P0CH12 (KA125_LYCMC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 12.5 Alternative name(s): Toxin alpha-KTx10 Short name=LmKTx10
Organism	Lychas mucronatus (Chinese swimming scorpion)
Taxonomic identifier	172552 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Lychas

Protein attributes

Sequence length	60 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This recombinant toxin inhibits the mammalian voltage-gated potassium channels Kv1.3/KCNA3 in vitro with an IC50 of 28 nM. Kv1.1/KCNA1 and Kv1.2/KCNA2 potassium channels are also weakly inhibited (IC50= 1.73 µM and IC50=12.63 µM).
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 12 subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 60	38	Potassium channel toxin alpha-KTx 12.5		PRO_0000396529
Regions
	Compositional bias	31 – 35	5	Poly-Ser
Sites
	Site	50	1	Basic residue of the functional dyad By similarity
	Site	59	1	Aromatic residue of the functional dyad By similarity
Amino acid modifications
	Disulfide bond	30 ↔ 51		By similarity
	Disulfide bond	36 ↔ 56		By similarity
	Disulfide bond	40 ↔ 58		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0CH12 [UniParc]. Last modified August 10, 2010. Version 1. Checksum: AFD82216067F291B	FASTA	60	6,720
	10 20 30 40 50 60 MNKLPILIFM LLVCSMFISS DCQKHTDIKC SSSSSCYEPC RGVTGRAHGK CMNGRCTCYY

References

[1]	"Molecular cloning and functional identification of a new K(+) channel blocker, LmKTx10, from the scorpion Lychas mucronatus." Liu J., Ma Y., Yin S., Zhao R., Fan S., Hu Y., Wu Y., Cao Z., Li W. Peptides 30:675-680(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
[2]	"Comparative venom gland transcriptome analysis of the scorpion Lychas mucronatus reveals intraspecific toxic gene diversity and new venomous components." Zhao R., Ma Y., He Y., Di Z., Wu Y.-L., Cao Z.-J., Li W.-X. BMC Genomics 11:452-452(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hainan. Tissue: Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU163856 mRNA. Translation: ABY26665.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PRINTS	PR00286. CHARYBDTOXIN.
ProDom	PD003586. Scorpion_toxinS. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KA125_LYCMC

Accession

Primary (citable) accession number: P0CH12
Secondary accession number(s): D9U2A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	August 10, 2010
Last modified:	May 1, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program