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P0CL03

- AMPD_SALTY

UniProt

P0CL03 - AMPD_SALTY

Protein

1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD

Gene

ampD

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (08 Mar 2011)
      Previous versions | rss
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    Functioni

    Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc; required for amidase activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Zinc; catalyticBy similarity
    Active sitei116 – 1161Proton acceptorBy similarity
    Metal bindingi154 – 1541Zinc; catalyticBy similarity
    Sitei162 – 1621Transition state stabilizerBy similarity
    Metal bindingi164 – 1641Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-145-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
    Alternative name(s):
    N-acetylmuramoyl-L-alanine amidase
    Gene namesi
    Name:ampD
    Ordered Locus Names:STM0146
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1871871,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpDPRO_0000164415Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0CL03.
    SMRiP0CL03. Positions 1-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000255963.
    KOiK03806.
    OMAiGRPRCND.
    OrthoDBiEOG60GRR5.
    PhylomeDBiP0CL03.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0CL03-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPDKGWLVE ARRVPSPHYD CRPDDEKPSL LVVHNISLPP GEFGGPWIDA    50
    LFTGTIDPDA HPFFAEIAHL RVSAHCLIRR DGEIVQYVPF DKRAWHAGVS 100
    NYQGRERCND FSIGIELEGT DTLAYTDAQY QQLAAVTRTL IASYPAIADN 150
    MTGHCNITPD RKTDPGPAFD WPRFRALVAL SSHKEMT 187
    Length:187
    Mass (Da):20,943
    Last modified:March 8, 2011 - v1
    Checksum:i9E7EBAC553BA8EA4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19110.1.
    L07292 Genomic DNA. Translation: AAA03224.1.
    RefSeqiNP_459151.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19110; AAL19110; STM0146.
    GeneIDi1251664.
    KEGGistm:STM0146.
    PATRICi32378571. VBISalEnt20916_0155.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19110.1 .
    L07292 Genomic DNA. Translation: AAA03224.1 .
    RefSeqi NP_459151.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P0CL03.
    SMRi P0CL03. Positions 1-187.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19110 ; AAL19110 ; STM0146 .
    GeneIDi 1251664.
    KEGGi stm:STM0146.
    PATRICi 32378571. VBISalEnt20916_0155.

    Phylogenomic databases

    HOGENOMi HOG000255963.
    KOi K03806.
    OMAi GRPRCND.
    OrthoDBi EOG60GRR5.
    PhylomeDBi P0CL03.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-145-MONOMER.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "The Salmonella typhimurium nadC gene: sequence determination by use of Mud-P22 and purification of quinolinate phosphoribosyltransferase."
      Hughes K.T., Dessen A., Gray J.P., Grubmeyer C.
      J. Bacteriol. 175:479-486(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
      Strain: LT2.

    Entry informationi

    Entry nameiAMPD_SALTY
    AccessioniPrimary (citable) accession number: P0CL03
    Secondary accession number(s): P30013, Q9L4I4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: March 8, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3