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Protein

Phospholipase A1

Gene
N/A
Organism
Vespula squamosa (Southern yellow jacket) (Wasp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of phosphatidylcholine with phospholipase A1 (EC 3.1.1.32) activity. Has weak hemolytic activity (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei134 – 1341NucleophileBy similarity
Active sitei162 – 1621Charge relay systemPROSITE-ProRule annotation
Active sitei227 – 2271Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cytolysis, Hemolysis, Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A1 (EC:3.1.1.32)
Alternative name(s):
Allergen: Ves s 1
OrganismiVespula squamosa (Southern yellow jacket) (Wasp)
Taxonomic identifieri30214 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3518. Ves s 1.0101.
665. Ves s 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Phospholipase A1PRO_0000401921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 ↔ 242By similarity
Disulfide bondi87 ↔ 225By similarity
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence analysis
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence analysis
Disulfide bondi173 ↔ 216By similarity
Disulfide bondi178 ↔ 259By similarity
Disulfide bondi237 ↔ 243By similarity
Disulfide bondi266 ↔ 291By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP0CH86.

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002334. Allerg_PlipaseA1.
IPR013818. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PRINTSiPR00825. DOLALLERGEN.
PR00821. TAGLIPASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CH86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GSKCPFSDDT VAMVIVTREN RNRDFYTLQT LRNHDEFKKK AITRPVVFIT
60 70 80 90 100
HGFTSSATVE SFVDLQTAIL EXXXXKVTVS DWRVAACNRT TGLLYYVTAV
110 120 130 140 150
SNTRLVGRYI ATVTKKLVTD YNVSMADIRL IGHSLGAHVS GFAGKEVQKL
160 170 180 190 200
KLEKYSEIIG LDPAGPSFES NDCAERLCKT DAHYVQIIHT SKKFGIEKSI
210 220 230 240 250
GHVDFYVNQG NNQPGCGIIP LKDVCSHSRA ITYMTECIKR ECCLIGIPQS
260 270 280 290
KSSKSISSCT RQECVCVGLK AKSYPNTGSF YVPVESTAPF CNNKGKII
Length:298
Mass (Da):32,934
Last modified:November 30, 2010 - v1
Checksum:iAAC3822E61263EEF
GO

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3518. Ves s 1.0101.
665. Ves s 1.

Proteomic databases

PRIDEiP0CH86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002334. Allerg_PlipaseA1.
IPR013818. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PRINTSiPR00825. DOLALLERGEN.
PR00821. TAGLIPASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sol i 1, the phospholipase allergen of imported fire ant venom."
    Hoffman D.R., Sakell R.H., Schmidt M.
    J. Allergy Clin. Immunol. 115:611-616(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Venom.

Entry informationi

Entry nameiPA1_VESSQ
AccessioniPrimary (citable) accession number: P0CH86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: January 7, 2015
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.