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P0CJ44 (ALF_COCIM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative fructose-bisphosphate aldolase

Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13
Alternative name(s):
Putative fructose-1,6-bisphosphate aldolase
Gene names
ORF Names:CIMG_05755
OrganismCoccidioides immitis (strain RS) (Valley fever fungus) [Reference proteome]
Taxonomic identifier246410 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesmitosporic OnygenalesCoccidioides

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactor

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the class II fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Putative fructose-bisphosphate aldolase
PRO_0000403787

Regions

Region224 – 2263Dihydroxyacetone phosphate binding By similarity
Region245 – 2484Dihydroxyacetone phosphate binding By similarity

Sites

Active site861Proton donor By similarity
Metal binding871Zinc 1; catalytic By similarity
Metal binding1161Zinc 2 By similarity
Metal binding1461Zinc 2 By similarity
Metal binding1921Zinc 1; catalytic By similarity
Metal binding2231Zinc 1; catalytic By similarity
Binding site1931Dihydroxyacetone phosphate; via amide nitrogen By similarity

Secondary structure

............................................. 302
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0CJ44 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: 3FBF318A0A3421C6

FASTA30233,389
        10         20         30         40         50         60 
MPHPSLKSNR ALPLLTFART HSFAIPAICV YNLEGILAII RAAEHKRSPA MILLFPWAIQ 

        70         80         90        100        110        120 
YADSLLVRTA ASACRAASVP ITLHLDHAQD PEIIKRAADL SRSETHEPGF DSIMVDMSHF 

       130        140        150        160        170        180 
SKEENLRLTR ELVAYCNARG IATEAEPGRI EGGEDGVQDT VDLEGVLTTP EESEEFVATG 

       190        200        210        220        230        240 
INWLAPAFGN VHGNYGPRGV QLDYERLQRI NEAVGERVGL VLHGADPFTK EIFEKCIERG 

       250        260        270        280        290        300 
VAKVNVNRAV NNEYVKVMRE KAGSLPITRL HEEVTNAMQA AVEKIMDMID STGKAEFMMD 


EK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GG704912 Genomic DNA. Translation: EAS30503.2.
RefSeqXP_001241859.1. XM_001241858.1.
XP_001242086.1. XM_001242085.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3PM6X-ray2.20A/B1-302[»]
ProteinModelPortalP0CJ44.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4561635.
4561677.
KEGGcim:CIMG_05755.
cim:CIMG_05982.

Phylogenomic databases

KOK01624.
K13336.
OrthoDBEOG79GTHR.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFPIRSF001359. F_bP_aldolase_II. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0CJ44.

Entry information

Entry nameALF_COCIM
AccessionPrimary (citable) accession number: P0CJ44
Secondary accession number(s): J3K765
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: June 11, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways