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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10D-glucose 6-phosphateBy similarity1
Binding sitei76D-glucose 6-phosphateBy similarity1
Sitei85Involved in alpha anomer selectivityBy similarity1
Binding sitei130D-glucose 6-phosphateBy similarity1
Sitei155Involved in alpha anomer selectivityBy similarity1
Binding sitei262UDP-glucoseBy similarity1
Binding sitei267UDP-glucoseBy similarity1
Binding sitei300D-glucose 6-phosphateBy similarity1
Binding sitei339UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
UDP-glucose-glucosephosphate glucosyltransferaseBy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:STM1928
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001224932 – 473Trehalose-6-phosphate synthaseAdd BLAST472

Proteomic databases

PaxDbiP0CL06.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1928.

Structurei

3D structure databases

ProteinModelPortaliP0CL06.
SMRiP0CL06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 22UDP-glucose bindingBy similarity2
Regioni365 – 369UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
PhylomeDBiP0CL06.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CL06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDE
60 70 80 90 100
PLKKVTKGNI TWASFNLSEQ DYEDYYCQFS NAVLWPAFHY RLDLVQFQRP
110 120 130 140 150
AWEGYMRVNA LLADKLLPLI KENDIIWVHD YHLLPFASEL RKRGVNNRIG
160 170 180 190 200
FFLHIPFPTP EIFNALPPHD ELLEQLCDFD LLGFQTENDR LAFLDSLSSQ
210 220 230 240 250
TRVTTRSGKQ HIAWGKDFQT EVYPIGIEPD EIALQAAGPL PPKLAQLKAE
260 270 280 290 300
LKNVKNIFSV ERLDYSKGLP ERFLAYEALL ENYPQHRGKI RYTQIAPTSR
310 320 330 340 350
GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKIFRY
360 370 380 390 400
SDVGLVTPLR DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV
410 420 430 440 450
NPYDRDDVAA ALNRALTMPL AERISRHAEM LDVIVKNDIN RWQERFIHDL
460 470
KEVTPRSPER QQQNNVATFP KLA
Length:473
Mass (Da):53,581
Last modified:March 8, 2011 - v1
Checksum:iE85B3F0AE58E144A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20844.1.
RefSeqiNP_460885.1. NC_003197.2.
WP_000089042.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL20844; AAL20844; STM1928.
GeneIDi1253449.
KEGGistm:STM1928.
PATRICifig|99287.12.peg.2045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20844.1.
RefSeqiNP_460885.1. NC_003197.2.
WP_000089042.1. NC_003197.2.

3D structure databases

ProteinModelPortaliP0CL06.
SMRiP0CL06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1928.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Proteomic databases

PaxDbiP0CL06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20844; AAL20844; STM1928.
GeneIDi1253449.
KEGGistm:STM1928.
PATRICifig|99287.12.peg.2045.

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
PhylomeDBiP0CL06.

Enzyme and pathway databases

UniPathwayiUPA00299.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOTSA_SALTY
AccessioniPrimary (citable) accession number: P0CL06
Secondary accession number(s): P0A1Q0, Q9L893
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 8, 2011
Last modified: June 7, 2017
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.