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P0CH29

- NPRM_BACME

UniProt

P0CH29 - NPRM_BACME

Protein

Bacillolysin

Gene

nprM

Organism
Bacillus megaterium
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 18 (01 Oct 2014)
      Sequence version 1 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Extracellular zinc metalloprotease.

    Catalytic activityi

    Similar, but not identical, to that of thermolysin.

    Cofactori

    Binds 4 calcium ions per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Temperature dependencei

    Thermostable.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi303 – 3031Calcium 1By similarity
    Metal bindingi305 – 3051Calcium 1By similarity
    Metal bindingi384 – 3841Calcium 2By similarity
    Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
    Active sitei389 – 3891PROSITE-ProRule annotation
    Metal bindingi392 – 3921Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi412 – 4121Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi423 – 4231Calcium 2By similarity
    Metal bindingi423 – 4231Calcium 3By similarity
    Metal bindingi429 – 4291Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi431 – 4311Calcium 2By similarity
    Metal bindingi431 – 4311Calcium 3By similarity
    Metal bindingi433 – 4331Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi436 – 4361Calcium 2By similarity
    Metal bindingi436 – 4361Calcium 3By similarity
    Metal bindingi439 – 4391Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi440 – 4401Calcium 4By similarity
    Metal bindingi446 – 4461Calcium 4By similarity
    Active sitei477 – 4771Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacillolysin (EC:3.4.24.28)
    Alternative name(s):
    Neutral protease
    Gene namesi
    Name:nprM
    OrganismiBacillus megaterium
    Taxonomic identifieri1404 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 245221Activation peptideSequence AnalysisPRO_0000028610Add
    BLAST
    Chaini246 – 562317BacillolysinPRO_0000028611Add
    BLAST

    Keywords - PTMi

    Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP0CH29.
    SMRiP0CH29. Positions 246-561.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CH29-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI    50
    SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NESAQNSFKV TEVVKDPVEQ 100
    ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ 150
    KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNASDTT YAYVVNLNFL 200
    SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN 250
    TIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL 300
    WADTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGRPLKSTV 350
    HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THALTERSSN 400
    LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS 450
    MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT 500
    VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI 550
    SVGKSFDAVG VQ 562
    Length:562
    Mass (Da):60,949
    Last modified:August 10, 2010 - v1
    Checksum:i17203441C7F6AAB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61380 Genomic DNA. Translation: CAA43654.1.
    PIRiA47710.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61380 Genomic DNA. Translation: CAA43654.1 .
    PIRi A47710.

    3D structure databases

    ProteinModelPortali P0CH29.
    SMRi P0CH29. Positions 246-561.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581."
      Kuhn S., Fortnagel P.
      J. Gen. Microbiol. 139:39-47(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512.

    Entry informationi

    Entry nameiNPRM_BACME
    AccessioniPrimary (citable) accession number: P0CH29
    Secondary accession number(s): Q00891
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3