P0CJ81 (P65_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline-rich P65 protein

Gene names

Name:	p65
Ordered Locus Names:	MPN_309
ORF Names:	MP527

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Subcellular location	Cell membrane; Peripheral membrane protein. Note: Probably with epitopes exposed at the cell surface. Ref.1 Ref.4
Domain	The penta/hexapeptides repeats form a proline-rich acidic domain. In addition, a part of this region contains a perfect direct repeat.
Post-translational modification	The N-terminus is blocked Probable.
Caution	The protein sequence for 83-95 (Ref.4) might be that for residues 148-160; the 2 sequences are identical.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Proline-rich P65 protein		PRO_0000058145
Regions
	Repeat	40 – 45	6	1
	Repeat	75 – 80	6	2
	Repeat	83 – 87	5	3
	Repeat	89 – 93	5	4
	Repeat	95 – 99	5	5
	Repeat	101 – 105	5	6
	Repeat	107 – 111	5	7
	Repeat	119 – 123	5	8
	Repeat	140 – 145	6	9
	Repeat	148 – 152	5	10
	Repeat	154 – 158	5	11
	Repeat	168 – 172	5	12
	Region	40 – 172	133	12 X 5 AA repeats of D-P-N-Q-A-Y

Sequences

	Sequence		Length	Mass (Da)
	P0CJ81 [UniParc]. Last modified February 8, 2011. Version 1. Checksum: 4805F2DAD0588232	FASTA	405	47,040
	10 20 30 40 50 60 MDINKPGWNQ SDQQATAYDP NQQQYYGDGS TYYDPDQAVD PNQAYYPDPN TYPDAAAYYG 70 80 90 100 110 120 YGQDGQAYPQ DYAQDPNQAY YADPNAYQDP NAYTDPNAYV DPNAYQDPNA YVDPNNYTDP 130 140 150 160 170 180 NAYYGYGQDG QAYPQDYAQD PNQAYYADPN AYQDPNAYTD PYYVTSTDPN AYYGQVDNVP 190 200 210 220 230 240 ALEASDLAYE VTPQEQAAEQ ELFSEPETKV IREIHEFPFE KIRSYFQTDF DSYNSRLTQL 250 260 270 280 290 300 KDKLDNAIFS MRKAIDTVKE NSANLQIMKQ NFERQLKEQQ TQRLTSNTDA EKIGAKINQL 310 320 330 340 350 360 EERMQRLSRT MESVEWTKKE PRQEQFDPRF VDPRNFNNYV NNTDTMMSMF EKVLMMNLLR 370 380 390 400 STTPVQPPVQ YFTPQPLTAS PRPVYEEPIS ASFRRRGYRG DEFYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proline-rich P65 protein of Mycoplasma pneumoniae is a component of the Triton X-100-insoluble fraction and exhibits size polymorphism in the strains M129 and FH." Proft T., Hilbert H., Layh-Schmitt G., Herrmann R. J. Bacteriol. 177:3370-3378(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION. Strain: ATCC 29342 / M129.
[2]	"Transposon mutagenesis reinforces the correlation between Mycoplasma pneumoniae cytoskeletal protein HMW2 and cytadherence." Krause D.C., Proft T., Hedreyda C.T., Hilbert H., Plagens H., Herrmann R. J. Bacteriol. 179:2668-2677(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29342 / M129.
[3]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[4]	"Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins." Proft T., Herrmann R. Mol. Microbiol. 13:337-348(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-75 AND 83-95, SUBCELLULAR LOCATION. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34977 Genomic DNA. Translation: CAA84429.1. U59896 Genomic DNA. Translation: AAB52526.1. U00089 Genomic DNA. Translation: AAB96175.1. Z32653 Genomic DNA. Translation: CAA83574.1. Z32655 Genomic DNA. Translation: CAA83576.1.
PIR	S73853.
RefSeq	NP_109997.1. NC_000912.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96175; AAB96175; MPN_309.
GeneID	876932.
KEGG	mpn:MPN309.
PATRIC	20021970. VBIMycPne110_0333.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-325-MONOMER.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

P65_MYCPN

Accession

Primary (citable) accession number: P0CJ81
Secondary accession number(s): P53663 , P53664, Q6LAC2, Q6LAC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program