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P0CG53

- UBB_BOVIN

UniProt

P0CG53 - UBB_BOVIN

Protein

Polyubiquitin-B

Gene

UBB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541Activating enzyme
    Sitei68 – 681Essential for function
    Binding sitei72 – 721Activating enzyme

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyubiquitin-B
    Cleaved into the following chain:
    Gene namesi
    Name:UBB
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 19

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7676UbiquitinPRO_0000396140Add
    BLAST
    Chaini77 – 15276UbiquitinPRO_0000396141Add
    BLAST
    Chaini153 – 22876UbiquitinPRO_0000396142Add
    BLAST
    Chaini229 – 30476UbiquitinPRO_0000396143Add
    BLAST
    Propeptidei305 – 3051PRO_0000396144

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki6 – 6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki11 – 11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki27 – 27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki29 – 29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki33 – 33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki48 – 48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
    Cross-linki63 – 63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei65 – 651Phosphoserine; by PINK1By similarity
    Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

    Post-translational modificationi

    Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PARK2), triggering mitophagy By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP0CG53.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HGSO149647EBI-5333021,EBI-740220From a different organism.
    HSE1P387532EBI-5333021,EBI-1382From a different organism.
    STAMQ927839EBI-5333021,EBI-752333From a different organism.
    VPS27P403436EBI-5333021,EBI-20380From a different organism.

    Protein-protein interaction databases

    BioGridi158705. 1 interaction.
    IntActiP0CG53. 10 interactions.
    MINTiMINT-1486309.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CG53.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 7676Ubiquitin-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini77 – 15276Ubiquitin-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini153 – 22876Ubiquitin-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini229 – 30476Ubiquitin-like 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin family.Curated
    Contains 4 ubiquitin-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    GeneTreeiENSGT00750000117491.
    KOiK04551.
    OMAiVHENTRR.
    OrthoDBiEOG7JDR1W.
    TreeFamiTF300820.

    Family and domain databases

    InterProiIPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view]
    PfamiPF00240. ubiquitin. 4 hits.
    [Graphical view]
    PRINTSiPR00348. UBIQUITIN.
    SMARTiSM00213. UBQ. 4 hits.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 4 hits.
    PROSITEiPS00299. UBIQUITIN_1. 4 hits.
    PS50053. UBIQUITIN_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CG53-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL    50
    EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE 100
    NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR 150
    GGMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK 200
    QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT 250
    IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR 300
    LRGGC 305
    Length:305
    Mass (Da):34,308
    Last modified:August 10, 2010 - v1
    Checksum:i1E6C7878AE958E68
    GO

    Sequence cautioni

    The sequence BAC56573.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the C-terminal part.
    The sequence BAC56573.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti133 – 1331S → F in CAA79146. (PubMed:8382528)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18245 mRNA. Translation: CAA79146.1.
    AB099044 mRNA. Translation: BAC56534.1.
    AB099083 mRNA. Translation: BAC56573.1. Sequence problems.
    BC114001 mRNA. Translation: AAI14002.1.
    M62429 mRNA. Translation: AAA30720.1.
    PIRiI45964.
    S29853.
    RefSeqiNP_776558.1. NM_174133.2.
    XP_005220351.1. XM_005220294.1.
    XP_005220352.1. XM_005220295.1.
    UniGeneiBt.111403.
    Bt.2878.

    Genome annotation databases

    EnsembliENSBTAT00000022919; ENSBTAP00000022919; ENSBTAG00000017246.
    GeneIDi281370.
    KEGGibta:281370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18245 mRNA. Translation: CAA79146.1 .
    AB099044 mRNA. Translation: BAC56534.1 .
    AB099083 mRNA. Translation: BAC56573.1 . Sequence problems.
    BC114001 mRNA. Translation: AAI14002.1 .
    M62429 mRNA. Translation: AAA30720.1 .
    PIRi I45964.
    S29853.
    RefSeqi NP_776558.1. NM_174133.2.
    XP_005220351.1. XM_005220294.1.
    XP_005220352.1. XM_005220295.1.
    UniGenei Bt.111403.
    Bt.2878.

    3D structure databases

    ProteinModelPortali P0CG53.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 158705. 1 interaction.
    IntActi P0CG53. 10 interactions.
    MINTi MINT-1486309.

    Proteomic databases

    PRIDEi P0CG53.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000022919 ; ENSBTAP00000022919 ; ENSBTAG00000017246 .
    GeneIDi 281370.
    KEGGi bta:281370.

    Organism-specific databases

    CTDi 7314.

    Phylogenomic databases

    GeneTreei ENSGT00750000117491.
    KOi K04551.
    OMAi VHENTRR.
    OrthoDBi EOG7JDR1W.
    TreeFami TF300820.

    Miscellaneous databases

    NextBioi 20805375.

    Family and domain databases

    InterProi IPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view ]
    Pfami PF00240. ubiquitin. 4 hits.
    [Graphical view ]
    PRINTSi PR00348. UBIQUITIN.
    SMARTi SM00213. UBQ. 4 hits.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 4 hits.
    PROSITEi PS00299. UBIQUITIN_1. 4 hits.
    PS50053. UBIQUITIN_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a full-length cDNA encoding a bovine four tandem-repeat ubiquitin."
      Wempe F., Scheit K.H.
      Biochim. Biophys. Acta 1172:209-211(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Seminal vesicle.
    2. "Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray."
      Ishiwata H., Katsuma S., Kizaki K., Patel O.V., Nakano H., Takahashi T., Imai K., Hirasawa A., Shiojima S., Ikawa H., Suzuki Y., Tsujimoto G., Izaike Y., Todoroki J., Hashizume K.
      Mol. Reprod. Dev. 65:9-18(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum and Liver.
    4. "The complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells."
      Schlesinger D.H., Goldstein G., Niall H.D.
      Biochemistry 14:2214-2218(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-74.
    5. "The biosynthesis of ubiquitin by parathyroid gland."
      Hamilton J.W., Rouse J.B.
      Biochem. Biophys. Res. Commun. 96:114-120(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-50.
    6. "Ganglioside binding proteins of calf brain with ubiquitin-like N-terminals."
      Zdebska E., Antoniewicz J., Nilsson B., Sandhoff K., Fuerst W., Janik P., Koscielak J.
      Eur. J. Biochem. 210:483-489(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20.
      Tissue: Brain.
    7. "Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences."
      Meyers G., Tautz N., Dubovi E.J., Thiel H.-J.
      Virology 180:602-616(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 142-305.
      Tissue: Kidney.

    Entry informationi

    Entry nameiUBB_BOVIN
    AccessioniPrimary (citable) accession number: P0CG53
    Secondary accession number(s): O97577
    , P02248, P02249, P02250, P62990, P80169, Q01235, Q24K23, Q28169, Q28170, Q29120, Q3T0V5, Q3ZCE3, Q862C1, Q862F4, Q862M4, Q862T5, Q862X8, Q91887, Q91888
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Ubiquitin is encoded by 4 different genes. Uba52 and Rps27a genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.
    For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3