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Protein

3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase

Gene

hcaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively.By similarity

Catalytic activityi

3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+ = 3-(2,3-dihydroxyphenyl)propanoate + NADH.1 Publication
(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+ = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NADH.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431SubstrateBy similarity
Active sitei156 – 1561Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3425NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. 2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:PHENPRODIOLDEHYDROG-MONOMER.
MetaCyc:PHENPRODIOLDEHYDROG-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (EC:1.3.1.87)
Alternative name(s):
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase
CI-dihydrodiol dehydrogenase
Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
PP-dihydrodiol dehydrogenase
Gene namesi
Name:hcaB
Synonyms:phdD, yfhX
Ordered Locus Names:b2541, JW2525
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13459. hcaB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2702703-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenasePRO_0000054704Add
BLAST

Proteomic databases

PRIDEiP0CI31.

Interactioni

Protein-protein interaction databases

DIPiDIP-9863N.
IntActiP0CI31. 7 interactions.
MINTiMINT-1239788.

Structurei

3D structure databases

ProteinModelPortaliP0CI31.
SMRiP0CI31. Positions 4-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP0CI31.
KOiK05711.
OMAiPNAGIWD.
OrthoDBiEOG625JVT.
PhylomeDBiP0CI31.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01647. HcaB.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR023643. Dihydrodiol_DH_HcaB.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CI31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF
60 70 80 90 100
GEHILAVEGN VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT
110 120 130 140 150
PAETLETGFH ELFNVNVLGY LLGAKACAPA LIASEGSMIF TLSNAAWYPG
160 170 180 190 200
GGGPLYTASK HAATGLIRQL AYELAPKVRV NGVGPCGMAS DLRGPQALGQ
210 220 230 240 250
SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLTSRR NNRALSGVMI
260 270
NADAGLAIRG IRHVAAGLDL
Length:270
Mass (Da):28,500
Last modified:November 30, 2010 - v1
Checksum:i607D316E7442D3E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75594.1.
AP009048 Genomic DNA. Translation: BAA16444.1.
PIRiD65031.
RefSeqiNP_417036.1. NC_000913.3.
YP_490769.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75594; AAC75594; b2541.
BAA16444; BAA16444; BAA16444.
GeneIDi12931610.
945346.
KEGGiecj:Y75_p2494.
eco:b2541.
PATRICi32120479. VBIEscCol129921_2642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75594.1.
AP009048 Genomic DNA. Translation: BAA16444.1.
PIRiD65031.
RefSeqiNP_417036.1. NC_000913.3.
YP_490769.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0CI31.
SMRiP0CI31. Positions 4-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9863N.
IntActiP0CI31. 7 interactions.
MINTiMINT-1239788.

Proteomic databases

PRIDEiP0CI31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75594; AAC75594; b2541.
BAA16444; BAA16444; BAA16444.
GeneIDi12931610.
945346.
KEGGiecj:Y75_p2494.
eco:b2541.
PATRICi32120479. VBIEscCol129921_2642.

Organism-specific databases

EchoBASEiEB3232.
EcoGeneiEG13459. hcaB.

Phylogenomic databases

InParanoidiP0CI31.
KOiK05711.
OMAiPNAGIWD.
OrthoDBiEOG625JVT.
PhylomeDBiP0CI31.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciEcoCyc:PHENPRODIOLDEHYDROG-MONOMER.
MetaCyc:PHENPRODIOLDEHYDROG-MONOMER.

Miscellaneous databases

PROiP0CI31.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01647. HcaB.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR023643. Dihydrodiol_DH_HcaB.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12."
    Diaz E., Ferrandez A., Garcia J.L.
    J. Bacteriol. 180:2915-2923(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CATABOLISM OF PHENYLPROPIONIC AND CINNAMIC ACIDS, CATALYTIC ACTIVITY.
    Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.

Entry informationi

Entry nameiHCAB_ECOLI
AccessioniPrimary (citable) accession number: P0CI31
Secondary accession number(s): P76995, P77646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: January 7, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.