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Protein

3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase

Gene

hcaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively.By similarity1 Publication

Catalytic activityi

3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+ = 3-(2,3-dihydroxyphenyl)propanoate + NADH.1 Publication
(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+ = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NADH.1 Publication

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei143SubstrateBy similarity1
Active sitei156Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 34NADBy similarityAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:PHENPRODIOLDEHYDROG-MONOMER
MetaCyc:PHENPRODIOLDEHYDROG-MONOMER
UniPathwayiUPA00714

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (EC:1.3.1.87)
Alternative name(s):
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase
CI-dihydrodiol dehydrogenase
Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
PP-dihydrodiol dehydrogenase
Gene namesi
Name:hcaB
Synonyms:phdD, yfhX
Ordered Locus Names:b2541, JW2525
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13459 hcaB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547041 – 2703-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenaseAdd BLAST270

Proteomic databases

PaxDbiP0CI31
PRIDEiP0CI31

Interactioni

Protein-protein interaction databases

BioGridi4260604, 7 interactors
DIPiDIP-9863N
IntActiP0CI31, 7 interactors
STRINGi316385.ECDH10B_2708

Structurei

3D structure databases

ProteinModelPortaliP0CI31
SMRiP0CI31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108QJ2 Bacteria
ENOG410XQCB LUCA
InParanoidiP0CI31
KOiK05711
OMAiPNAGIWD
PhylomeDBiP0CI31

Family and domain databases

HAMAPiMF_01647 HcaB, 1 hit
InterProiView protein in InterPro
IPR023643 Dihydrodiol_DH_HcaB
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

P0CI31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF
60 70 80 90 100
GEHILAVEGN VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT
110 120 130 140 150
PAETLETGFH ELFNVNVLGY LLGAKACAPA LIASEGSMIF TLSNAAWYPG
160 170 180 190 200
GGGPLYTASK HAATGLIRQL AYELAPKVRV NGVGPCGMAS DLRGPQALGQ
210 220 230 240 250
SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLTSRR NNRALSGVMI
260 270
NADAGLAIRG IRHVAAGLDL
Length:270
Mass (Da):28,500
Last modified:November 30, 2010 - v1
Checksum:i607D316E7442D3E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75594.1
AP009048 Genomic DNA Translation: BAA16444.1
PIRiD65031
RefSeqiNP_417036.1, NC_000913.3
WP_001281379.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75594; AAC75594; b2541
BAA16444; BAA16444; BAA16444
GeneIDi945346
KEGGiecj:JW2525
eco:b2541
PATRICifig|1411691.4.peg.4193

Similar proteinsi

Entry informationi

Entry nameiHCAB_ECOLI
AccessioniPrimary (citable) accession number: P0CI31
Secondary accession number(s): P76995, P77646
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: March 28, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health