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Protein

Chromosome transmission fidelity protein 8 homolog

Gene

CHTF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated the presence of primed DNA, replication protein A (RPA) and proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates POLH, which is suggestive of a protein network that coordinates DNA repair, recombination and chromosome cohesion reactions with replication fork progression.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 8 homolog
Short name:
hCTF8
Gene namesi
Name:CHTF8
Synonyms:CTF8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:24353. CHTF8.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Associates with chromatin during S phase.

GO - Cellular componenti

  • Ctf18 RFC-like complex Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000168802.
PharmGKBiPA164717934.

Polymorphism and mutation databases

BioMutaiHAS3.
DMDMi298351632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003272531 – 121Chromosome transmission fidelity protein 8 homologAdd BLAST121

Proteomic databases

EPDiP0CG13.
MaxQBiP0CG13.
PaxDbiP0CG13.
PeptideAtlasiP0CG13.
PRIDEiP0CG13.

PTM databases

iPTMnetiP0CG13.
PhosphoSitePlusiP0CG13.

Expressioni

Tissue specificityi

Ubiquitously expressed, with abundant expression in kidney, skeletal muscle, testis, liver, ovary, and heart and moderate expression in prostate. Expression is significantly reduced in renal and prostate tumors. No differential expression in breast cancer cells, between lobular carcinoma and normal lobules.1 Publication

Gene expression databases

BgeeiENSG00000168802.
ExpressionAtlasiP0CG13. baseline and differential.
GenevisibleiP0CG13. HS.

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex does not interact with the Rad9/Rad1/Hus1 complex. The CTF18-RFC complex interacts with POLH. CTF18/CTF8/DCC1 associate with PCNA. CTF8 exists as a dimer with DCC1.3 Publications

Protein-protein interaction databases

BioGridi120262. 17 interactors.
IntActiP0CG13. 1 interactor.
MINTiMINT-1694396.
STRINGi9606.ENSP00000381290.

Structurei

3D structure databases

ProteinModelPortaliP0CG13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF8 family.Curated

Phylogenomic databases

eggNOGiENOG410J3HT. Eukaryota.
ENOG4111SBI. LUCA.
GeneTreeiENSGT00400000025006.
KOiK11270.
TreeFamiTF314676.

Family and domain databases

InterProiIPR018607. Ctf8.
[Graphical view]
PfamiPF09696. Ctf8. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P0CG13-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQIVISSAR AGGLAEWVLM ELQGEIEARY STGLAGNLLG DLHYTTEGIP
60 70 80 90 100
VLIVGHHILY GKIIHLEKPF AVLVKHTPGD QDCDELGRET GTRYLVTALI
110 120
KDKILFKTRP KPIITSVPKK V
Length:121
Mass (Da):13,314
Last modified:June 15, 2010 - v1
Checksum:i17F58496A479DA3F
GO
Isoform 2 (identifier: P0CG12-1) [UniParc]FASTAAdd to basket
Also known as: DERPC
The sequence of this isoform can be found in the external entry P0CG12.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:524
Mass (Da):51,391
GO

Sequence cautioni

The sequence AAQ08897 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
The sequence BAA91144 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
The sequence EAW83250 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF525164 mRNA. Translation: AAQ08897.1. Sequence problems.
AK000407 mRNA. Translation: BAA91144.1. Sequence problems.
AC009027 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83250.1. Sequence problems.
BC018700 mRNA. Translation: AAH18700.4.
CCDSiCCDS42185.1. [P0CG13-1]
RefSeqiNP_001034779.1. NM_001039690.3. [P0CG13-1]
NP_001035236.1. NM_001040146.3. [P0CG13-1]
XP_011521469.1. XM_011523167.1.
XP_011521470.1. XM_011523168.1.
XP_016878859.1. XM_017023370.1. [P0CG13-1]
UniGeneiHs.85962.

Genome annotation databases

EnsembliENST00000398235; ENSP00000381290; ENSG00000168802. [P0CG13-1]
ENST00000448552; ENSP00000408367; ENSG00000168802. [P0CG13-1]
GeneIDi54921.
KEGGihsa:54921.
UCSCiuc002ewn.3. human. [P0CG13-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF525164 mRNA. Translation: AAQ08897.1. Sequence problems.
AK000407 mRNA. Translation: BAA91144.1. Sequence problems.
AC009027 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83250.1. Sequence problems.
BC018700 mRNA. Translation: AAH18700.4.
CCDSiCCDS42185.1. [P0CG13-1]
RefSeqiNP_001034779.1. NM_001039690.3. [P0CG13-1]
NP_001035236.1. NM_001040146.3. [P0CG13-1]
XP_011521469.1. XM_011523167.1.
XP_011521470.1. XM_011523168.1.
XP_016878859.1. XM_017023370.1. [P0CG13-1]
UniGeneiHs.85962.

3D structure databases

ProteinModelPortaliP0CG13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120262. 17 interactors.
IntActiP0CG13. 1 interactor.
MINTiMINT-1694396.
STRINGi9606.ENSP00000381290.

PTM databases

iPTMnetiP0CG13.
PhosphoSitePlusiP0CG13.

Polymorphism and mutation databases

BioMutaiHAS3.
DMDMi298351632.

Proteomic databases

EPDiP0CG13.
MaxQBiP0CG13.
PaxDbiP0CG13.
PeptideAtlasiP0CG13.
PRIDEiP0CG13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398235; ENSP00000381290; ENSG00000168802. [P0CG13-1]
ENST00000448552; ENSP00000408367; ENSG00000168802. [P0CG13-1]
GeneIDi54921.
KEGGihsa:54921.
UCSCiuc002ewn.3. human. [P0CG13-1]

Organism-specific databases

CTDi54921.
GeneCardsiCHTF8.
HGNCiHGNC:24353. CHTF8.
MIMi613202. gene.
neXtProtiNX_P0CG13.
OpenTargetsiENSG00000168802.
PharmGKBiPA164717934.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J3HT. Eukaryota.
ENOG4111SBI. LUCA.
GeneTreeiENSGT00400000025006.
KOiK11270.
TreeFamiTF314676.

Enzyme and pathway databases

BioCyciZFISH:G66-33093-MONOMER.

Miscellaneous databases

ChiTaRSiCHTF8. human.
GeneWikiiCTF8.
GenomeRNAii54921.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168802.
ExpressionAtlasiP0CG13. baseline and differential.
GenevisibleiP0CG13. HS.

Family and domain databases

InterProiIPR018607. Ctf8.
[Graphical view]
PfamiPF09696. Ctf8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTF8_HUMAN
AccessioniPrimary (citable) accession number: P0CG13
Secondary accession number(s): A8MYX8
, Q71E72, Q8NDH8, Q8WV66, Q9NX73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

mRNA expression studied by PubMed:12477976 does not differentiate between isoform 1 and isoform 2.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.