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P0CI79 (TYSY1_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase 1

Short name=TS 1
Short name=TSase 1
EC=2.1.1.45
Alternative name(s):
Thymidylate synthase A
Short name=TS A
Short name=TSase A
Gene names
Name:thyA1
Ordered Locus Names:BSU17680
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Miscellaneous

B.subtilis strain 168 possesses two thymidylate synthases, a major form ThyA and a minor form ThyB.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Biophysicochemical properties

Temperature dependence:

Thermostable. Active at 46 degrees Celsius. HAMAP-Rule MF_00008

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Thymidylate synthase 1 HAMAP-Rule MF_00008
PRO_0000140931

Sites

Active site1611 By similarity

Natural variations

Natural variant84 – 852ND → TE in strain: ATCC 6633.
Natural variant881M → K in strain: ATCC 6633.
Natural variant1181S → N in strain: ATCC 6633.
Natural variant1551A → S in strain: ATCC 6633.
Natural variant1711H → Q in strain: ATCC 6633.

Secondary structure

....................................................... 279
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0CI79 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: CC0B127AAF7912BD

FASTA27932,807
        10         20         30         40         50         60 
MTQFDKQYNS IIKDIINNGI SDEEFDVRTK WDSDGTPAHT LSVISKQMRF DNSEVPILTT 

        70         80         90        100        110        120 
KKVAWKTAIK ELLWIWQLKS NDVNDLNMMG VHIWDQWKQE DGTIGHAYGF QLGKKNRSLN 

       130        140        150        160        170        180 
GEKVDQVDYL LHQLKNNPSS RRHITMLWNP DELDAMALTP CVYETQWYVK HGKLHLEVRA 

       190        200        210        220        230        240 
RSNDMALGNP FNVFQYNVLQ RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF 

       250        260        270 
EAPELWINPE VKDFYDFTID DFKLINYKHG DKLLFEVAV 

« Hide

References

« Hide 'large scale' references
[1]"The thyA gene from Bacillus subtilis exhibits similarity with the phage phi 3T thymidylate synthase gene."
Tam N.H., Borriss R.
Microbiology 141:291-297(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families."
Tam N.H., Borriss R.
Mol. Gen. Genet. 258:427-430(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-279.
Strain: ATCC 6633 / NCIMB 8054 / CCM1999.
[4]"Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis."
Stout T.J., Schellenberger U., Santi D.V., Stroud R.M.
Biochemistry 37:14736-14747(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[5]"Crystal structure of thymidylate synthase A from Bacillus subtilis."
Fox K.M., Maley F., Garibian A., Changchien L.M., van Roey P.
Protein Sci. 8:538-544(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78560 Genomic DNA. Translation: CAA55307.1.
AL009126 Genomic DNA. Translation: CAB13652.1.
AF004102 Genomic DNA. Translation: AAC26324.1.
PIRI40494.
RefSeqNP_389651.1. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B02X-ray2.50A1-279[»]
1BKOX-ray2.75A/B/C/D2-279[»]
1BKPX-ray1.70A/B2-279[»]
1BSFX-ray2.20A/B2-279[»]
1BSPX-ray2.50A/B2-279[»]
ProteinModelPortalP0CI79.
SMRP0CI79. Positions 2-279.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13652; CAB13652; BSU17680.
GeneID939555.
KEGGbsu:BSU17680.
PATRIC18975359. VBIBacSub10457_1871.

Organism-specific databases

GenoListBSU17680. [Micado]

Phylogenomic databases

HOGENOMHOG000257898.
KOK00560.
OMATIGHAYG.
OrthoDBEOG6K6V53.
PhylomeDBP0CI79.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0CI79.

Entry information

Entry nameTYSY1_BACSU
AccessionPrimary (citable) accession number: P0CI79
Secondary accession number(s): O30395, O30396, P42326
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList