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Protein

RNA-directed RNA polymerase lambda-3

Gene

L1

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase lambda-3 (EC:2.7.7.48)
Short name:
Lambda3
Alternative name(s):
Lambda3(Pol)
Gene namesi
Name:L1
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
Proteomesi
  • UP000006373 Componenti: Genome

Subcellular locationi

  • Virion Curated

  • Note: Found in the inner capsid (12 copies).

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002227431 – 1267RNA-directed RNA polymerase lambda-3Add BLAST1267

Structurei

Secondary structure

11267
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 15Combined sources12
Turni16 – 18Combined sources3
Helixi24 – 36Combined sources13
Helixi39 – 47Combined sources9
Helixi53 – 55Combined sources3
Helixi61 – 63Combined sources3
Helixi69 – 72Combined sources4
Beta strandi73 – 75Combined sources3
Beta strandi81 – 83Combined sources3
Turni89 – 92Combined sources4
Helixi100 – 102Combined sources3
Beta strandi103 – 105Combined sources3
Beta strandi112 – 115Combined sources4
Helixi117 – 124Combined sources8
Helixi128 – 144Combined sources17
Beta strandi147 – 149Combined sources3
Helixi151 – 162Combined sources12
Turni170 – 173Combined sources4
Helixi181 – 183Combined sources3
Helixi185 – 198Combined sources14
Beta strandi206 – 209Combined sources4
Beta strandi214 – 217Combined sources4
Helixi226 – 242Combined sources17
Helixi247 – 262Combined sources16
Helixi266 – 273Combined sources8
Helixi276 – 279Combined sources4
Helixi282 – 284Combined sources3
Beta strandi291 – 299Combined sources9
Helixi303 – 305Combined sources3
Beta strandi307 – 315Combined sources9
Helixi318 – 320Combined sources3
Helixi328 – 337Combined sources10
Helixi341 – 352Combined sources12
Beta strandi356 – 358Combined sources3
Helixi359 – 369Combined sources11
Helixi381 – 388Combined sources8
Helixi401 – 403Combined sources3
Beta strandi404 – 408Combined sources5
Beta strandi411 – 414Combined sources4
Helixi422 – 438Combined sources17
Helixi440 – 443Combined sources4
Helixi445 – 453Combined sources9
Beta strandi457 – 459Combined sources3
Helixi463 – 471Combined sources9
Turni472 – 474Combined sources3
Beta strandi487 – 489Combined sources3
Helixi490 – 497Combined sources8
Helixi502 – 505Combined sources4
Helixi506 – 510Combined sources5
Beta strandi516 – 521Combined sources6
Beta strandi523 – 528Combined sources6
Helixi533 – 552Combined sources20
Helixi563 – 565Combined sources3
Helixi568 – 575Combined sources8
Beta strandi580 – 587Combined sources8
Helixi589 – 592Combined sources4
Turni595 – 597Combined sources3
Helixi598 – 609Combined sources12
Helixi611 – 614Combined sources4
Beta strandi625 – 629Combined sources5
Beta strandi632 – 634Combined sources3
Beta strandi637 – 641Combined sources5
Helixi643 – 657Combined sources15
Beta strandi659 – 664Combined sources6
Beta strandi666 – 668Combined sources3
Beta strandi672 – 677Combined sources6
Beta strandi682 – 686Combined sources5
Helixi687 – 704Combined sources18
Helixi706 – 709Combined sources4
Helixi714 – 721Combined sources8
Turni725 – 727Combined sources3
Beta strandi728 – 732Combined sources5
Beta strandi735 – 740Combined sources6
Helixi750 – 765Combined sources16
Turni766 – 768Combined sources3
Beta strandi770 – 774Combined sources5
Beta strandi779 – 781Combined sources3
Beta strandi784 – 787Combined sources4
Beta strandi790 – 792Combined sources3
Helixi795 – 797Combined sources3
Beta strandi800 – 802Combined sources3
Beta strandi807 – 809Combined sources3
Helixi816 – 830Combined sources15
Helixi835 – 849Combined sources15
Beta strandi850 – 856Combined sources7
Beta strandi859 – 864Combined sources6
Helixi867 – 872Combined sources6
Helixi895 – 916Combined sources22
Beta strandi920 – 925Combined sources6
Turni926 – 928Combined sources3
Beta strandi929 – 931Combined sources3
Helixi933 – 939Combined sources7
Helixi942 – 949Combined sources8
Helixi966 – 978Combined sources13
Helixi982 – 998Combined sources17
Helixi1009 – 1011Combined sources3
Helixi1012 – 1022Combined sources11
Helixi1028 – 1043Combined sources16
Helixi1051 – 1056Combined sources6
Beta strandi1059 – 1066Combined sources8
Helixi1086 – 1095Combined sources10
Helixi1102 – 1106Combined sources5
Helixi1107 – 1109Combined sources3
Helixi1121 – 1133Combined sources13
Helixi1138 – 1146Combined sources9
Turni1147 – 1149Combined sources3
Helixi1152 – 1164Combined sources13
Helixi1169 – 1173Combined sources5
Helixi1174 – 1176Combined sources3
Helixi1182 – 1184Combined sources3
Helixi1189 – 1195Combined sources7
Beta strandi1196 – 1198Combined sources3
Turni1207 – 1209Combined sources3
Helixi1213 – 1217Combined sources5
Helixi1218 – 1232Combined sources15
Beta strandi1233 – 1236Combined sources4
Beta strandi1238 – 1245Combined sources8
Helixi1248 – 1262Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MUKX-ray2.50A1-1267[»]
1MWHX-ray2.50A1-1267[»]
1N1HX-ray2.80A1-1267[»]
1N35X-ray2.50A1-1267[»]
1N38X-ray2.80A1-1267[»]
1UONelectron microscopy7.60A1-1267[»]
ProteinModelPortaliP0CK31.
SMRiP0CK31.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CK31.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini555 – 792RdRp catalyticPROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamiPF07925. RdRP_5. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CK31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSMILTQFG PFIESISGIT DQSNDVFEDA AKAFSMFTRS DVYKALDEIP
60 70 80 90 100
FSDDAMLPIP PTIYTKPSHD SYYYIDALNR VRRKTYQGPD DVYVPNCSIV
110 120 130 140 150
ELLEPHETLT SYGRLSEAIE NRAKDGDSQA RIATTYGRIA ESQARQIKAP
160 170 180 190 200
LEKFVLALLV AEAGGSLYDP VLQKYDEIPD LSHNCPLWCF REICRHISGP
210 220 230 240 250
LPDRAPYLYL SAGVFWLMSP RMTSAIPPLL SDLVNLAILQ QTAGLDPSLV
260 270 280 290 300
KLGVQICLHA AASSSYSWFI LKTKSIFPQN TLHSMYESLE GGYCPNLEWL
310 320 330 340 350
EPRSDYKFMY MGVMPLSAKY ARSAPSNDKK ARELGEKYGL SSVVGELRKR
360 370 380 390 400
TKTYVKHDFA SVRYIRDAMA CTSGIFLVRT PTETVLQEYT QSPEIKVPIP
410 420 430 440 450
QKDWTGPIGE IRILKDTTSS IARYLYRTWY LAAARMAAQP RTWDPLFQAI
460 470 480 490 500
MRSQYVTARG GSGAALRESL YAINVSLPDF KGLPVKAATK IFQAAQLANL
510 520 530 540 550
PFSHTSVAIL ADTSMGLRNQ VQRRPRSIMP LNVPQQQVSA PHTLTADYIN
560 570 580 590 600
YHMNLSPTSG SAVIEKVIPL GVYASSPPNQ SINIDISACD ASITWDFFLS
610 620 630 640 650
VIMAAIHEGV ASSSIGKPFM GVPASIVNDE SVVGVRAARP ISGMQNMIQH
660 670 680 690 700
LSKLYKRGFS YRVNDSFSPG NDFTHMTTTF PSGSTATSTE HTANNSTMME
710 720 730 740 750
TFLTVWGPEH TDDPDVLRLM KSLTIQRNYV CQGDDGLMII DGTTAGKVNS
760 770 780 790 800
ETIQNDLELI SKYGEEFGWK YDIAYDGTAE YLKLYFIFGC RIPNLSRHPI
810 820 830 840 850
VGKERANSSA EEPWPAILDQ IMGVFFNGVH DGLQWQRWIR YSWALCCAFS
860 870 880 890 900
RQRTMIGESV GYLQYPMWSF VYWGLPLVKA FGSDPWIFSW YMPTGDLGMY
910 920 930 940 950
SWISLIRPLM TRWMVANGYV TDRCSTVFGN ADYRRCFNEL KLYQGYYMAQ
960 970 980 990 1000
LPRNPKKSGR AASREVREQF TQALSDYLMQ NPELKSRVLR GRSEWEKYGA
1010 1020 1030 1040 1050
GIIHNPPSLF DVPHKWYQGA QEAAIATREE LAEMDETLMR ARRHSYSSFS
1060 1070 1080 1090 1100
KLLEAYLLVK WRMCEAREPS VDLRLPLCAG IDPLNSDPFL KMVSVGPMLQ
1110 1120 1130 1140 1150
STRKYFAQTL FMAKTVSGLD VNAIDSALLR LRTLGADKKA LTAQLLMVGL
1160 1170 1180 1190 1200
QESEADALAG KIMLQDVNTV QLARVVNLAV PDTWMSLDFD SMFKHHVKLL
1210 1220 1230 1240 1250
PKDGRHLNTD IPPRMGWLRA ILRFLGAGMV MTATGVAVDI YLEDIHGGGR
1260
SLGQRFMTWM RQEGRSA
Length:1,267
Mass (Da):142,270
Last modified:December 14, 2011 - v1
Checksum:iFC9FD43CEF527148
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti267S → A in ABP48913 (PubMed:18005692).Curated1
Sequence conflicti465 – 468ALRE → DSAN in AAA47255 (PubMed:2922925).Curated4
Sequence conflicti557P → T in ABP48913 (PubMed:18005692).Curated1
Sequence conflicti755 – 756ND → KM in ABP48913 (PubMed:18005692).Curated2
Sequence conflicti926T → P in ABP48913 (PubMed:18005692).Curated1
Sequence conflicti963S → P in ABP48913 (PubMed:18005692).Curated1
Sequence conflicti1048S → N in ABP48913 (PubMed:18005692).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31058 Genomic RNA. Translation: AAA47255.1.
EF494435 Genomic RNA. Translation: ABP48913.1.
PIRiC30121. MWXR33.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31058 Genomic RNA. Translation: AAA47255.1.
EF494435 Genomic RNA. Translation: ABP48913.1.
PIRiC30121. MWXR33.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MUKX-ray2.50A1-1267[»]
1MWHX-ray2.50A1-1267[»]
1N1HX-ray2.80A1-1267[»]
1N35X-ray2.50A1-1267[»]
1N38X-ray2.80A1-1267[»]
1UONelectron microscopy7.60A1-1267[»]
ProteinModelPortaliP0CK31.
SMRiP0CK31.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP0CK31.

Family and domain databases

InterProiIPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamiPF07925. RdRP_5. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRDRP_REOVD
AccessioniPrimary (citable) accession number: P0CK31
Secondary accession number(s): A4ZY20
, P17376, P17378, Q85665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: December 14, 2011
Last modified: November 2, 2016
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.