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P0CG13

- CTF8_HUMAN

UniProt

P0CG13 - CTF8_HUMAN

Protein

Chromosome transmission fidelity protein 8 homolog

Gene

CHTF8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated the presence of primed DNA, replication protein A (RPA) and proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates POLH, which is suggestive of a protein network that coordinates DNA repair, recombination and chromosome cohesion reactions with replication fork progression.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. DNA replication Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromosome transmission fidelity protein 8 homolog
    Short name:
    hCTF8
    Gene namesi
    Name:CHTF8
    Synonyms:CTF8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:24353. CHTF8.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Associates with chromatin during S phase.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA164717934.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 121121Chromosome transmission fidelity protein 8 homologPRO_0000327253Add
    BLAST

    Proteomic databases

    MaxQBiP0CG13.
    PRIDEiP0CG13.

    PTM databases

    PhosphoSiteiP0CG13.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed, with abundant expression in kidney, skeletal muscle, testis, liver, ovary, and heart and moderate expression in prostate. Expression is significantly reduced in renal and prostate tumors. No differential expression in breast cancer cells, between lobular carcinoma and normal lobules.1 Publication

    Gene expression databases

    ArrayExpressiP0CG13.
    BgeeiP0CG13.
    GenevestigatoriQ8WV66.

    Interactioni

    Subunit structurei

    Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex does not interact with the Rad9/Rad1/Hus1 complex. The CTF18-RFC complex interacts with POLH. CTF18/CTF8/DCC1 associate with PCNA. CTF8 exists as a dimer with DCC1.3 Publications

    Protein-protein interaction databases

    BioGridi120262. 8 interactions.
    IntActiP0CG13. 1 interaction.
    MINTiMINT-1694396.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CG13.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CTF8 family.Curated

    Phylogenomic databases

    KOiK11270.
    TreeFamiTF314676.

    Family and domain databases

    InterProiIPR018607. Chromosome_trans_fidel_Ctf8.
    [Graphical view]
    PfamiPF09696. Ctf8. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P0CG13-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVQIVISSAR AGGLAEWVLM ELQGEIEARY STGLAGNLLG DLHYTTEGIP    50
    VLIVGHHILY GKIIHLEKPF AVLVKHTPGD QDCDELGRET GTRYLVTALI 100
    KDKILFKTRP KPIITSVPKK V 121
    Length:121
    Mass (Da):13,314
    Last modified:June 15, 2010 - v1
    Checksum:i17F58496A479DA3F
    GO
    Isoform 2 (identifier: P0CG12-1) [UniParc]FASTAAdd to Basket

    Also known as: DERPC

    The sequence of this isoform can be found in the external entry P0CG12.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:524
    Mass (Da):51,391
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF525164 mRNA. Translation: AAQ08897.1. Sequence problems.
    AK000407 mRNA. Translation: BAA91144.1. Sequence problems.
    AC009027 Genomic DNA. No translation available.
    CH471092 Genomic DNA. Translation: EAW83250.1. Sequence problems.
    BC018700 mRNA. Translation: AAH18700.4.
    CCDSiCCDS42185.1. [P0CG13-1]
    RefSeqiNP_001034779.1. NM_001039690.3. [P0CG13-1]
    NP_001035236.1. NM_001040146.3. [P0CG13-1]
    UniGeneiHs.85962.

    Genome annotation databases

    EnsembliENST00000398235; ENSP00000381290; ENSG00000168802. [P0CG13-1]
    ENST00000448552; ENSP00000408367; ENSG00000168802. [P0CG13-1]
    GeneIDi54921.
    KEGGihsa:54921.
    UCSCiuc002ewn.2. human. [P0CG13-1]

    Polymorphism databases

    DMDMi298351632.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF525164 mRNA. Translation: AAQ08897.1 . Sequence problems.
    AK000407 mRNA. Translation: BAA91144.1 . Sequence problems.
    AC009027 Genomic DNA. No translation available.
    CH471092 Genomic DNA. Translation: EAW83250.1 . Sequence problems.
    BC018700 mRNA. Translation: AAH18700.4 .
    CCDSi CCDS42185.1. [P0CG13-1 ]
    RefSeqi NP_001034779.1. NM_001039690.3. [P0CG13-1 ]
    NP_001035236.1. NM_001040146.3. [P0CG13-1 ]
    UniGenei Hs.85962.

    3D structure databases

    ProteinModelPortali P0CG13.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120262. 8 interactions.
    IntActi P0CG13. 1 interaction.
    MINTi MINT-1694396.

    PTM databases

    PhosphoSitei P0CG13.

    Polymorphism databases

    DMDMi 298351632.

    Proteomic databases

    MaxQBi P0CG13.
    PRIDEi P0CG13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000398235 ; ENSP00000381290 ; ENSG00000168802 . [P0CG13-1 ]
    ENST00000448552 ; ENSP00000408367 ; ENSG00000168802 . [P0CG13-1 ]
    GeneIDi 54921.
    KEGGi hsa:54921.
    UCSCi uc002ewn.2. human. [P0CG13-1 ]

    Organism-specific databases

    CTDi 54921.
    GeneCardsi GC16M069151.
    HGNCi HGNC:24353. CHTF8.
    MIMi 613202. gene.
    neXtProti NX_P0CG13.
    PharmGKBi PA164717934.
    GenAtlasi Search...

    Phylogenomic databases

    KOi K11270.
    TreeFami TF314676.

    Miscellaneous databases

    GeneWikii CTF8.
    GenomeRNAii 54921.
    NextBioi 57996.
    PROi P0CG13.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P0CG13.
    Bgeei P0CG13.
    Genevestigatori Q8WV66.

    Family and domain databases

    InterProi IPR018607. Chromosome_trans_fidel_Ctf8.
    [Graphical view ]
    Pfami PF09696. Ctf8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A human novel gene DERPC on 16q22.1 inhibits prostate tumor cell growth and its expression is decreased in prostate and renal tumors."
      Sun M., Ma L., Xu L., Li J., Zhang W., Petrovics G., Makarem M., Sesterhenn I., Zhang M., Blanchette-Mackie E.J., Moul J., Srivastava S., Zou Z.
      Mol. Med. 8:655-663(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    6. "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment."
      Merkle C.J., Karnitz L.M., Henry-Sanchez J.T., Chen J.
      J. Biol. Chem. 278:30051-30056(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX, SUBCELLULAR LOCATION.
    7. "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA."
      Bermudez V.P., Maniwa Y., Tappin I., Ozato K., Yokomori K., Hurwitz J.
      Proc. Natl. Acad. Sci. U.S.A. 100:10237-10242(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE CTF18-RFC COMPLEX, INTERACTION WITH DCC1, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity."
      Shiomi Y., Masutani C., Hanaoka F., Kimura H., Tsurimoto T.
      J. Biol. Chem. 282:20906-20914(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE CTF18-RFC COMPLEX, INTERACTION OF THE CTF18-RFC COMPLEX WITH POLH, IDENTIFICATION BY MASS SPECTROMETRY.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCTF8_HUMAN
    AccessioniPrimary (citable) accession number: P0CG13
    Secondary accession number(s): A8MYX8
    , Q71E72, Q8NDH8, Q8WV66, Q9NX73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    mRNA expression studied by PubMed:12477976 does not differentiate between isoform 1 and isoform 2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3