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P0CL05

- END8_SALTY

UniProt

P0CL05 - END8_SALTY

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Protein

Endonuclease 8

Gene
nei, STM0728
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.UniRule annotation

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.UniRule annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA By similarity
Active sitei3 – 31Proton donor By similarity
Active sitei53 – 531Proton donor; for beta-elimination activity By similarity
Binding sitei70 – 701DNA By similarity
Binding sitei125 – 1251DNA By similarity
Binding sitei169 – 1691DNA By similarity
Active sitei253 – 2531Proton donor; for delta-elimination activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSENT99287:GCTI-732-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8
Alternative name(s):
DNA glycosylase/AP lyase Nei (EC:3.2.2.-, EC:4.2.99.18)
DNA-(apurinic or apyrimidinic site) lyase Nei
Endonuclease VIII
Gene namesi
Name:nei
Ordered Locus Names:STM0728
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 263262Endonuclease 8UniRule annotationPRO_0000170898Add
BLAST

Proteomic databases

PRIDEiP0CL05.

Structurei

3D structure databases

ProteinModelPortaliP0CL05.
SMRiP0CL05. Positions 2-262.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000020882.
KOiK05522.
OMAiGPDVLDM.
OrthoDBiEOG6QP131.
PhylomeDBiP0CL05.

Family and domain databases

HAMAPiMF_01253. Endonuclease_8.
InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CL05-1 [UniParc]FASTAAdd to Basket

« Hide

MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF AQLKPYESQL TGQLVTRIET    50
RGKALLTHFS NGLTLYSHNQ LYGVWRVIDT GEIPQTTRIL RVRLQTADKT 100
ILLYSASDIE MLTAEQLTTH PFLQRVGPDV LDARLTPEEV KARLLSPRFR 150
NRQFSGLLLD QSFLAGLGNY LRVEILWQVG LTGQHKAKDL NEAQLNALSH 200
ALLDIPRLSY TTRGQADENK HHGALFRFKL FHRDGEACER CGGIIEKTTL 250
SSRPFYWCPH CQK 263
Length:263
Mass (Da):29,863
Last modified:March 8, 2011 - v1
Checksum:i0BA96E876F2F19FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL19672.1.
RefSeqiNP_459713.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19672; AAL19672; STM0728.
GeneIDi1252248.
KEGGistm:STM0728.
PATRICi32379815. VBISalEnt20916_0760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL19672.1 .
RefSeqi NP_459713.1. NC_003197.1.

3D structure databases

ProteinModelPortali P0CL05.
SMRi P0CL05. Positions 2-262.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0CL05.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL19672 ; AAL19672 ; STM0728 .
GeneIDi 1252248.
KEGGi stm:STM0728.
PATRICi 32379815. VBISalEnt20916_0760.

Phylogenomic databases

HOGENOMi HOG000020882.
KOi K05522.
OMAi GPDVLDM.
OrthoDBi EOG6QP131.
PhylomeDBi P0CL05.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-732-MONOMER.

Family and domain databases

HAMAPi MF_01253. Endonuclease_8.
InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiEND8_SALTY
AccessioniPrimary (citable) accession number: P0CL05
Secondary accession number(s): Q8ZQU6, Q9F612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 8, 2011
Last modified: July 9, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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