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P0CK32

- RDRP_REOVL

UniProt

P0CK32 - RDRP_REOVL

Protein

RNA-directed RNA polymerase lambda-3

Gene

L1

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 12 (01 Oct 2014)
      Sequence version 1 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase lambda-3 (EC:2.7.7.48)
    Short name:
    Lambda3
    Alternative name(s):
    Lambda3(Pol)
    Gene namesi
    Name:L1
    OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
    Taxonomic identifieri10884 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000007253: Genome

    Subcellular locationi

    Virion Curated
    Note: Found in the inner capsid (12 copies).

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12671267RNA-directed RNA polymerase lambda-3PRO_0000222745Add
    BLAST

    Proteomic databases

    PRIDEiP0CK32.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CSEelectron microscopy7.0011-1267[»]
    ProteinModelPortaliP0CK32.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CK32.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini555 – 792238RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR012915. RdRP_5.
    IPR007097. RNA-dir_pol_reovirus.
    [Graphical view]
    PfamiPF07925. RdRP_5. 1 hit.
    [Graphical view]
    PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CK32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSMILTQFR PFIESISGIT DQSNDVFEDA AKAFSMFTRS DVYKALDEIP     50
    FSDDAMLPIP PTIYTKPSHD SYYYIDALNR VRRKTYQGPD DVYVPNCSIV 100
    ELLEPHETLT SYGRLSEAIE NRAKDGDSQA RIATTYGRIA ESQARQIKAP 150
    LEKFVLALLV SEAGGSLYDP VLQKYDEIPD LSHNCPLWCF REICRHISGP 200
    LPDRAPYLYL SAGVFWLMSP RMTSAIPPLL SDLVNLAILQ QTAGLDPSLV 250
    KLGVQICLHA AASSSYAWFI LKTKSIFPQN TLHSMYESLE GGYCPNLEWL 300
    EPRSDYKFMY MGVMPLSTKY ARSAPSNDKK ARELGEKYGL SSVVSELRKR 350
    TKTYVKHDFA SVRYIRDAMA CTSGIFLVRT PTETVLQEYT QSPEIKVPIP 400
    QKDWTGPVGE IRILKDTTSS IARYLYRTWY LAAARMAAQP RTWDPLFQAI 450
    MRSQYVTARG GSGAALRESL YAINVSLPDF KGLPVKAATK IFQAAQLANL 500
    PFSHTSVAIL ADTSMGLRNQ VQRRPRSIMP LNVPQQQVSA PHTLTADYIN 550
    YHMNLSTTSG SAVIEKVIPL GVYASSPPNQ SINIDISACD ASITWDFFLS 600
    VIMAAIHEGV ASGSIGKPFM GVPASIVNDE SVVGVRAARP ISGMQNMIQH 650
    LSKLYKRGFS YRVNDSFSPG NDFTHMTTTF PSGSTATSTE HTANNSTMME 700
    TFLTVWGPEH TDDPDVLRLM KSLTIQRNYV CQGDDGLMII DGNTAGKVNS 750
    ETIQKMLELI SKYGEEFGWK YDIAYDGTAE YLKLYFIFGC RIPNLSRHPI 800
    VGKERANSSA EEPWPAILDQ IMGIFFNGVH DGLQWQRWIR YSWALCCAFS 850
    RQRTMIGESV GYLQYPMWSF VYWGLPLVKV FGSDPWIFSW YMPTGDLGMY 900
    SWISLIRPLM TRWMVANGYA TDRCSPVFGN ADYRRCFNEI KLYQGYYMAQ 950
    LPRNPTKSGR AAPREVREQF TQALSDYLMQ NPELKSRVLR GRSEWEKYGA 1000
    GIIHNPPSLF DVPHKWYLGA QEAATATREE LAEMDETLMR ARRHSYSSFS 1050
    KLLEAYLLVK WRMCEAREPS VDLRLPLCAG IDPLNSDPFL KMVSVGPMLQ 1100
    STRKYFAQTL FMAKTVSGLD VNAIDSALLR LRTLGADKKA LTAQLLMVGL 1150
    QESEADALAG KIMLQDVSTV QLARVVNLAV PDTWMSLDFD SMFKHHVKLL 1200
    PKDGRHLNTD IPPRMGWLRA ILRFLGAGMV MTATGVAVDI YLEDIHGGGR 1250
    ALGQRFMTWM RQEGRSA 1267
    Length:1,267
    Mass (Da):142,355
    Last modified:December 14, 2011 - v1
    Checksum:i157EBFD4D664FCDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24734 Genomic RNA. Translation: AAA47234.1.
    PIRiA30121. MWXR31.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24734 Genomic RNA. Translation: AAA47234.1 .
    PIRi A30121. MWXR31.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CSE electron microscopy 7.00 1 1-1267 [» ]
    ProteinModelPortali P0CK32.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0CK32.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0CK32.

    Family and domain databases

    InterProi IPR012915. RdRP_5.
    IPR007097. RNA-dir_pol_reovirus.
    [Graphical view ]
    Pfami PF07925. RdRP_5. 1 hit.
    [Graphical view ]
    PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes."
      Wiener J.R., Joklik W.K.
      Virology 169:194-203(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution."
      Zhang X., Ji Y., Zhang L., Harrison S.C., Marinescu D.C., Nibert M.L., Baker T.S.
      Structure 13:1545-1557(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.0 ANGSTROMS).

    Entry informationi

    Entry nameiRDRP_REOVL
    AccessioniPrimary (citable) accession number: P0CK32
    Secondary accession number(s): A4ZY20
    , P17376, P17378, Q85665
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 14, 2011
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 12 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3