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P0CI31

- HCAB_ECOLI

UniProt

P0CI31 - HCAB_ECOLI

Protein

3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase

Gene

hcaB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively.By similarity

    Catalytic activityi

    3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+ = 3-(2,3-dihydroxyphenyl)propanoate + NADH.1 Publication
    (2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+ = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NADH.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei143 – 1431SubstrateBy similarity
    Active sitei156 – 1561Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 3425NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 3-phenylpropionate catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:PHENPRODIOLDEHYDROG-MONOMER.
    MetaCyc:PHENPRODIOLDEHYDROG-MONOMER.
    UniPathwayiUPA00714.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (EC:1.3.1.87)
    Alternative name(s):
    2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
    3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase
    CI-dihydrodiol dehydrogenase
    Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
    Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
    PP-dihydrodiol dehydrogenase
    Gene namesi
    Name:hcaB
    Synonyms:phdD, yfhX
    Ordered Locus Names:b2541, JW2525
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13459. hcaB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2702703-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenasePRO_0000054704Add
    BLAST

    Proteomic databases

    PRIDEiP0CI31.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9863N.
    IntActiP0CI31. 7 interactions.
    MINTiMINT-1239788.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CI31.
    SMRiP0CI31. Positions 4-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK05711.
    OMAiTASKHAC.
    OrthoDBiEOG625JVT.
    PhylomeDBiP0CI31.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_01647. HcaB.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR023643. Dihydrodiol_DH_HcaB.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CI31-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF    50
    GEHILAVEGN VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT 100
    PAETLETGFH ELFNVNVLGY LLGAKACAPA LIASEGSMIF TLSNAAWYPG 150
    GGGPLYTASK HAATGLIRQL AYELAPKVRV NGVGPCGMAS DLRGPQALGQ 200
    SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLTSRR NNRALSGVMI 250
    NADAGLAIRG IRHVAAGLDL 270
    Length:270
    Mass (Da):28,500
    Last modified:November 30, 2010 - v1
    Checksum:i607D316E7442D3E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75594.1.
    AP009048 Genomic DNA. Translation: BAA16444.1.
    PIRiD65031.
    RefSeqiNP_417036.1. NC_000913.3.
    YP_490769.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75594; AAC75594; b2541.
    BAA16444; BAA16444; BAA16444.
    GeneIDi12931610.
    945346.
    KEGGiecj:Y75_p2494.
    eco:b2541.
    PATRICi32120479. VBIEscCol129921_2642.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75594.1 .
    AP009048 Genomic DNA. Translation: BAA16444.1 .
    PIRi D65031.
    RefSeqi NP_417036.1. NC_000913.3.
    YP_490769.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0CI31.
    SMRi P0CI31. Positions 4-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9863N.
    IntActi P0CI31. 7 interactions.
    MINTi MINT-1239788.

    Proteomic databases

    PRIDEi P0CI31.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75594 ; AAC75594 ; b2541 .
    BAA16444 ; BAA16444 ; BAA16444 .
    GeneIDi 12931610.
    945346.
    KEGGi ecj:Y75_p2494.
    eco:b2541.
    PATRICi 32120479. VBIEscCol129921_2642.

    Organism-specific databases

    EchoBASEi EB3232.
    EcoGenei EG13459. hcaB.

    Phylogenomic databases

    KOi K05711.
    OMAi TASKHAC.
    OrthoDBi EOG625JVT.
    PhylomeDBi P0CI31.

    Enzyme and pathway databases

    UniPathwayi UPA00714 .
    BioCyci EcoCyc:PHENPRODIOLDEHYDROG-MONOMER.
    MetaCyc:PHENPRODIOLDEHYDROG-MONOMER.

    Miscellaneous databases

    PROi P0CI31.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_01647. HcaB.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR023643. Dihydrodiol_DH_HcaB.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12."
      Diaz E., Ferrandez A., Garcia J.L.
      J. Bacteriol. 180:2915-2923(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CATABOLISM OF PHENYLPROPIONIC AND CINNAMIC ACIDS, CATALYTIC ACTIVITY.
      Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.

    Entry informationi

    Entry nameiHCAB_ECOLI
    AccessioniPrimary (citable) accession number: P0CI31
    Secondary accession number(s): P76995, P77646
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3