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P0CK31

- RDRP_REOVD

UniProt

P0CK31 - RDRP_REOVD

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Protein

RNA-directed RNA polymerase lambda-3

Gene

L1

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW
  3. RNA binding Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase lambda-3 (EC:2.7.7.48)
Short name:
Lambda3
Alternative name(s):
Lambda3(Pol)
Gene namesi
Name:L1
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006373: Genome

Subcellular locationi

Virion Curated
Note: Found in the inner capsid (12 copies).

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12671267RNA-directed RNA polymerase lambda-3PRO_0000222743Add
BLAST

Structurei

Secondary structure

1
1267
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1512Combined sources
Turni16 – 183Combined sources
Helixi24 – 3613Combined sources
Helixi39 – 479Combined sources
Helixi53 – 553Combined sources
Helixi61 – 633Combined sources
Helixi69 – 724Combined sources
Beta strandi73 – 753Combined sources
Beta strandi81 – 833Combined sources
Turni89 – 924Combined sources
Helixi100 – 1023Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi112 – 1154Combined sources
Helixi117 – 1248Combined sources
Helixi128 – 14417Combined sources
Beta strandi147 – 1493Combined sources
Helixi151 – 16212Combined sources
Turni170 – 1734Combined sources
Helixi181 – 1833Combined sources
Helixi185 – 19814Combined sources
Beta strandi206 – 2094Combined sources
Beta strandi214 – 2174Combined sources
Helixi226 – 24217Combined sources
Helixi247 – 26216Combined sources
Helixi266 – 2738Combined sources
Helixi276 – 2794Combined sources
Helixi282 – 2843Combined sources
Beta strandi291 – 2999Combined sources
Helixi303 – 3053Combined sources
Beta strandi307 – 3159Combined sources
Helixi318 – 3203Combined sources
Helixi328 – 33710Combined sources
Helixi341 – 35212Combined sources
Beta strandi356 – 3583Combined sources
Helixi359 – 36911Combined sources
Helixi381 – 3888Combined sources
Helixi401 – 4033Combined sources
Beta strandi404 – 4085Combined sources
Beta strandi411 – 4144Combined sources
Helixi422 – 43817Combined sources
Helixi440 – 4434Combined sources
Helixi445 – 4539Combined sources
Beta strandi457 – 4593Combined sources
Helixi463 – 4719Combined sources
Turni472 – 4743Combined sources
Beta strandi487 – 4893Combined sources
Helixi490 – 4978Combined sources
Helixi502 – 5054Combined sources
Helixi506 – 5105Combined sources
Beta strandi516 – 5216Combined sources
Beta strandi523 – 5286Combined sources
Helixi533 – 55220Combined sources
Helixi563 – 5653Combined sources
Helixi568 – 5758Combined sources
Beta strandi580 – 5878Combined sources
Helixi589 – 5924Combined sources
Turni595 – 5973Combined sources
Helixi598 – 60912Combined sources
Helixi611 – 6144Combined sources
Beta strandi625 – 6295Combined sources
Beta strandi632 – 6343Combined sources
Beta strandi637 – 6415Combined sources
Helixi643 – 65715Combined sources
Beta strandi659 – 6646Combined sources
Beta strandi666 – 6683Combined sources
Beta strandi672 – 6776Combined sources
Beta strandi682 – 6865Combined sources
Helixi687 – 70418Combined sources
Helixi706 – 7094Combined sources
Helixi714 – 7218Combined sources
Turni725 – 7273Combined sources
Beta strandi728 – 7325Combined sources
Beta strandi735 – 7406Combined sources
Helixi750 – 76516Combined sources
Turni766 – 7683Combined sources
Beta strandi770 – 7745Combined sources
Beta strandi779 – 7813Combined sources
Beta strandi784 – 7874Combined sources
Beta strandi790 – 7923Combined sources
Helixi795 – 7973Combined sources
Beta strandi800 – 8023Combined sources
Beta strandi807 – 8093Combined sources
Helixi816 – 83015Combined sources
Helixi835 – 84915Combined sources
Beta strandi850 – 8567Combined sources
Beta strandi859 – 8646Combined sources
Helixi867 – 8726Combined sources
Helixi895 – 91622Combined sources
Beta strandi920 – 9256Combined sources
Turni926 – 9283Combined sources
Beta strandi929 – 9313Combined sources
Helixi933 – 9397Combined sources
Helixi942 – 9498Combined sources
Helixi966 – 97813Combined sources
Helixi982 – 99817Combined sources
Helixi1009 – 10113Combined sources
Helixi1012 – 102211Combined sources
Helixi1028 – 104316Combined sources
Helixi1051 – 10566Combined sources
Beta strandi1059 – 10668Combined sources
Helixi1086 – 109510Combined sources
Helixi1102 – 11065Combined sources
Helixi1107 – 11093Combined sources
Helixi1121 – 113313Combined sources
Helixi1138 – 11469Combined sources
Turni1147 – 11493Combined sources
Helixi1152 – 116413Combined sources
Helixi1169 – 11735Combined sources
Helixi1174 – 11763Combined sources
Helixi1182 – 11843Combined sources
Helixi1189 – 11957Combined sources
Beta strandi1196 – 11983Combined sources
Turni1207 – 12093Combined sources
Helixi1213 – 12175Combined sources
Helixi1218 – 123215Combined sources
Beta strandi1233 – 12364Combined sources
Beta strandi1238 – 12458Combined sources
Helixi1248 – 126215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MUKX-ray2.50A1-1267[»]
1MWHX-ray2.50A1-1267[»]
1N1HX-ray2.80A1-1267[»]
1N35X-ray2.50A1-1267[»]
1N38X-ray2.80A1-1267[»]
1UONelectron microscopy7.60A1-1267[»]
ProteinModelPortaliP0CK31.
SMRiP0CK31. Positions 2-1265.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CK31.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 792238RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamiPF07925. RdRP_5. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CK31-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSMILTQFG PFIESISGIT DQSNDVFEDA AKAFSMFTRS DVYKALDEIP
60 70 80 90 100
FSDDAMLPIP PTIYTKPSHD SYYYIDALNR VRRKTYQGPD DVYVPNCSIV
110 120 130 140 150
ELLEPHETLT SYGRLSEAIE NRAKDGDSQA RIATTYGRIA ESQARQIKAP
160 170 180 190 200
LEKFVLALLV AEAGGSLYDP VLQKYDEIPD LSHNCPLWCF REICRHISGP
210 220 230 240 250
LPDRAPYLYL SAGVFWLMSP RMTSAIPPLL SDLVNLAILQ QTAGLDPSLV
260 270 280 290 300
KLGVQICLHA AASSSYSWFI LKTKSIFPQN TLHSMYESLE GGYCPNLEWL
310 320 330 340 350
EPRSDYKFMY MGVMPLSAKY ARSAPSNDKK ARELGEKYGL SSVVGELRKR
360 370 380 390 400
TKTYVKHDFA SVRYIRDAMA CTSGIFLVRT PTETVLQEYT QSPEIKVPIP
410 420 430 440 450
QKDWTGPIGE IRILKDTTSS IARYLYRTWY LAAARMAAQP RTWDPLFQAI
460 470 480 490 500
MRSQYVTARG GSGAALRESL YAINVSLPDF KGLPVKAATK IFQAAQLANL
510 520 530 540 550
PFSHTSVAIL ADTSMGLRNQ VQRRPRSIMP LNVPQQQVSA PHTLTADYIN
560 570 580 590 600
YHMNLSPTSG SAVIEKVIPL GVYASSPPNQ SINIDISACD ASITWDFFLS
610 620 630 640 650
VIMAAIHEGV ASSSIGKPFM GVPASIVNDE SVVGVRAARP ISGMQNMIQH
660 670 680 690 700
LSKLYKRGFS YRVNDSFSPG NDFTHMTTTF PSGSTATSTE HTANNSTMME
710 720 730 740 750
TFLTVWGPEH TDDPDVLRLM KSLTIQRNYV CQGDDGLMII DGTTAGKVNS
760 770 780 790 800
ETIQNDLELI SKYGEEFGWK YDIAYDGTAE YLKLYFIFGC RIPNLSRHPI
810 820 830 840 850
VGKERANSSA EEPWPAILDQ IMGVFFNGVH DGLQWQRWIR YSWALCCAFS
860 870 880 890 900
RQRTMIGESV GYLQYPMWSF VYWGLPLVKA FGSDPWIFSW YMPTGDLGMY
910 920 930 940 950
SWISLIRPLM TRWMVANGYV TDRCSTVFGN ADYRRCFNEL KLYQGYYMAQ
960 970 980 990 1000
LPRNPKKSGR AASREVREQF TQALSDYLMQ NPELKSRVLR GRSEWEKYGA
1010 1020 1030 1040 1050
GIIHNPPSLF DVPHKWYQGA QEAAIATREE LAEMDETLMR ARRHSYSSFS
1060 1070 1080 1090 1100
KLLEAYLLVK WRMCEAREPS VDLRLPLCAG IDPLNSDPFL KMVSVGPMLQ
1110 1120 1130 1140 1150
STRKYFAQTL FMAKTVSGLD VNAIDSALLR LRTLGADKKA LTAQLLMVGL
1160 1170 1180 1190 1200
QESEADALAG KIMLQDVNTV QLARVVNLAV PDTWMSLDFD SMFKHHVKLL
1210 1220 1230 1240 1250
PKDGRHLNTD IPPRMGWLRA ILRFLGAGMV MTATGVAVDI YLEDIHGGGR
1260
SLGQRFMTWM RQEGRSA
Length:1,267
Mass (Da):142,270
Last modified:December 14, 2011 - v1
Checksum:iFC9FD43CEF527148
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti267 – 2671S → A in ABP48913. (PubMed:18005692)Curated
Sequence conflicti465 – 4684ALRE → DSAN in AAA47255. (PubMed:2922925)Curated
Sequence conflicti557 – 5571P → T in ABP48913. (PubMed:18005692)Curated
Sequence conflicti755 – 7562ND → KM in ABP48913. (PubMed:18005692)Curated
Sequence conflicti926 – 9261T → P in ABP48913. (PubMed:18005692)Curated
Sequence conflicti963 – 9631S → P in ABP48913. (PubMed:18005692)Curated
Sequence conflicti1048 – 10481S → N in ABP48913. (PubMed:18005692)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31058 Genomic RNA. Translation: AAA47255.1.
EF494435 Genomic RNA. Translation: ABP48913.1.
PIRiC30121. MWXR33.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31058 Genomic RNA. Translation: AAA47255.1 .
EF494435 Genomic RNA. Translation: ABP48913.1 .
PIRi C30121. MWXR33.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MUK X-ray 2.50 A 1-1267 [» ]
1MWH X-ray 2.50 A 1-1267 [» ]
1N1H X-ray 2.80 A 1-1267 [» ]
1N35 X-ray 2.50 A 1-1267 [» ]
1N38 X-ray 2.80 A 1-1267 [» ]
1UON electron microscopy 7.60 A 1-1267 [» ]
ProteinModelPortali P0CK31.
SMRi P0CK31. Positions 2-1265.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P0CK31.

Family and domain databases

InterProi IPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view ]
Pfami PF07925. RdRP_5. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes."
    Wiener J.R., Joklik W.K.
    Virology 169:194-203(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Infectious clone.
  3. "RNA synthesis in a cage -- structural studies of reovirus polymerase lambda3."
    Tao Y., Farsetta D.L., Nibert M.L., Harrison S.C.
    Cell 111:733-745(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiRDRP_REOVD
AccessioniPrimary (citable) accession number: P0CK31
Secondary accession number(s): A4ZY20
, P17376, P17378, Q85665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: December 14, 2011
Last modified: November 26, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3