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Protein

Bacillolysin

Gene

nprM

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.1 Publication

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+1 PublicationNote: Binds 4 Ca2+ ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

pH dependencei

Optimum pH is between 6.4 and 7.2.1 Publication

Temperature dependencei

Optimum temperature is 58 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi303 – 3031Calcium 1By similarity
Metal bindingi305 – 3051Calcium 1By similarity
Metal bindingi384 – 3841Calcium 2By similarity
Metal bindingi388 – 3881Zinc; catalyticBy similarity
Active sitei389 – 3891By similarity
Metal bindingi392 – 3921Zinc; catalyticBy similarity
Metal bindingi412 – 4121Zinc; catalyticBy similarity
Metal bindingi423 – 4231Calcium 2By similarity
Metal bindingi423 – 4231Calcium 3By similarity
Metal bindingi429 – 4291Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi431 – 4311Calcium 2By similarity
Metal bindingi431 – 4311Calcium 3By similarity
Metal bindingi433 – 4331Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi436 – 4361Calcium 2By similarity
Metal bindingi436 – 4361Calcium 3By similarity
Metal bindingi439 – 4391Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi440 – 4401Calcium 4By similarity
Metal bindingi446 – 4461Calcium 4By similarity
Active sitei477 – 4771Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Calcium-dependent exoproteinase1 Publication
Neutral protease1 Publication
Gene namesi
Name:nprM1 Publication
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Propeptidei25 – 245221Activation peptide1 PublicationPRO_0000028610Add
BLAST
Chaini246 – 562317BacillolysinPRO_0000028611Add
BLAST

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

STRINGi545693.BMQ_2326.

Structurei

3D structure databases

ProteinModelPortaliP0CH29.
SMRiP0CH29. Positions 246-561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CH29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI
60 70 80 90 100
SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NESAQNSFKV TEVVKDPVEQ
110 120 130 140 150
ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ
160 170 180 190 200
KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNASDTT YAYVVNLNFL
210 220 230 240 250
SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN
260 270 280 290 300
TIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL
310 320 330 340 350
WADTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGRPLKSTV
360 370 380 390 400
HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THALTERSSN
410 420 430 440 450
LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS
460 470 480 490 500
MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT
510 520 530 540 550
VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI
560
SVGKSFDAVG VQ
Length:562
Mass (Da):60,949
Last modified:August 10, 2010 - v1
Checksum:i17203441C7F6AAB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61380 Genomic DNA. Translation: CAA43654.1.
PIRiA47710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61380 Genomic DNA. Translation: CAA43654.1.
PIRiA47710.

3D structure databases

ProteinModelPortaliP0CH29.
SMRiP0CH29. Positions 246-561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi545693.BMQ_2326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581."
    Kuhn S., Fortnagel P.
    J. Gen. Microbiol. 139:39-47(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBCELLULAR LOCATION.
    Strain: ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512.

Entry informationi

Entry nameiNPRM_BACME
AccessioniPrimary (citable) accession number: P0CH29
Secondary accession number(s): Q00891
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: July 22, 2015
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.