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P0CL03

- AMPD_SALTY

UniProt

P0CL03 - AMPD_SALTY

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Protein
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Gene
ampD, STM0146
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zinc; required for amidase activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc; catalytic By similarity
Active sitei116 – 1161Proton acceptor By similarity
Metal bindingi154 – 1541Zinc; catalytic By similarity
Sitei162 – 1621Transition state stabilizer By similarity
Metal bindingi164 – 1641Zinc; catalytic By similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSENT99287:GCTI-145-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
Alternative name(s):
N-acetylmuramoyl-L-alanine amidase
Gene namesi
Name:ampD
Ordered Locus Names:STM0146
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1871871,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
PRO_0000164415Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP0CL03.
SMRiP0CL03. Positions 1-187.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000255963.
KOiK03806.
OMAiGRPRCND.
OrthoDBiEOG60GRR5.
PhylomeDBiP0CL03.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CL03-1 [UniParc]FASTAAdd to Basket

« Hide

MLPDKGWLVE ARRVPSPHYD CRPDDEKPSL LVVHNISLPP GEFGGPWIDA    50
LFTGTIDPDA HPFFAEIAHL RVSAHCLIRR DGEIVQYVPF DKRAWHAGVS 100
NYQGRERCND FSIGIELEGT DTLAYTDAQY QQLAAVTRTL IASYPAIADN 150
MTGHCNITPD RKTDPGPAFD WPRFRALVAL SSHKEMT 187
Length:187
Mass (Da):20,943
Last modified:March 8, 2011 - v1
Checksum:i9E7EBAC553BA8EA4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL19110.1.
L07292 Genomic DNA. Translation: AAA03224.1.
RefSeqiNP_459151.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19110; AAL19110; STM0146.
GeneIDi1251664.
KEGGistm:STM0146.
PATRICi32378571. VBISalEnt20916_0155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL19110.1 .
L07292 Genomic DNA. Translation: AAA03224.1 .
RefSeqi NP_459151.1. NC_003197.1.

3D structure databases

ProteinModelPortali P0CL03.
SMRi P0CL03. Positions 1-187.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL19110 ; AAL19110 ; STM0146 .
GeneIDi 1251664.
KEGGi stm:STM0146.
PATRICi 32378571. VBISalEnt20916_0155.

Phylogenomic databases

HOGENOMi HOG000255963.
KOi K03806.
OMAi GRPRCND.
OrthoDBi EOG60GRR5.
PhylomeDBi P0CL03.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-145-MONOMER.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "The Salmonella typhimurium nadC gene: sequence determination by use of Mud-P22 and purification of quinolinate phosphoribosyltransferase."
    Hughes K.T., Dessen A., Gray J.P., Grubmeyer C.
    J. Bacteriol. 175:479-486(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
    Strain: LT2.

Entry informationi

Entry nameiAMPD_SALTY
AccessioniPrimary (citable) accession number: P0CL03
Secondary accession number(s): P30013, Q9L4I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 8, 2011
Last modified: July 9, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi