Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CI75

- BIRA_BACSU

UniProt

P0CI75 - BIRA_BACSU

Protein

Bifunctional ligase/repressor BirA

Gene

birA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (11 Jan 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.UniRule annotation

    Catalytic activityi

    ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181BiotinUniRule annotation
    Binding sitei189 – 1891BiotinUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi23 – 4220H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. biotin-[acetyl-CoA-carboxylase] ligase activity Source: UniProtKB-EC
    3. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular protein modification process Source: InterPro
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Biotin, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional ligase/repressor BirAUniRule annotation
    Alternative name(s):
    Biotin--[acetyl-CoA-carboxylase] ligaseUniRule annotation (EC:6.3.4.15UniRule annotation)
    Biotin--protein ligaseUniRule annotation
    Biotin-[acetyl-CoA carboxylase] synthetaseUniRule annotation
    Gene namesi
    Name:birAUniRule annotation
    Ordered Locus Names:BSU22440
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU22440. [Micado]

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi23 – 231G → S: Deregulation of biotin synthesis. 1 Publication
    Mutagenesisi38 – 381W → R: Deregulation of biotin synthesis. 1 Publication
    Mutagenesisi58 – 581G → E: Deregulation of biotin synthesis. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Bifunctional ligase/repressor BirAPRO_0000064931Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0CI75.
    SMRiP0CI75. Positions 3-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni122 – 1243Biotin bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the biotin--protein ligase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000041811.
    KOiK03524.
    OMAiADVCHVV.
    OrthoDBiEOG6DNTBX.
    PhylomeDBiP0CI75.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    HAMAPiMF_00978. Bifunct_BirA.
    InterProiIPR004408. Biotin_CoA_COase_ligase.
    IPR004409. Biotin_operon_repress_HTH.
    IPR003142. BPL_C.
    IPR004143. BPL_LipA_LipB.
    IPR013196. HTH_11.
    IPR008988. Transcriptional_repressor_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12835. PTHR12835. 1 hit.
    PfamiPF02237. BPL_C. 1 hit.
    PF03099. BPL_LplA_LipB. 1 hit.
    PF08279. HTH_11. 1 hit.
    [Graphical view]
    SUPFAMiSSF50037. SSF50037. 1 hit.
    TIGRFAMsiTIGR00121. birA_ligase. 1 hit.
    TIGR00122. birA_repr_reg. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0CI75-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSTLRKDLI ELFSQAGNEF ISGQKISDAL GCSRTAVWKH IEELRKEGYE    50
    VEAVRRKGYR LIKKPGKLSE SEIRFGLKTE VMGQHLIYHD VLSSTQKTAH 100
    ELANNNAPEG TLVVADKQTA GRGRMSRVWH SQEGNGVWMS LILRPDIPLQ 150
    KTPQLTLLAA VAVVQGIEEA AGIQTDIKWP NDILINGKKT VGILTEMQAE 200
    EDRVRSVIIG IGINVNQQPN DFPDELKDIA TSLSQAAGEK IDRAGVIQHI 250
    LLCFEKRYRD YMTHGFTPIK LLWESYALGI GTNMRARTLN GTFYGKALGI 300
    DDEGVLLLET NEGIKKIYSA DIELG 325
    Length:325
    Mass (Da):36,182
    Last modified:January 11, 2011 - v1
    Checksum:i69088BB2CAD09221
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38424 Genomic DNA. Translation: AAA92879.1. Sequence problems.
    L47709 Genomic DNA. Translation: AAB38447.1.
    AL009126 Genomic DNA. Translation: CAB14160.1.
    PIRiA69595.
    RefSeqiNP_390125.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14160; CAB14160; BSU22440.
    GeneIDi939028.
    KEGGibsu:BSU22440.
    PATRICi18976297. VBIBacSub10457_2339.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38424 Genomic DNA. Translation: AAA92879.1 . Sequence problems.
    L47709 Genomic DNA. Translation: AAB38447.1 .
    AL009126 Genomic DNA. Translation: CAB14160.1 .
    PIRi A69595.
    RefSeqi NP_390125.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P0CI75.
    SMRi P0CI75. Positions 3-324.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14160 ; CAB14160 ; BSU22440 .
    GeneIDi 939028.
    KEGGi bsu:BSU22440.
    PATRICi 18976297. VBIBacSub10457_2339.

    Organism-specific databases

    GenoListi BSU22440. [Micado ]

    Phylogenomic databases

    HOGENOMi HOG000041811.
    KOi K03524.
    OMAi ADVCHVV.
    OrthoDBi EOG6DNTBX.
    PhylomeDBi P0CI75.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    HAMAPi MF_00978. Bifunct_BirA.
    InterProi IPR004408. Biotin_CoA_COase_ligase.
    IPR004409. Biotin_operon_repress_HTH.
    IPR003142. BPL_C.
    IPR004143. BPL_LipA_LipB.
    IPR013196. HTH_11.
    IPR008988. Transcriptional_repressor_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12835. PTHR12835. 1 hit.
    Pfami PF02237. BPL_C. 1 hit.
    PF03099. BPL_LplA_LipB. 1 hit.
    PF08279. HTH_11. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50037. SSF50037. 1 hit.
    TIGRFAMsi TIGR00121. birA_ligase. 1 hit.
    TIGR00122. birA_repr_reg. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon."
      Bower S., Perkins J.P., Yocum R.R., Serror P., Sorokin A.V., Rahaim P., Howitt C.L., Prasad N., Ehrlich S.D., Pero J.
      J. Bacteriol. 177:2572-2575(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-23; TRP-38 AND GLY-58.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
      Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
      Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiBIRA_BACSU
    AccessioniPrimary (citable) accession number: P0CI75
    Secondary accession number(s): P42975
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3