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Protein

Polyubiquitin

Gene

Ubi-p63E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity

Miscellaneous

In Drosophila ubiquitin is encoded by 3 different genes. RpL40 and RpS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. Ubi-p63E gene codes for a polyubiquitin precursor with 10 exact head to tail repeats.
For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Activating enzyme1
Sitei68Essential for function1
Binding sitei72Activating enzyme1

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: FlyBase
  • larval midgut cell programmed cell death Source: FlyBase
  • protein ubiquitination Source: FlyBase

Enzyme and pathway databases

ReactomeiR-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209447 Activation of the IkappaB kinase complex, KEY:IRD5 dimer:KEY
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-216167 Nuclear CI is degraded
R-DME-432395 Degradation of TIM
R-DME-432524 Degradation of PER
R-DME-538864 Degradation of CRY

Names & Taxonomyi

Protein namesi
Recommended name:
Polyubiquitin
Cleaved into the following chain:
Gene namesi
Name:Ubi-p63E
Synonyms:Ubi-m
ORF Names:CG11624
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0003943 Ubi-p63E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001148131 – 76UbiquitinAdd BLAST76
ChainiPRO_000039626077 – 152UbiquitinAdd BLAST76
ChainiPRO_0000396261153 – 228UbiquitinAdd BLAST76
ChainiPRO_0000396262229 – 304UbiquitinAdd BLAST76
ChainiPRO_0000396263305 – 380UbiquitinAdd BLAST76
ChainiPRO_0000396264381 – 456UbiquitinAdd BLAST76
ChainiPRO_0000396265457 – 532UbiquitinAdd BLAST76
ChainiPRO_0000396266533 – 608UbiquitinAdd BLAST76
ChainiPRO_0000396267609 – 684UbiquitinAdd BLAST76
ChainiPRO_0000396268685 – 760UbiquitinAdd BLAST76
PropeptideiPRO_0000396269761 – 7633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP0CG69
PRIDEiP0CG69

Expressioni

Gene expression databases

BgeeiFBgn0003943
ExpressionAtlasiP0CG69 baseline and differential
GenevisibleiP0CG69 DM

Interactioni

Protein-protein interaction databases

BioGridi63944, 28 interactors
STRINGi7227.FBpp0073035

Structurei

3D structure databases

ProteinModelPortaliP0CG69
SMRiP0CG69
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST76
Domaini77 – 152Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST76
Domaini153 – 228Ubiquitin-like 3PROSITE-ProRule annotationAdd BLAST76
Domaini229 – 304Ubiquitin-like 4PROSITE-ProRule annotationAdd BLAST76
Domaini305 – 380Ubiquitin-like 5PROSITE-ProRule annotationAdd BLAST76
Domaini381 – 456Ubiquitin-like 6PROSITE-ProRule annotationAdd BLAST76
Domaini457 – 532Ubiquitin-like 7PROSITE-ProRule annotationAdd BLAST76
Domaini533 – 608Ubiquitin-like 8PROSITE-ProRule annotationAdd BLAST76
Domaini609 – 684Ubiquitin-like 9PROSITE-ProRule annotationAdd BLAST76
Domaini685 – 760Ubiquitin-like 10PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00810000125435
InParanoidiP0CG69
KOiK08770
OMAiHLTMQIF
OrthoDBiEOG091G178I
PhylomeDBiP0CG69

Family and domain databases

InterProiView protein in InterPro
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 10 hits
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 10 hits
SUPFAMiSSF54236 SSF54236, 10 hits
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 10 hits
PS50053 UBIQUITIN_2, 10 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CG69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE
110 120 130 140 150
NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR
160 170 180 190 200
GGMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK
210 220 230 240 250
QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT
260 270 280 290 300
IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR
310 320 330 340 350
LRGGMQIFVK TLTGKTITLE VEPSDTIENV KAKIQDKEGI PPDQQRLIFA
360 370 380 390 400
GKQLEDGRTL SDYNIQKEST LHLVLRLRGG MQIFVKTLTG KTITLEVEPS
410 420 430 440 450
DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV
460 470 480 490 500
LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI
510 520 530 540 550
FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GGMQIFVKTL TGKTITLEVE
560 570 580 590 600
PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLEDGRTLSD YNIQKESTLH
610 620 630 640 650
LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR
660 670 680 690 700
LIFAGKQLED GRTLSDYNIQ KESTLHLVLR LRGGMQIFVK TLTGKTITLE
710 720 730 740 750
VEPSDTIENV KAKIQDKEGI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST
760
LHLVLRLRGG IQA
Length:763
Mass (Da):85,799
Last modified:August 10, 2010 - v1
Checksum:i5693123AE7B186A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF47806.3
M33013 Genomic DNA Translation: AAA28999.1
M33015 Genomic DNA Translation: AAA29001.1
M33122 Genomic DNA Translation: AAA29007.1
M22428 Genomic DNA Translation: AAA28997.1
PIRiA26087
RefSeqiNP_001261383.1, NM_001274454.1
NP_523909.2, NM_079185.4
NP_728908.1, NM_168043.3
NP_995994.1, NM_206272.1
UniGeneiDm.2641

Genome annotation databases

EnsemblMetazoaiFBtr0073177; FBpp0073034; FBgn0003943
FBtr0073178; FBpp0073035; FBgn0003943
FBtr0073179; FBpp0089269; FBgn0003943
FBtr0333142; FBpp0305348; FBgn0003943
GeneIDi38456
KEGGidme:Dmel_CG11624

Similar proteinsi

Entry informationi

Entry nameiUBIQP_DROME
AccessioniPrimary (citable) accession number: P0CG69
Secondary accession number(s): P68198
, Q0E8I1, Q9VKW6, Q9VQX7, Q9VZL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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