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Protein

Cyanamide hydratase DDI2

Gene

DDI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cyanamide hydratase involved in the detoxification and/or utilization of cyanamide, a toxic nitrile compound distributed widely in the environment.1 Publication

Catalytic activityi

Urea = cyanamide + H2O.1 Publication

Cofactori

Zn2+By similarity

Kineticsi

  1. KM=17.3 mM for cyanamide.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30406-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyanamide hydratase DDI21 Publication (EC:4.2.1.691 Publication)
    Short name:
    CAH1 Publication
    Alternative name(s):
    DNA damage-inducible protein 21 Publication
    Gene namesi
    Name:DDI21 Publication
    Ordered Locus Names:YFL061WImported
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311 Componenti: Chromosome VI

    Organism-specific databases

    SGDiS000001833. DDI2.

    Pathology & Biotechi

    Disruption phenotypei

    The double deletion of DDI2 and DDI3 compromizes cellular resistance to cyanamide by impairing its metabolization.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225Cyanamide hydratase DDI2PRO_0000202670Add
    BLAST

    Expressioni

    Inductioni

    Expression is induced by DNA-damaging agents such as methyl methanesulfonate (MMS) or dimethyl sulfate (DMS) (PubMed:18485869, PubMed:25847245). Massively induced by cyanamide (PubMed:25847245).2 Publications

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    BioGridi35506. 2 interactions.
    STRINGi4932.YNL335W.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CH63.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini52 – 155104HDSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cyanamide dehydrase family.Curated
    Contains 1 HD domain.Sequence Analysis

    Phylogenomic databases

    InParanoidiP0CH63.
    OrthoDBiEOG7CRV1M.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    InterProiIPR017771. Cyanamide_hydratase_HD.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03401. cyanamide_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0CH63-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSQYGFVRVP REVEKAIPVV NAPRPRAVVP PPNSETARLV REYAAKELTA
    60 70 80 90 100
    PVLNHSLRVF QYSVAIIRDQ FPAWDLDQEV LYVTCLLHDI ATTDKNMRAT
    110 120 130 140 150
    KMSFEYYGGI LSRELVFNAT GGNQDYADAV TEAIIRHQDL TGTGYITTLG
    160 170 180 190 200
    LILQIATTLD NVGSNTDLIH IDTVSAINEQ FPRLHWLSCF ATVVDTENSR
    210 220
    KPWGHTSSLG DDFSKKVICN TFGYN
    Length:225
    Mass (Da):25,152
    Last modified:October 5, 2010 - v1
    Checksum:iF4890BF675E652B2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50617 Genomic DNA. Translation: BAA09180.1.
    AY692638 Genomic DNA. Translation: AAT92657.1.
    BK006940 Genomic DNA. Translation: DAA12379.1.
    PIRiS56194.
    RefSeqiNP_014064.1. NM_001183173.1.
    NP_116594.1. NM_001179906.1.

    Genome annotation databases

    EnsemblFungiiYFL061W; YFL061W; YFL061W.
    YNL335W; YNL335W; YNL335W.
    GeneIDi850483.
    855381.
    KEGGisce:YFL061W.
    sce:YNL335W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50617 Genomic DNA. Translation: BAA09180.1.
    AY692638 Genomic DNA. Translation: AAT92657.1.
    BK006940 Genomic DNA. Translation: DAA12379.1.
    PIRiS56194.
    RefSeqiNP_014064.1. NM_001183173.1.
    NP_116594.1. NM_001179906.1.

    3D structure databases

    ProteinModelPortaliP0CH63.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35506. 2 interactions.
    STRINGi4932.YNL335W.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYFL061W; YFL061W; YFL061W.
    YNL335W; YNL335W; YNL335W.
    GeneIDi850483.
    855381.
    KEGGisce:YFL061W.
    sce:YNL335W.

    Organism-specific databases

    SGDiS000001833. DDI2.

    Phylogenomic databases

    InParanoidiP0CH63.
    OrthoDBiEOG7CRV1M.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30406-MONOMER.

    Miscellaneous databases

    NextBioi966143.
    PROiP0CH63.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    InterProiIPR017771. Cyanamide_hydratase_HD.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03401. cyanamide_fam. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp."
      Fu Y., Zhu Y., Zhang K., Yeung M., Durocher D., Xiao W.
      Cell 133:601-611(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    5. "Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide."
      Li J., Biss M., Fu Y., Xu X., Moore S.A., Xiao W.
      J. Biol. Chem. 0:0-0(2015) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiDDI2_YEAST
    AccessioniPrimary (citable) accession number: P0CH63
    Secondary accession number(s): D6VTG9, P43543
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: June 24, 2015
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    DDI2 and DDI3 are duplicated genes located on different chromosomes, with identical ORF sequences and only one nucleotide difference in their promoter (up to 1 kb) regions.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.