKLAQLKAE LKNVKNIFSV ERLDYSKGLP ERFLAYEALL ENYPQHRGKI RYTQIAPTSR 

       310        320        330        340        350        360 
GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKIFRY SDVGLVTPLR 

       370        380        390        400        410        420 
DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV NPYDRDDVAA ALNRALTMPL 

       430        440        450        460        470 
AERISRHAEM LDVIVKNDIN RWQERFIHDL KEVTPRSPER QQQNNVATFP KLA 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006468 Genomic DNA. Translation: AAL20844.1.
RefSeqNP_460885.1. NC_003197.1.

3D structure databases

ProteinModelPortalP0CL06.
SMRP0CL06. Positions 2-456.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0CL06.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20844; AAL20844; STM1928.
GeneID1253449.
KEGGstm:STM1928.
PATRIC32382413. VBISalEnt20916_2045.

Phylogenomic databases

HOGENOMHOG000191478.
KOK00697.
OMAIEFMPIH.
OrthoDBEOG6CCH1M.

Enzyme and pathway databases

BioCycSENT99287:GCTI-1939-MONOMER.
UniPathwayUPA00299.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameOTSA_SALTY
AccessionPrimary (citable) accession number: P0CL06
Secondary accession number(s): P0A1Q0, Q9L893
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 8, 2011
Last modified: November 13, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways