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P0CK31 (RDRP_REOVD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase lambda-3
Short name=Lambda3
EC=2.7.7.48
Alternative name(s):
Lambda3(Pol)
Gene names
Name:L1
OrganismReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]
Taxonomic identifier10886 [NCBI]
Taxonomic lineageViruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus ›
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length1267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subcellular location

Virion Potential. Note: Found in the inner capsid (12 copies).

Sequence similarities

Belongs to the reoviridae RNA-directed RNA polymerase family.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12671267RNA-directed RNA polymerase lambda-3
PRO_0000222743

Regions

Domain555 – 792238RdRp catalytic

Experimental info

Sequence conflict2671S → A in ABP48913. Ref.2
Sequence conflict465 – 4684ALRE → DSAN in AAA47255. Ref.1
Sequence conflict5571P → T in ABP48913. Ref.2
Sequence conflict755 – 7562ND → KM in ABP48913. Ref.2
Sequence conflict9261T → P in ABP48913. Ref.2
Sequence conflict9631S → P in ABP48913. Ref.2
Sequence conflict10481S → N in ABP48913. Ref.2

Secondary structure

1 ....................................................................................................................................................................................................................... 1267
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0CK31 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: FC9FD43CEF527148

FASTA1,267142,270
        10         20         30         40         50         60 
MSSMILTQFG PFIESISGIT DQSNDVFEDA AKAFSMFTRS DVYKALDEIP FSDDAMLPIP 

        70         80         90        100        110        120 
PTIYTKPSHD SYYYIDALNR VRRKTYQGPD DVYVPNCSIV ELLEPHETLT SYGRLSEAIE 

       130        140        150        160        170        180 
NRAKDGDSQA RIATTYGRIA ESQARQIKAP LEKFVLALLV AEAGGSLYDP VLQKYDEIPD 

       190        200        210        220        230        240 
LSHNCPLWCF REICRHISGP LPDRAPYLYL SAGVFWLMSP RMTSAIPPLL SDLVNLAILQ 

       250        260        270        280        290        300 
QTAGLDPSLV KLGVQICLHA AASSSYSWFI LKTKSIFPQN TLHSMYESLE GGYCPNLEWL 

       310        320        330        340        350        360 
EPRSDYKFMY MGVMPLSAKY ARSAPSNDKK ARELGEKYGL SSVVGELRKR TKTYVKHDFA 

       370        380        390        400        410        420 
SVRYIRDAMA CTSGIFLVRT PTETVLQEYT QSPEIKVPIP QKDWTGPIGE IRILKDTTSS 

       430        440        450        460        470        480 
IARYLYRTWY LAAARMAAQP RTWDPLFQAI MRSQYVTARG GSGAALRESL YAINVSLPDF 

       490        500        510        520        530        540 
KGLPVKAATK IFQAAQLANL PFSHTSVAIL ADTSMGLRNQ VQRRPRSIMP LNVPQQQVSA 

       550        560        570        580        590        600 
PHTLTADYIN YHMNLSPTSG SAVIEKVIPL GVYASSPPNQ SINIDISACD ASITWDFFLS 

       610        620        630        640        650        660 
VIMAAIHEGV ASSSIGKPFM GVPASIVNDE SVVGVRAARP ISGMQNMIQH LSKLYKRGFS 

       670        680        690        700        710        720 
YRVNDSFSPG NDFTHMTTTF PSGSTATSTE HTANNSTMME TFLTVWGPEH TDDPDVLRLM 

       730        740        750        760        770        780 
KSLTIQRNYV CQGDDGLMII DGTTAGKVNS ETIQNDLELI SKYGEEFGWK YDIAYDGTAE 

       790        800        810        820        830        840 
YLKLYFIFGC RIPNLSRHPI VGKERANSSA EEPWPAILDQ IMGVFFNGVH DGLQWQRWIR 

       850        860        870        880        890        900 
YSWALCCAFS RQRTMIGESV GYLQYPMWSF VYWGLPLVKA FGSDPWIFSW YMPTGDLGMY 

       910        920        930        940        950        960 
SWISLIRPLM TRWMVANGYV TDRCSTVFGN ADYRRCFNEL KLYQGYYMAQ LPRNPKKSGR 

       970        980        990       1000       1010       1020 
AASREVREQF TQALSDYLMQ NPELKSRVLR GRSEWEKYGA GIIHNPPSLF DVPHKWYQGA 

      1030       1040       1050       1060       1070       1080 
QEAAIATREE LAEMDETLMR ARRHSYSSFS KLLEAYLLVK WRMCEAREPS VDLRLPLCAG 

      1090       1100       1110       1120       1130       1140 
IDPLNSDPFL KMVSVGPMLQ STRKYFAQTL FMAKTVSGLD VNAIDSALLR LRTLGADKKA 

      1150       1160       1170       1180       1190       1200 
LTAQLLMVGL QESEADALAG KIMLQDVNTV QLARVVNLAV PDTWMSLDFD SMFKHHVKLL 

      1210       1220       1230       1240       1250       1260 
PKDGRHLNTD IPPRMGWLRA ILRFLGAGMV MTATGVAVDI YLEDIHGGGR SLGQRFMTWM 


RQEGRSA

« Hide

References

[1]"The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes."
Wiener J.R., Joklik W.K.
Virology 169:194-203(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"A plasmid-based reverse genetics system for animal double-stranded RNA viruses."
Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S.
Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Infectious clone.
[3]"RNA synthesis in a cage -- structural studies of reovirus polymerase lambda3."
Tao Y., Farsetta D.L., Nibert M.L., Harrison S.C.
Cell 111:733-745(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M31058 Genomic RNA. Translation: AAA47255.1.
EF494435 Genomic RNA. Translation: ABP48913.1.
PIRMWXR33. C30121.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MUKX-ray2.50A1-1267[»]
1MWHX-ray2.50A1-1267[»]
1N1HX-ray2.80A1-1267[»]
1N35X-ray2.50A1-1267[»]
1N38X-ray2.80A1-1267[»]
1UONelectron microscopy7.60A1-1267[»]
ProteinModelPortalP0CK31.
SMRP0CK31. Positions 2-1265.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamPF07925. RdRP_5. 1 hit.
[Graphical view]
PROSITEPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0CK31.

Entry information

Entry nameRDRP_REOVD
AccessionPrimary (citable) accession number: P0CK31
Secondary accession number(s): A4ZY20 expand/collapse secondary AC list , P17376, P17378, Q85665
Entry history
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: December 14, 2011
Last modified: April 3, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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