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Protein

Chlorophyll a-b binding protein 2, chloroplastic

Gene

LHCB1.1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity1
Binding sitei80Chlorophyll-a 1; via amide nitrogenBy similarity1
Binding sitei86Chlorophyll-a 1By similarity1
Metal bindingi99Magnesium (chlorophyll-a 1 axial ligand)By similarity1
Metal bindingi102Magnesium (chlorophyll-a 2 axial ligand)By similarity1
Binding sitei104Chlorophyll-b 2By similarity1
Binding sitei137Chlorophyll-a 3By similarity1
Binding sitei147Chlorophyll-a 3; via amide nitrogenBy similarity1
Metal bindingi153Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygenBy similarity1
Binding sitei157Chlorophyll-b 3By similarity1
Binding sitei165Chlorophyll-b 4 or chlorophyll-b 5By similarity1
Metal bindingi173Magnesium (chlorophyll-b 3 axial ligand)By similarity1
Binding sitei176Chlorophyll-b 4By similarity1
Binding sitei183Chlorophyll-b 2; via amide nitrogenBy similarity1
Binding sitei214Chlorophyll-a 5By similarity1
Metal bindingi215Magnesium (chlorophyll-a 3 axial ligand)By similarity1
Metal bindingi218Magnesium (chlorophyll-a 4 axial ligand)By similarity1
Binding sitei220Chlorophyll-a 1By similarity1
Metal bindingi232Magnesium (chlorophyll-a 5 axial ligand)By similarity1
Metal bindingi247Magnesium (chlorophyll-a 6 axial ligand)By similarity1
Binding sitei256Chlorophyll-a 6; via amide nitrogenBy similarity1
Binding sitei263Chlorophyll-b 5; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • chlorophyll binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • pigment binding Source: GO_Central

GO - Biological processi

  • photosynthesis, light harvesting in photosystem I Source: GO_Central
  • protein-chromophore linkage Source: UniProtKB-KW
  • response to blue light Source: TAIR
  • response to far red light Source: TAIR
  • response to fructose Source: TAIR
  • response to red light Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein 2, chloroplastic
Alternative name(s):
Chlorophyll a-b protein 165
Short name:
CAB-165
LHCII type I CAB-2
Gene namesi
Name:LHCB1.1
Synonyms:AB165, CAB2, LHCP-B
Ordered Locus Names:At1g29920
ORF Names:F1N18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G29920.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Transmembranei221 – 241HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: GO_Central
  • chloroplast thylakoid membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • photosystem I Source: UniProtKB-KW
  • photosystem II Source: UniProtKB-KW
  • plastoglobule Source: GO_Central
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Photosystem II, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197 – 200DPLG → AAAA: Loss of interaction with CAO/cpSRP43, but no effect on interaction with LTD. 1 Publication4
Mutagenesisi199L → K: Loss of targeting to the thylakoid. 1 Publication1
Mutagenesisi210 – 212ELK → AAA: Loss of interaction with LTD, but no effect on interaction with CAO/cpSRP43. 1 Publication3
Mutagenesisi220 – 223RLAM → AAAA: Loss of interaction with LTD, but no effect on interaction with CAO/cpSRP43. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35Chloroplast1 PublicationAdd BLAST35
ChainiPRO_000000364736 – 267Chlorophyll a-b binding protein 2, chloroplasticAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36N2-acetylarginine1 Publication1
Modified residuei38Phosphothreonine1 Publication1

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP0CJ48.
PRIDEiP0CJ48.

PTM databases

iPTMnetiP0CJ48.

Expressioni

Gene expression databases

ExpressionAtlasiP0CJ48. baseline and differential.
GenevisibleiP0CJ48. AT.

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins. Interacts (via T14 domain) with LTD. Interacts with CAO/cpSRP43 during its post-translational targeting to the thylakoid.2 Publications

Protein-protein interaction databases

BioGridi25104. 14 interactors.
25105. 10 interactors.
IntActiP0CJ48. 1 interactor.
STRINGi3702.AT1G29920.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YZHX-ray2.00B36-50[»]
ProteinModelPortaliP0CJ48.
SMRiP0CJ48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 224Interaction with LTD1 Publication4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi197 – 200Required for interaction with CAO/cpSRP434

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.
The L18 domain (188-205) is required for the transit complex formation with CAO/cpSRP43 and the targeting to the thylakoid.
The T14 domain (211-224) is required for the interaction with LTD and the translocation across the envelope.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IU0X. Eukaryota.
ENOG410ZHBU. LUCA.
HOGENOMiHOG000238032.
InParanoidiP0CJ48.
KOiK08912.
OrthoDBiEOG09360I0J.
PhylomeDBiP0CJ48.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CJ48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASTMALSS PAFAGKAVNL SPAASEVLGS GRVTMRKTVA KPKGPSGSPW
60 70 80 90 100
YGSDRVKYLG PFSGESPSYL TGEFPGDYGW DTAGLSADPE TFARNRELEV
110 120 130 140 150
IHSRWAMLGA LGCVFPELLA RNGVKFGEAV WFKAGSQIFS DGGLDYLGNP
160 170 180 190 200
SLVHAQSILA IWATQVILMG AVEGYRVAGN GPLGEAEDLL YPGGSFDPLG
210 220 230 240 250
LATDPEAFAE LKVKELKNGR LAMFSMFGFF VQAIVTGKGP IENLADHLAD
260
PVNNNAWAFA TNFVPGK
Length:267
Mass (Da):28,227
Last modified:January 11, 2011 - v1
Checksum:iCEF0664E98F0B0BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03907 Genomic DNA. Translation: CAA27540.1.
AC008030 Genomic DNA. Translation: AAG10604.1.
CP002684 Genomic DNA. Translation: AEE31150.1.
AY052237 mRNA. Translation: AAK97707.1.
AY054198 mRNA. Translation: AAL06859.1.
AY060500 mRNA. Translation: AAL31113.1.
AY062814 mRNA. Translation: AAL32892.1.
AY081572 mRNA. Translation: AAM10134.1.
AY128345 mRNA. Translation: AAM91548.1.
BT000726 mRNA. Translation: AAN31868.1.
AY086905 mRNA. Translation: AAM63949.1.
PIRiA29280.
RefSeqiNP_564339.1. NM_102731.3.
NP_564340.1. NM_102732.3.
UniGeneiAt.10812.
At.24351.
At.27031.
At.32199.
At.43708.
At.49175.
At.66422.
At.70617.
At.71449.
At.71528.
At.71547.

Genome annotation databases

EnsemblPlantsiAT1G29910.1; AT1G29910.1; AT1G29910.
AT1G29920.1; AT1G29920.1; AT1G29920.
GeneIDi839869.
839870.
GrameneiAT1G29910.1; AT1G29910.1; AT1G29910.
AT1G29920.1; AT1G29920.1; AT1G29920.
KEGGiath:AT1G29910.
ath:AT1G29920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03907 Genomic DNA. Translation: CAA27540.1.
AC008030 Genomic DNA. Translation: AAG10604.1.
CP002684 Genomic DNA. Translation: AEE31150.1.
AY052237 mRNA. Translation: AAK97707.1.
AY054198 mRNA. Translation: AAL06859.1.
AY060500 mRNA. Translation: AAL31113.1.
AY062814 mRNA. Translation: AAL32892.1.
AY081572 mRNA. Translation: AAM10134.1.
AY128345 mRNA. Translation: AAM91548.1.
BT000726 mRNA. Translation: AAN31868.1.
AY086905 mRNA. Translation: AAM63949.1.
PIRiA29280.
RefSeqiNP_564339.1. NM_102731.3.
NP_564340.1. NM_102732.3.
UniGeneiAt.10812.
At.24351.
At.27031.
At.32199.
At.43708.
At.49175.
At.66422.
At.70617.
At.71449.
At.71528.
At.71547.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YZHX-ray2.00B36-50[»]
ProteinModelPortaliP0CJ48.
SMRiP0CJ48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25104. 14 interactors.
25105. 10 interactors.
IntActiP0CJ48. 1 interactor.
STRINGi3702.AT1G29920.1.

PTM databases

iPTMnetiP0CJ48.

Proteomic databases

PaxDbiP0CJ48.
PRIDEiP0CJ48.

Protocols and materials databases

DNASUi839869.
839870.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G29910.1; AT1G29910.1; AT1G29910.
AT1G29920.1; AT1G29920.1; AT1G29920.
GeneIDi839869.
839870.
GrameneiAT1G29910.1; AT1G29910.1; AT1G29910.
AT1G29920.1; AT1G29920.1; AT1G29920.
KEGGiath:AT1G29910.
ath:AT1G29920.

Organism-specific databases

TAIRiAT1G29920.

Phylogenomic databases

eggNOGiENOG410IU0X. Eukaryota.
ENOG410ZHBU. LUCA.
HOGENOMiHOG000238032.
InParanoidiP0CJ48.
KOiK08912.
OrthoDBiEOG09360I0J.
PhylomeDBiP0CJ48.

Miscellaneous databases

PROiP0CJ48.

Gene expression databases

ExpressionAtlasiP0CJ48. baseline and differential.
GenevisibleiP0CJ48. AT.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCB1A_ARATH
AccessioniPrimary (citable) accession number: P0CJ48
Secondary accession number(s): P04777, P83754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.