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P0CI75 (BIRA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional protein BirA

Including the following 2 domains:

  1. Biotin operon repressor
  2. Biotin--[acetyl-CoA-carboxylase] synthetase
    EC=6.3.4.15
    Alternative name(s):
    Biotin--protein ligase
Gene names
Name:birA
Ordered Locus Names:BSU22440
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins By similarity.

Catalytic activity

ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].

Sequence similarities

Belongs to the biotin--protein ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Bifunctional protein BirA
PRO_0000064931

Regions

DNA binding23 – 4220H-T-H motif Potential

Experimental info

Mutagenesis231G → S: Deregulation of biotin synthesis. Ref.1
Mutagenesis381W → R: Deregulation of biotin synthesis. Ref.1
Mutagenesis581G → E: Deregulation of biotin synthesis. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0CI75 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: 69088BB2CAD09221

FASTA32536,182
        10         20         30         40         50         60 
MRSTLRKDLI ELFSQAGNEF ISGQKISDAL GCSRTAVWKH IEELRKEGYE VEAVRRKGYR 

        70         80         90        100        110        120 
LIKKPGKLSE SEIRFGLKTE VMGQHLIYHD VLSSTQKTAH ELANNNAPEG TLVVADKQTA 

       130        140        150        160        170        180 
GRGRMSRVWH SQEGNGVWMS LILRPDIPLQ KTPQLTLLAA VAVVQGIEEA AGIQTDIKWP 

       190        200        210        220        230        240 
NDILINGKKT VGILTEMQAE EDRVRSVIIG IGINVNQQPN DFPDELKDIA TSLSQAAGEK 

       250        260        270        280        290        300 
IDRAGVIQHI LLCFEKRYRD YMTHGFTPIK LLWESYALGI GTNMRARTLN GTFYGKALGI 

       310        320 
DDEGVLLLET NEGIKKIYSA DIELG

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon."
Bower S., Perkins J.P., Yocum R.R., Serror P., Sorokin A.V., Rahaim P., Howitt C.L., Prasad N., Ehrlich S.D., Pero J.
J. Bacteriol. 177:2572-2575(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-23; TRP-38 AND GLY-58.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L38424 Genomic DNA. Translation: AAA92879.1. Sequence problems.
L47709 Genomic DNA. Translation: AAB38447.1.
AL009126 Genomic DNA. Translation: CAB14160.1.
PIRA69595.
RefSeqNP_390125.1. NC_000964.3.

3D structure databases

ProteinModelPortalP0CI75.
SMRP0CI75. Positions 3-324.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14160; CAB14160; BSU22440.
GeneID939028.
KEGGbsu:BSU22440.
PATRIC18976297. VBIBacSub10457_2339.

Organism-specific databases

GenoListBSU22440. [Micado]

Phylogenomic databases

HOGENOMHOG000041811.
KOK03524.
OMAAVWKHIE.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR004408. Biotin_CoA_COase_ligase.
IPR004409. Biotin_operon_repress_HTH.
IPR003142. BPL_C.
IPR004143. BPL_LipA_LipB.
IPR013196. HTH_11.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR12835. PTHR12835. 1 hit.
PfamPF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF08279. HTH_11. 1 hit.
[Graphical view]
TIGRFAMsTIGR00121. birA_ligase. 1 hit.
TIGR00122. birA_repr_reg. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIRA_BACSU
AccessionPrimary (citable) accession number: P0CI75
Secondary accession number(s): P42975
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents