P0CK32 (RDRP_REOVL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase lambda-3
Short name=Lambda3
EC=2.7.7.48

Alternative name(s):
Lambda3(Pol)

Gene names

Name:

L1

Organism

Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]

Taxonomic identifier

10884 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 1

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	1267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Virion Potential. Note: Found in the inner capsid (12 copies).
Sequence similarities	Belongs to the reoviridae RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Cellular component	Virion
Ligand	Nucleotide-binding
Molecular function	Capsid protein Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA biosynthetic process Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1267	1267	RNA-directed RNA polymerase lambda-3		PRO_0000222745
Regions
	Domain	555 – 792	238	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P0CK32 [UniParc]. Last modified December 14, 2011. Version 1. Checksum: 157EBFD4D664FCDB	FASTA	1,267	142,355
	10 20 30 40 50 60 MSSMILTQFR PFIESISGIT DQSNDVFEDA AKAFSMFTRS DVYKALDEIP FSDDAMLPIP 70 80 90 100 110 120 PTIYTKPSHD SYYYIDALNR VRRKTYQGPD DVYVPNCSIV ELLEPHETLT SYGRLSEAIE 130 140 150 160 170 180 NRAKDGDSQA RIATTYGRIA ESQARQIKAP LEKFVLALLV SEAGGSLYDP VLQKYDEIPD 190 200 210 220 230 240 LSHNCPLWCF REICRHISGP LPDRAPYLYL SAGVFWLMSP RMTSAIPPLL SDLVNLAILQ 250 260 270 280 290 300 QTAGLDPSLV KLGVQICLHA AASSSYAWFI LKTKSIFPQN TLHSMYESLE GGYCPNLEWL 310 320 330 340 350 360 EPRSDYKFMY MGVMPLSTKY ARSAPSNDKK ARELGEKYGL SSVVSELRKR TKTYVKHDFA 370 380 390 400 410 420 SVRYIRDAMA CTSGIFLVRT PTETVLQEYT QSPEIKVPIP QKDWTGPVGE IRILKDTTSS 430 440 450 460 470 480 IARYLYRTWY LAAARMAAQP RTWDPLFQAI MRSQYVTARG GSGAALRESL YAINVSLPDF 490 500 510 520 530 540 KGLPVKAATK IFQAAQLANL PFSHTSVAIL ADTSMGLRNQ VQRRPRSIMP LNVPQQQVSA 550 560 570 580 590 600 PHTLTADYIN YHMNLSTTSG SAVIEKVIPL GVYASSPPNQ SINIDISACD ASITWDFFLS 610 620 630 640 650 660 VIMAAIHEGV ASGSIGKPFM GVPASIVNDE SVVGVRAARP ISGMQNMIQH LSKLYKRGFS 670 680 690 700 710 720 YRVNDSFSPG NDFTHMTTTF PSGSTATSTE HTANNSTMME TFLTVWGPEH TDDPDVLRLM 730 740 750 760 770 780 KSLTIQRNYV CQGDDGLMII DGNTAGKVNS ETIQKMLELI SKYGEEFGWK YDIAYDGTAE 790 800 810 820 830 840 YLKLYFIFGC RIPNLSRHPI VGKERANSSA EEPWPAILDQ IMGIFFNGVH DGLQWQRWIR 850 860 870 880 890 900 YSWALCCAFS RQRTMIGESV GYLQYPMWSF VYWGLPLVKV FGSDPWIFSW YMPTGDLGMY 910 920 930 940 950 960 SWISLIRPLM TRWMVANGYA TDRCSPVFGN ADYRRCFNEI KLYQGYYMAQ LPRNPTKSGR 970 980 990 1000 1010 1020 AAPREVREQF TQALSDYLMQ NPELKSRVLR GRSEWEKYGA GIIHNPPSLF DVPHKWYLGA 1030 1040 1050 1060 1070 1080 QEAATATREE LAEMDETLMR ARRHSYSSFS KLLEAYLLVK WRMCEAREPS VDLRLPLCAG 1090 1100 1110 1120 1130 1140 IDPLNSDPFL KMVSVGPMLQ STRKYFAQTL FMAKTVSGLD VNAIDSALLR LRTLGADKKA 1150 1160 1170 1180 1190 1200 LTAQLLMVGL QESEADALAG KIMLQDVSTV QLARVVNLAV PDTWMSLDFD SMFKHHVKLL 1210 1220 1230 1240 1250 1260 PKDGRHLNTD IPPRMGWLRA ILRFLGAGMV MTATGVAVDI YLEDIHGGGR ALGQRFMTWM RQEGRSA

References

[1]	"The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes." Wiener J.R., Joklik W.K. Virology 169:194-203(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution." Zhang X., Ji Y., Zhang L., Harrison S.C., Marinescu D.C., Nibert M.L., Baker T.S. Structure 13:1545-1557(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M24734 Genomic RNA. Translation: AAA47234.1.

PIR

MWXR31. A30121.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CSE	electron microscopy	7.00	1	1-1267	[»]

ProteinModelPortal

P0CK32.

ModBase

Search...

Proteomic databases

PRIDE

P0CK32.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]

Pfam

PF07925. RdRP_5. 1 hit.
[Graphical view]

PROSITE

PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CK32.

Entry information

Entry name

RDRP_REOVL

Accession

Primary (citable) accession number: P0CK32
Secondary accession number(s): A4ZY20 , P17376, P17378, Q85665

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 14, 2011
Last sequence update:	December 14, 2011
Last modified:	April 3, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program