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P0CH29 (NPRM_BACME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bacillolysin

EC=3.4.24.28
Alternative name(s):
Neutral protease
Gene names
Name:nprM
OrganismBacillus megaterium
Taxonomic identifier1404 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

Biophysicochemical properties

Temperature dependence:

Thermostable.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 245221Activation peptide Potential
PRO_0000028610
Chain246 – 562317Bacillolysin
PRO_0000028611

Sites

Active site3891 By similarity
Active site4771Proton donor By similarity
Metal binding3031Calcium 1 By similarity
Metal binding3051Calcium 1 By similarity
Metal binding3841Calcium 2 By similarity
Metal binding3881Zinc; catalytic By similarity
Metal binding3921Zinc; catalytic By similarity
Metal binding4121Zinc; catalytic By similarity
Metal binding4231Calcium 2 By similarity
Metal binding4231Calcium 3 By similarity
Metal binding4291Calcium 3; via carbonyl oxygen By similarity
Metal binding4311Calcium 2 By similarity
Metal binding4311Calcium 3 By similarity
Metal binding4331Calcium 2; via carbonyl oxygen By similarity
Metal binding4361Calcium 2 By similarity
Metal binding4361Calcium 3 By similarity
Metal binding4391Calcium 4; via carbonyl oxygen By similarity
Metal binding4401Calcium 4 By similarity
Metal binding4461Calcium 4 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0CH29 [UniParc].

Last modified August 10, 2010. Version 1.
Checksum: 17203441C7F6AAB7

FASTA56260,949
        10         20         30         40         50         60 
MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI SGKLSGPSSQ 

        70         80         90        100        110        120 
KAQDVVFHYM NTNKDKYKLG NESAQNSFKV TEVVKDPVEQ ATVVRLQQVY NNIPVWGSTQ 

       130        140        150        160        170        180 
LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL 

       190        200        210        220        230        240 
YVYQNASDTT YAYVVNLNFL SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK 

       250        260        270        280        290        300 
PAAKPVTGTN TIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL 

       310        320        330        340        350        360 
WADTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGRPLKSTV HYSSGYNNAF 

       370        380        390        400        410        420 
WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THALTERSSN LIYQYESGAL NEAISDIFGT 

       430        440        450        460        470        480 
LVEYYDNRNP DWEIGEDIYT PGTSGDALRS MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS 

       490        500        510        520        530        540 
GIINKAAYLL ANGGTHYGVT VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD 

       550        560 
LYGSGSQEVI SVGKSFDAVG VQ 

« Hide

References

[1]"Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581."
Kuhn S., Fortnagel P.
J. Gen. Microbiol. 139:39-47(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61380 Genomic DNA. Translation: CAA43654.1.
PIRA47710.

3D structure databases

ProteinModelPortalP0CH29.
SMRP0CH29. Positions 246-561.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.170.10. 1 hit.
InterProIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNPRM_BACME
AccessionPrimary (citable) accession number: P0CH29
Secondary accession number(s): Q00891
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: May 14, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries