Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chromosome transmission fidelity protein 8 homolog

Gene

CHTF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated the presence of primed DNA, replication protein A (RPA) and proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates POLH, which is suggestive of a protein network that coordinates DNA repair, recombination and chromosome cohesion reactions with replication fork progression.2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 8 homolog
Short name:
hCTF8
Gene namesi
Name:CHTF8
Synonyms:CTF8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:24353. CHTF8.

Subcellular locationi

Nucleus 1 Publication
Note: Associates with chromatin during S phase.

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164717934.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Chromosome transmission fidelity protein 8 homologPRO_0000327253Add
BLAST

Proteomic databases

MaxQBiP0CG13.
PRIDEiP0CG13.

PTM databases

PhosphoSiteiP0CG13.

Expressioni

Tissue specificityi

Ubiquitously expressed, with abundant expression in kidney, skeletal muscle, testis, liver, ovary, and heart and moderate expression in prostate. Expression is significantly reduced in renal and prostate tumors. No differential expression in breast cancer cells, between lobular carcinoma and normal lobules.1 Publication

Gene expression databases

BgeeiP0CG13.
ExpressionAtlasiP0CG13. baseline.
GenevestigatoriQ8WV66.

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex does not interact with the Rad9/Rad1/Hus1 complex. The CTF18-RFC complex interacts with POLH. CTF18/CTF8/DCC1 associate with PCNA. CTF8 exists as a dimer with DCC1.3 Publications

Protein-protein interaction databases

BioGridi120262. 11 interactions.
IntActiP0CG13. 1 interaction.
MINTiMINT-1694396.

Structurei

3D structure databases

ProteinModelPortaliP0CG13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF8 family.Curated

Phylogenomic databases

GeneTreeiENSGT00400000025006.
KOiK11270.
TreeFamiTF314676.

Family and domain databases

InterProiIPR018607. Chromosome_trans_fidel_Ctf8.
[Graphical view]
PfamiPF09696. Ctf8. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P0CG13-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQIVISSAR AGGLAEWVLM ELQGEIEARY STGLAGNLLG DLHYTTEGIP
60 70 80 90 100
VLIVGHHILY GKIIHLEKPF AVLVKHTPGD QDCDELGRET GTRYLVTALI
110 120
KDKILFKTRP KPIITSVPKK V
Length:121
Mass (Da):13,314
Last modified:June 14, 2010 - v1
Checksum:i17F58496A479DA3F
GO
Isoform 2 (identifier: P0CG12-1) [UniParc]FASTAAdd to basket

Also known as: DERPC

The sequence of this isoform can be found in the external entry P0CG12.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:524
Mass (Da):51,391
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF525164 mRNA. Translation: AAQ08897.1. Sequence problems.
AK000407 mRNA. Translation: BAA91144.1. Sequence problems.
AC009027 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83250.1. Sequence problems.
BC018700 mRNA. Translation: AAH18700.4.
CCDSiCCDS42185.1. [P0CG13-1]
RefSeqiNP_001034779.1. NM_001039690.3. [P0CG13-1]
NP_001035236.1. NM_001040146.3. [P0CG13-1]
UniGeneiHs.85962.

Genome annotation databases

EnsembliENST00000398235; ENSP00000381290; ENSG00000168802. [P0CG13-1]
ENST00000448552; ENSP00000408367; ENSG00000168802. [P0CG13-1]
GeneIDi54921.
KEGGihsa:54921.
UCSCiuc002ewn.2. human. [P0CG13-1]

Polymorphism databases

DMDMi298351632.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF525164 mRNA. Translation: AAQ08897.1. Sequence problems.
AK000407 mRNA. Translation: BAA91144.1. Sequence problems.
AC009027 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83250.1. Sequence problems.
BC018700 mRNA. Translation: AAH18700.4.
CCDSiCCDS42185.1. [P0CG13-1]
RefSeqiNP_001034779.1. NM_001039690.3. [P0CG13-1]
NP_001035236.1. NM_001040146.3. [P0CG13-1]
UniGeneiHs.85962.

3D structure databases

ProteinModelPortaliP0CG13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120262. 11 interactions.
IntActiP0CG13. 1 interaction.
MINTiMINT-1694396.

PTM databases

PhosphoSiteiP0CG13.

Polymorphism databases

DMDMi298351632.

Proteomic databases

MaxQBiP0CG13.
PRIDEiP0CG13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398235; ENSP00000381290; ENSG00000168802. [P0CG13-1]
ENST00000448552; ENSP00000408367; ENSG00000168802. [P0CG13-1]
GeneIDi54921.
KEGGihsa:54921.
UCSCiuc002ewn.2. human. [P0CG13-1]

Organism-specific databases

CTDi54921.
GeneCardsiGC16M069151.
HGNCiHGNC:24353. CHTF8.
MIMi613202. gene.
neXtProtiNX_P0CG13.
PharmGKBiPA164717934.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00400000025006.
KOiK11270.
TreeFamiTF314676.

Miscellaneous databases

ChiTaRSiCHTF8. human.
GeneWikiiCTF8.
GenomeRNAii54921.
NextBioi57996.
PROiP0CG13.
SOURCEiSearch...

Gene expression databases

BgeeiP0CG13.
ExpressionAtlasiP0CG13. baseline.
GenevestigatoriQ8WV66.

Family and domain databases

InterProiIPR018607. Chromosome_trans_fidel_Ctf8.
[Graphical view]
PfamiPF09696. Ctf8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human novel gene DERPC on 16q22.1 inhibits prostate tumor cell growth and its expression is decreased in prostate and renal tumors."
    Sun M., Ma L., Xu L., Li J., Zhang W., Petrovics G., Makarem M., Sesterhenn I., Zhang M., Blanchette-Mackie E.J., Moul J., Srivastava S., Zou Z.
    Mol. Med. 8:655-663(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  6. "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment."
    Merkle C.J., Karnitz L.M., Henry-Sanchez J.T., Chen J.
    J. Biol. Chem. 278:30051-30056(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX, SUBCELLULAR LOCATION.
  7. "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA."
    Bermudez V.P., Maniwa Y., Tappin I., Ozato K., Yokomori K., Hurwitz J.
    Proc. Natl. Acad. Sci. U.S.A. 100:10237-10242(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE CTF18-RFC COMPLEX, INTERACTION WITH DCC1, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity."
    Shiomi Y., Masutani C., Hanaoka F., Kimura H., Tsurimoto T.
    J. Biol. Chem. 282:20906-20914(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE CTF18-RFC COMPLEX, INTERACTION OF THE CTF18-RFC COMPLEX WITH POLH, IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCTF8_HUMAN
AccessioniPrimary (citable) accession number: P0CG13
Secondary accession number(s): A8MYX8
, Q71E72, Q8NDH8, Q8WV66, Q9NX73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 14, 2010
Last sequence update: June 14, 2010
Last modified: March 3, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

mRNA expression studied by PubMed:12477976 does not differentiate between isoform 1 and isoform 2.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.