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P0CJ48

- CB1A_ARATH

UniProt

P0CJ48 - CB1A_ARATH

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Protein
Chlorophyll a-b binding protein 2, chloroplastic
Gene
LHCB1.1, AB165, CAB2, LHCP-B, At1g29920, F1N18.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.

Cofactori

Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygen By similarity
Binding sitei80 – 801Chlorophyll-a 1; via amide nitrogen By similarity
Binding sitei86 – 861Chlorophyll-a 1 By similarity
Metal bindingi99 – 991Magnesium (chlorophyll-a 1 axial ligand) By similarity
Metal bindingi102 – 1021Magnesium (chlorophyll-a 2 axial ligand) By similarity
Binding sitei104 – 1041Chlorophyll-b 2 By similarity
Binding sitei137 – 1371Chlorophyll-a 3 By similarity
Binding sitei147 – 1471Chlorophyll-a 3; via amide nitrogen By similarity
Metal bindingi153 – 1531Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygen By similarity
Binding sitei157 – 1571Chlorophyll-b 3 By similarity
Binding sitei165 – 1651Chlorophyll-b 4 or chlorophyll-b 5 By similarity
Metal bindingi173 – 1731Magnesium (chlorophyll-b 3 axial ligand) By similarity
Binding sitei176 – 1761Chlorophyll-b 4 By similarity
Binding sitei183 – 1831Chlorophyll-b 2; via amide nitrogen By similarity
Binding sitei214 – 2141Chlorophyll-a 5 By similarity
Metal bindingi215 – 2151Magnesium (chlorophyll-a 3 axial ligand) By similarity
Metal bindingi218 – 2181Magnesium (chlorophyll-a 4 axial ligand) By similarity
Binding sitei220 – 2201Chlorophyll-a 1 By similarity
Metal bindingi232 – 2321Magnesium (chlorophyll-a 5 axial ligand) By similarity
Metal bindingi247 – 2471Magnesium (chlorophyll-a 6 axial ligand) By similarity
Binding sitei256 – 2561Chlorophyll-a 6; via amide nitrogen By similarity
Binding sitei263 – 2631Chlorophyll-b 5; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. chlorophyll binding Source: TAIR
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. photosynthesis, light harvesting Source: InterPro
  2. protein-chromophore linkage Source: UniProtKB-KW
  3. response to blue light Source: TAIR
  4. response to far red light Source: TAIR
  5. response to fructose Source: TAIR
  6. response to red light Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein 2, chloroplastic
Alternative name(s):
Chlorophyll a-b protein 165
Short name:
CAB-165
LHCII type I CAB-2
Gene namesi
Name:LHCB1.1
Synonyms:AB165, CAB2, LHCP-B
Ordered Locus Names:At1g29920
ORF Names:F1N18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G29920.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei100 – 12021Helical; Reviewed prediction
Add
BLAST
Transmembranei152 – 17221Helical; Reviewed prediction
Add
BLAST
Transmembranei221 – 24121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast thylakoid membrane Source: TAIR
  4. integral component of membrane Source: UniProtKB-KW
  5. photosystem I Source: UniProtKB-KW
  6. photosystem II Source: UniProtKB-KW
  7. thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Photosystem II, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi197 – 2004DPLG → AAAA: Loss of interaction with CAO/cpSRP43, but no effect on interaction with LTD.
Mutagenesisi199 – 1991L → K: Loss of targeting to the thylakoid. 1 Publication
Mutagenesisi210 – 2123ELK → AAA: Loss of interaction with LTD, but no effect on interaction with CAO/cpSRP43. 1 Publication
Mutagenesisi220 – 2234RLAM → AAAA: Loss of interaction with LTD, but no effect on interaction with CAO/cpSRP43. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535Chloroplast1 Publication
Add
BLAST
Chaini36 – 267232Chlorophyll a-b binding protein 2, chloroplastic
PRO_0000003647Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361N2-acetylarginine
Modified residuei38 – 381Phosphothreonine1 Publication

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP0CJ48.

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins. Interacts (via T14 domain) with LTD. Interacts with CAO/cpSRP43 during its post-translational targeting to the thylakoid.2 Publications

Protein-protein interaction databases

BioGridi25104. 13 interactions.
25105. 8 interactions.
IntActiP0CJ48. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP0CJ48.
SMRiP0CJ48. Positions 48-267.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 2244Interaction with LTD

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi197 – 2004Required for interaction with CAO/cpSRP43

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.
The L18 domain (188-205) is required for the transit complex formation with CAO/cpSRP43 and the targeting to the thylakoid.
The T14 domain (211-224) is required for the interaction with LTD and the translocation across the envelope.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000238032.
KOiK08912.
PhylomeDBiP0CJ48.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CJ48-1 [UniParc]FASTAAdd to Basket

« Hide

MAASTMALSS PAFAGKAVNL SPAASEVLGS GRVTMRKTVA KPKGPSGSPW    50
YGSDRVKYLG PFSGESPSYL TGEFPGDYGW DTAGLSADPE TFARNRELEV 100
IHSRWAMLGA LGCVFPELLA RNGVKFGEAV WFKAGSQIFS DGGLDYLGNP 150
SLVHAQSILA IWATQVILMG AVEGYRVAGN GPLGEAEDLL YPGGSFDPLG 200
LATDPEAFAE LKVKELKNGR LAMFSMFGFF VQAIVTGKGP IENLADHLAD 250
PVNNNAWAFA TNFVPGK 267
Length:267
Mass (Da):28,227
Last modified:January 11, 2011 - v1
Checksum:iCEF0664E98F0B0BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03907 Genomic DNA. Translation: CAA27540.1.
AC008030 Genomic DNA. Translation: AAG10604.1.
CP002684 Genomic DNA. Translation: AEE31150.1.
AY052237 mRNA. Translation: AAK97707.1.
AY054198 mRNA. Translation: AAL06859.1.
AY060500 mRNA. Translation: AAL31113.1.
AY062814 mRNA. Translation: AAL32892.1.
AY081572 mRNA. Translation: AAM10134.1.
AY128345 mRNA. Translation: AAM91548.1.
BT000726 mRNA. Translation: AAN31868.1.
AY086905 mRNA. Translation: AAM63949.1.
PIRiA29280.
RefSeqiNP_564339.1. NM_102731.2.
NP_564340.1. NM_102732.2.
UniGeneiAt.10812.
At.24351.
At.27031.
At.32199.
At.43708.
At.49175.
At.66422.
At.70617.
At.71449.
At.71528.
At.71547.

Genome annotation databases

EnsemblPlantsiAT1G29910.1; AT1G29910.1; AT1G29910.
AT1G29920.1; AT1G29920.1; AT1G29920.
GeneIDi839869.
839870.
KEGGiath:AT1G29910.
ath:AT1G29920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03907 Genomic DNA. Translation: CAA27540.1 .
AC008030 Genomic DNA. Translation: AAG10604.1 .
CP002684 Genomic DNA. Translation: AEE31150.1 .
AY052237 mRNA. Translation: AAK97707.1 .
AY054198 mRNA. Translation: AAL06859.1 .
AY060500 mRNA. Translation: AAL31113.1 .
AY062814 mRNA. Translation: AAL32892.1 .
AY081572 mRNA. Translation: AAM10134.1 .
AY128345 mRNA. Translation: AAM91548.1 .
BT000726 mRNA. Translation: AAN31868.1 .
AY086905 mRNA. Translation: AAM63949.1 .
PIRi A29280.
RefSeqi NP_564339.1. NM_102731.2.
NP_564340.1. NM_102732.2.
UniGenei At.10812.
At.24351.
At.27031.
At.32199.
At.43708.
At.49175.
At.66422.
At.70617.
At.71449.
At.71528.
At.71547.

3D structure databases

ProteinModelPortali P0CJ48.
SMRi P0CJ48. Positions 48-267.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 25104. 13 interactions.
25105. 8 interactions.
IntActi P0CJ48. 1 interaction.

Proteomic databases

PRIDEi P0CJ48.

Protocols and materials databases

DNASUi 839869.
839870.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G29910.1 ; AT1G29910.1 ; AT1G29910 .
AT1G29920.1 ; AT1G29920.1 ; AT1G29920 .
GeneIDi 839869.
839870.
KEGGi ath:AT1G29910.
ath:AT1G29920.

Organism-specific databases

TAIRi AT1G29920.

Phylogenomic databases

HOGENOMi HOG000238032.
KOi K08912.
PhylomeDBi P0CJ48.

Family and domain databases

Gene3Di 1.10.3460.10. 1 hit.
InterProi IPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view ]
PANTHERi PTHR21649. PTHR21649. 1 hit.
Pfami PF00504. Chloroa_b-bind. 1 hit.
[Graphical view ]
SUPFAMi SSF103511. SSF103511. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana."
    Leutwiler L.S., Meyerowitz E.M., Tobin E.M.
    Nucleic Acids Res. 14:4051-4064(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Mass spectrometric resolution of reversible protein phosphorylation in photosynthetic membranes of Arabidopsis thaliana."
    Vener A.V., Harms A., Sussman M.R., Vierstra R.D.
    J. Biol. Chem. 276:6959-6966(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-43, IDENTIFICATION BY MASS SPECTROMETRY, PHOSPHORYLATION AT THR-38, ACETYLATION AT ARG-36.
    Strain: cv. Columbia.
  7. "Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43."
    Stengel K.F., Holdermann I., Cain P., Robinson C., Wild K., Sinning I.
    Science 321:253-256(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CAO/CPSRP43, MUTAGENESIS OF LEU-199.
  8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway."
    Ouyang M., Li X., Ma J., Chi W., Xiao J., Zou M., Chen F., Lu C., Zhang L.
    Nat. Commun. 2:277-277(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LTD, MUTAGENESIS OF 197-ASP--GLY-200; 210-GLU--LYS-212 AND 220-ARG--MET-223.

Entry informationi

Entry nameiCB1A_ARATH
AccessioniPrimary (citable) accession number: P0CJ48
Secondary accession number(s): P04777, P83754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi