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Protein

Polyubiquitin

Gene

Ubi-p63E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity

Miscellaneous

In Drosophila ubiquitin is encoded by 3 different genes. RpL40 and RpS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. Ubi-p63E gene codes for a polyubiquitin precursor with 10 exact head to tail repeats.
For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Activating enzyme1
Sitei68Essential for function1
Binding sitei72Activating enzyme1

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: FlyBase
  • larval midgut cell programmed cell death Source: FlyBase
  • Notch signaling pathway Source: Reactome
  • phagocytosis Source: FlyBase
  • protein ubiquitination Source: FlyBase

Enzyme and pathway databases

ReactomeiR-DME-110312. Translesion synthesis by REV1.
R-DME-110320. Translesion Synthesis by POLH.
R-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1253288. Downregulation of ERBB4 signaling.
R-DME-1295596. Spry regulation of FGF signaling.
R-DME-1358803. Downregulation of ERBB2:ERBB3 signaling.
R-DME-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-179409. APC-Cdc20 mediated degradation of Nek2A.
R-DME-182971. EGFR downregulation.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-201681. TCF dependent signaling in response to WNT.
R-DME-209560. NF-kB is activated and signals survival.
R-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2173788. Downregulation of TGF-beta receptor signaling.
R-DME-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-DME-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-DME-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-DME-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.
R-DME-3769402. Deactivation of the beta-catenin transactivating complex.
R-DME-446652. Interleukin-1 family signaling.
R-DME-450302. activated TAK1 mediates p38 MAPK activation.
R-DME-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5205685. Pink/Parkin Mediated Mitophagy.
R-DME-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5654726. Negative regulation of FGFR1 signaling.
R-DME-5654727. Negative regulation of FGFR2 signaling.
R-DME-5654732. Negative regulation of FGFR3 signaling.
R-DME-5654733. Negative regulation of FGFR4 signaling.
R-DME-5655862. Translesion synthesis by POLK.
R-DME-5656121. Translesion synthesis by POLI.
R-DME-5656169. Termination of translesion DNA synthesis.
R-DME-5675221. Negative regulation of MAPK pathway.
R-DME-5675482. Regulation of necroptotic cell death.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689877. Josephin domain DUBs.
R-DME-5689880. Ub-specific processing proteases.
R-DME-5689896. Ovarian tumor domain proteases.
R-DME-5689901. Metalloprotease DUBs.
R-DME-5696394. DNA Damage Recognition in GG-NER.
R-DME-5696395. Formation of Incision Complex in GG-NER.
R-DME-6781823. Formation of TC-NER Pre-Incision Complex.
R-DME-6782135. Dual incision in TC-NER.
R-DME-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69231. Cyclin D associated events in G1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8849469. PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-DME-8856828. Clathrin-mediated endocytosis.
R-DME-8863795. Downregulation of ERBB2 signaling.
R-DME-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-DME-8866654. E3 ubiquitin ligases ubiquitinate target proteins.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-8948747. Regulation of PTEN localization.
R-DME-8948751. Regulation of PTEN stability and activity.
R-DME-901032. ER Quality Control Compartment (ERQC).
R-DME-912631. Regulation of signaling by CBL.
R-DME-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
R-DME-917937. Iron uptake and transport.
R-DME-937039. IRAK1 recruits IKK complex.
R-DME-937042. IRAK2 mediated activation of TAK1 complex.
R-DME-937072. TRAF6 mediated induction of TAK1 complex.
R-DME-975110. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
R-DME-975144. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
R-DME-975163. IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyubiquitin
Cleaved into the following chain:
Gene namesi
Name:Ubi-p63E
Synonyms:Ubi-m
ORF Names:CG11624
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0003943. Ubi-p63E.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001148131 – 76UbiquitinAdd BLAST76
ChainiPRO_000039626077 – 152UbiquitinAdd BLAST76
ChainiPRO_0000396261153 – 228UbiquitinAdd BLAST76
ChainiPRO_0000396262229 – 304UbiquitinAdd BLAST76
ChainiPRO_0000396263305 – 380UbiquitinAdd BLAST76
ChainiPRO_0000396264381 – 456UbiquitinAdd BLAST76
ChainiPRO_0000396265457 – 532UbiquitinAdd BLAST76
ChainiPRO_0000396266533 – 608UbiquitinAdd BLAST76
ChainiPRO_0000396267609 – 684UbiquitinAdd BLAST76
ChainiPRO_0000396268685 – 760UbiquitinAdd BLAST76
PropeptideiPRO_0000396269761 – 7633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP0CG69.

Expressioni

Gene expression databases

BgeeiFBgn0003943.
ExpressionAtlasiP0CG69. differential.
GenevisibleiP0CG69. DM.

Interactioni

Protein-protein interaction databases

BioGridi63944. 28 interactors.
STRINGi7227.FBpp0073035.

Structurei

3D structure databases

ProteinModelPortaliP0CG69.
SMRiP0CG69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST76
Domaini77 – 152Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST76
Domaini153 – 228Ubiquitin-like 3PROSITE-ProRule annotationAdd BLAST76
Domaini229 – 304Ubiquitin-like 4PROSITE-ProRule annotationAdd BLAST76
Domaini305 – 380Ubiquitin-like 5PROSITE-ProRule annotationAdd BLAST76
Domaini381 – 456Ubiquitin-like 6PROSITE-ProRule annotationAdd BLAST76
Domaini457 – 532Ubiquitin-like 7PROSITE-ProRule annotationAdd BLAST76
Domaini533 – 608Ubiquitin-like 8PROSITE-ProRule annotationAdd BLAST76
Domaini609 – 684Ubiquitin-like 9PROSITE-ProRule annotationAdd BLAST76
Domaini685 – 760Ubiquitin-like 10PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00810000125435.
InParanoidiP0CG69.
KOiK08770.
OMAiHLTMQIF.
OrthoDBiEOG091G178I.
PhylomeDBiP0CG69.

Family and domain databases

InterProiView protein in InterPro
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 10 hits.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM00213. UBQ. 10 hits.
SUPFAMiSSF54236. SSF54236. 10 hits.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 10 hits.
PS50053. UBIQUITIN_2. 10 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CG69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE
110 120 130 140 150
NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR
160 170 180 190 200
GGMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK
210 220 230 240 250
QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT
260 270 280 290 300
IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR
310 320 330 340 350
LRGGMQIFVK TLTGKTITLE VEPSDTIENV KAKIQDKEGI PPDQQRLIFA
360 370 380 390 400
GKQLEDGRTL SDYNIQKEST LHLVLRLRGG MQIFVKTLTG KTITLEVEPS
410 420 430 440 450
DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV
460 470 480 490 500
LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI
510 520 530 540 550
FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GGMQIFVKTL TGKTITLEVE
560 570 580 590 600
PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLEDGRTLSD YNIQKESTLH
610 620 630 640 650
LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR
660 670 680 690 700
LIFAGKQLED GRTLSDYNIQ KESTLHLVLR LRGGMQIFVK TLTGKTITLE
710 720 730 740 750
VEPSDTIENV KAKIQDKEGI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST
760
LHLVLRLRGG IQA
Length:763
Mass (Da):85,799
Last modified:August 10, 2010 - v1
Checksum:i5693123AE7B186A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF47806.3.
M33013 Genomic DNA. Translation: AAA28999.1.
M33015 Genomic DNA. Translation: AAA29001.1.
M33122 Genomic DNA. Translation: AAA29007.1.
M22428 Genomic DNA. Translation: AAA28997.1.
PIRiA26087.
RefSeqiNP_001261383.1. NM_001274454.1.
NP_523909.2. NM_079185.4.
NP_728908.1. NM_168043.3.
NP_995994.1. NM_206272.1.
UniGeneiDm.2641.

Genome annotation databases

EnsemblMetazoaiFBtr0073177; FBpp0073034; FBgn0003943.
FBtr0073178; FBpp0073035; FBgn0003943.
FBtr0073179; FBpp0089269; FBgn0003943.
FBtr0333142; FBpp0305348; FBgn0003943.
GeneIDi38456.
KEGGidme:Dmel_CG11624.

Similar proteinsi

Entry informationi

Entry nameiUBIQP_DROME
AccessioniPrimary (citable) accession number: P0CG69
Secondary accession number(s): P68198
, Q0E8I1, Q9VKW6, Q9VQX7, Q9VZL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: September 27, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families