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Protein

Polyubiquitin

Gene

Ubi-p63E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541Activating enzyme
Sitei68 – 681Essential for function
Binding sitei72 – 721Activating enzyme

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: FlyBase
  • larval midgut cell programmed cell death Source: FlyBase
  • male meiosis I Source: FlyBase
  • Notch signaling pathway Source: Reactome
  • phagocytosis Source: FlyBase
  • protein ubiquitination Source: FlyBase
  • spermatid differentiation Source: FlyBase
  • ubiquitin homeostasis Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyubiquitin
Cleaved into the following chain:
Gene namesi
Name:Ubi-p63E
Synonyms:Ubi-m
ORF Names:CG11624
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0003943. Ubi-p63E.

Subcellular locationi

Ubiquitin :

GO - Cellular componenti

  • cytosol Source: Reactome
  • microtubule associated complex Source: FlyBase
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676UbiquitinPRO_0000114813Add
BLAST
Chaini77 – 15276UbiquitinPRO_0000396260Add
BLAST
Chaini153 – 22876UbiquitinPRO_0000396261Add
BLAST
Chaini229 – 30476UbiquitinPRO_0000396262Add
BLAST
Chaini305 – 38076UbiquitinPRO_0000396263Add
BLAST
Chaini381 – 45676UbiquitinPRO_0000396264Add
BLAST
Chaini457 – 53276UbiquitinPRO_0000396265Add
BLAST
Chaini533 – 60876UbiquitinPRO_0000396266Add
BLAST
Chaini609 – 68476UbiquitinPRO_0000396267Add
BLAST
Chaini685 – 76076UbiquitinPRO_0000396268Add
BLAST
Propeptidei761 – 7633PRO_0000396269

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki48 – 48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP0CG69.

Expressioni

Gene expression databases

BgeeiFBgn0003943.
ExpressionAtlasiP0CG69. differential.
GenevisibleiP0CG69. DM.

Interactioni

Protein-protein interaction databases

BioGridi63944. 16 interactions.
STRINGi7227.FBpp0073035.

Structurei

3D structure databases

ProteinModelPortaliP0CG69.
SMRiP0CG69. Positions 1-759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7676Ubiquitin-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini77 – 15276Ubiquitin-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini153 – 22876Ubiquitin-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini229 – 30476Ubiquitin-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini305 – 38076Ubiquitin-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini381 – 45676Ubiquitin-like 6PROSITE-ProRule annotationAdd
BLAST
Domaini457 – 53276Ubiquitin-like 7PROSITE-ProRule annotationAdd
BLAST
Domaini533 – 60876Ubiquitin-like 8PROSITE-ProRule annotationAdd
BLAST
Domaini609 – 68476Ubiquitin-like 9PROSITE-ProRule annotationAdd
BLAST
Domaini685 – 76076Ubiquitin-like 10PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin family.Curated
Contains 10 ubiquitin-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00810000125435.
InParanoidiP0CG69.
KOiK08770.
OMAiCKIEVAS.
OrthoDBiEOG091G178I.
PhylomeDBiP0CG69.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 10 hits.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 10 hits.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 10 hits.
PROSITEiPS00299. UBIQUITIN_1. 10 hits.
PS50053. UBIQUITIN_2. 10 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CG69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE
110 120 130 140 150
NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR
160 170 180 190 200
GGMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK
210 220 230 240 250
QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT
260 270 280 290 300
IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR
310 320 330 340 350
LRGGMQIFVK TLTGKTITLE VEPSDTIENV KAKIQDKEGI PPDQQRLIFA
360 370 380 390 400
GKQLEDGRTL SDYNIQKEST LHLVLRLRGG MQIFVKTLTG KTITLEVEPS
410 420 430 440 450
DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV
460 470 480 490 500
LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI
510 520 530 540 550
FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GGMQIFVKTL TGKTITLEVE
560 570 580 590 600
PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLEDGRTLSD YNIQKESTLH
610 620 630 640 650
LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR
660 670 680 690 700
LIFAGKQLED GRTLSDYNIQ KESTLHLVLR LRGGMQIFVK TLTGKTITLE
710 720 730 740 750
VEPSDTIENV KAKIQDKEGI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST
760
LHLVLRLRGG IQA
Length:763
Mass (Da):85,799
Last modified:August 10, 2010 - v1
Checksum:i5693123AE7B186A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF47806.3.
M33013 Genomic DNA. Translation: AAA28999.1.
M33015 Genomic DNA. Translation: AAA29001.1.
M33122 Genomic DNA. Translation: AAA29007.1.
M22428 Genomic DNA. Translation: AAA28997.1.
PIRiA26087.
RefSeqiNP_001261383.1. NM_001274454.1.
NP_523909.2. NM_079185.4.
NP_728908.1. NM_168043.3.
NP_995994.1. NM_206272.1.
UniGeneiDm.2641.

Genome annotation databases

EnsemblMetazoaiFBtr0073177; FBpp0073034; FBgn0003943.
FBtr0073178; FBpp0073035; FBgn0003943.
FBtr0073179; FBpp0089269; FBgn0003943.
FBtr0333142; FBpp0305348; FBgn0003943.
GeneIDi38456.
KEGGidme:Dmel_CG11624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF47806.3.
M33013 Genomic DNA. Translation: AAA28999.1.
M33015 Genomic DNA. Translation: AAA29001.1.
M33122 Genomic DNA. Translation: AAA29007.1.
M22428 Genomic DNA. Translation: AAA28997.1.
PIRiA26087.
RefSeqiNP_001261383.1. NM_001274454.1.
NP_523909.2. NM_079185.4.
NP_728908.1. NM_168043.3.
NP_995994.1. NM_206272.1.
UniGeneiDm.2641.

3D structure databases

ProteinModelPortaliP0CG69.
SMRiP0CG69. Positions 1-759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63944. 16 interactions.
STRINGi7227.FBpp0073035.

Proteomic databases

PaxDbiP0CG69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073177; FBpp0073034; FBgn0003943.
FBtr0073178; FBpp0073035; FBgn0003943.
FBtr0073179; FBpp0089269; FBgn0003943.
FBtr0333142; FBpp0305348; FBgn0003943.
GeneIDi38456.
KEGGidme:Dmel_CG11624.

Organism-specific databases

CTDi38456.
FlyBaseiFBgn0003943. Ubi-p63E.

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00810000125435.
InParanoidiP0CG69.
KOiK08770.
OMAiCKIEVAS.
OrthoDBiEOG091G178I.
PhylomeDBiP0CG69.

Enzyme and pathway databases

ReactomeiR-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.

Miscellaneous databases

GenomeRNAii38456.
PROiP0CG69.

Gene expression databases

BgeeiFBgn0003943.
ExpressionAtlasiP0CG69. differential.
GenevisibleiP0CG69. DM.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 10 hits.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 10 hits.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 10 hits.
PROSITEiPS00299. UBIQUITIN_1. 10 hits.
PS50053. UBIQUITIN_2. 10 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBIQP_DROME
AccessioniPrimary (citable) accession number: P0CG69
Secondary accession number(s): P68198
, Q0E8I1, Q9VKW6, Q9VQX7, Q9VZL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: September 7, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

In Drosophila ubiquitin is encoded by 3 different genes. RpL40 and RpS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. Ubi-p63E gene codes for a polyubiquitin precursor with 10 exact head to tail repeats.
For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.