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P0CI31

- HCAB_ECOLI

UniProt

P0CI31 - HCAB_ECOLI

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Protein

3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase

Gene
hcaB, phdD, yfhX, b2541, JW2525
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively By similarity.1 Publication

Catalytic activityi

3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+ = 3-(2,3-dihydroxyphenyl)propanoate + NADH.1 Publication
(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+ = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NADH.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431Substrate By similarity
Active sitei156 – 1561Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3425NAD By similarityAdd
BLAST

GO - Molecular functioni

  1. 2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:PHENPRODIOLDEHYDROG-MONOMER.
MetaCyc:PHENPRODIOLDEHYDROG-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (EC:1.3.1.87)
Alternative name(s):
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase
CI-dihydrodiol dehydrogenase
Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
PP-dihydrodiol dehydrogenase
Gene namesi
Name:hcaB
Synonyms:phdD, yfhX
Ordered Locus Names:b2541, JW2525
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13459. hcaB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2702703-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenaseUniRule annotationPRO_0000054704Add
BLAST

Proteomic databases

PRIDEiP0CI31.

Interactioni

Protein-protein interaction databases

DIPiDIP-9863N.
IntActiP0CI31. 7 interactions.
MINTiMINT-1239788.

Structurei

3D structure databases

ProteinModelPortaliP0CI31.
SMRiP0CI31. Positions 4-270.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK05711.
OMAiTASKHAC.
OrthoDBiEOG625JVT.
PhylomeDBiP0CI31.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01647. HcaB.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR023643. Dihydrodiol_DH_HcaB.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CI31-1 [UniParc]FASTAAdd to Basket

« Hide

MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF    50
GEHILAVEGN VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT 100
PAETLETGFH ELFNVNVLGY LLGAKACAPA LIASEGSMIF TLSNAAWYPG 150
GGGPLYTASK HAATGLIRQL AYELAPKVRV NGVGPCGMAS DLRGPQALGQ 200
SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLTSRR NNRALSGVMI 250
NADAGLAIRG IRHVAAGLDL 270
Length:270
Mass (Da):28,500
Last modified:November 30, 2010 - v1
Checksum:i607D316E7442D3E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75594.1.
AP009048 Genomic DNA. Translation: BAA16444.1.
PIRiD65031.
RefSeqiNP_417036.1. NC_000913.3.
YP_490769.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75594; AAC75594; b2541.
BAA16444; BAA16444; BAA16444.
GeneIDi12931610.
945346.
KEGGiecj:Y75_p2494.
eco:b2541.
PATRICi32120479. VBIEscCol129921_2642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75594.1 .
AP009048 Genomic DNA. Translation: BAA16444.1 .
PIRi D65031.
RefSeqi NP_417036.1. NC_000913.3.
YP_490769.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0CI31.
SMRi P0CI31. Positions 4-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9863N.
IntActi P0CI31. 7 interactions.
MINTi MINT-1239788.

Proteomic databases

PRIDEi P0CI31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75594 ; AAC75594 ; b2541 .
BAA16444 ; BAA16444 ; BAA16444 .
GeneIDi 12931610.
945346.
KEGGi ecj:Y75_p2494.
eco:b2541.
PATRICi 32120479. VBIEscCol129921_2642.

Organism-specific databases

EchoBASEi EB3232.
EcoGenei EG13459. hcaB.

Phylogenomic databases

KOi K05711.
OMAi TASKHAC.
OrthoDBi EOG625JVT.
PhylomeDBi P0CI31.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci EcoCyc:PHENPRODIOLDEHYDROG-MONOMER.
MetaCyc:PHENPRODIOLDEHYDROG-MONOMER.

Miscellaneous databases

PROi P0CI31.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_01647. HcaB.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR023643. Dihydrodiol_DH_HcaB.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12."
    Diaz E., Ferrandez A., Garcia J.L.
    J. Bacteriol. 180:2915-2923(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CATABOLISM OF PHENYLPROPIONIC AND CINNAMIC ACIDS, CATALYTIC ACTIVITY.
    Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.

Entry informationi

Entry nameiHCAB_ECOLI
AccessioniPrimary (citable) accession number: P0CI31
Secondary accession number(s): P76995, P77646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: May 14, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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