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Protein

Actin-3

Gene

ACT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the reproductive actins.

Miscellaneous

There are 8 actin genes in A.thaliana.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-ATH-5663213. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-3
Gene namesi
Name:ACT3
Ordered Locus Names:At3g53750
ORF Names:F5K20_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G53750.
TAIRilocus:2084410. AT3G53750.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • nucleus Source: TAIR
  • plasmodesma Source: TAIR

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004039372 – 377Actin-3Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP0CJ47.
PRIDEiP0CJ47.

Expressioni

Tissue specificityi

Preferentially expressed in mature pollen, pollen tubes, young embryo sac, and organ primordia. Little or no reproductive-gene expression is detected in vegetative organs, such as root, stems, leaves, sepals and petals.

Gene expression databases

ExpressionAtlasiP0CJ47. baseline and differential.
GenevisibleiP0CJ47. AT.

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Protein-protein interaction databases

BioGridi3683. 8 interactors.
9859. 2 interactors.
IntActiP0CJ47. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliP0CJ47.
SMRiP0CJ47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
InParanoidiP0CJ47.
KOiK10355.
OrthoDBiEOG09360BTG.
PhylomeDBiP0CJ47.

Family and domain databases

InterProiView protein in InterPro
IPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiView protein in Pfam
PF00022. Actin. 1 hit.
PRINTSiPR00190. ACTIN.
SMARTiView protein in SMART
SM00268. ACTIN. 1 hit.
PROSITEiView protein in PROSITE
PS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CJ47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP
110 120 130 140 150
EEHPILLTEA PLNPKANREK MTQIMFETFN APAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDA LMKILTERGY
210 220 230 240 250
SFTTTAEREI VRDIKEKLCY IALDYEQELE TAKTSSSVEK NYELPDGQVI
260 270 280 290 300
TIGSERFRCP EVLYQPSMIG MENAGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGTTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIA KAEYDESGPS IVHRKCF
Length:377
Mass (Da):41,798
Last modified:January 11, 2011 - v1
Checksum:i44D749AD5A9671BB
GO

Sequence cautioni

The sequence CAB88337 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39480 Genomic DNA. No translation available.
AL132960 Genomic DNA. Translation: CAB88337.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79138.1.
CP002686 Genomic DNA. Translation: ANM64878.1.
AY049293 mRNA. Translation: AAK83635.1.
BT002257 mRNA. Translation: AAN72268.1.
AY086557 mRNA. Translation: AAM63620.1.
PIRiS68112.
RefSeqiNP_001031504.1. NM_001036427.3.
NP_001324518.1. NM_001336664.1.
NP_001324519.1. NM_001336665.1.
NP_001326881.1. NM_001339629.1.
NP_566988.1. NM_115235.4.
NP_850284.1. NM_179953.3.
UniGeneiAt.11577.
At.23346.
At.23663.
At.23817.

Genome annotation databases

EnsemblPlantsiAT2G37620.1; AT2G37620.1; AT2G37620.
AT2G37620.2; AT2G37620.2; AT2G37620.
AT2G37620.3; AT2G37620.3; AT2G37620.
AT2G37620.4; AT2G37620.4; AT2G37620.
AT3G53750.1; AT3G53750.1; AT3G53750.
AT3G53750.2; AT3G53750.2; AT3G53750.
GeneIDi818339.
824542.
GrameneiAT2G37620.1; AT2G37620.1; AT2G37620.
AT2G37620.2; AT2G37620.2; AT2G37620.
AT2G37620.3; AT2G37620.3; AT2G37620.
AT2G37620.4; AT2G37620.4; AT2G37620.
AT3G53750.1; AT3G53750.1; AT3G53750.
AT3G53750.2; AT3G53750.2; AT3G53750.
KEGGiath:AT2G37620.
ath:AT3G53750.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiACT3_ARATH
AccessioniPrimary (citable) accession number: P0CJ47
Secondary accession number(s): P10671, P53493, Q9M351
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: July 5, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families