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P0CG49

- UBB_MOUSE

UniProt

P0CG49 - UBB_MOUSE

Protein

Polyubiquitin-B

Gene

Ubb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541Activating enzyme
    Sitei68 – 681Essential for function
    Binding sitei72 – 721Activating enzyme

    GO - Biological processi

    1. adipose tissue development Source: MGI
    2. energy homeostasis Source: MGI
    3. fat pad development Source: MGI
    4. female gonad development Source: MGI
    5. female meiosis I Source: MGI
    6. hypothalamus gonadotrophin-releasing hormone neuron development Source: MGI
    7. male gonad development Source: MGI
    8. male meiosis I Source: MGI
    9. seminiferous tubule development Source: MGI
    10. ubiquitin homeostasis Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.
    REACT_24972. Circadian Clock.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyubiquitin-B
    Cleaved into the following chain:
    Gene namesi
    Name:Ubb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:98888. Ubb.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7676UbiquitinPRO_0000114801Add
    BLAST
    Chaini77 – 15276UbiquitinPRO_0000396188Add
    BLAST
    Chaini153 – 22876UbiquitinPRO_0000396189Add
    BLAST
    Chaini229 – 30476UbiquitinPRO_0000396190Add
    BLAST
    Propeptidei305 – 3051PRO_0000396191

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki6 – 6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki11 – 11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki27 – 27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki29 – 29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki33 – 33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki48 – 48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki63 – 63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei65 – 651Phosphoserine; by PINK1By similarity
    Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

    Post-translational modificationi

    Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PARK2), triggering mitophagy By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP0CG49.

    2D gel databases

    REPRODUCTION-2DPAGEP62991.

    PTM databases

    PhosphoSiteiP0CG49.

    Expressioni

    Gene expression databases

    BgeeiP0CG49.
    GenevestigatoriP62991.

    Interactioni

    Protein-protein interaction databases

    BioGridi204402. 13 interactions.
    IntActiP0CG49. 2 interactions.
    MINTiMINT-8372791.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CG49.
    SMRiP0CG49. Positions 1-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 7676Ubiquitin-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini77 – 15276Ubiquitin-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini153 – 22876Ubiquitin-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini229 – 30476Ubiquitin-like 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin family.Curated
    Contains 4 ubiquitin-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    KOiK04551.
    OMAiVHENTRR.
    OrthoDBiEOG7JDR1W.
    PhylomeDBiP0CG49.
    TreeFamiTF300820.

    Family and domain databases

    InterProiIPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view]
    PfamiPF00240. ubiquitin. 4 hits.
    [Graphical view]
    PRINTSiPR00348. UBIQUITIN.
    SMARTiSM00213. UBQ. 4 hits.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 4 hits.
    PROSITEiPS00299. UBIQUITIN_1. 4 hits.
    PS50053. UBIQUITIN_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CG49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL    50
    EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE 100
    NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR 150
    GGMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK 200
    QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVEPSDT 250
    IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR 300
    LRGGY 305
    Length:305
    Mass (Da):34,369
    Last modified:August 10, 2010 - v1
    Checksum:i0B8C7878AE958E68
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311Q → R in BAB29028. (PubMed:16141072)Curated
    Sequence conflicti54 – 541R → S in BAB29028. (PubMed:16141072)Curated
    Sequence conflicti61 – 611I → N in BAB29028. (PubMed:16141072)Curated
    Sequence conflicti92 – 921E → G in BAB29028. (PubMed:16141072)Curated
    Sequence conflicti117 – 1171Q → H in AAH19850. (PubMed:15489334)Curated
    Sequence conflicti147 – 1471L → F in BAB28242. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51703 mRNA. Translation: CAA35999.1.
    AK012443 mRNA. Translation: BAB28242.1.
    AK003190 mRNA. Translation: BAB22630.1.
    AK013873 mRNA. Translation: BAB29028.1.
    BC019850 mRNA. Translation: AAH19850.1.
    CCDSiCCDS24826.1.
    PIRiS12583.
    RefSeqiNP_035794.1. NM_011664.3.
    XP_006533222.1. XM_006533159.1.
    UniGeneiMm.282093.
    Mm.371592.

    Genome annotation databases

    EnsembliENSMUST00000019649; ENSMUSP00000019649; ENSMUSG00000019505.
    GeneIDi22187.
    KEGGimmu:22187.
    UCSCiuc007jjg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51703 mRNA. Translation: CAA35999.1 .
    AK012443 mRNA. Translation: BAB28242.1 .
    AK003190 mRNA. Translation: BAB22630.1 .
    AK013873 mRNA. Translation: BAB29028.1 .
    BC019850 mRNA. Translation: AAH19850.1 .
    CCDSi CCDS24826.1.
    PIRi S12583.
    RefSeqi NP_035794.1. NM_011664.3.
    XP_006533222.1. XM_006533159.1.
    UniGenei Mm.282093.
    Mm.371592.

    3D structure databases

    ProteinModelPortali P0CG49.
    SMRi P0CG49. Positions 1-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204402. 13 interactions.
    IntActi P0CG49. 2 interactions.
    MINTi MINT-8372791.

    PTM databases

    PhosphoSitei P0CG49.

    2D gel databases

    REPRODUCTION-2DPAGE P62991.

    Proteomic databases

    PRIDEi P0CG49.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019649 ; ENSMUSP00000019649 ; ENSMUSG00000019505 .
    GeneIDi 22187.
    KEGGi mmu:22187.
    UCSCi uc007jjg.1. mouse.

    Organism-specific databases

    CTDi 7314.
    MGIi MGI:98888. Ubb.

    Phylogenomic databases

    KOi K04551.
    OMAi VHENTRR.
    OrthoDBi EOG7JDR1W.
    PhylomeDBi P0CG49.
    TreeFami TF300820.

    Enzyme and pathway databases

    Reactomei REACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.
    REACT_24972. Circadian Clock.

    Miscellaneous databases

    ChiTaRSi UBB. mouse.
    NextBioi 302157.
    PROi P0CG49.
    SOURCEi Search...

    Gene expression databases

    Bgeei P0CG49.
    Genevestigatori P62991.

    Family and domain databases

    InterProi IPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view ]
    Pfami PF00240. ubiquitin. 4 hits.
    [Graphical view ]
    PRINTSi PR00348. UBIQUITIN.
    SMARTi SM00213. UBQ. 4 hits.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 4 hits.
    PROSITEi PS00299. UBIQUITIN_1. 4 hits.
    PS50053. UBIQUITIN_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Head and Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and FVB/N.
      Tissue: Liver and Mammary tumor.
    4. "The emerging complexity of protein ubiquitination."
      Komander D.
      Biochem. Soc. Trans. 37:937-953(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, FUNCTION.

    Entry informationi

    Entry nameiUBB_MOUSE
    AccessioniPrimary (citable) accession number: P0CG49
    Secondary accession number(s): P02248
    , P02249, P02250, P62991, Q29120, Q62317, Q64223, Q8VCH1, Q91887, Q91888, Q9CXY4, Q9CZM0, Q9D1R5, Q9D8D9, Q9ET23, Q9ET24, Q9Z0H9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Ubiquitin is encoded by 4 different genes. Uba52 and Rps27a genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.
    For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3