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Protein

Ribonucleoside-diphosphate reductase subunit alpha 2

Gene

nrdE2

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei169SubstrateBy similarity1
Sitei186Important for hydrogen atom transferBy similarity1
Sitei193Allosteric effector bindingBy similarity1
Binding sitei214Substrate; via amide nitrogenBy similarity1
Sitei223Allosteric effector bindingBy similarity1
Active sitei394Proton acceptorBy similarity1
Active sitei396Cysteine radical intermediateBy similarity1
Active sitei398Proton acceptorBy similarity1
Sitei423Important for hydrogen atom transferBy similarity1
Sitei700Important for electron transferBy similarity1
Sitei701Important for electron transferBy similarity1
Sitei717Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei720Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha 2 (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R1 subunit 2
Gene namesi
Name:nrdE2
Ordered Locus Names:MSMEG_2299, MSMEI_2241
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003961041 – 722Ribonucleoside-diphosphate reductase subunit alpha 2Add BLAST722

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi186 ↔ 423Redox-activeBy similarity
Cross-linki310Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Disulfide bond, Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_2299.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 186Substrate bindingBy similarity2
Regioni394 – 398Substrate bindingBy similarity5
Regioni596 – 600Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
HOGENOMiHOG000246165.
KOiK00525.
OrthoDBiPOG091H03NG.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CH00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPTVTAAEP VTTTGHVLPG EADYHALNAM LNLYDADGKI QFEKDREAAK
60 70 80 90 100
QYFLQHVNQN TVFFHSQDEK LDYLIENEYY EREVLDQYSR DFIKSLLDRA
110 120 130 140 150
YAKKFRFPTF LGAFKYYTSY TLKTFDGKRY LERFEDRVVM VALTLAAGDT
160 170 180 190 200
ELAEKLVDEI IDGRFQPATP TFLNSGKKQR GEPVSCFLLR IEDNMESIGR
210 220 230 240 250
SINSALQLSK RGGGVALLLS NIREHGAPIK NIENQSSGVI PIMKLLEDSF
260 270 280 290 300
SYANQLGARQ GAGAVYLHAH HPDIYRFLDT KRENADEKIR IKTLSLGVVI
310 320 330 340 350
PDITFELAKK NEDMYLFSPY DVERVYGVPF ADVSVTEKYY EMVDDARIRK
360 370 380 390 400
TKINAREFFQ TLAELQFESG YPYIMFEDTV NRSNPIAGKI THSNLCSEIL
410 420 430 440 450
QVSTPSEFND DLSYAKVGKD ISCNLGSLNI AKAMDSPDFA QTIEVAIRAL
460 470 480 490 500
TAVSDQTHIT SVPSIEQGNN DSHAIGLGQM NLHGYLARER IYYGSEEGID
510 520 530 540 550
FTNIYFYTVL FHALRASNKI AIERGTHFKG FEKSKYASGE FFDKYTDQVW
560 570 580 590 600
EPKTDKVRRL FADADIHIPT QDDWKQLKES VQKHGIYNQN LQAVPPTGSI
610 620 630 640 650
SYINHSTSSI HPVASKIEIR KEGKIGRVYY PAPYMTNDNL DYYQDAYEIG
660 670 680 690 700
YEKIIDTYAA ATQHVDQGLS LTLFFKDTAT TRDVNKAQIY AWRKGIKTLY
710 720
YIRLRQMALE GTEVEGCVSC ML
Length:722
Mass (Da):82,106
Last modified:August 10, 2010 - v1
Checksum:i9D24EFB724737F9A
GO

Sequence cautioni

The sequence ABK70217 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70217.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP38711.1.
RefSeqiWP_014876938.1. NZ_CP009494.1.
YP_885419.1. NC_008596.1.
YP_886648.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70217; ABK70217; MSMEG_2299.
AFP38711; AFP38711; MSMEI_2241.
GeneIDi4531391.
4535428.
KEGGimsb:LJ00_05050.
msb:LJ00_11435.
msg:MSMEI_2241.
msm:MSMEG_1019.
msm:MSMEG_2299.
PATRICi18074490. VBIMycSme59918_1006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70217.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP38711.1.
RefSeqiWP_014876938.1. NZ_CP009494.1.
YP_885419.1. NC_008596.1.
YP_886648.1. NC_008596.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70217; ABK70217; MSMEG_2299.
AFP38711; AFP38711; MSMEI_2241.
GeneIDi4531391.
4535428.
KEGGimsb:LJ00_05050.
msb:LJ00_11435.
msg:MSMEI_2241.
msm:MSMEG_1019.
msm:MSMEG_2299.
PATRICi18074490. VBIMycSme59918_1006.

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
HOGENOMiHOG000246165.
KOiK00525.
OrthoDBiPOG091H03NG.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1B_MYCS2
AccessioniPrimary (citable) accession number: P0CH00
Secondary accession number(s): A0QR81, I7F7C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: November 30, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Pupylation of this protein has been demonstrated, although it is unknown if the protein concerned is the product of this gene, of the identical gene MSMEG_1019 (AC P0CG99), or of both genes.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.