P0CK95 (ACFD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipoprotein AcfD homolog

Gene names

Name:	yghJ
Ordered Locus Names:	b4466, JW5925
ORF Names:	ECK2968

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	1520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	To V.cholerae AcfD (VC_0845).
Caution	This protein is probably not frameshifted under normal conditions; however as suggested for V.cholerae acfD in strain Ogawa 395 it might be translated under certain special conditions.
Sequence caution	The sequence AAA69140.1 differs from that shown. Reason: Frameshift at position 672. The sequence AAA69141.1 differs from that shown. Reason: Frameshift at position 672.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 1520	1497	Putative lipoprotein AcfD homolog		PRO_0000020619
Amino acid modifications
	Lipidation	24	1	N-palmitoyl cysteine Potential
	Lipidation	24	1	S-diacylglycerol cysteine Potential
Natural variations
	Natural variant	1358	1	N → G in strain: O15:H- / 83/39 / ETEC.
	Natural variant	1392 – 1402	11	DGTPLPEFYSE → EGELPKFFSD in strain: O15:H- / 83/39 / ETEC.
	Natural variant	1423 – 1428	6	EVSNDK → DVGDKT in strain: O15:H- / 83/39 / ETEC.
	Natural variant	1498	1	D → K in strain: O15:H- / 83/39 / ETEC.
	Natural variant	1511	1	Q → K in strain: O15:H- / 83/39 / ETEC.
	Natural variant	1519	1	A → V in strain: O15:H- / 83/39 / ETEC.

Sequences

	Sequence		Length	Mass (Da)
	P0CK95 [UniParc]. Last modified March 8, 2011. Version 1. Checksum: C835E4953471A7B3	FASTA	1,520	167,246
	10 20 30 40 50 60 MNKKFKYKKS LLAAILSATL LAGCDGGGSG SSSDTPPVDS GTGSLPEVKP DPTPNPEPTP 70 80 90 100 110 120 EPTPDPEPTP EPIPDPEPTP EPEPEPVPTK TGYLTLGGSQ RVTGATCNGE SSDGFTFKPG 130 140 150 160 170 180 EDVTCVAGNT TIATFNTQSE AARSLRAVEK VSFSLEDAQE LAGSDDKKSN AVSLVTSSNS 190 200 210 220 230 240 CPANTEQVCL TFSSVIESKR FDSLYKQIDL APEEFKKLVN EEVENNAATD KAPSTHTSPV 250 260 270 280 290 300 VPVTTPGTKP DLNASFVSAN AEQFYQYQPT EIILSEGRLV DSQGYGVAGV NYYTNSGRGV 310 320 330 340 350 360 TGENGEFSFS WGETISFGID TFELGSVRGN KSTIALTELG DEVRGANIDQ LIHRYSTTGQ 370 380 390 400 410 420 NNTRVVPDDV RKVFAEYPNV INEIINLSLS NGATLGEGEQ VVNLPNEFIE QFNTGQAKEI 430 440 450 460 470 480 DTAICAKTDG CNEARWFSLT TRNVNDGQIQ GVINKLWGVD TNYKSVSKFH VFHDSTNFYG 490 500 510 520 530 540 STGNARGQAV VNISNAAFPI LMARNDKNYW LAFGEKRAWD KNELAYITEA PSLVEPENVT 550 560 570 580 590 600 RDTATFNLPF ISLGQVGEGK LMVIGNPHYN SILRCPNGYS WNGGVNKDGQ CTLNSDPDDM 610 620 630 640 650 660 KNFMENVLRY LSDDKWKPDA KASMTVGTNL DTVYFKRHGQ VTGNSAAFDF HPDFAGISVE 670 680 690 700 710 720 HLSSYGDLDP QEMPLLILNG FEYVTQVGND PYAIPLRADT SKPKLTQQDV TDLIAYLNKG 730 740 750 760 770 780 GSVLIMENVM SNLKEESASG FVRLLDAAGL SMALNKSVVN NDPQGYPNRV RQQRATGIWV 790 800 810 820 830 840 YERYPAVDGA LPYTIDSKTG EVKWKYQVEN KPDDKPKLEV ASWLEDVDGK QETRYAFIDE 850 860 870 880 890 900 ADHKTEDSLK AAKEKIFAAF PGLKECTNPA YHYEVNCLEY RPGTGVPVTG GMYVPQYTQL 910 920 930 940 950 960 SLNADTAKAM VQAADLGTNI QRLYQHELYF RTNGRKGERL SSVDLERLYQ NMSVWLWNDT 970 980 990 1000 1010 1020 SYRYEEGKND ELGFKTFTEF LNCYANDAYA GGTKCSADLK KSLVDNNMIY GDGSSKAGMM 1030 1040 1050 1060 1070 1080 NPSYPLNYME KPLTRLMLGR SWWDLNIKVD VEKYPGAVSE EGQNVTETIS LYSNPTKWFA 1090 1100 1110 1120 1130 1140 GNMQSTGLWA PAQKEVTIKS NANVPVTVTV ALADDLTGRE KHEVALNRPP RVTKTYSLDA 1150 1160 1170 1180 1190 1200 SGTVKFKVPY GGLIYIKGNS STNESASFTF TGVVKAPFYK DGAWKNDLNS PAPLGELESD 1210 1220 1230 1240 1250 1260 AFVYTTPKKN LNASNYTGGL EQFANDLDTF ASSMNDFYGR DSEDGKHRMF TYKNLPGHKH 1270 1280 1290 1300 1310 1320 RFTNDVQISI GDAHSGYPVM NSSFSPNSTT LPTTPLNDWL IWHEVGHNAA ETPLTVPGAT 1330 1340 1350 1360 1370 1380 EVANNVLALY MQDRYLGKMN RVADDITVAP EYLEESNNQA WARGGAGDRL LMYAQLKEWA 1390 1400 1410 1420 1430 1440 EKNFDIKKWY PDGTPLPEFY SEREGMKGWN LFQLMHRKAR GDEVSNDKFG GKNYCAESNG 1450 1460 1470 1480 1490 1500 NAADTLMLCA SWVAQTDLSE FFKKWNPGAN AYQLPGASEM SFEGGVSQSA YNTLASLDLP 1510 1520 KPEQGPETIN QVTEHKMSAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of a type II protein secretory pathway required for the secretion of heat-labile enterotoxin by enterotoxigenic Escherichia coli." Tauschek M., Gorrell R.J., Strugnell R.A., Robins-Browne R.M. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1321-1520. Strain: O15:H- / 83/39 / ETEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28377 Genomic DNA. Translation: AAA69141.1. Frameshift. U28377 Genomic DNA. Translation: AAA69140.1. Frameshift. U00096 Genomic DNA. Translation: AAT48156.1. AP009048 Genomic DNA. Translation: BAE77034.1. AF426313 Genomic DNA. Translation: AAL60194.1.
RefSeq	YP_026189.1. NC_000913.2. YP_491170.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0CK95.
ModBase	Search...
Proteomic databases
PRIDE	P0CK95.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT48156; AAT48156; b4466. BAE77034; BAE77034; BAE77034.
GeneID	12933985. 2847716.
KEGG	ecj:Y75_p2902. eco:b4466.
PATRIC	32121362. VBIEscCol129921_3068.
Organism-specific databases
EcoGene	EG12994. yghJ.
Phylogenomic databases
KO	K10939.
OMA	VVGVNYY.
Enzyme and pathway databases
BioCyc	EcoCyc:G7541-MONOMER.
Gene expression databases
Genevestigator	Q46837.
Family and domain databases
InterPro	IPR025385. DUF4092. [Graphical view]
Pfam	PF13322. DUF4092. 1 hit. [Graphical view]
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACFD_ECOLI

Accession

Primary (citable) accession number: P0CK95
Secondary accession number(s): Q2M9M2 , Q46837, Q46838, Q6BF58

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program